RchiOBHm_Chr7g0192031

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011559
Physical Location & Seq
Forward (+)
10655819 .. 10657942
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17160

Sequence Viewer

Length: 1353 bp
ATGGAAGGACTAGAAGGTAACACAAACAACTATGAAGCTGGTTTGAGCATTGAACAAGCTCGAACAAAGTGTGATCAAGATCATGAATATACACTCCCCTCAGCTCACGCTATTGGTCATGATTCATGGCAGCAGGTTGGGTTGATGATGGTGACTGGCTTCAATTGCGGGTACATATTGAGCTTTTCAAATCTGATGCTTGTGCCTTTGGGTTGGACATGGGGTATCATCTGCCTCGTTGTTGTTGGCTTTTATACCGCCTATGCTAACTGGCTTTTGGCTGCTTTTCACTTCATCAATGGCCAAAGATTCATCAGATACAGAGATATTATGGGCTATTTATATGGCAGAGAAATGTATTACATAACATGGGTTTTACAGTTCTCGACCCTACTTCTTGGAAATATGGGTTTCATTCTTCTAGGGGGTAGAGCACTCAAGGAGATTAACTCAGAACTCAGTGATTCTCCACTGAGGCTCCAATACTTTATTGTCATCACCGGAGTGACCTACTTCATATTCGCCTTTTTCATTCCAACAATGTCTAAAATGAGATTTTGGCTTGGACCCTCTACCATTGTCACTTTTGCTTACATTGTCATACTCTTGGTGGTACTAGTTAAAGATGGGAAAACAAAGACGACAGAGCGAAATTATGAAATCCATGGAAACAAAGCTGAGAGAGTGTTCAATGTCTTCAATGCAGTTTCAGCTATTATTGTCTGCAACACCGCAGGCTTGCTACTAGAAATACAGTCAACCCTGCAGAAGCCAGCAGTAACAAATATGAGGAAAGCTTTATATCTGCAGTATAGTGCAGGGCTCTTATTTTACTATGGAGTCAGCATAGTAGGATACTGGGCATATGGCGCAACCGTATCTGAATACCTTCCTGAAGAGCTAAGTGGTCCTAAATCGGTTAAGGTCCTCATAAACGCTGCCGTGTTTCTACAATCCATAGTCTCACAACATATGTTTGTTGCACCAATTCATGAGGCCCTTGATACCAAGTTCCTTAAACTTGATGAGAGCATGCACTGGAAATATAACTTCAAGCGGAGGTTCTACCTCCGAGCCCTTCTCTTCATGGGAAATACATTTGTGACAGCAGCTATTCCTTTCATGGGGGACTTTGTGAACTTATTTGGATCGTTTACACTCGTTCCTCTAACTTTTGTTTTTCCAAGCATGATTTTCATTAAGGTGAAGGGAAAGACAGCTAGACTGGAGAAAAATTTGTGGCATTGGTTCAACATTGTTATTTTTTCTCTGCTTGCGATTATAACTACGATTTCTGCAGTTCGTTCGATTGTCAACAATGTTAAGGAATACAATTTCTTTGCAGATACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

450

Amino Acids

51.29

Weight (kDa)

