Prupe.5G157300_v2.0.a1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0010751
Physical Location & Seq
Reverse (-)
13973307 .. 13977293
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G157300.4

Sequence Viewer

Length: 831 bp
ATGAAGAACACCAGCCTTTTTATTCCAATTCTTTTCTTGTTATCGTTTTCCCTTCTACAAACAGGCCAAGTCTCCTCCTTTACACAGAGAAACCAGGAGTTTGAAAAATCTGCATCTTCAAGAACCCTTGAACAAGAACACGAGCATGCTCATGAAGTACATTGTTCCAGAGAAAGAAGTAGGGCAGCATGGAAAATTATTGAGGAGTACCTGATGCCGTTTCTGGAACAAGAGCACTATGAGATGTCAAGTAAGTGTCGACTTCATCCTGACAATGATCTATTTAGGGATCAGGAACAGCACAAGATTCATCTGGATATAAACGAATGGCAGTGTGGATACTGTAAGAAAAGCTTCCGTGCTGAAAAGTTTCTTGATCAGCATTTTGACAACAGACACTACAATCTTCTGAATGTAAGTGGCAGCAAGTGCTTAGCTGATTTATGCGGAGCTTTGCATTGTGATGTCGTAATGAATACCAAGTCAAGCAAGACCAAATGCAATCCTGCAGCTGCTGCAAGGAATCACCATCTATGTGAGGGTCTTGCCGATAGCTGTTTTCCTATTAATCAAGGTCCCTCAGCACGCCGCCTTAATGAATTGTTCTTGCACCAATTTTGTCACGCTCACACGTGCTCGAGGAAACATAAACATTTTCCTAGAGGAGGAAAGAAGCAATTAAGTGTATTCTATCTGGCTATTTCCATACTGACTTTGATGCTGCTCCCTATTTTCTATCTGATAGTTTATTTGCACCAAAGAGAGATGAGAACGGGAACCCAAGATCTTAGGCGCATCTCAAAAGTTGGGCAGAAGACGAAACCCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

277

Amino Acids

32.14

Weight (kDa)

