Rh7BG124500

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0011559
Physical Location & Seq
Forward (+)
9268221 .. 9270460
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG124500.1

Sequence Viewer

Length: 1095 bp
ATGCTAACTGGCTTTAATTTGGCTGCTTTTCACTTCATCAATGGCCAAAGATTCATCAGATACAGAGATATTATGGGCTATTTATATGGCAGAGAAATGTATTACATAACATGGGTTTTACAGTTCTCGACCCTACTTCTTGGAAATATGGGTTTCATTCTTCTAGGGGGTAGAGCACTCAAGGAGATTAACTCAGAACTCAGTGATTCTCCACTGAGGCTCCAATACTTTATTGTCATCACCGGAGTGACCTACTTCATATTCGCCTTTTTCATTCCAACAATGTCTAAAATGAGATTTTGGCTTGGACCCTCTACCATTATCACTTTTGCTTACATTGTCATACTCTTGGTGGTACTAGTTAAAGATGGGAAAACAAAGACGACAGAGCGAAATTATGAAATCCATGGAAACAAAGCTGAGAGAGTGTTCAATGCCTTCAATGCAGTTTCAGCTATTATTGTCTGCAACACCTCAGGCTTGCTACTAGAAATACAGTCAACCCTACAGAAGCCAGCAGTCACAAATATGAGGAAAGCTTTATATCTGCAGTATAGTGCAGGGCTCTTATTTTACTATGGAGTCAGCATAGTAGGATACTGGGCATATGGCGCAACCGTATCTGAATACCTTCCTGAAGAGCTAAGTGGTCCTAAATCGGTTAAGGTCCTCATAAACGCTGCCGTGTTTCTACAATCCATAGTCTCACAACATATGTTTGTTGCACCAATTCATGAGGCCCTTGATACCAAGTTCCTTAAACTTGATGAGAGCATGCACTGGAAATATAACTTCAAGCGGAGGTTCTACCTCCGAGCCCTTCTCTTCATGGGAAATACATTTGTGACAGCAGCTATTCCTTTCATGGGGGACTTTGTGAACTTATTTGGATCGTTTACACTCGTTCCTCTAACTTTTGTTTTTCCAAGCATGATTTTCATTAAGGTAAAGGGAAAGACAGCTAGACTGGAGAAAAATTTGTGGCATTGGTTCAACATTGTTATTTTTTCTCTGCTTGCGATTATAACTACAATTTCTGCAGTTCGTTCGATTGTCAACAATGTTAAGGAATACAATTTCTTTGCAGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

364

Amino Acids

41.71

Weight (kDa)