9.0

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 41 - 433 3.1e-59 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1283
Acc36I ACCTGC 1 cut(s) 124
AciI CCGC 4 cut(s) 168, 258, 732, 1057
AclWI GGATC 1 cut(s) 1156
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 1 cut(s) 1234
AcuI CTGAAG 1 cut(s) 915
AfaI GTAC 2 cut(s) 173, 615
AfiI CCNNNNNNNGG 1 cut(s) 1124
AgsI TTSAA 7 cut(s) 53, 163, 189, 691, 700, 1054, 1252
AhlI ACTAGT 1 cut(s) 616
AleI CACNNNNGTG 1 cut(s) 503
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 1 cut(s) 967
AlwI GGATC 1 cut(s) 1156
AoxI GGCC 2 cut(s) 301, 996
ApeKI GCWGC 4 cut(s) 130, 281, 938, 1109
ApoI RAATTY 1 cut(s) 1234
Asp700I GAANNNNTTC 1 cut(s) 888
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 4 cut(s) 566, 908, 925, 997
AsuHPI GGTGA 3 cut(s) 163, 490, 1216
AvaII GGWCC 3 cut(s) 566, 908, 925
BalI TGGCCA 1 cut(s) 303
BanII GRGCYC 2 cut(s) 825, 1078
BbsI GAAGAC 1 cut(s) 688
Bbv12I GWGCWC 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 100
BbvI GCAGC 4 cut(s) 142, 268, 925, 1121
BccI CCATC 2 cut(s) 142, 620
BceAI ACGGC 1 cut(s) 926
BcgI CGANNNNNNTGC 2 cut(s) 1287, 1321
BciVI GTATCC 1 cut(s) 848
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 967
BcuI ACTAGT 1 cut(s) 616
BfaI CTAG 5 cut(s) 11, 422, 617, 746, 1221
BfmI CTRYAG 3 cut(s) 764, 806, 1296
BfuAI ACCTGC 1 cut(s) 124
BfuI GTATCC 1 cut(s) 848
BisI GCNGC 4 cut(s) 131, 282, 939, 1110
BlsI GCNGC 4 cut(s) 132, 283, 940, 1111
Bme18I GGWCC 3 cut(s) 566, 908, 925
BmgT120I GGNCC 4 cut(s) 566, 908, 925, 997
BmiI GGNNCC 2 cut(s) 479, 568
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 868
BpiI GAAGAC 1 cut(s) 688
BplI GAGNNNNNCTC 4 cut(s) 434, 466, 1065, 1097
BpmI CTGGAG 1 cut(s) 1247
Bpu10I CCTNAGC 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 664
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 1124
Bse1I ACTGG 5 cut(s) 160, 275, 863, 1043, 1230
Bse8I GATNNNNATC 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 664
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 1 cut(s) 1124
BseMII CTCAG 5 cut(s) 114, 464, 465, 472, 669
BseNI ACTGG 5 cut(s) 160, 275, 863, 1043, 1230
BseXI GCAGC 4 cut(s) 142, 268, 925, 1121
BsgI GTGCAG 1 cut(s) 837
BshFI GGCC 2 cut(s) 303, 998
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 1142
BslI CCNNNNNNNGG 1 cut(s) 1124
BsmAI GTCTC 1 cut(s) 967
BsmFI GGGAC 1 cut(s) 1142
BsnI GGCC 2 cut(s) 303, 998
Bsp1286I GDGCHC 3 cut(s) 436, 825, 1078
Bsp143I GATC 3 cut(s) 73, 79, 1148
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 4 cut(s) 168, 258, 732, 1057
BspANI GGCC 2 cut(s) 303, 998
BspCNI CTCAG 5 cut(s) 113, 464, 465, 471, 670
BspHI TCATGA 3 cut(s) 82, 118, 991
BspLI GGNNCC 2 cut(s) 479, 568
BspMAI CTGCAG 3 cut(s) 768, 810, 1300
BspMI ACCTGC 1 cut(s) 124
BspPI GGATC 1 cut(s) 1156
BspQI GCTCTTC 1 cut(s) 891
