MD06G1162100.v1.1

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011559
Physical Location & Seq
Forward (+)
30282963 .. 30287575
4613 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1162100.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGGAAGGAGAAGAAGGTAACACAAGAAACACCAAAGAAGCTCTGAGCACTGAAGGAGGCCATATAAAGGGTGACCAAGATCGTCAATATGCCCTCCCATCTGCTCATACAATTGGCCACGATTCATGGCAACAAGTTGGGGTGATGCTGGTGACAGCCTTCAACTGTGGATATATATTGAGCTTTTCAAATCTTATGTTGGTGCCTTTGGGATGGACATGGGGGATCATATGCCTTCTGGTTTTTGGGCTCTACAGTGCCTATGCAATCCGGCTTTTGGCTGCTTTTCACTTCATCGATGGCCAAAGGTTCATCAGATACAGAGATCTCATGGGTTTTTTATTTGGCAGAGAGATGTTTTACACCACTTGGGTTTTCCAGTTTTTGACCCTTCTACTTGGAAACATGGGTTTCATTCTACTTGGGGGAAGAGCACTTAAGGAAATTAACTCCGAATTCAGCGACTCACCATTTCGGCTTCAGTATTTTATCGCCATCACCGGAGTGACCTACTTTGTATTTGCATTCTTCATTCCAACAATGTCTGCAATGAGAAGTTGGCTCGGACCATCTACCGTTATCACCTTCGCTTACATTGTCATGCTCTTGGTGGTGGTGGTTAAAGATGGGAAAGCTAACACCAAGAGAGATTATGCGATCCATGGAAACGAAGCAGACAGAGTACTCAATGCCTTTGGTGCAATTGCAGCTATTATCGTATGCAACTCCTCTGGCTTGCTACTCGAAATACAGTCAACTCTAAGAGATCCTGCAGTAACAAATATGAGGAAAGCTTTAAATCTGCAGTTTACTGTGGGGCTGGTGCTTTACTATGGAGTGAGCATAGTCGGATACTGGGCTTATGGCTCAACTGTATCCGAATACCTTCCTCAAGAGCTAAGCGGTCCTAAATCGGTTAAGGTTCTCATAAACGCCACTGTTTTTCTACAATCCGTGGTCTCCCAACATATGTTTGTGGCACCAATCCACGAGTTCCTTGATACCAAGTTCCTCAAACTCGATAAGAGCATGAACTCGAAAGGAAATTTGAAGCGCAGATTGTACGTTCGAGCCCTCCTCTTTGCAGTAAATACATTTGTAACAGCAGCTTTTCCTTTCATGGGGGACTTCGTAAACTTGTTTGGATCCTTTACACTCGTTCCTCTAACTTTCGTGTTCCCGAGCATGATTTTCATCAAGGTGAAGGGAAAGGCAGCTTCACCGGAGAAGATTTTATGGCATTGGTTCAACATCGTTATTTTTTCGCTCCTTGCCGTCGTCACCACAATTTCTGCCGTTCGATTGATAGTCAACAATGTTAAGGAATACAATTTCTTTGCAAATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

450

Amino Acids

50.4

Weight (kDa)

9.3

Isoelectric Point (pI)