8.97

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 259
AciI CCGC 2 cut(s) 447, 589
AclWI GGATC 1 cut(s) 297
AcvI CACGTG 1 cut(s) 633
AfaI GTAC 2 cut(s) 159, 209
AfiI CCNNNNNNNGG 1 cut(s) 665
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 3 cut(s) 104, 120, 131
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 5 cut(s) 354, 437, 452, 512, 555
AluI AGCT 5 cut(s) 354, 437, 452, 512, 555
Alw21I GWGCWC 2 cut(s) 237, 638
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 297
AlwNI CAGNNNCTG 1 cut(s) 515
Ama87I CYCGRG 1 cut(s) 637
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 6 cut(s) 185, 423, 509, 512, 515, 721
AseI ATTAAT 1 cut(s) 567
Asp700I GAANNNNTTC 2 cut(s) 353, 369
AspLEI GCGC 1 cut(s) 795
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 637
AvaII GGWCC 1 cut(s) 575
BauI CACGAG 1 cut(s) 140
BbrPI CACGTG 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 821
Bbv12I GWGCWC 2 cut(s) 237, 638
BbvCI CCTCAGC 1 cut(s) 580
BbvI GCAGC 6 cut(s) 197, 435, 499, 502, 521, 708
BccI CCATC 1 cut(s) 537
BceAI ACGGC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 95
BciVI GTATCC 1 cut(s) 332
BclI TGATCA 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 1 cut(s) 660
BfmI CTRYAG 1 cut(s) 507
BfuI GTATCC 1 cut(s) 332
BglII AGATCT 1 cut(s) 784
BisI GCNGC 7 cut(s) 186, 424, 510, 513, 516, 589, 722
BlpI GCTNAGC 1 cut(s) 433
BlsI GCNGC 7 cut(s) 187, 425, 511, 514, 517, 590, 723
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 2 cut(s) 577, 778
BmrFI CCNGG 1 cut(s) 95
BmsI GCATC 4 cut(s) 122, 204, 708, 804
BpiI GAAGAC 1 cut(s) 821
Bpu10I CCTNAGC 1 cut(s) 580
Bpu1102I GCTNAGC 1 cut(s) 433
BsaAI YACGTR 1 cut(s) 633
Bsc4I CCNNNNNNNGG 1 cut(s) 665
BseBI CCWGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 665
BseMII CTCAG 1 cut(s) 594
BseRI GAGGAG 3 cut(s) 64, 218, 678
BseXI GCAGC 6 cut(s) 197, 435, 499, 502, 521, 708
BshFI GGCC 1 cut(s) 66
BsiHKAI GWGCWC 2 cut(s) 237, 638
BsiHKCI CYCGRG 1 cut(s) 637
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 1 cut(s) 665
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 561
BsnI GGCC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 637
Bsp1286I GDGCHC 2 cut(s) 237, 638
Bsp143I GATC 4 cut(s) 277, 289, 376, 784
Bsp1720I GCTNAGC 1 cut(s) 433
BspACI CCGC 2 cut(s) 447, 589
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 1 cut(s) 593
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 577, 778
BspMAI CTGCAG 1 cut(s) 511
BspPI GGATC 1 cut(s) 297
BssMI GATC 4 cut(s) 277, 289, 376, 784
BssSI CACGAG 1 cut(s) 140
Bst2BI CACGAG 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 344
BstAPI GCANNNNNTGC 2 cut(s) 429, 515
BstBAI YACGTR 1 cut(s) 633
BstC8I GCNNGC 2 cut(s) 147, 586
BstDEI CTNAG 3 cut(s) 433, 580, 788
BstENI CCTNNNNNAGG 1 cut(s) 663
BstF5I GGATG 1 cut(s) 265
BstHHI GCGC 1 cut(s) 795
BstKTI GATC 4 cut(s) 280, 292, 379, 787
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 4 cut(s) 277, 289, 376, 784
BstMWI GCNNNNNNNGC 2 cut(s) 429, 515
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 93
BstSFI CTRYAG 1 cut(s) 507
BstV1I GCAGC 6 cut(s) 197, 435, 499, 502, 521, 708
BstV2I GAAGAC 1 cut(s) 821
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 1 cut(s) 66
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 338
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 2 cut(s) 147, 586
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 795
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 2 cut(s) 158, 208
CviAII CATG 3 cut(s) 146, 152, 189
CviJI RGCY 8 cut(s) 15, 66, 354, 437, 452, 512, 555, 698
CviKI_1 RGCY 8 cut(s) 15, 66, 354, 437, 452, 512, 555, 698
CviQI GTAC 2 cut(s) 158, 208
DdeI CTNAG 3 cut(s) 433, 580, 788
DpnI GATC 4 cut(s) 279, 291, 378, 786
DpnII GATC 4 cut(s) 277, 289, 376, 784
Eco47I GGWCC 1 cut(s) 575
Eco72I CACGTG 1 cut(s) 633
Eco88I CYCGRG 1 cut(s) 637
EcoNI CCTNNNNNAGG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 93
FaeI CATG 3 cut(s) 149, 155, 192
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 561
FatI CATG 3 cut(s) 145, 151, 188
FbaI TGATCA 1 cut(s) 376
FblI GTMKAC 1 cut(s) 259
Fnu4HI GCNGC 7 cut(s) 186, 424, 510, 513, 516, 589, 722
