FvH4_5g14276

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010569
Physical Location & Seq
Forward (+)
8082296 .. 8084510
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14276.t1

Sequence Viewer

Length: 1092 bp
ATGTTCAATTCCAGTTTCACCTATCATTACCCCAACACCACCTTCGACCCCAACTCTCAATTCTCCTATTTTCCTCCGCTAGCCTCGTCTGATCACCCACCTGGAGTCGACCCAGTTCCCAATTCAGCCCCCTACCAATTAACCCATGCTGCACTCCAATCTCAATTCCCATTTGGTCAAGACCCAAATGCAACCTCACACGCTTGGCTTGTAAACCAAGCTGACCCAATTTCATATGTACCTTCAATAAAATCTTTGAATGAGAGCTTGTTAGTGCCCACAAATTATTTGTGGAATAACAATTGGACAAATCAACCTAATGCTGCAGAGAGCTTTCCACAGCAAACCAATGCTTTTCCAGGAATGTCCAATGGTATGGACAATGTCAGTCTGCCTAGCGAAATGAGCAGCGTGGGCGGGCAAGTGATGTATGGGAGTACAAGTGTGGTTGCTGGTGAGGAGTTGGGAACAAAGAGAAGGAAGCTTATGAAAAGTGACGCGGCGGTTGGTTTTCCAAAGATTTGCGAAATGTGCAATGTCACTACTAATAGCCCTGAAGTTTACAGGATGCATTTGGCTGGGAGAAAGCATGCTGCTCAGGCTAACTTAATGCATGGAAGGGCTGGGTTGAATTCTGCTCCCAAAATCCAATCCAAGTCCAATGGCATTGTAAAGAAAGATCCAAACAGAGTCAAGTTTCCTCAGTGTGTATGGTGTGAATATTGCAAGATATTCTGTAACAGCCAGGATGCCTATGGCAAACATATAGTCGGAAGGAAACATCAGAGGAACTTGGATCAACTCAAAGAGTTGAAGAACAAAGGGAGTGCCCCGACCACAAATGTTCCATCAGGTGCAATAAATCCACAAATTGGTCCCATGGAAAAGGTTTCCCACTCAAAGGCTCCTTCCAAAGAAGATTTGGAAACGAAAAAGCAAAAGGTTATAAGTGGAGGAGCAGCAGCCAAGGAGGTTAGAACGTGCACTATTTGCAATGTGGTTTGCAACAGCCAGATAGCTTTCAGTTCACACCTTTCTGGTCAGAAGCATGCTGCAATGGCGGCGAAGCAAGTAGCAGGTTCCAGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-met

Protein Analysis

364

Amino Acids

39.41

Weight (kDa)

