FvH4_5g07330

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010751
Physical Location & Seq
Forward (+)
4287195 .. 4290157
2963 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07330.t3

Sequence Viewer

Length: 810 bp
ATGAAGAACACGACGAGCCTATTTATTTCGATTCTTTTCCTGTTATTGTTTTCCTCCTCTGCCCAGAAAAACCAGGATTCTGAAATCTTTGCAGCTTCAAGAACACTTCTACAAGAAGAAGAGCATGCTCATGAAGTACATTGTTCGAGAGAAAGAAGTAGGGCGGCATGGAAGATCATAGAGGAGTATTTGATGCCATTTGTTAAAAAAGAACGCTATGAGATCTCAACCAAGTGTCGCCTTCATCCTGATAATGATCTATTTAGGGATCAGGAACGACACAAGATTCATTTGGATATAAATGAATGGCAGTGTGGATACTGTAAGAAGAGCTTCCGTGCTGAGAAGTTTCTTGATCAGCATTTGGACAACAGACACTACAATCTTCTGAATGTAAGTGGAAGCAAGTGCTTAGCTGATCTATGCGGAGCTTTGCATTGTGACACCGTAATGAATACCCAATCAAGCAAAAAAAAGTGCAATCCTGCAGCTACTGCAAGGAATCACCACCTATGTGAGGGTCTTGCTAATAGCTGTTTTCCCTCAGATCAGGGGCCCTCTGCACACCGTCTTAATGAATTGTTCTTGCACCAGTTCTGTCATTCTCACACTTGCTCAGAAAAGCAGAAACCCTTTTCTAGAGGAGGACAGAAACAAGTGCATGTATTCTACCTGGCTATTTCCATACTGACTCTGATGTTGCTTCCTATTTTCTATGTGATACTTTATTTACACCAAAGAGAGATGAGAAAGGGAACCCAAGAGCTCAGGCGCATACCAAAAGTTGGGCTAAAAAAGAAACCCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

31.28

Weight (kDa)

