MD14G1208900.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010569
Physical Location & Seq
Reverse (-)
29524467 .. 29526794
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1208900.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGATAATCCCACCGGATTCCACCTTTCCTTCCGGTTCACAATCCCCAATCTTTATTCCTCCACAATATTCTAACAATTTTACCTATCAAATTCCCTATCCATTCAACCCCACTAACCCAATTTGGTCTTTTCCTCAACCGGTGGTTCCCTCGGCAGTAGCCGCCGCGGATCCAGCCTGTCACCCGCCCGGCACCGACCCGTATGACAATTCGGGCTTGTTCCCGTGGACCCGTGTCGGATTCCAAGGTCAGACGCCTTTTGGTGAAGACCCAAATGTCCGTCCGCAGAACTGGGTTGGCAAGCAAGCTGAACCTGTTCGATATGAAGCTTCTATCAAATCTTTGAATGAGAGTATGACTGTGCCCTCATCGGCCTCGAATTGTTCGTGGAATAGCAGTTGGACAAATCAACCTTTGGCAAATACTGCTACAATGAACACTCCACAACCAACTGTGGTTGTCCAGTCCCTGCGGTGTGATGTCTGTGATATCTATTGTACTAGCAAACCAATTTATGAGGCACACATAGCGGGAAAGAAACACCAGAAGAAAGTCGCAATGCAACATAATCCCGGTGTGACTGCTGGTGCAGAATTGGAAACAAAGAGGCGTAAGATTTTGCGGGGCGGTTCAACAGTTGATTCTGTGAGGGTTTGCACCATATGCAACATTACTTGCAATAGTGAAGAAGTTTATAATAAGCATCTATATGGAAAAAGGCACAAGGCTCAGGCTAGCTTAATGGCTCTAGATGGCGGGTCATTTATCGCTGCAGTTCGATCTCAGTTCAATGGAATATGGAAGAAAGACCCAAATAAAGCCAAGCTTGTTCAATCTGCATGGTGTGAAGTTTGCAAAATCAATTGTAACAGCAACGATGTCTACGTCGCACACCTAGTTGGAAAGAAACATAAGAAAAACTTGGAGCAACTGGGAAAGTCCAAGAATGATGGGAGTGCCTCGACCTCCAATGCTCCATCAGCTGCAACAAATGCCATAATTGGGCCAACGAAAAACCCAGGAGCAGAGTCATCAAGGGCTTACCAGCCGCAGGTTCCAAAAGAAGACTTGGAGACAAAGAAGAAAAAGGTCATATCTGGAGGAGCAACAGCTAGTGCGGTCAGAACGTGTACTGTGTGCAATGTGGTGTGCAATAGCCATACAGTTTTTAGTTCTCATCTCGCCGGTCAGAAGCACGCTGCTATGGTTAAGAAACAAGCAGAAGCCGAAGTAGCAACTAGAGGCCCTATTACTGCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-met

Protein Analysis

421

Amino Acids

45.38

Weight (kDa)