9.17

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 174 - 197 6.2e-08 Zinc-finger of C2H2 type
zf-met PF12874 327 - 350 7.1e-07 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 947
Acc36I ACCTGC 1 cut(s) 1067
AccB7I CCANNNNNTGG 1 cut(s) 872
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 500
AciI CCGC 5 cut(s) 77, 417, 500, 503, 1061
AclWI GGATC 2 cut(s) 674, 804
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 1 cut(s) 576
AfaI GTAC 2 cut(s) 240, 439
AfiI CCNNNNNNNGG 2 cut(s) 872, 901
AgsI TTSAA 5 cut(s) 7, 246, 259, 631, 814
AjnI CCWGG 3 cut(s) 100, 358, 744
AjuI GAANNNNNNNTTGG 4 cut(s) 26, 44, 58, 76
AluBI AGCT 5 cut(s) 221, 267, 333, 484, 1019
AluI AGCT 5 cut(s) 221, 267, 333, 484, 1019
Alw21I GWGCWC 1 cut(s) 986
Alw44I GTGCAC 1 cut(s) 982
AlwI GGATC 2 cut(s) 674, 804
ApaLI GTGCAC 1 cut(s) 982
ApeKI GCWGC 7 cut(s) 149, 323, 408, 593, 959, 962, 1052
ApoI RAATTY 1 cut(s) 631
ArsI GACNNNNNNTTYG 2 cut(s) 753, 785
AspS9I GGNCC 1 cut(s) 875
AsuHPI GGTGA 3 cut(s) 10, 86, 467
AsuNHI GCTAGC 1 cut(s) 79
AvaII GGWCC 1 cut(s) 875
BaeGI GKGCMC 3 cut(s) 279, 832, 986
Bbv12I GWGCWC 1 cut(s) 986
BbvI GCAGC 7 cut(s) 136, 310, 420, 580, 971, 974, 1039
BccI CCATC 1 cut(s) 856
BciT130I CCWGG 3 cut(s) 102, 360, 746
BclI TGATCA 1 cut(s) 91
BfaI CTAG 2 cut(s) 80, 396
BfmI CTRYAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 1067
BisI GCNGC 9 cut(s) 150, 324, 409, 501, 594, 960, 963, 1053, 1062
BlsI GCNGC 9 cut(s) 151, 325, 410, 502, 595, 961, 964, 1054, 1063
Bme1390I CCNGG 3 cut(s) 102, 360, 746
Bme18I GGWCC 1 cut(s) 875
BmgT120I GGNCC 1 cut(s) 875
BmiI GGNNCC 3 cut(s) 877, 906, 1081
BmrFI CCNGG 3 cut(s) 102, 360, 746
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 2 cut(s) 558, 739
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 107
BpmI CTGGAG 1 cut(s) 123
Bpu10I CCTNAGC 1 cut(s) 597
BsaJI CCNNGG 2 cut(s) 879, 966
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bsc4I CCNNNNNNNGG 2 cut(s) 872, 901
Bse1I ACTGG 3 cut(s) 12, 113, 1083
Bse3DI GCAATG 3 cut(s) 541, 1000, 1062
BseBI CCWGG 3 cut(s) 102, 360, 746
BseDI CCNNGG 2 cut(s) 879, 966
BseGI GGATG 2 cut(s) 573, 754
BseLI CCNNNNNNNGG 2 cut(s) 872, 901
BseMI GCAATG 3 cut(s) 541, 1000, 1062
BseMII CTCAG 2 cut(s) 611, 716
BseNI ACTGG 3 cut(s) 12, 113, 1083
BseRI GAGGAG 2 cut(s) 473, 969
BseSI GKGCMC 3 cut(s) 279, 832, 986
BseXI GCAGC 7 cut(s) 136, 310, 420, 580, 971, 974, 1039
BseYI CCCAGC 2 cut(s) 578, 623
BsgI GTGCAG 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 986
BslFI GGGAC 1 cut(s) 861
BslI CCNNNNNNNGG 2 cut(s) 872, 901
BsmFI GGGAC 1 cut(s) 861
Bsp1286I GDGCHC 3 cut(s) 279, 832, 986
Bsp143I GATC 3 cut(s) 91, 679, 796
Bsp19I CCATGG 1 cut(s) 879
BspACI CCGC 5 cut(s) 77, 417, 500, 503, 1061
BspCNI CTCAG 2 cut(s) 610, 715
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 3 cut(s) 877, 906, 1081
BspMAI CTGCAG 1 cut(s) 328
BspMI ACCTGC 1 cut(s) 1067