28.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 41 - 433 6.1e-60 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 202, 979
AciI CCGC 1 cut(s) 903
AclWI GGATC 5 cut(s) 233, 652, 761, 1140, 1153
AcoI YGGCCR 2 cut(s) 115, 301
AcsI RAATTY 2 cut(s) 455, 1045
AcuI CTGAAG 2 cut(s) 72, 464
AfaI GTAC 2 cut(s) 684, 1064
AfiI CCNNNNNNNGG 3 cut(s) 67, 277, 1121
AflII CTTAAG 1 cut(s) 437
AgsI TTSAA 4 cut(s) 163, 189, 1051, 1249
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 8 cut(s) 41, 183, 635, 710, 794, 898, 1109, 1217
AluI AGCT 8 cut(s) 41, 183, 635, 710, 794, 898, 1109, 1217
Alw21I GWGCWC 2 cut(s) 50, 436
Alw26I GTCTC 1 cut(s) 964
AlwI GGATC 5 cut(s) 233, 652, 761, 1140, 1153
Ama87I CYCGRG 1 cut(s) 1180
AoxI GGCC 3 cut(s) 58, 115, 301
ApeKI GCWGC 4 cut(s) 281, 707, 1106, 1214
ApoI RAATTY 2 cut(s) 455, 1045
Asp700I GAANNNNTTC 1 cut(s) 885
AspLEI GCGC 1 cut(s) 1056
AspS9I GGNCC 2 cut(s) 566, 905
AsuHPI GGTGA 9 cut(s) 83, 154, 163, 459, 490, 574, 1212, 1213, 1273
AvaI CYCGRG 1 cut(s) 1180
AvaII GGWCC 2 cut(s) 566, 905
BalI TGGCCA 2 cut(s) 117, 303
BamHI GGATCC 1 cut(s) 1145
BanI GGYRCC 2 cut(s) 202, 979
BanII GRGCYC 2 cut(s) 252, 1075
BauI CACGAG 1 cut(s) 989
Bbv12I GWGCWC 2 cut(s) 50, 436
BbvI GCAGC 4 cut(s) 268, 719, 1118, 1226
BccI CCATC 6 cut(s) 106, 207, 293, 503, 577, 620
BceAI ACGGC 2 cut(s) 1259, 1280
BciVI GTATCC 2 cut(s) 845, 886
BcoDI GTCTC 1 cut(s) 964
BfmI CTRYAG 3 cut(s) 253, 771, 803
BfrI CTTAAG 1 cut(s) 437
BfuI GTATCC 2 cut(s) 845, 886
BglII AGATCT 1 cut(s) 325
BisI GCNGC 4 cut(s) 282, 708, 1107, 1215
BlpI GCTNAGC 1 cut(s) 899
BlsI GCNGC 4 cut(s) 283, 709, 1108, 1216
BmcAI AGTACT 1 cut(s) 684
Bme18I GGWCC 2 cut(s) 566, 905
BmeT110I CYCGRG 1 cut(s) 1180
BmgT120I GGNCC 2 cut(s) 566, 905
BmiI GGNNCC 3 cut(s) 204, 981, 1147
BmrI ACTGGG 1 cut(s) 865
BmsI GCATC 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 865
BplI GAGNNNNNCTC 2 cut(s) 1062, 1094
Bpu1102I GCTNAGC 1 cut(s) 899
BpuEI CTTGAG 1 cut(s) 876
Bsa29I ATCGAT 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 1193
BsaI GGTCTC 1 cut(s) 964
BsaJI CCNNGG 2 cut(s) 661, 954
BsaWI WCCGGW 2 cut(s) 500, 1222
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 277, 1121
Bse1I ACTGG 2 cut(s) 379, 860
Bse3DI GCAATG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 1193
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 661, 954
BseGI GGATG 1 cut(s) 218
BseJI GATNNNNATC 1 cut(s) 1193
BseLI CCNNNNNNNGG 3 cut(s) 67, 277, 1121
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 2 cut(s) 379, 860
BseRI GAGGAG 2 cut(s) 718, 1067
BseXI GCAGC 4 cut(s) 268, 719, 1118, 1226
BshFI GGCC 3 cut(s) 60, 117, 303
BshNI GGYRCC 2 cut(s) 202, 979
BshVI ATCGAT 1 cut(s) 297
BsiHKAI GWGCWC 2 cut(s) 50, 436
BsiHKCI CYCGRG 1 cut(s) 1180
BsiSI CCGG 3 cut(s) 271, 501, 1223
BslFI GGGAC 1 cut(s) 1139
BslI CCNNNNNNNGG 3 cut(s) 67, 277, 1121
BsmAI GTCTC 1 cut(s) 964
BsmFI GGGAC 1 cut(s) 1139
BsmI GAATGC 1 cut(s) 524
BsnI GGCC 3 cut(s) 60, 117, 303
Bso31I GGTCTC 1 cut(s) 964
BsoBI CYCGRG 1 cut(s) 1180
Bsp1286I GDGCHC 4 cut(s) 50, 252, 436, 1075
Bsp143I GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
Bsp1720I GCTNAGC 1 cut(s) 899
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 1 cut(s) 903
BspANI GGCC 3 cut(s) 60, 117, 303
BspCNI CTCAG 1 cut(s) 36
