Rh7CG127200

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0011559
Physical Location & Seq
Forward (+)
9892621 .. 9895268
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG127200.1

Sequence Viewer

Length: 1443 bp
ATGGAAGGACTAGAAGGTAACACAAACAACAATGAAGCTGGTTTGAGCATTGAACAAGCTCGAACAAAGTGTGATCAAGATCATGAATATACACTCCCCTCAGCTCACGCTATTGGTCATGATTCATGGCAGCAGGTTGGGTTGATGATGGTGACTGGCTTCAATTGCGGGTACATATTGAGCTTTTCAAATCTGATGCTTGTGCCTTTGGGTTGGACATGGGGTATCATCTGCCTCGTTGTTGTTGGCTTTTATACCGCCTATGCTAACTGGCTTTTGGCTGCTTTTCACTTCATCAATGGCCAAAGATTCATCAGATACAGAGATATTATGGGCTATTTATATGGCAGAGAAATGTATTACATAACATGGGTTTTACAGTTCTCGACCCTACTTCTTGGAAATATGGGTTTCATTCTTCTAGGGGGTAGAGCACTCAAGGAGATTAACTCAGAACTCAGTGATTCTCCACTGAGGCTCCAATACTTTATTGTCATCACCGGAGTGACCTACTTCATATTCGCCTTTTTCATTCCAACAATGTCTAAAATGAGATTTTGGCTTGGACCCTCTACCATTGTCACTTTTGCTTACATTGTCATACTCTTGTTGGTACTAGTTAAAGATGGGAAAACAAAGATGACAGAGCGAAATTATGAAATCCATGGAAACAAAGCTGAGAGAGTGTTCAATGCCTTCAATGCAGTTTCAGCTATTATTGTCTGCAACACCGCAGGCTTGCTACTAGAAATACAGTCAACCCTGCAGAAGCCAGCAGTAACAAATATGAGGAAAGCTTTATATCTGCAGTATAGTGCAGGGCTCTTATTTTACTATGGAGTCAGCATAGTAGGATACTGGGCATATGGCGCAACCGTATCTGAATACCTTCCTGAAGAGCTAAGTGGTCCTAAATCGGTTAAGGTCCTCATAAACGCTGCCGTGTTTCTACAATCCATAGTCTCACAACATGTAAGAATGATCCATCTTCTTGTTTGTCAAACACTTCACTGCAAAAGACTCGTTTTTCTACTGAACTACTTCAATTTTTACCATCAACAGATGTTTGTTGCACCAATTCATGAGGCCCTTGATACCAAGTTCCTTAAACTTGATGAGAGCATGCACTGGAAATATAACTTCAAGCGGAGGTTCTACCTCCGAGCCCTTCTCTTCATGGGAAATACATTCGTGACAGCAGCTATTCCTTTCATGGGGGACTTTGTGAACTTATTTGGATCGTTTACACTCGTTCCTCTAACTTTTGTTTTTCCAAGCATGATTTTCATTAAGGTGAAGGGAAAGACAGCTAGACTGGAGAAAAATTTGTGGCATTGGTTCAACATTGTTATTTTTTCTCTGCTTGCGATTATAACTACGATTTCTGCAGTTCGTTCGATTGTCAACAATGTTAAGGAATACAATTTCTTTGCAGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

480

Amino Acids

55.01

Weight (kDa)