9.11

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 785
AciI CCGC 2 cut(s) 164, 426
AclWI GGATC 1 cut(s) 276
AfaI GTAC 1 cut(s) 138
AfiI CCNNNNNNNGG 2 cut(s) 517, 785
AgsI TTSAA 1 cut(s) 99
AjnI CCWGG 2 cut(s) 72, 672
AleI CACNNNNGTG 1 cut(s) 513
AluBI AGCT 7 cut(s) 95, 333, 416, 431, 491, 534, 766
AluI AGCT 7 cut(s) 95, 333, 416, 431, 491, 534, 766
Alw21I GWGCWC 1 cut(s) 768
AlwI GGATC 1 cut(s) 276
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 1 cut(s) 554
ApaI GGGCCC 1 cut(s) 558
ApeKI GCWGC 2 cut(s) 92, 488
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 1 cut(s) 774
AspS9I GGNCC 2 cut(s) 554, 555
AsuHPI GGTGA 1 cut(s) 497
BaeGI GKGCMC 1 cut(s) 558
BanII GRGCYC 2 cut(s) 558, 768
Bbv12I GWGCWC 1 cut(s) 768
BbvI GCAGC 2 cut(s) 104, 500
BciT130I CCWGG 2 cut(s) 74, 674
BciVI GTATCC 1 cut(s) 311
BclI TGATCA 1 cut(s) 355
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 1 cut(s) 486
BfuI GTATCC 1 cut(s) 311
BglII AGATCT 1 cut(s) 222
BisI GCNGC 3 cut(s) 93, 165, 489
BlpI GCTNAGC 1 cut(s) 412
BlsI GCNGC 3 cut(s) 94, 166, 490
Bme1390I CCNGG 2 cut(s) 74, 674
BmgT120I GGNCC 2 cut(s) 554, 555
BmiI GGNNCC 3 cut(s) 555, 556, 757
BmrFI CCNGG 2 cut(s) 74, 674
BmsI GCATC 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 767
Bpu1102I GCTNAGC 1 cut(s) 412
BsaBI GATNNNNATC 1 cut(s) 255
Bsc4I CCNNNNNNNGG 2 cut(s) 517, 785
Bse1I ACTGG 1 cut(s) 592
Bse8I GATNNNNATC 1 cut(s) 255
BseBI CCWGG 2 cut(s) 74, 674
BseGI GGATG 1 cut(s) 244
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 2 cut(s) 517, 785
BseMII CTCAG 4 cut(s) 333, 558, 630, 781
BseNI ACTGG 1 cut(s) 592
BseRI GAGGAG 3 cut(s) 46, 197, 657
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 2 cut(s) 104, 500
BsgI GTGCAG 1 cut(s) 546
BshFI GGCC 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 768
BslI CCNNNNNNNGG 2 cut(s) 517, 785
BsnI GGCC 1 cut(s) 556
Bsp120I GGGCCC 1 cut(s) 554
Bsp1286I GDGCHC 2 cut(s) 558, 768
Bsp143I GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
Bsp1720I GCTNAGC 1 cut(s) 412
BspACI CCGC 2 cut(s) 164, 426
BspANI GGCC 1 cut(s) 556
BspCNI CTCAG 4 cut(s) 334, 557, 629, 780
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 3 cut(s) 555, 556, 757
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 1 cut(s) 276
BspQI GCTCTTC 2 cut(s) 114, 323
BsrI ACTGG 1 cut(s) 592
BssMI GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
Bst2UI CCWGG 2 cut(s) 74, 674
Bst4CI ACNGT 3 cut(s) 323, 448, 569
Bst6I CTCTTC 2 cut(s) 114, 323
BstAPI GCANNNNNTGC 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 5 cut(s) 342, 412, 544, 616, 767
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 1 cut(s) 244
BstHHI GCGC 1 cut(s) 774
BstKTI GATC 7 cut(s) 177, 225, 259, 271, 358, 421, 550
BstMBI GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstNI CCWGG 2 cut(s) 74, 674
BstNSI RCATGY 2 cut(s) 128, 665
BstSCI CCNGG 2 cut(s) 72, 672
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 104, 500
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuI GTATCC 1 cut(s) 311
BsuRI GGCC 1 cut(s) 556
BtsCI GGATG 1 cut(s) 244
BtsI GCAGTG 1 cut(s) 317
BtsIMutI CAGTG 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 494
CciI TCATGA 1 cut(s) 130
CfoI GCGC 1 cut(s) 774
Cfr13I GGNCC 2 cut(s) 554, 555
Csp6I GTAC 1 cut(s) 137
CviAII CATG 4 cut(s) 125, 131, 168, 662
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 5 cut(s) 342, 412, 544, 616, 767
DpnI GATC 7 cut(s) 176, 224, 258, 270, 357, 420, 549
DpnII GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
Eam1104I CTCTTC 2 cut(s) 114, 323
EarI CTCTTC 2 cut(s) 114, 323
Ecl136II GAGCTC 1 cut(s) 766
Eco24I GRGCYC 2 cut(s) 558, 768
Eco53kI GAGCTC 1 cut(s) 766
EcoICRI GAGCTC 1 cut(s) 766
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoO109I RGGNCCY 2 cut(s) 554, 555
EcoRII CCWGG 2 cut(s) 72, 672
EcoT38I GRGCYC 2 cut(s) 558, 768
