Rroxscaffold_3G00263350

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011559
Physical Location & Seq
Reverse (-)
57298681 .. 57300916
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00263350.1

Sequence Viewer

Length: 1209 bp
ATGATGGTGACTGGCTTCAATTGCGGGTACATATTGAGCTTTTCAAATCTGATGCTTGTGCCTTTGGGTTGGACATGGGGTATCATCTGCCTCGTTGTTGTTGGCTTTTATACTGCCTATGCTAACTGGCTTTTGGCTGCTTTTCACTTCATCAATGGCCAAAGATTCATCAGATACAGAGATATTATGGGCTATTTATATGGCAGAGAAATGTATTACATAACATGGGTTTTACAGTTCTCGACCCTACTTCTTGGAAATATGGGTTTCATTCTTCTAGGGGGTAGAGCACTCAAGGAGATTAACTCAGAACTCAGTGATTCTCCACTTAGGCTCCAATACTTTATTGTCATCACCGGAGTGACCTACTTCATATTCGCCTTTTTCATTCCAACAATGTCTAAAATGAGATTTTGGCTTGGACCCTCTACCATTGTCACTTTTGCTTACATTGTCATACTCTTGGTGGTACTAGTTAAAGATGGGAAAACAAAGACGACAGAGCGCAATTATGAAATCCATGGAAACAAAGCTGAGAGAGTGTTCAATGCCTTCAATGCAGTTTCAGCTATTATTGTCTGCAACACCGCAGGCTTGCTACTAGAAATACAGTCAACCCTGCAGAAGCCAGCAGTAACAAAAATGAGGAAAGCTTTATATCTGCAGTATAGTGCAGGGCTCTTATTTTACTATGGAGTCAGCATAGTAGGATACTGGGCATATGGCTCAACCGTATCTGAATACCTTCCTGAAGAGCTAAGTGGTCCTAAATCGGTTAAGGTCCTCATAAACGCTGCCGTGTTTCTACAATCCGTAGTCTCACAACATATGTTTGTTGCACCAATTCATGAGGCCCTTGATACCAAGTTCCTTAAACTTGATGAGAGCATGCACTGGAAATATAACTTCAAGCGGAGGTTCTACCTCCGAGCCCTTCTTTTCATGGGAAATACATTTGTGACAGCAGCTATTCCTTTCATGGGGGACTTTGTGAACTTATTTGGATCGTTAACACTCGTTCCTCTAACTTTTGTTTTTCCAAGCATGATTTTCATTAAGGTAAAGGGAAAGACAGCTAGACTGGAGAAATATTTGTGGCATTGGTTCAACATTGTTATTTTTTCTCTGCTTGCGATTATAACTACGATTTCTGCAGTTCGTTCGATTGTCAACAATGTTAAGGAATACAATTTCTTTGCAGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

402

Amino Acids

45.99

Weight (kDa)