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 2 cut(s) 674, 804
BsrDI GCAATG 3 cut(s) 541, 1000, 1062
BsrI ACTGG 3 cut(s) 12, 113, 1083
BssECI CCNNGG 2 cut(s) 879, 966
BssMI GATC 3 cut(s) 91, 679, 796
BssT1I CCWWGG 2 cut(s) 879, 966
Bst2UI CCWGG 3 cut(s) 102, 360, 746
BstAPI GCANNNNNTGC 1 cut(s) 990
BstC8I GCNNGC 4 cut(s) 81, 419, 591, 1050
BstDEI CTNAG 2 cut(s) 597, 702
BstDSI CCRYGG 1 cut(s) 879
BstF5I GGATG 2 cut(s) 573, 754
BstFNI CGCG 1 cut(s) 500
BstKTI GATC 3 cut(s) 94, 682, 799
BstMBI GATC 3 cut(s) 91, 679, 796
BstMWI GCNNNNNNNGC 7 cut(s) 405, 414, 531, 599, 990, 1058, 1061
BstNI CCWGG 3 cut(s) 102, 360, 746
BstNSI RCATGY 2 cut(s) 593, 1052
BstSCI CCNGG 3 cut(s) 100, 358, 744
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 3 cut(s) 279, 832, 986
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 7 cut(s) 136, 310, 420, 580, 971, 974, 1039
BstX2I RGATCY 1 cut(s) 679
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 1 cut(s) 679
BtgI CCRYGG 1 cut(s) 879
BtsCI GGATG 2 cut(s) 573, 754
BtsIMutI CAGTG 2 cut(s) 710, 1090
BveI ACCTGC 1 cut(s) 1067
Cac8I GCNNGC 4 cut(s) 81, 419, 591, 1050
Cfr13I GGNCC 1 cut(s) 875
CseI GACGC 1 cut(s) 506
Csp6I GTAC 2 cut(s) 239, 438
CviAII CATG 5 cut(s) 146, 590, 614, 880, 1049
CviQI GTAC 2 cut(s) 239, 438
DdeI CTNAG 2 cut(s) 597, 702
DpnI GATC 3 cut(s) 93, 681, 798
DpnII GATC 3 cut(s) 91, 679, 796
Eco130I CCWWGG 2 cut(s) 879, 966
Eco47I GGWCC 1 cut(s) 875
Eco57I CTGAAG 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 3 cut(s) 100, 358, 744
EcoT14I CCWWGG 2 cut(s) 879, 966
EcoT22I ATGCAT 2 cut(s) 573, 615
ErhI CCWWGG 2 cut(s) 879, 966
FaeI CATG 5 cut(s) 149, 593, 617, 883, 1052
FaqI GGGAC 1 cut(s) 861
FatI CATG 5 cut(s) 145, 589, 613, 879, 1048
FauI CCCGC 1 cut(s) 410
FauNDI CATATG 1 cut(s) 235
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 9 cut(s) 150, 324, 409, 501, 594, 960, 963, 1053, 1062
FokI GGATG 2 cut(s) 580, 761
Fsp4HI GCNGC 9 cut(s) 150, 324, 409, 501, 594, 960, 963, 1053, 1062
FspBI CTAG 2 cut(s) 80, 396
GluI GCNGC 9 cut(s) 150, 324, 409, 501, 594, 960, 963, 1053, 1062
GsaI CCCAGC 2 cut(s) 582, 627
GsuI CTGGAG 1 cut(s) 123
HgaI GACGC 1 cut(s) 506
Hin1II CATG 5 cut(s) 149, 593, 617, 883, 1052
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 105, 690
HphI GGTGA 3 cut(s) 10, 86, 467
Hpy166II GTNNAC 5 cut(s) 109, 214, 562, 984, 1028
Hpy188I TCNGA 4 cut(s) 91, 773, 786, 1044
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 5 cut(s) 109, 214, 562, 984, 1028
HpyAV CCTTC 6 cut(s) 52, 252, 471, 612, 768, 918
HpyCH4IV ACGT 1 cut(s) 980
HpyF10VI GCNNNNNNNGC 7 cut(s) 405, 414, 531, 599, 990, 1058, 1061
HpyF3I CTNAG 2 cut(s) 597, 702
HpySE526I ACGT 1 cut(s) 980
Hsp92II CATG 5 cut(s) 149, 593, 617, 883, 1052
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 91, 679, 796
LmnI GCTCC 3 cut(s) 643, 910, 956
Lsp1109I GCAGC 7 cut(s) 136, 310, 420, 580, 971, 974, 1039