9.23

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 158 - 181 3.2e-07 Zinc-finger of C2H2 type
zf-met PF12874 218 - 241 3.1e-08 Zinc-finger of C2H2 type
zf-met PF12874 281 - 304 1.2e-07 Zinc-finger of C2H2 type
zf-met PF12874 376 - 399 2.1e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AasI GACNNNNNNGTC 1 cut(s) 275
Acc36I ACCTGC 1 cut(s) 1042
AccB1I GGYRCC 1 cut(s) 191
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 167
AclWI GGATC 2 cut(s) 164, 177
AcsI RAATTY 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 254
AfaI GTAC 2 cut(s) 499, 1132
AfiI CCNNNNNNNGG 2 cut(s) 1002, 1051
AflIII ACRYGT 1 cut(s) 1127
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 5 cut(s) 106, 346, 633, 790, 833
AjnI CCWGG 1 cut(s) 1018
AluBI AGCT 6 cut(s) 308, 329, 738, 826, 983, 1112
AluI AGCT 6 cut(s) 308, 329, 738, 826, 983, 1112
Alw26I GTCTC 1 cut(s) 1067
AlwI GGATC 2 cut(s) 164, 177
AlwNI CAGNNNCTG 1 cut(s) 469
AoxI GGCC 3 cut(s) 372, 1004, 1243
ApeKI GCWGC 3 cut(s) 770, 983, 1199
ApoI RAATTY 1 cut(s) 90
AsiGI ACCGGT 1 cut(s) 139
Asp700I GAANNNNTTC 1 cut(s) 315
AspS9I GGNCC 3 cut(s) 228, 1004, 1244
AsuC2I CCSGG 2 cut(s) 189, 573
AsuHPI GGTGA 2 cut(s) 173, 275
AsuNHI GCTAGC 1 cut(s) 734
AvaII GGWCC 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 366
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 273, 1071
BbvI GCAGC 3 cut(s) 757, 970, 1186
BccI CCATC 3 cut(s) 746, 944, 985
BciT130I CCWGG 1 cut(s) 1020
BcnI CCSGG 2 cut(s) 189, 573
BcoDI GTCTC 1 cut(s) 1067
BfaI CTAG 6 cut(s) 501, 735, 749, 896, 1113, 1239
BfmI CTRYAG 1 cut(s) 771
BfuAI ACCTGC 1 cut(s) 1042
BisI GCNGC 6 cut(s) 162, 165, 771, 984, 1049, 1200
BlsI GCNGC 6 cut(s) 163, 166, 772, 985, 1050, 1201
Bme1390I CCNGG 3 cut(s) 189, 573, 1020
Bme18I GGWCC 1 cut(s) 228
BmgT120I GGNCC 3 cut(s) 228, 1004, 1244
BmiI GGNNCC 5 cut(s) 147, 171, 193, 230, 1056
BmrFI CCNGG 3 cut(s) 189, 573, 1020
BmrI ACTGGG 2 cut(s) 301, 941
BmsI GCATC 1 cut(s) 712
BmtI GCTAGC 1 cut(s) 738
BmuI ACTGGG 2 cut(s) 301, 941
BoxI GACNNNNGTC 1 cut(s) 233
BpiI GAAGAC 2 cut(s) 273, 1071
BpmI CTGGAG 1 cut(s) 1119
Bpu10I CCTNAGC 1 cut(s) 729
BpuMI CCSGG 2 cut(s) 189, 573
BsaHI GRCGYC 1 cut(s) 254
BsaJI CCNNGG 5 cut(s) 150, 165, 224, 244, 1018
BsaWI WCCGGW 3 cut(s) 13, 32, 139
Bsc4I CCNNNNNNNGG 2 cut(s) 1002, 1051
Bse118I RCCGGY 2 cut(s) 139, 1184
Bse1I ACTGG 3 cut(s) 296, 463, 936
Bse3DI GCAATG 2 cut(s) 564, 1147
BseBI CCWGG 1 cut(s) 1020
BseDI CCNNGG 5 cut(s) 150, 165, 224, 244, 1018
BseLI CCNNNNNNNGG 2 cut(s) 1002, 1051
BseMI GCAATG 2 cut(s) 564, 1147
BseMII CTCAG 2 cut(s) 743, 797
BseNI ACTGG 3 cut(s) 296, 463, 936
BseRI GAGGAG 1 cut(s) 1116
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 3 cut(s) 757, 970, 1186
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 1 cut(s) 167
BshFI GGCC 3 cut(s) 374, 1006, 1245
BshNI GGYRCC 1 cut(s) 191
BshTI ACCGGT 1 cut(s) 139
BsiSI CCGG 6 cut(s) 14, 33, 140, 189, 573, 1185
BslFI GGGAC 1 cut(s) 451
BslI CCNNNNNNNGG 2 cut(s) 1002, 1051
BsmAI GTCTC 1 cut(s) 1067
BsmFI GGGAC 1 cut(s) 451
BsnI GGCC 3 cut(s) 374, 1006, 1245