9.14

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 41 - 463 5e-52 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1373
Acc36I ACCTGC 1 cut(s) 124
AciI CCGC 4 cut(s) 168, 258, 732, 1147
AclWI GGATC 2 cut(s) 976, 1246
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 1 cut(s) 1324
AcuI CTGAAG 1 cut(s) 915
AfaI GTAC 2 cut(s) 173, 615
AfiI CCNNNNNNNGG 1 cut(s) 1214
AflIII ACRYGT 1 cut(s) 970
AgsI TTSAA 8 cut(s) 53, 163, 189, 691, 700, 1045, 1144, 1342
AhlI ACTAGT 1 cut(s) 616
AleI CACNNNNGTG 1 cut(s) 503
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 1 cut(s) 967
AlwI GGATC 2 cut(s) 976, 1246
AoxI GGCC 2 cut(s) 301, 1086
ApeKI GCWGC 4 cut(s) 130, 281, 938, 1199
ApoI RAATTY 1 cut(s) 1324
Asp700I GAANNNNTTC 2 cut(s) 888, 1040
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 4 cut(s) 566, 908, 925, 1087
AsuHPI GGTGA 3 cut(s) 163, 490, 1306
AvaII GGWCC 3 cut(s) 566, 908, 925
BalI TGGCCA 1 cut(s) 303
BanII GRGCYC 2 cut(s) 825, 1168
Bbv12I GWGCWC 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 4 cut(s) 142, 268, 925, 1211
BccI CCATC 4 cut(s) 142, 620, 993, 1062
BceAI ACGGC 1 cut(s) 926
BcgI CGANNNNNNTGC 4 cut(s) 1003, 1037, 1377, 1411
BciVI GTATCC 1 cut(s) 848
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 967
BcuI ACTAGT 1 cut(s) 616
BfaI CTAG 5 cut(s) 11, 422, 617, 746, 1311
BfmI CTRYAG 3 cut(s) 764, 806, 1386
BfuAI ACCTGC 1 cut(s) 124
BfuI GTATCC 1 cut(s) 848
BisI GCNGC 4 cut(s) 131, 282, 939, 1200
BlsI GCNGC 4 cut(s) 132, 283, 940, 1201
Bme18I GGWCC 3 cut(s) 566, 908, 925
BmgT120I GGNCC 4 cut(s) 566, 908, 925, 1087
BmiI GGNNCC 2 cut(s) 479, 568
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 868
BplI GAGNNNNNCTC 4 cut(s) 434, 466, 1155, 1187
BpmI CTGGAG 1 cut(s) 1337
Bpu10I CCTNAGC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 664
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 1214
Bse1I ACTGG 5 cut(s) 160, 275, 863, 1133, 1320
Bse8I GATNNNNATC 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 664
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 1214
BseMII CTCAG 5 cut(s) 114, 464, 465, 472, 669
BseNI ACTGG 5 cut(s) 160, 275, 863, 1133, 1320
BseXI GCAGC 4 cut(s) 142, 268, 925, 1211
BsgI GTGCAG 1 cut(s) 837
BshFI GGCC 2 cut(s) 303, 1088
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 1232
BslI CCNNNNNNNGG 1 cut(s) 1214
BsmAI GTCTC 1 cut(s) 967
BsmFI GGGAC 1 cut(s) 1232
BsnI GGCC 2 cut(s) 303, 1088
Bsp1286I GDGCHC 3 cut(s) 436, 825, 1168
Bsp143I GATC 4 cut(s) 73, 79, 981, 1238
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 4 cut(s) 168, 258, 732, 1147
BspANI GGCC 2 cut(s) 303, 1088
BspCNI CTCAG 5 cut(s) 113, 464, 465, 471, 670
BspHI TCATGA 3 cut(s) 82, 118, 1081
BspLI GGNNCC 2 cut(s) 479, 568
BspMAI CTGCAG 3 cut(s) 768, 810, 1390
BspMI ACCTGC 1 cut(s) 124
BspPI GGATC 2 cut(s) 976, 1246
BspQI GCTCTTC 1 cut(s) 891
BsrI ACTGG 5 cut(s) 160, 275, 863, 1133, 1320