9.52

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 10 - 385 1e-57 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1139
AciI CCGC 3 cut(s) 24, 588, 913
AclWI GGATC 1 cut(s) 1012
AcoI YGGCCR 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 2 cut(s) 29, 471
AfiI CCNNNNNNNGG 1 cut(s) 980
AgsI TTSAA 6 cut(s) 19, 45, 547, 556, 910, 1108
AhlI ACTAGT 1 cut(s) 472
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 7 cut(s) 39, 533, 569, 653, 757, 968, 1076
AluI AGCT 7 cut(s) 39, 533, 569, 653, 757, 968, 1076
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 1 cut(s) 823
AlwI GGATC 1 cut(s) 1012
AoxI GGCC 2 cut(s) 157, 852
ApeKI GCWGC 3 cut(s) 137, 794, 965
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 4 cut(s) 422, 764, 781, 853
AsuHPI GGTGA 2 cut(s) 19, 346
AvaII GGWCC 3 cut(s) 422, 764, 781
BalI TGGCCA 1 cut(s) 159
BanII GRGCYC 2 cut(s) 681, 934
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 3 cut(s) 124, 781, 977
BccI CCATC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 782
BcgI CGANNNNNNTGC 2 cut(s) 1143, 1177
BciVI GTATCC 1 cut(s) 704
BcoDI GTCTC 1 cut(s) 823
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 4 cut(s) 278, 473, 602, 1077
BfmI CTRYAG 3 cut(s) 620, 662, 1152
BfuI GTATCC 1 cut(s) 704
BisI GCNGC 3 cut(s) 138, 795, 966
BlsI GCNGC 3 cut(s) 139, 796, 967
Bme18I GGWCC 3 cut(s) 422, 764, 781
BmgT120I GGNCC 4 cut(s) 422, 764, 781, 853
BmiI GGNNCC 2 cut(s) 335, 424
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 1 cut(s) 42
BmuI ACTGGG 1 cut(s) 724
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 1103
BpuEI CTTGAG 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 520
BsaWI WCCGGW 1 cut(s) 356
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 980
Bse1I ACTGG 5 cut(s) 16, 131, 719, 899, 1086
BseDI CCNNGG 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 980
BseMII CTCAG 3 cut(s) 321, 328, 525
BseNI ACTGG 5 cut(s) 16, 131, 719, 899, 1086
BseXI GCAGC 3 cut(s) 124, 781, 977
BsgI GTGCAG 1 cut(s) 693
BshFI GGCC 2 cut(s) 159, 854
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 998
BslI CCNNNNNNNGG 1 cut(s) 980
BsmAI GTCTC 1 cut(s) 823
BsmFI GGGAC 1 cut(s) 998
BsnI GGCC 2 cut(s) 159, 854
Bsp1286I GDGCHC 3 cut(s) 292, 681, 934
Bsp143I GATC 1 cut(s) 1004
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 3 cut(s) 24, 588, 913
BspANI GGCC 2 cut(s) 159, 854
BspCNI CTCAG 3 cut(s) 320, 327, 526
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 2 cut(s) 335, 424
BspMAI CTGCAG 3 cut(s) 624, 666, 1156
BspPI GGATC 1 cut(s) 1012
BspQI GCTCTTC 1 cut(s) 747
BsrI ACTGG 5 cut(s) 16, 131, 719, 899, 1086
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 1 cut(s) 1004
BssT1I CCWWGG 1 cut(s) 520
Bst4CI ACNGT 3 cut(s) 237, 612, 733