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 7 cut(s) 186, 424, 510, 513, 516, 589, 722
FspBI CTAG 1 cut(s) 660
GlaI GCGC 1 cut(s) 794
GluI GCNGC 7 cut(s) 186, 424, 510, 513, 516, 589, 722
HaeIII GGCC 1 cut(s) 66
HhaI GCGC 1 cut(s) 795
Hin1II CATG 3 cut(s) 149, 155, 192
Hin6I GCGC 1 cut(s) 793
HinP1I GCGC 1 cut(s) 793
HincII GTYRAC 1 cut(s) 260
HindII GTYRAC 1 cut(s) 260
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 2 cut(s) 307, 523
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 2 cut(s) 411, 741
Hpy188III TCNNGA 8 cut(s) 120, 152, 168, 224, 269, 293, 314, 374
Hpy8I GTNNAC 1 cut(s) 260
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4IV ACGT 1 cut(s) 632
HpyCH4V TGCA 7 cut(s) 113, 457, 501, 509, 518, 610, 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 429, 515
HpyF3I CTNAG 3 cut(s) 433, 580, 788
HpySE526I ACGT 1 cut(s) 632
Hsp92II CATG 3 cut(s) 149, 155, 192
HspAI GCGC 1 cut(s) 793
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 4 cut(s) 277, 289, 376, 784
LmnI GCTCC 2 cut(s) 449, 729
Lsp1109I GCAGC 6 cut(s) 197, 435, 499, 502, 521, 708
LweI GCATC 4 cut(s) 122, 204, 708, 804
MaeI CTAG 1 cut(s) 660
MaeII ACGT 1 cut(s) 632
MaeIII GTNAC 1 cut(s) 620
MalI GATC 4 cut(s) 279, 291, 378, 786
MboI GATC 4 cut(s) 277, 289, 376, 784
MboII GAAGA 4 cut(s) 16, 108, 398, 826
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 237, 638
MluCI AATT 5 cut(s) 27, 195, 599, 614, 677
MnlI CCTC 7 cut(s) 85, 196, 532, 589, 633, 656, 659
MroXI GAANNNNTTC 2 cut(s) 353, 369
MseI TTAA 3 cut(s) 567, 594, 680
MslI CAYNNNNRTG 4 cut(s) 144, 150, 462, 534
MspA1I CMGCKG 1 cut(s) 512
MspR9I CCNGG 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 429, 515
NdeII GATC 4 cut(s) 277, 289, 376, 784
NlaIII CATG 3 cut(s) 149, 155, 192
NlaIV GGNNCC 2 cut(s) 577, 778
NmuCI GTSAC 1 cut(s) 620
NspI RCATGY 1 cut(s) 149
PaeI GCATGC 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 637
PagI TCATGA 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 353, 369
PfeI GAWTC 2 cut(s) 307, 523
PkrI GCNGC 7 cut(s) 187, 425, 511, 514, 517, 590, 723
PmaCI CACGTG 1 cut(s) 633
PmlI CACGTG 1 cut(s) 633
Ppu21I YACGTR 1 cut(s) 633
PpuMI RGGWCCY 1 cut(s) 575
PshBI ATTAAT 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 93
PspCI CACGTG 1 cut(s) 633
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 577, 778
PspPI GGNCC 1 cut(s) 575
PspPPI RGGWCCY 1 cut(s) 575
PspXI VCTCGAGB 1 cut(s) 637
PstI CTGCAG 1 cut(s) 511
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 784
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 2 cut(s) 159, 209
RsaNI GTAC 2 cut(s) 158, 208
RseI CAYNNNNRTG 4 cut(s) 144, 150, 462, 534
SalI GTCGAC 1 cut(s) 258
SaqAI TTAA 3 cut(s) 567, 594, 680
SatI GCNGC 7 cut(s) 186, 424, 510, 513, 516, 589, 722
Sau3AI GATC 4 cut(s) 277, 289, 376, 784
Sau96I GGNCC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 2 cut(s) 237, 638
SetI ASST 8 cut(s) 213, 356, 439, 454, 514, 557, 577, 635
SfaNI GCATC 4 cut(s) 122, 204, 708, 804
SfcI CTRYAG 1 cut(s) 507
Sfr274I CTCGAG 1 cut(s) 637
SinI GGWCC 1 cut(s) 575
SlaI CTCGAG 1 cut(s) 637
SmiMI CAYNNNNRTG 4 cut(s) 144, 150, 462, 534
SmlI CTYRAG 1 cut(s) 637
SmoI CTYRAG 1 cut(s) 637
SphI GCATGC 1 cut(s) 149
Sse9I AATT 5 cut(s) 27, 195, 599, 614, 677
SsiI CCGC 2 cut(s) 447, 589
SspMI CTAG 1 cut(s) 660
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 344
TaiI ACGT 1 cut(s) 635
TaqI TCGA 2 cut(s) 259, 638
TasI AATT 5 cut(s) 27, 195, 599, 614, 677
TatI WGTACW 1 cut(s) 157
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 2 cut(s) 307, 523
Tru1I TTAA 3 cut(s) 567, 594, 680
Tru9I TTAA 3 cut(s) 567, 594, 680
TscAI CASTG 1 cut(s) 338
TseFI GTSAC 1 cut(s) 620
TseI GCWGC 6 cut(s) 185, 423, 509, 512, 515, 721
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 6 cut(s) 17, 168, 254, 299, 488, 612
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 575
VspI ATTAAT 1 cut(s) 567
XagI CCTNNNNNAGG 1 cut(s) 663
XceI RCATGY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 637
XmiI GTMKAC 1 cut(s) 259
XmnI GAANNNNTTC 2 cut(s) 353, 369
XspI CTAG 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.