9.62

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 14 - 347 4.6e-51 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1025
AciI CCGC 1 cut(s) 799
AclWI GGATC 1 cut(s) 898
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 1 cut(s) 976
AcuI CTGAAG 1 cut(s) 657
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 866
AgsI TTSAA 4 cut(s) 433, 442, 796, 994
AhlI ACTAGT 1 cut(s) 358
AleI CACNNNNGTG 1 cut(s) 245
AluBI AGCT 6 cut(s) 419, 455, 539, 643, 854, 962
AluI AGCT 6 cut(s) 419, 455, 539, 643, 854, 962
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 709
AlwI GGATC 1 cut(s) 898
AoxI GGCC 2 cut(s) 43, 738
ApeKI GCWGC 3 cut(s) 23, 680, 851
ApoI RAATTY 1 cut(s) 976
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 4 cut(s) 308, 650, 667, 739
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 3 cut(s) 308, 650, 667
AxyI CCTNAGG 1 cut(s) 475
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 2 cut(s) 567, 820
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 10, 667, 863
BccI CCATC 1 cut(s) 362
BceAI ACGGC 1 cut(s) 668
BcgI CGANNNNNNTGC 2 cut(s) 1029, 1063
BciVI GTATCC 1 cut(s) 590
BcoDI GTCTC 1 cut(s) 709
BcuI ACTAGT 1 cut(s) 358
BfaI CTAG 4 cut(s) 164, 359, 488, 963
BfmI CTRYAG 3 cut(s) 506, 548, 1038
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 3 cut(s) 24, 681, 852
BlsI GCNGC 3 cut(s) 25, 682, 853
Bme18I GGWCC 3 cut(s) 308, 650, 667
BmgT120I GGNCC 4 cut(s) 308, 650, 667, 739
BmiI GGNNCC 2 cut(s) 221, 310
BmrI ACTGGG 1 cut(s) 610
BmuI ACTGGG 1 cut(s) 610
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 807, 839
BpmI CTGGAG 1 cut(s) 989
BpuEI CTTGAG 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 406
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 866
Bse1I ACTGG 4 cut(s) 13, 605, 785, 972
Bse21I CCTNAGG 1 cut(s) 475
BseDI CCNNGG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 866
BseMII CTCAG 5 cut(s) 206, 207, 214, 411, 489
BseNI ACTGG 4 cut(s) 13, 605, 785, 972
BseXI GCAGC 3 cut(s) 10, 667, 863
BsgI GTGCAG 1 cut(s) 579
BshFI GGCC 2 cut(s) 45, 740
BsiHKAI GWGCWC 1 cut(s) 178
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 1 cut(s) 866
BsmAI GTCTC 1 cut(s) 709
BsmFI GGGAC 1 cut(s) 884
BsnI GGCC 2 cut(s) 45, 740
Bsp1286I GDGCHC 3 cut(s) 178, 567, 820
Bsp143I GATC 1 cut(s) 890
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 1 cut(s) 799
BspANI GGCC 2 cut(s) 45, 740
BspCNI CTCAG 5 cut(s) 206, 207, 213, 412, 488
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 2 cut(s) 221, 310
BspMAI CTGCAG 2 cut(s) 552, 1042
BspPI GGATC 1 cut(s) 898
BspQI GCTCTTC 1 cut(s) 633
BsrI ACTGG 4 cut(s) 13, 605, 785, 972
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 1 cut(s) 890
BssT1I CCWWGG 1 cut(s) 406
Bst4CI ACNGT 3 cut(s) 123, 498, 619
Bst6I CTCTTC 2 cut(s) 633, 830
BstC8I GCNNGC 4 cut(s) 482, 516, 776, 1017
BstDEI CTNAG 6 cut(s) 193, 200, 215, 420, 475, 644
BstDSI CCRYGG 1 cut(s) 406
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 1 cut(s) 893
BstMAI GTCTC 1 cut(s) 709
BstMBI GATC 1 cut(s) 890
BstMWI GCNNNNNNNGC 3 cut(s) 443, 452, 611
BstNSI RCATGY 1 cut(s) 778
BstSFI CTRYAG 3 cut(s) 506, 548, 1038
BstV1I GCAGC 3 cut(s) 10, 667, 863
Bsu36I CCTNAGG 1 cut(s) 475
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 45, 740
BtgI CCRYGG 1 cut(s) 406
BtsIMutI CAGTG 3 cut(s) 208, 212, 778
Cac8I GCNNGC 4 cut(s) 482, 516, 776, 1017
CciI TCATGA 1 cut(s) 733
CfoI GCGC 1 cut(s) 614
Cfr13I GGNCC 4 cut(s) 308, 650, 667, 739
Csp6I GTAC 1 cut(s) 356
CviAII CATG 8 cut(s) 111, 407, 734, 775, 829, 865, 931, 1092
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 6 cut(s) 193, 200, 215, 420, 475, 644
DpnI GATC 1 cut(s) 892
DpnII GATC 1 cut(s) 890
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 2 cut(s) 633, 830
EarI CTCTTC 2 cut(s) 633, 830
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 2 cut(s) 567, 820
Eco47I GGWCC 3 cut(s) 308, 650, 667
Eco57I CTGAAG 1 cut(s) 657
Eco81I CCTNAGG 1 cut(s) 475
EcoO109I RGGNCCY 2 cut(s) 667, 739
EcoT14I CCWWGG 1 cut(s) 406
EcoT38I GRGCYC 2 cut(s) 567, 820
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 8 cut(s) 114, 410, 737, 778, 832, 868, 934, 1095