LweI GCATC 2 cut(s) 558, 739
MaeI CTAG 2 cut(s) 80, 396
MaeII ACGT 1 cut(s) 980
MaeIII GTNAC 3 cut(s) 494, 538, 737
MalI GATC 3 cut(s) 93, 681, 798
MboI GATC 3 cut(s) 91, 679, 796
MboII GAAGA 2 cut(s) 826, 929
MfeI CAATTG 1 cut(s) 301
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 3 cut(s) 279, 832, 986
MlyI GAGTC 2 cut(s) 114, 699
MmeI TCCRAC 1 cut(s) 751
MnlI CCTC 8 cut(s) 84, 94, 205, 451, 711, 780, 947, 964
Mph1103I ATGCAT 2 cut(s) 573, 615
MseI TTAA 2 cut(s) 140, 608
MspR9I CCNGG 3 cut(s) 102, 360, 746
MunI CAATTG 1 cut(s) 301
MvaI CCWGG 3 cut(s) 102, 360, 746
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 7 cut(s) 405, 414, 531, 599, 990, 1058, 1061
NcoI CCATGG 1 cut(s) 879
NdeI CATATG 1 cut(s) 235
NdeII GATC 3 cut(s) 91, 679, 796
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 5 cut(s) 149, 593, 617, 883, 1052
NlaIV GGNNCC 3 cut(s) 877, 906, 1081
NmuCI GTSAC 2 cut(s) 494, 538
NsiI ATGCAT 2 cut(s) 573, 615
NspI RCATGY 2 cut(s) 593, 1052
PaeI GCATGC 2 cut(s) 593, 1052
PflFI GACNNNGTC 1 cut(s) 383
PflMI CCANNNNNTGG 1 cut(s) 872
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 9 cut(s) 151, 325, 410, 502, 595, 961, 964, 1054, 1063
PleI GAGTC 2 cut(s) 113, 698
PpsI GAGTC 2 cut(s) 113, 698
PsiI TTATAA 1 cut(s) 947
Psp6I CCWGG 3 cut(s) 100, 358, 744
PspFI CCCAGC 2 cut(s) 578, 623
PspGI CCWGG 3 cut(s) 100, 358, 744
PspN4I GGNNCC 3 cut(s) 877, 906, 1081
PspPI GGNCC 1 cut(s) 875
PstI CTGCAG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 679
PsyI GACNNNGTC 1 cut(s) 383
RsaI GTAC 2 cut(s) 240, 439
RsaNI GTAC 2 cut(s) 239, 438
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 140, 608
SatI GCNGC 9 cut(s) 150, 324, 409, 501, 594, 960, 963, 1053, 1062
Sau3AI GATC 3 cut(s) 91, 679, 796
Sau96I GGNCC 1 cut(s) 875
SchI GAGTC 2 cut(s) 114, 699
ScrFI CCNGG 3 cut(s) 102, 360, 746
SduI GDGCHC 3 cut(s) 279, 832, 986
SfaNI GCATC 2 cut(s) 558, 739
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 875
SphI GCATGC 2 cut(s) 593, 1052
SsiI CCGC 5 cut(s) 77, 417, 500, 503, 1061
SspI AATATT 1 cut(s) 722
SspMI CTAG 2 cut(s) 80, 396
StyD4I CCNGG 3 cut(s) 100, 358, 744
StyI CCWWGG 2 cut(s) 879, 966
TaiI ACGT 1 cut(s) 983
TaqI TCGA 2 cut(s) 45, 108
TatI WGTACW 1 cut(s) 437
TauI GCSGC 2 cut(s) 503, 1064
Tru1I TTAA 2 cut(s) 140, 608
Tru9I TTAA 2 cut(s) 140, 608
TscAI CASTG 2 cut(s) 710, 1090
TseFI GTSAC 2 cut(s) 494, 538
TseI GCWGC 7 cut(s) 149, 323, 408, 593, 959, 962, 1052
Tsp45I GTSAC 2 cut(s) 494, 538
TspDTI ATGAA 2 cut(s) 222, 503
TspRI CASTG 2 cut(s) 710, 1090
Tth111I GACNNNGTC 1 cut(s) 383
Van91I CCANNNNNTGG 1 cut(s) 872
VneI GTGCAC 1 cut(s) 982
VpaK11BI GGWCC 1 cut(s) 875
XapI RAATTY 1 cut(s) 631
XceI RCATGY 2 cut(s) 593, 1052
XcmI CCANNNNNNNNNTGG 2 cut(s) 752, 919
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 2 cut(s) 80, 396
Zsp2I ATGCAT 2 cut(s) 573, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.