FaeI CATG 4 cut(s) 128, 134, 171, 665
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FatI CATG 4 cut(s) 124, 130, 167, 661
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 3 cut(s) 93, 165, 489
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 2 cut(s) 558, 768
Fsp4HI GCNGC 3 cut(s) 93, 165, 489
FspBI CTAG 1 cut(s) 639
GlaI GCGC 1 cut(s) 773
GluI GCNGC 3 cut(s) 93, 165, 489
HaeIII GGCC 1 cut(s) 556
HhaI GCGC 1 cut(s) 774
Hin1II CATG 4 cut(s) 128, 134, 171, 665
Hin6I GCGC 1 cut(s) 772
HinP1I GCGC 1 cut(s) 772
HinfI GANTC 5 cut(s) 31, 77, 286, 502, 691
HphI GGTGA 1 cut(s) 497
Hpy188I TCNGA 5 cut(s) 82, 390, 547, 619, 696
Hpy188III TCNNGA 7 cut(s) 99, 131, 147, 248, 272, 353, 639
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 3 cut(s) 323, 448, 569
HpyCH4V TGCA 8 cut(s) 92, 436, 480, 488, 497, 563, 589, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 5 cut(s) 342, 412, 544, 616, 767
Hsp92II CATG 4 cut(s) 128, 134, 171, 665
HspAI GCGC 1 cut(s) 772
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
LguI GCTCTTC 2 cut(s) 114, 323
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 2 cut(s) 104, 500
LweI GCATC 1 cut(s) 183
MaeI CTAG 1 cut(s) 639
MaeIII GTNAC 1 cut(s) 440
MalI GATC 7 cut(s) 176, 224, 258, 270, 357, 420, 549
MboI GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
MboII GAAGA 6 cut(s) 16, 128, 131, 184, 340, 377
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 2 cut(s) 558, 768
MluCI AATT 1 cut(s) 578
MlyI GAGTC 1 cut(s) 685
MnlI CCTC 8 cut(s) 64, 67, 175, 511, 553, 568, 635, 638
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 2 cut(s) 204, 573
MslI CAYNNNNRTG 3 cut(s) 129, 449, 513
MspR9I CCNGG 2 cut(s) 74, 674
MvaI CCWGG 2 cut(s) 74, 674
MwoI GCNNNNNNNGC 1 cut(s) 494
NdeII GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
NlaIII CATG 4 cut(s) 128, 134, 171, 665
NlaIV GGNNCC 3 cut(s) 555, 556, 757
NmuCI GTSAC 1 cut(s) 440
NspI RCATGY 2 cut(s) 128, 665
OliI CACNNNNGTG 1 cut(s) 513
PaeI GCATGC 1 cut(s) 128
PagI TCATGA 1 cut(s) 130
PciSI GCTCTTC 2 cut(s) 114, 323
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 4 cut(s) 31, 77, 286, 502
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 3 cut(s) 94, 166, 490
PleI GAGTC 1 cut(s) 685
PpsI GAGTC 1 cut(s) 685
Psp124BI GAGCTC 1 cut(s) 768
Psp6I CCWGG 2 cut(s) 72, 672
PspGI CCWGG 2 cut(s) 72, 672
PspN4I GGNNCC 3 cut(s) 555, 556, 757
PspOMI GGGCCC 1 cut(s) 554
PspPI GGNCC 2 cut(s) 554, 555
PstI CTGCAG 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 222
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
RseI CAYNNNNRTG 3 cut(s) 129, 449, 513
SacI GAGCTC 1 cut(s) 768
SapI GCTCTTC 2 cut(s) 114, 323
SaqAI TTAA 2 cut(s) 204, 573
SatI GCNGC 3 cut(s) 93, 165, 489
Sau3AI GATC 7 cut(s) 174, 222, 256, 268, 355, 418, 547
Sau96I GGNCC 2 cut(s) 554, 555
SchI GAGTC 1 cut(s) 685
ScrFI CCNGG 2 cut(s) 74, 674
SduI GDGCHC 2 cut(s) 558, 768
SetI ASST 9 cut(s) 97, 335, 418, 433, 493, 513, 536, 675, 768
SfaNI GCATC 1 cut(s) 183
SfcI CTRYAG 1 cut(s) 486
SmiMI CAYNNNNRTG 3 cut(s) 129, 449, 513
SphI GCATGC 1 cut(s) 128
Sse9I AATT 1 cut(s) 578
SsiI CCGC 2 cut(s) 164, 426
SspMI CTAG 1 cut(s) 639
SstI GAGCTC 1 cut(s) 768
StyD4I CCNGG 2 cut(s) 72, 672
TaaI ACNGT 3 cut(s) 323, 448, 569
TaqI TCGA 2 cut(s) 29, 146
TasI AATT 1 cut(s) 578
TatI WGTACW 1 cut(s) 136
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 4 cut(s) 31, 77, 286, 502
Tru1I TTAA 2 cut(s) 204, 573
Tru9I TTAA 2 cut(s) 204, 573
TscAI CASTG 1 cut(s) 317
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 92, 488
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 7 cut(s) 17, 147, 233, 278, 318, 467, 591
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 1 cut(s) 317
Van91I CCANNNNNTGG 1 cut(s) 785
XagI CCTNNNNNAGG 1 cut(s) 515
XbaI TCTAGA 1 cut(s) 638
XceI RCATGY 2 cut(s) 128, 665
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.