Bst6I CTCTTC 1 cut(s) 747
BstC8I GCNNGC 5 cut(s) 592, 596, 630, 890, 1131
BstDEI CTNAG 5 cut(s) 307, 314, 329, 534, 758
BstDSI CCRYGG 1 cut(s) 520
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 1 cut(s) 1007
BstMAI GTCTC 1 cut(s) 823
BstMBI GATC 1 cut(s) 1004
BstMWI GCNNNNNNNGC 3 cut(s) 21, 557, 566
BstNSI RCATGY 1 cut(s) 892
BstSFI CTRYAG 3 cut(s) 620, 662, 1152
BstV1I GCAGC 3 cut(s) 124, 781, 977
BsuI GTATCC 1 cut(s) 704
BsuRI GGCC 2 cut(s) 159, 854
BtgI CCRYGG 1 cut(s) 520
BtsIMutI CAGTG 2 cut(s) 322, 892
Cac8I GCNNGC 5 cut(s) 592, 596, 630, 890, 1131
CciI TCATGA 1 cut(s) 847
CfoI GCGC 1 cut(s) 507
Cfr13I GGNCC 4 cut(s) 422, 764, 781, 853
Csp6I GTAC 2 cut(s) 28, 470
CviAII CATG 9 cut(s) 75, 225, 521, 848, 889, 943, 979, 1045, 1206
CviQI GTAC 2 cut(s) 28, 470
DdeI CTNAG 5 cut(s) 307, 314, 329, 534, 758
DpnI GATC 1 cut(s) 1006
DpnII GATC 1 cut(s) 1004
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 520
Eco24I GRGCYC 2 cut(s) 681, 934
Eco47I GGWCC 3 cut(s) 422, 764, 781
Eco57I CTGAAG 1 cut(s) 771
EcoO109I RGGNCCY 2 cut(s) 781, 853
EcoT14I CCWWGG 1 cut(s) 520
EcoT38I GRGCYC 2 cut(s) 681, 934
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 9 cut(s) 78, 228, 524, 851, 892, 946, 982, 1048, 1209
FaqI GGGAC 1 cut(s) 998
FatI CATG 9 cut(s) 74, 224, 520, 847, 888, 942, 978, 1044, 1205
FauI CCCGC 1 cut(s) 17
FauNDI CATATG 2 cut(s) 721, 828
Fnu4HI GCNGC 3 cut(s) 138, 795, 966
FriOI GRGCYC 2 cut(s) 681, 934
Fsp4HI GCNGC 3 cut(s) 138, 795, 966
FspBI CTAG 4 cut(s) 278, 473, 602, 1077
GlaI GCGC 1 cut(s) 506
GluI GCNGC 3 cut(s) 138, 795, 966
GsuI CTGGAG 1 cut(s) 1103
HaeIII GGCC 2 cut(s) 159, 854
HapII CCGG 1 cut(s) 357
HhaI GCGC 1 cut(s) 507
Hin1II CATG 9 cut(s) 78, 228, 524, 851, 892, 946, 982, 1048, 1209
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HincII GTYRAC 3 cut(s) 615, 1011, 1171
HindII GTYRAC 3 cut(s) 615, 1011, 1171
HindIII AAGCTT 1 cut(s) 651
HinfI GANTC 3 cut(s) 165, 320, 696
HpaI GTTAAC 1 cut(s) 1011
HpaII CCGG 1 cut(s) 357
HphI GGTGA 2 cut(s) 19, 346
Hpy166II GTNNAC 4 cut(s) 615, 994, 1011, 1171
Hpy188I TCNGA 5 cut(s) 51, 173, 310, 739, 929
Hpy188III TCNNGA 3 cut(s) 241, 749, 848
Hpy8I GTNNAC 4 cut(s) 615, 994, 1011, 1171
HpyAV CCTTC 3 cut(s) 562, 755, 944
HpyCH4III ACNGT 3 cut(s) 237, 612, 733
HpyCH4V TGCA 9 cut(s) 560, 582, 622, 664, 674, 839, 892, 1154, 1199
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 557, 566
HpyF3I CTNAG 5 cut(s) 307, 314, 329, 534, 758
Hsp92II CATG 9 cut(s) 78, 228, 524, 851, 892, 946, 982, 1048, 1209
HspAI GCGC 1 cut(s) 505
KspAI GTTAAC 1 cut(s) 1011
Kzo9I GATC 1 cut(s) 1004
LguI GCTCTTC 1 cut(s) 747
LmnI GCTCC 1 cut(s) 339
Lsp1109I GCAGC 3 cut(s) 124, 781, 977
LweI GCATC 1 cut(s) 42
MaeI CTAG 4 cut(s) 278, 473, 602, 1077
MaeIII GTNAC 5 cut(s) 7, 361, 436, 634, 958