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 2 cut(s) 169, 779
BspANI GGCC 3 cut(s) 374, 1006, 1245
BspCNI CTCAG 2 cut(s) 742, 796
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 5 cut(s) 147, 171, 193, 230, 1056
BspMAI CTGCAG 1 cut(s) 775
BspMI ACCTGC 1 cut(s) 1042
BspOI GCTAGC 1 cut(s) 738
BspPI GGATC 2 cut(s) 164, 177
BspT107I GGYRCC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 564, 1147
BsrFI RCCGGY 2 cut(s) 139, 1184
BsrI ACTGG 3 cut(s) 296, 463, 936
BssAI RCCGGY 2 cut(s) 139, 1184
BssECI CCNNGG 5 cut(s) 150, 165, 224, 244, 1018
BssMI GATC 2 cut(s) 169, 779
BssNI GRCGYC 1 cut(s) 254
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 1020
Bst4CI ACNGT 5 cut(s) 361, 454, 637, 1135, 1165
BstACI GRCGYC 1 cut(s) 254
BstAPI GCANNNNNTGC 3 cut(s) 425, 663, 992
BstC8I GCNNGC 4 cut(s) 302, 306, 736, 1197
BstDEI CTNAG 2 cut(s) 729, 783
BstDSI CCRYGG 2 cut(s) 165, 224
BstFNI CGCG 1 cut(s) 167
BstKTI GATC 2 cut(s) 172, 782
BstMAI GTCTC 1 cut(s) 1067
BstMBI GATC 2 cut(s) 169, 779
BstMWI GCNNNNNNNGC 8 cut(s) 161, 173, 425, 527, 663, 980, 992, 1232
BstNI CCWGG 1 cut(s) 1020
BstPAI GACNNNNGTC 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 187, 571, 1018
BstSFI CTRYAG 1 cut(s) 771
BstSLI GKGCMC 1 cut(s) 366
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 3 cut(s) 757, 970, 1186
BstV2I GAAGAC 2 cut(s) 273, 1071
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 3 cut(s) 374, 1006, 1245
BtgI CCRYGG 2 cut(s) 165, 224
BveI ACCTGC 1 cut(s) 1042
Cac8I GCNNGC 4 cut(s) 302, 306, 736, 1197
CaiI CAGNNNCTG 1 cut(s) 469
Cfr10I RCCGGY 2 cut(s) 139, 1184
Cfr13I GGNCC 3 cut(s) 228, 1004, 1244
Cfr42I CCGCGG 1 cut(s) 168
CseI GACGC 1 cut(s) 262
Csp6I GTAC 2 cut(s) 498, 1131
CspAI ACCGGT 1 cut(s) 139
CviAII CATG 1 cut(s) 840
CviQI GTAC 2 cut(s) 498, 1131
DdeI CTNAG 2 cut(s) 729, 783
DpnI GATC 2 cut(s) 171, 781
DpnII GATC 2 cut(s) 169, 779
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
Eco130I CCWWGG 1 cut(s) 244
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 1244
EcoRII CCWGG 1 cut(s) 1018
EcoRV GATATC 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 1 cut(s) 843
FalI AAGNNNNNCTT 4 cut(s) 810, 842, 905, 937
FaqI GGGAC 1 cut(s) 451
FatI CATG 1 cut(s) 839
FauI CCCGC 4 cut(s) 192, 523, 615, 749
FauNDI CATATG 1 cut(s) 662
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 6 cut(s) 162, 165, 771, 984, 1049, 1200
Fsp4HI GCNGC 6 cut(s) 162, 165, 771, 984, 1049, 1200
FspBI CTAG 6 cut(s) 501, 735, 749, 896, 1113, 1239
GluI GCNGC 6 cut(s) 162, 165, 771, 984, 1049, 1200
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 3 cut(s) 374, 1006, 1245
HapII CCGG 6 cut(s) 14, 33, 140, 189, 573, 1185
HgaI GACGC 1 cut(s) 262
Hin1I GRCGYC 1 cut(s) 254
Hin1II CATG 1 cut(s) 843
HindIII AAGCTT 2 cut(s) 327, 824
HinfI GANTC 4 cut(s) 17, 240, 641, 1028
HpaII CCGG 6 cut(s) 14, 33, 140, 189, 573, 1185
HphI GGTGA 2 cut(s) 173, 275
Hpy166II GTNNAC 4 cut(s) 38, 228, 883, 1131
Hpy188I TCNGA 4 cut(s) 239, 252, 1124, 1191
Hpy188III TCNNGA 2 cut(s) 749, 1098
Hpy8I GTNNAC 4 cut(s) 38, 228, 883, 1131