BsrI ACTGG 5 cut(s) 160, 275, 863, 1043, 1230
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 3 cut(s) 73, 79, 1148
BssT1I CCWWGG 1 cut(s) 664
Bst4CI ACNGT 3 cut(s) 381, 756, 877
Bst6I CTCTTC 2 cut(s) 891, 1088
BstC8I GCNNGC 5 cut(s) 736, 740, 774, 1034, 1275
BstDEI CTNAG 6 cut(s) 100, 451, 458, 473, 678, 902
BstDSI CCRYGG 1 cut(s) 664
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 3 cut(s) 76, 82, 1151
BstMAI GTCTC 1 cut(s) 967
BstMBI GATC 3 cut(s) 73, 79, 1148
BstMWI GCNNNNNNNGC 3 cut(s) 165, 710, 869
BstNSI RCATGY 1 cut(s) 1036
BstSFI CTRYAG 3 cut(s) 764, 806, 1296
BstV1I GCAGC 4 cut(s) 142, 268, 925, 1121
BstV2I GAAGAC 1 cut(s) 688
BsuI GTATCC 1 cut(s) 848
BsuRI GGCC 2 cut(s) 303, 998
BtgI CCRYGG 1 cut(s) 664
BtsIMutI CAGTG 3 cut(s) 466, 470, 1036
BveI ACCTGC 1 cut(s) 124
Cac8I GCNNGC 5 cut(s) 736, 740, 774, 1034, 1275
CciI TCATGA 3 cut(s) 82, 118, 991
CfoI GCGC 1 cut(s) 872
Cfr13I GGNCC 4 cut(s) 566, 908, 925, 997
Csp6I GTAC 2 cut(s) 172, 614
CviQI GTAC 2 cut(s) 172, 614
DdeI CTNAG 6 cut(s) 100, 451, 458, 473, 678, 902
DpnI GATC 3 cut(s) 75, 81, 1150
DpnII GATC 3 cut(s) 73, 79, 1148
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 891, 1088
EarI CTCTTC 2 cut(s) 891, 1088
Eco130I CCWWGG 1 cut(s) 664
Eco24I GRGCYC 2 cut(s) 825, 1078
Eco47I GGWCC 3 cut(s) 566, 908, 925
Eco57I CTGAAG 1 cut(s) 915
EcoO109I RGGNCCY 2 cut(s) 925, 997
EcoT14I CCWWGG 1 cut(s) 664
EcoT38I GRGCYC 2 cut(s) 825, 1078
ErhI CCWWGG 1 cut(s) 664
FaqI GGGAC 1 cut(s) 1142
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 2 cut(s) 865, 972
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 4 cut(s) 131, 282, 939, 1110
FriOI GRGCYC 2 cut(s) 825, 1078
Fsp4HI GCNGC 4 cut(s) 131, 282, 939, 1110
FspBI CTAG 5 cut(s) 11, 422, 617, 746, 1221
GlaI GCGC 1 cut(s) 871
GluI GCNGC 4 cut(s) 131, 282, 939, 1110
GsuI CTGGAG 1 cut(s) 1247
HaeIII GGCC 2 cut(s) 303, 998
HapII CCGG 1 cut(s) 501
HhaI GCGC 1 cut(s) 872
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HincII GTYRAC 2 cut(s) 759, 1315
HindII GTYRAC 2 cut(s) 759, 1315
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 4 cut(s) 122, 309, 464, 840
HpaII CCGG 1 cut(s) 501
HphI GGTGA 3 cut(s) 163, 490, 1216
Hpy166II GTNNAC 4 cut(s) 759, 1138, 1155, 1315
Hpy188I TCNGA 5 cut(s) 195, 317, 454, 883, 1073
Hpy188III TCNNGA 6 cut(s) 77, 83, 119, 385, 893, 992
Hpy8I GTNNAC 4 cut(s) 759, 1138, 1155, 1315
HpyAV CCTTC 4 cut(s) 8, 899, 1088, 1201
HpyCH4III ACNGT 3 cut(s) 381, 756, 877
HpyCH4V TGCA 9 cut(s) 704, 726, 766, 808, 818, 983, 1036, 1298, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 710, 869
HpyF3I CTNAG 6 cut(s) 100, 451, 458, 473, 678, 902
HspAI GCGC 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 73, 79, 1148
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 1 cut(s) 483
Lsp1109I GCAGC 4 cut(s) 142, 268, 925, 1121
LweI GCATC 1 cut(s) 186