MalI GATC 1 cut(s) 1006
MboI GATC 1 cut(s) 1004
MboII GAAGA 2 cut(s) 266, 764
MfeI CAATTG 1 cut(s) 19
MhlI GDGCHC 3 cut(s) 292, 681, 934
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 4 cut(s) 19, 508, 843, 1189
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 1 cut(s) 705
MmeI TCCRAC 2 cut(s) 50, 416
MnlI CCTC 8 cut(s) 101, 436, 639, 794, 844, 909, 935, 1032
Mox20I TGGCCA 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 744
MscI TGGCCA 1 cut(s) 159
MseI TTAA 7 cut(s) 303, 477, 777, 873, 1010, 1056, 1179
MslI CAYNNNNRTG 1 cut(s) 359
Msp20I TGGCCA 1 cut(s) 159
MspI CCGG 1 cut(s) 357
MunI CAATTG 1 cut(s) 19
MwoI GCNNNNNNNGC 3 cut(s) 21, 557, 566
NcoI CCATGG 1 cut(s) 520
NdeI CATATG 2 cut(s) 721, 828
NdeII GATC 1 cut(s) 1004
NlaIII CATG 9 cut(s) 78, 228, 524, 851, 892, 946, 982, 1048, 1209
NlaIV GGNNCC 2 cut(s) 335, 424
NmuCI GTSAC 4 cut(s) 7, 361, 436, 958
NspI RCATGY 1 cut(s) 892
OliI CACNNNNGTG 1 cut(s) 359
PaeI GCATGC 1 cut(s) 892
PagI TCATGA 1 cut(s) 847
PciSI GCTCTTC 1 cut(s) 747
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 2 cut(s) 165, 320
PkrI GCNGC 3 cut(s) 139, 796, 967
PleI GAGTC 1 cut(s) 704
PpsI GAGTC 1 cut(s) 704
PpuMI RGGWCCY 1 cut(s) 781
PsiI TTATAA 1 cut(s) 1139
Psp5II RGGWCCY 1 cut(s) 781
PspN4I GGNNCC 2 cut(s) 335, 424
PspPI GGNCC 4 cut(s) 422, 764, 781, 853
PspPPI RGGWCCY 1 cut(s) 781
PstI CTGCAG 3 cut(s) 624, 666, 1156
RsaI GTAC 2 cut(s) 29, 471
RsaNI GTAC 2 cut(s) 28, 470
RseI CAYNNNNRTG 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 747
SaqAI TTAA 7 cut(s) 303, 477, 777, 873, 1010, 1056, 1179
SatI GCNGC 3 cut(s) 138, 795, 966
Sau3AI GATC 1 cut(s) 1004
Sau96I GGNCC 4 cut(s) 422, 764, 781, 853
SchI GAGTC 1 cut(s) 705
SduI GDGCHC 3 cut(s) 292, 681, 934
SfaNI GCATC 1 cut(s) 42
SfcI CTRYAG 3 cut(s) 620, 662, 1152
SinI GGWCC 3 cut(s) 422, 764, 781
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SpeI ACTAGT 1 cut(s) 472
SphI GCATGC 1 cut(s) 892
Sse9I AATT 4 cut(s) 19, 508, 843, 1189
SsiI CCGC 3 cut(s) 24, 588, 913
SspI AATATT 1 cut(s) 1091
SspMI CTAG 4 cut(s) 278, 473, 602, 1077
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 3 cut(s) 237, 612, 733
TaqI TCGA 2 cut(s) 242, 1163
TasI AATT 4 cut(s) 19, 508, 843, 1189
TfiI GAWTC 2 cut(s) 165, 320
Tru1I TTAA 7 cut(s) 303, 477, 777, 873, 1010, 1056, 1179
Tru9I TTAA 7 cut(s) 303, 477, 777, 873, 1010, 1056, 1179
TscAI CASTG 2 cut(s) 322, 899
TseFI GTSAC 4 cut(s) 7, 361, 436, 958
TseI GCWGC 3 cut(s) 137, 794, 965
Tsp45I GTSAC 4 cut(s) 7, 361, 436, 958
TspGWI ACGGA 1 cut(s) 802
TspRI CASTG 2 cut(s) 322, 899
VpaK11BI GGWCC 3 cut(s) 422, 764, 781
XceI RCATGY 1 cut(s) 892
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 4 cut(s) 278, 473, 602, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.