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 4 cut(s) 73, 79, 981, 1238
BssT1I CCWWGG 1 cut(s) 664
Bst4CI ACNGT 3 cut(s) 381, 756, 877
Bst6I CTCTTC 2 cut(s) 891, 1178
BstC8I GCNNGC 5 cut(s) 736, 740, 774, 1124, 1365
BstDEI CTNAG 6 cut(s) 100, 451, 458, 473, 678, 902
BstDSI CCRYGG 1 cut(s) 664
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 4 cut(s) 76, 82, 984, 1241
BstMAI GTCTC 1 cut(s) 967
BstMBI GATC 4 cut(s) 73, 79, 981, 1238
BstMWI GCNNNNNNNGC 4 cut(s) 165, 701, 710, 869
BstNSI RCATGY 2 cut(s) 974, 1126
BstSFI CTRYAG 3 cut(s) 764, 806, 1386
BstV1I GCAGC 4 cut(s) 142, 268, 925, 1211
BsuI GTATCC 1 cut(s) 848
BsuRI GGCC 2 cut(s) 303, 1088
BtgI CCRYGG 1 cut(s) 664
BtsI GCAGTG 1 cut(s) 1009
BtsIMutI CAGTG 4 cut(s) 466, 470, 1009, 1126
BveI ACCTGC 1 cut(s) 124
Cac8I GCNNGC 5 cut(s) 736, 740, 774, 1124, 1365
CciI TCATGA 3 cut(s) 82, 118, 1081
CfoI GCGC 1 cut(s) 872
Cfr13I GGNCC 4 cut(s) 566, 908, 925, 1087
Csp6I GTAC 2 cut(s) 172, 614
CviQI GTAC 2 cut(s) 172, 614
DdeI CTNAG 6 cut(s) 100, 451, 458, 473, 678, 902
DpnI GATC 4 cut(s) 75, 81, 983, 1240
DpnII GATC 4 cut(s) 73, 79, 981, 1238
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 891, 1178
EarI CTCTTC 2 cut(s) 891, 1178
Eco130I CCWWGG 1 cut(s) 664
Eco24I GRGCYC 2 cut(s) 825, 1168
Eco47I GGWCC 3 cut(s) 566, 908, 925
Eco57I CTGAAG 1 cut(s) 915
EcoO109I RGGNCCY 2 cut(s) 925, 1087
EcoT14I CCWWGG 1 cut(s) 664
EcoT38I GRGCYC 2 cut(s) 825, 1168
ErhI CCWWGG 1 cut(s) 664
FaqI GGGAC 1 cut(s) 1232
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 1 cut(s) 865
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 4 cut(s) 131, 282, 939, 1200
FriOI GRGCYC 2 cut(s) 825, 1168
Fsp4HI GCNGC 4 cut(s) 131, 282, 939, 1200
FspBI CTAG 5 cut(s) 11, 422, 617, 746, 1311
GlaI GCGC 1 cut(s) 871
GluI GCNGC 4 cut(s) 131, 282, 939, 1200
GsuI CTGGAG 1 cut(s) 1337
HaeIII GGCC 2 cut(s) 303, 1088
HapII CCGG 1 cut(s) 501
HhaI GCGC 1 cut(s) 872
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HincII GTYRAC 2 cut(s) 759, 1405
HindII GTYRAC 2 cut(s) 759, 1405
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 5 cut(s) 122, 309, 464, 840, 1020
HpaII CCGG 1 cut(s) 501
HphI GGTGA 3 cut(s) 163, 490, 1306
Hpy166II GTNNAC 4 cut(s) 759, 1228, 1245, 1405
Hpy188I TCNGA 5 cut(s) 195, 317, 454, 883, 1163
Hpy188III TCNNGA 7 cut(s) 77, 83, 119, 385, 893, 1082, 1192
Hpy8I GTNNAC 4 cut(s) 759, 1228, 1245, 1405
HpyAV CCTTC 5 cut(s) 8, 706, 899, 1178, 1291
HpyCH4III ACNGT 3 cut(s) 381, 756, 877
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 701, 710, 869
HpyF3I CTNAG 6 cut(s) 100, 451, 458, 473, 678, 902
HspAI GCGC 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 4 cut(s) 73, 79, 981, 1238
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 1 cut(s) 483
Lsp1109I GCAGC 4 cut(s) 142, 268, 925, 1211
LweI GCATC 1 cut(s) 186
MaeI CTAG 5 cut(s) 11, 422, 617, 746, 1311
MaeIII GTNAC 6 cut(s) 17, 151, 505, 580, 778, 1192