FaqI GGGAC 1 cut(s) 884
FatI CATG 8 cut(s) 110, 406, 733, 774, 828, 864, 930, 1091
FauNDI CATATG 2 cut(s) 607, 714
Fnu4HI GCNGC 3 cut(s) 24, 681, 852
FriOI GRGCYC 2 cut(s) 567, 820
Fsp4HI GCNGC 3 cut(s) 24, 681, 852
FspBI CTAG 4 cut(s) 164, 359, 488, 963
GlaI GCGC 1 cut(s) 613
GluI GCNGC 3 cut(s) 24, 681, 852
GsuI CTGGAG 1 cut(s) 989
HaeIII GGCC 2 cut(s) 45, 740
HapII CCGG 1 cut(s) 243
HhaI GCGC 1 cut(s) 614
Hin1II CATG 8 cut(s) 114, 410, 737, 778, 832, 868, 934, 1095
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HincII GTYRAC 2 cut(s) 501, 1057
HindII GTYRAC 2 cut(s) 501, 1057
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 3 cut(s) 51, 206, 582
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 4 cut(s) 501, 880, 897, 1057
Hpy188I TCNGA 4 cut(s) 59, 196, 625, 815
Hpy188III TCNNGA 3 cut(s) 127, 635, 734
Hpy8I GTNNAC 4 cut(s) 501, 880, 897, 1057
HpyAV CCTTC 3 cut(s) 448, 641, 830
HpyCH4III ACNGT 3 cut(s) 123, 498, 619
HpyCH4V TGCA 8 cut(s) 446, 468, 550, 560, 725, 778, 1040, 1085
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 452, 611
HpyF3I CTNAG 6 cut(s) 193, 200, 215, 420, 475, 644
Hsp92II CATG 8 cut(s) 114, 410, 737, 778, 832, 868, 934, 1095
HspAI GCGC 1 cut(s) 612
Kzo9I GATC 1 cut(s) 890
LguI GCTCTTC 1 cut(s) 633
LmnI GCTCC 1 cut(s) 225
LpnPI CCDG 8 cut(s) 256, 462, 528, 546, 586, 648, 766, 953
Lsp1109I GCAGC 3 cut(s) 10, 667, 863
MaeI CTAG 4 cut(s) 164, 359, 488, 963
MaeIII GTNAC 3 cut(s) 247, 520, 844
MalI GATC 1 cut(s) 892
MboI GATC 1 cut(s) 890
MboII GAAGA 3 cut(s) 152, 650, 817
MhlI GDGCHC 3 cut(s) 178, 567, 820
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 6 cut(s) 16, 394, 729, 976, 1032, 1075
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 1 cut(s) 591
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 9 cut(s) 210, 322, 484, 525, 680, 730, 795, 821, 918
Mox20I TGGCCA 1 cut(s) 45
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 1 cut(s) 45
MseI TTAA 7 cut(s) 15, 189, 363, 663, 759, 942, 1065
MslI CAYNNNNRTG 2 cut(s) 245, 527
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 1 cut(s) 243
MwoI GCNNNNNNNGC 3 cut(s) 443, 452, 611
NcoI CCATGG 1 cut(s) 406
NdeI CATATG 2 cut(s) 607, 714
NdeII GATC 1 cut(s) 890
NlaIII CATG 8 cut(s) 114, 410, 737, 778, 832, 868, 934, 1095
NlaIV GGNNCC 2 cut(s) 221, 310
NmuCI GTSAC 3 cut(s) 247, 520, 844
NspI RCATGY 1 cut(s) 778
OliI CACNNNNGTG 1 cut(s) 245
PaeI GCATGC 1 cut(s) 778
PagI TCATGA 1 cut(s) 733
PciSI GCTCTTC 1 cut(s) 633
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 51, 206
PkrI GCNGC 3 cut(s) 25, 682, 853
PleI GAGTC 1 cut(s) 590
PpsI GAGTC 1 cut(s) 590
PpuMI RGGWCCY 1 cut(s) 667
PsiI TTATAA 1 cut(s) 1025
Psp5II RGGWCCY 1 cut(s) 667
PspN4I GGNNCC 2 cut(s) 221, 310
PspPI GGNCC 4 cut(s) 308, 650, 667, 739
PspPPI RGGWCCY 1 cut(s) 667
PstI CTGCAG 2 cut(s) 552, 1042
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 2 cut(s) 245, 527
SapI GCTCTTC 1 cut(s) 633
SaqAI TTAA 7 cut(s) 15, 189, 363, 663, 759, 942, 1065
SatI GCNGC 3 cut(s) 24, 681, 852
Sau3AI GATC 1 cut(s) 890
Sau96I GGNCC 4 cut(s) 308, 650, 667, 739
SchI GAGTC 1 cut(s) 591
SduI GDGCHC 3 cut(s) 178, 567, 820
SfcI CTRYAG 3 cut(s) 506, 548, 1038
SinI GGWCC 3 cut(s) 308, 650, 667
SmiMI CAYNNNNRTG 2 cut(s) 245, 527
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SpeI ACTAGT 1 cut(s) 358
SphI GCATGC 1 cut(s) 778
Sse9I AATT 6 cut(s) 16, 394, 729, 976, 1032, 1075
SsiI CCGC 1 cut(s) 799
SspMI CTAG 4 cut(s) 164, 359, 488, 963
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 3 cut(s) 123, 498, 619
TaqI TCGA 2 cut(s) 128, 1049
TasI AATT 6 cut(s) 16, 394, 729, 976, 1032, 1075
TfiI GAWTC 2 cut(s) 51, 206
Tru1I TTAA 7 cut(s) 15, 189, 363, 663, 759, 942, 1065
Tru9I TTAA 7 cut(s) 15, 189, 363, 663, 759, 942, 1065
TscAI CASTG 3 cut(s) 208, 219, 785
TseFI GTSAC 3 cut(s) 247, 520, 844
TseI GCWGC 3 cut(s) 23, 680, 851
Tsp45I GTSAC 3 cut(s) 247, 520, 844
TspRI CASTG 3 cut(s) 208, 219, 785
VpaK11BI GGWCC 3 cut(s) 308, 650, 667
XapI RAATTY 1 cut(s) 976
XceI RCATGY 1 cut(s) 778
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 4 cut(s) 164, 359, 488, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.