MaeI CTAG 5 cut(s) 11, 422, 617, 746, 1221
MaeIII GTNAC 6 cut(s) 17, 151, 505, 580, 778, 1102
MalI GATC 3 cut(s) 75, 81, 1150
MboI GATC 3 cut(s) 73, 79, 1148
MboII GAAGA 4 cut(s) 410, 688, 908, 1075
MfeI CAATTG 1 cut(s) 163
MhlI GDGCHC 3 cut(s) 436, 825, 1078
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 5 cut(s) 163, 652, 987, 1234, 1333
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 1 cut(s) 849
MmeI TCCRAC 2 cut(s) 194, 560
Mox20I TGGCCA 1 cut(s) 303
MroXI GAANNNNTTC 1 cut(s) 888
MscI TGGCCA 1 cut(s) 303
MseI TTAA 6 cut(s) 447, 621, 921, 1017, 1200, 1323
MslI CAYNNNNRTG 2 cut(s) 503, 1202
Msp20I TGGCCA 1 cut(s) 303
MspI CCGG 1 cut(s) 501
MunI CAATTG 1 cut(s) 163
MwoI GCNNNNNNNGC 3 cut(s) 165, 710, 869
NcoI CCATGG 1 cut(s) 664
NdeI CATATG 2 cut(s) 865, 972
NdeII GATC 3 cut(s) 73, 79, 1148
NlaIV GGNNCC 2 cut(s) 479, 568
NmuCI GTSAC 4 cut(s) 151, 505, 580, 1102
NspI RCATGY 1 cut(s) 1036
OliI CACNNNNGTG 1 cut(s) 503
PaeI GCATGC 1 cut(s) 1036
PagI TCATGA 3 cut(s) 82, 118, 991
PciSI GCTCTTC 1 cut(s) 891
PdmI GAANNNNTTC 1 cut(s) 888
PfeI GAWTC 3 cut(s) 122, 309, 464
PkrI GCNGC 4 cut(s) 132, 283, 940, 1111
PleI GAGTC 1 cut(s) 848
PpsI GAGTC 1 cut(s) 848
PpuMI RGGWCCY 1 cut(s) 925
PsiI TTATAA 1 cut(s) 1283
Psp5II RGGWCCY 1 cut(s) 925
PspN4I GGNNCC 2 cut(s) 479, 568
PspPI GGNCC 4 cut(s) 566, 908, 925, 997
PspPPI RGGWCCY 1 cut(s) 925
PstI CTGCAG 3 cut(s) 768, 810, 1300
RsaI GTAC 2 cut(s) 173, 615
RsaNI GTAC 2 cut(s) 172, 614
RseI CAYNNNNRTG 2 cut(s) 503, 1202
SapI GCTCTTC 1 cut(s) 891
SaqAI TTAA 6 cut(s) 447, 621, 921, 1017, 1200, 1323
SatI GCNGC 4 cut(s) 131, 282, 939, 1110
Sau3AI GATC 3 cut(s) 73, 79, 1148
Sau96I GGNCC 4 cut(s) 566, 908, 925, 997
SchI GAGTC 1 cut(s) 849
SduI GDGCHC 3 cut(s) 436, 825, 1078
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 3 cut(s) 764, 806, 1296
SinI GGWCC 3 cut(s) 566, 908, 925
SmiMI CAYNNNNRTG 2 cut(s) 503, 1202
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SpeI ACTAGT 1 cut(s) 616
SphI GCATGC 1 cut(s) 1036
Sse9I AATT 5 cut(s) 163, 652, 987, 1234, 1333
SsiI CCGC 4 cut(s) 168, 258, 732, 1057
SspMI CTAG 5 cut(s) 11, 422, 617, 746, 1221
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 3 cut(s) 381, 756, 877
TaqI TCGA 3 cut(s) 61, 386, 1307
TasI AATT 5 cut(s) 163, 652, 987, 1234, 1333
TfiI GAWTC 3 cut(s) 122, 309, 464
Tru1I TTAA 6 cut(s) 447, 621, 921, 1017, 1200, 1323
Tru9I TTAA 6 cut(s) 447, 621, 921, 1017, 1200, 1323
TscAI CASTG 3 cut(s) 466, 477, 1043
TseFI GTSAC 4 cut(s) 151, 505, 580, 1102
TseI GCWGC 4 cut(s) 130, 281, 938, 1109
Tsp45I GTSAC 4 cut(s) 151, 505, 580, 1102
TspRI CASTG 3 cut(s) 466, 477, 1043
VpaK11BI GGWCC 3 cut(s) 566, 908, 925
XapI RAATTY 1 cut(s) 1234
XceI RCATGY 1 cut(s) 1036
XmnI GAANNNNTTC 1 cut(s) 888
XspI CTAG 5 cut(s) 11, 422, 617, 746, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.