BspDI ATCGAT 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 204, 981, 1147
BspMAI CTGCAG 2 cut(s) 775, 807
BspPI GGATC 5 cut(s) 233, 652, 761, 1140, 1153
BspQI GCTCTTC 1 cut(s) 424
BspT107I GGYRCC 2 cut(s) 202, 979
BspTI CTTAAG 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 964
BsrDI GCAATG 1 cut(s) 555
BsrI ACTGG 2 cut(s) 379, 860
BssECI CCNNGG 2 cut(s) 661, 954
BssMI GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
BssSI CACGAG 1 cut(s) 989
BssT1I CCWWGG 1 cut(s) 661
Bst2BI CACGAG 1 cut(s) 989
Bst4CI ACNGT 7 cut(s) 167, 257, 577, 753, 814, 874, 940
Bst6I CTCTTC 1 cut(s) 424
BstAFI CTTAAG 1 cut(s) 437
BstC8I GCNNGC 1 cut(s) 737
BstDEI CTNAG 3 cut(s) 44, 761, 899
BstDSI CCRYGG 2 cut(s) 661, 954
BstEII GGTNACC 1 cut(s) 71
BstF5I GGATG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 1056
BstKTI GATC 6 cut(s) 82, 228, 328, 660, 769, 1148
BstMAI GTCTC 1 cut(s) 964
BstMBI GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
BstMWI GCNNNNNNNGC 2 cut(s) 698, 707
BstPI GGTNACC 1 cut(s) 71
BstSFI CTRYAG 3 cut(s) 253, 771, 803
BstV1I GCAGC 4 cut(s) 268, 719, 1118, 1226
BstX2I RGATCY 3 cut(s) 325, 766, 1145
BstYI RGATCY 3 cut(s) 325, 766, 1145
Bsu15I ATCGAT 1 cut(s) 297
BsuI GTATCC 2 cut(s) 845, 886
BsuRI GGCC 3 cut(s) 60, 117, 303
BsuTUI ATCGAT 1 cut(s) 297
BtgI CCRYGG 2 cut(s) 661, 954
BtsCI GGATG 1 cut(s) 218
BtsIMutI CAGTG 3 cut(s) 48, 262, 936
Cac8I GCNNGC 1 cut(s) 737
CfoI GCGC 1 cut(s) 1056
Cfr13I GGNCC 2 cut(s) 566, 905
ClaI ATCGAT 1 cut(s) 297
Csp6I GTAC 2 cut(s) 683, 1063
CviQI GTAC 2 cut(s) 683, 1063
DdeI CTNAG 3 cut(s) 44, 761, 899
DpnI GATC 6 cut(s) 81, 227, 327, 659, 768, 1147
DpnII GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
DraI TTTAAA 1 cut(s) 798
EaeI YGGCCR 2 cut(s) 115, 301
Eam1104I CTCTTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 424
Eco130I CCWWGG 1 cut(s) 661
Eco24I GRGCYC 2 cut(s) 252, 1075
Eco31I GGTCTC 1 cut(s) 964
Eco47I GGWCC 2 cut(s) 566, 905
Eco57I CTGAAG 2 cut(s) 72, 464
Eco88I CYCGRG 1 cut(s) 1180
Eco91I GGTNACC 1 cut(s) 71
EcoO65I GGTNACC 1 cut(s) 71
EcoRI GAATTC 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 661
EcoT38I GRGCYC 2 cut(s) 252, 1075
ErhI CCWWGG 1 cut(s) 661
FaqI GGGAC 1 cut(s) 1139
FauNDI CATATG 2 cut(s) 230, 969
Fnu4HI GCNGC 4 cut(s) 282, 708, 1107, 1215
FokI GGATG 1 cut(s) 225
FriOI GRGCYC 2 cut(s) 252, 1075
Fsp4HI GCNGC 4 cut(s) 282, 708, 1107, 1215
GlaI GCGC 1 cut(s) 1055
GluI GCNGC 4 cut(s) 282, 708, 1107, 1215
HaeIII GGCC 3 cut(s) 60, 117, 303
HapII CCGG 3 cut(s) 271, 501, 1223
HhaI GCGC 1 cut(s) 1056
Hin6I GCGC 1 cut(s) 1054
HinP1I GCGC 1 cut(s) 1054
HincII GTYRAC 2 cut(s) 756, 1312
HindII GTYRAC 2 cut(s) 756, 1312
HindIII AAGCTT 1 cut(s) 792
HinfI GANTC 2 cut(s) 122, 464
HpaII CCGG 3 cut(s) 271, 501, 1223
HphI GGTGA 9 cut(s) 83, 154, 163, 459, 490, 574, 1212, 1213, 1273
Hpy166II GTNNAC 4 cut(s) 756, 810, 1135, 1312
Hpy188I TCNGA 6 cut(s) 45, 317, 454, 566, 851, 880
Hpy188III TCNNGA 2 cut(s) 893, 1180
Hpy8I GTNNAC 4 cut(s) 756, 810, 1135, 1312
Hpy99I CGWCG 1 cut(s) 1280
HpyAV CCTTC 8 cut(s) 8, 47, 169, 245, 401, 595, 896, 1198
HpyCH4III ACNGT 7 cut(s) 167, 257, 577, 753, 814, 874, 940
HpyCH4IV ACGT 1 cut(s) 1065
HpyF10VI GCNNNNNNNGC 2 cut(s) 698, 707
HpyF3I CTNAG 3 cut(s) 44, 761, 899
HpySE526I ACGT 1 cut(s) 1065
HspAI GCGC 1 cut(s) 1054