MalI GATC 4 cut(s) 75, 81, 983, 1240
MboI GATC 4 cut(s) 73, 79, 981, 1238
MboII GAAGA 4 cut(s) 410, 908, 980, 1165
MfeI CAATTG 1 cut(s) 163
MhlI GDGCHC 3 cut(s) 436, 825, 1168
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 6 cut(s) 163, 652, 1045, 1077, 1324, 1423
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 2 cut(s) 849, 1014
MmeI TCCRAC 2 cut(s) 194, 560
Mox20I TGGCCA 1 cut(s) 303
MroXI GAANNNNTTC 2 cut(s) 888, 1040
MscI TGGCCA 1 cut(s) 303
MseI TTAA 6 cut(s) 447, 621, 921, 1107, 1290, 1413
MslI CAYNNNNRTG 2 cut(s) 503, 1292
Msp20I TGGCCA 1 cut(s) 303
MspI CCGG 1 cut(s) 501
MunI CAATTG 1 cut(s) 163
MwoI GCNNNNNNNGC 4 cut(s) 165, 701, 710, 869
NcoI CCATGG 1 cut(s) 664
NdeI CATATG 1 cut(s) 865
NdeII GATC 4 cut(s) 73, 79, 981, 1238
NlaIV GGNNCC 2 cut(s) 479, 568
NmuCI GTSAC 4 cut(s) 151, 505, 580, 1192
NspI RCATGY 2 cut(s) 974, 1126
OliI CACNNNNGTG 1 cut(s) 503
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 3 cut(s) 82, 118, 1081
PciI ACATGT 1 cut(s) 970
PciSI GCTCTTC 1 cut(s) 891
PdmI GAANNNNTTC 2 cut(s) 888, 1040
PfeI GAWTC 3 cut(s) 122, 309, 464
PkrI GCNGC 4 cut(s) 132, 283, 940, 1201
PleI GAGTC 2 cut(s) 848, 1014
PpsI GAGTC 2 cut(s) 848, 1014
PpuMI RGGWCCY 1 cut(s) 925
PscI ACATGT 1 cut(s) 970
PsiI TTATAA 1 cut(s) 1373
Psp5II RGGWCCY 1 cut(s) 925
PspN4I GGNNCC 2 cut(s) 479, 568
PspPI GGNCC 4 cut(s) 566, 908, 925, 1087
PspPPI RGGWCCY 1 cut(s) 925
PstI CTGCAG 3 cut(s) 768, 810, 1390
RsaI GTAC 2 cut(s) 173, 615
RsaNI GTAC 2 cut(s) 172, 614
RseI CAYNNNNRTG 2 cut(s) 503, 1292
SapI GCTCTTC 1 cut(s) 891
SaqAI TTAA 6 cut(s) 447, 621, 921, 1107, 1290, 1413
SatI GCNGC 4 cut(s) 131, 282, 939, 1200
Sau3AI GATC 4 cut(s) 73, 79, 981, 1238
Sau96I GGNCC 4 cut(s) 566, 908, 925, 1087
SchI GAGTC 2 cut(s) 849, 1014
SduI GDGCHC 3 cut(s) 436, 825, 1168
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 3 cut(s) 764, 806, 1386
SinI GGWCC 3 cut(s) 566, 908, 925
SmiMI CAYNNNNRTG 2 cut(s) 503, 1292
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SpeI ACTAGT 1 cut(s) 616
SphI GCATGC 1 cut(s) 1126
Sse9I AATT 6 cut(s) 163, 652, 1045, 1077, 1324, 1423
SsiI CCGC 4 cut(s) 168, 258, 732, 1147
SspMI CTAG 5 cut(s) 11, 422, 617, 746, 1311
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 3 cut(s) 381, 756, 877
TaqI TCGA 3 cut(s) 61, 386, 1397
TasI AATT 6 cut(s) 163, 652, 1045, 1077, 1324, 1423
TfiI GAWTC 3 cut(s) 122, 309, 464
Tru1I TTAA 6 cut(s) 447, 621, 921, 1107, 1290, 1413
Tru9I TTAA 6 cut(s) 447, 621, 921, 1107, 1290, 1413
TscAI CASTG 4 cut(s) 466, 477, 1016, 1133
TseFI GTSAC 4 cut(s) 151, 505, 580, 1192
TseI GCWGC 4 cut(s) 130, 281, 938, 1199
Tsp45I GTSAC 4 cut(s) 151, 505, 580, 1192
TspRI CASTG 4 cut(s) 466, 477, 1016, 1133
VpaK11BI GGWCC 3 cut(s) 566, 908, 925
XapI RAATTY 1 cut(s) 1324
XceI RCATGY 2 cut(s) 974, 1126
XmnI GAANNNNTTC 2 cut(s) 888, 1040
XspI CTAG 5 cut(s) 11, 422, 617, 746, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.