Kzo9I GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
LguI GCTCTTC 1 cut(s) 424
LmnI GCTCC 1 cut(s) 1272
Lsp1109I GCAGC 4 cut(s) 268, 719, 1118, 1226
LweI GCATC 1 cut(s) 135
MaeII ACGT 1 cut(s) 1065
MaeIII GTNAC 7 cut(s) 17, 71, 151, 505, 775, 1099, 1279
MalI GATC 6 cut(s) 81, 227, 327, 659, 768, 1147
MboI GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
MboII GAAGA 4 cut(s) 23, 441, 520, 1240
MfeI CAATTG 2 cut(s) 111, 702
MflI RGATCY 3 cut(s) 325, 766, 1145
MhlI GDGCHC 4 cut(s) 50, 252, 436, 1075
MlsI TGGCCA 2 cut(s) 117, 303
MluCI AATT 7 cut(s) 111, 444, 455, 702, 1045, 1287, 1330
MluNI TGGCCA 2 cut(s) 117, 303
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 2 cut(s) 560, 829
MnlI CCTC 9 cut(s) 50, 104, 739, 780, 900, 1022, 1085, 1088, 1173
Mox20I TGGCCA 2 cut(s) 117, 303
MroXI GAANNNNTTC 1 cut(s) 885
MscI TGGCCA 2 cut(s) 117, 303
MseI TTAA 6 cut(s) 438, 447, 621, 797, 918, 1320
MslI CAYNNNNRTG 3 cut(s) 503, 599, 1199
Msp20I TGGCCA 2 cut(s) 117, 303
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 3 cut(s) 271, 501, 1223
MunI CAATTG 2 cut(s) 111, 702
Mva1269I GAATGC 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 698, 707
NcoI CCATGG 1 cut(s) 661
NdeI CATATG 2 cut(s) 230, 969
NdeII GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
NlaIV GGNNCC 3 cut(s) 204, 981, 1147
NmuCI GTSAC 4 cut(s) 71, 151, 505, 1279
OliI CACNNNNGTG 1 cut(s) 503
PciSI GCTCTTC 1 cut(s) 424
PctI GAATGC 1 cut(s) 524
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 1 cut(s) 122
PkrI GCNGC 4 cut(s) 283, 709, 1108, 1216
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
PspEI GGTNACC 1 cut(s) 71
PspN4I GGNNCC 3 cut(s) 204, 981, 1147
PspPI GGNCC 2 cut(s) 566, 905
PstI CTGCAG 2 cut(s) 775, 807
PsuI RGATCY 3 cut(s) 325, 766, 1145
RsaI GTAC 2 cut(s) 684, 1064
RsaNI GTAC 2 cut(s) 683, 1063
RseI CAYNNNNRTG 3 cut(s) 503, 599, 1199
SapI GCTCTTC 1 cut(s) 424
SaqAI TTAA 6 cut(s) 438, 447, 621, 797, 918, 1320
SatI GCNGC 4 cut(s) 282, 708, 1107, 1215
Sau3AI GATC 6 cut(s) 79, 225, 325, 657, 766, 1145
Sau96I GGNCC 2 cut(s) 566, 905
ScaI AGTACT 1 cut(s) 684
SchI GAGTC 1 cut(s) 458
SduI GDGCHC 4 cut(s) 50, 252, 436, 1075
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 3 cut(s) 253, 771, 803
SinI GGWCC 2 cut(s) 566, 905
SmiMI CAYNNNNRTG 3 cut(s) 503, 599, 1199
SmlI CTYRAG 2 cut(s) 437, 891
SmoI CTYRAG 2 cut(s) 437, 891
Sse9I AATT 7 cut(s) 111, 444, 455, 702, 1045, 1287, 1330
SsiI CCGC 1 cut(s) 903
StyI CCWWGG 1 cut(s) 661
TaaI ACNGT 7 cut(s) 167, 257, 577, 753, 814, 874, 940
TaiI ACGT 1 cut(s) 1068
TaqI TCGA 6 cut(s) 297, 744, 1020, 1037, 1069, 1300
TasI AATT 7 cut(s) 111, 444, 455, 702, 1045, 1287, 1330
TatI WGTACW 1 cut(s) 682
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 6 cut(s) 438, 447, 621, 797, 918, 1320
Tru9I TTAA 6 cut(s) 438, 447, 621, 797, 918, 1320
TscAI CASTG 3 cut(s) 55, 262, 943
TseFI GTSAC 4 cut(s) 71, 151, 505, 1279
TseI GCWGC 4 cut(s) 281, 707, 1106, 1214
Tsp45I GTSAC 4 cut(s) 71, 151, 505, 1279
TspDTI ATGAA 8 cut(s) 114, 283, 301, 403, 520, 1046, 1108, 1183
TspGWI ACGGA 1 cut(s) 943
TspRI CASTG 3 cut(s) 55, 262, 943
Vha464I CTTAAG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 566, 905
XapI RAATTY 2 cut(s) 455, 1045
XmnI GAANNNNTTC 1 cut(s) 885
ZrmI AGTACT 1 cut(s) 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.