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 5 cut(s) 361, 454, 637, 1135, 1165
HpyCH4IV ACGT 2 cut(s) 885, 1127
HpyF10VI GCNNNNNNNGC 8 cut(s) 161, 173, 425, 527, 663, 980, 992, 1232
HpyF3I CTNAG 2 cut(s) 729, 783
HpySE526I ACGT 2 cut(s) 885, 1127
Hsp92I GRCGYC 1 cut(s) 254
Hsp92II CATG 1 cut(s) 843
KspI CCGCGG 1 cut(s) 168
Kzo9I GATC 2 cut(s) 169, 779
LmnI GCTCC 4 cut(s) 925, 979, 1022, 1103
Lsp1109I GCAGC 3 cut(s) 757, 970, 1186
LweI GCATC 1 cut(s) 712
MaeI CTAG 6 cut(s) 501, 735, 749, 896, 1113, 1239
MaeII ACGT 2 cut(s) 885, 1127
MaeIII GTNAC 3 cut(s) 179, 577, 866
MalI GATC 2 cut(s) 171, 781
MboI GATC 2 cut(s) 169, 779
MboII GAAGA 6 cut(s) 278, 559, 698, 814, 1076, 1093
MfeI CAATTG 1 cut(s) 862
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 9 cut(s) 76, 90, 120, 208, 379, 510, 593, 862, 999
MlyI GAGTC 1 cut(s) 1037
MmeI TCCRAC 3 cut(s) 217, 380, 880
MroXI GAANNNNTTC 1 cut(s) 315
MseI TTAA 2 cut(s) 740, 1209
MslI CAYNNNNRTG 1 cut(s) 708
MspA1I CMGCKG 2 cut(s) 167, 983
MspI CCGG 6 cut(s) 14, 33, 140, 189, 573, 1185
MspR9I CCNGG 3 cut(s) 189, 573, 1020
MunI CAATTG 1 cut(s) 862
MvaI CCWGG 1 cut(s) 1020
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 8 cut(s) 161, 173, 425, 527, 663, 980, 992, 1232
NciI CCSGG 2 cut(s) 189, 573
NdeI CATATG 1 cut(s) 662
NdeII GATC 2 cut(s) 169, 779
NheI GCTAGC 1 cut(s) 734
NlaIII CATG 1 cut(s) 843
NlaIV GGNNCC 5 cut(s) 147, 171, 193, 230, 1056
NmeAIII GCCGAG 1 cut(s) 131
NmuCI GTSAC 2 cut(s) 179, 577
PcsI WCGNNNNNNNCGW 2 cut(s) 383, 882
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 3 cut(s) 17, 240, 641
PinAI ACCGGT 1 cut(s) 139
PkrI GCNGC 6 cut(s) 163, 166, 772, 985, 1050, 1201
PleI GAGTC 1 cut(s) 1036
PpsI GAGTC 1 cut(s) 1036
PshAI GACNNNNGTC 1 cut(s) 233
PsiI TTATAA 1 cut(s) 696
Psp6I CCWGG 1 cut(s) 1018
PspGI CCWGG 1 cut(s) 1018
PspN4I GGNNCC 5 cut(s) 147, 171, 193, 230, 1056
PspPI GGNCC 3 cut(s) 228, 1004, 1244
PstI CTGCAG 1 cut(s) 775
PstNI CAGNNNCTG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 983
RsaI GTAC 2 cut(s) 499, 1132
RsaNI GTAC 2 cut(s) 498, 1131
RseI CAYNNNNRTG 1 cut(s) 708
SacII CCGCGG 1 cut(s) 168
SaqAI TTAA 2 cut(s) 740, 1209
SatI GCNGC 6 cut(s) 162, 165, 771, 984, 1049, 1200
Sau3AI GATC 2 cut(s) 169, 779
Sau96I GGNCC 3 cut(s) 228, 1004, 1244
SchI GAGTC 1 cut(s) 1037
ScrFI CCNGG 3 cut(s) 189, 573, 1020
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 1 cut(s) 712
SfcI CTRYAG 1 cut(s) 771
Sfr303I CCGCGG 1 cut(s) 168
SgrBI CCGCGG 1 cut(s) 168
SinI GGWCC 1 cut(s) 228
SmiMI CAYNNNNRTG 1 cut(s) 708
Sse9I AATT 9 cut(s) 76, 90, 120, 208, 379, 510, 593, 862, 999
SspI AATATT 1 cut(s) 68
SspMI CTAG 6 cut(s) 501, 735, 749, 896, 1113, 1239
StyD4I CCNGG 3 cut(s) 187, 571, 1018
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 5 cut(s) 361, 454, 637, 1135, 1165
TaiI ACGT 2 cut(s) 888, 1130
TaqI TCGA 4 cut(s) 319, 377, 778, 962
TasI AATT 9 cut(s) 76, 90, 120, 208, 379, 510, 593, 862, 999
TatI WGTACW 2 cut(s) 497, 1130
TauI GCSGC 3 cut(s) 164, 167, 1051
TfiI GAWTC 3 cut(s) 17, 240, 641
Tru1I TTAA 2 cut(s) 740, 1209
Tru9I TTAA 2 cut(s) 740, 1209
TseFI GTSAC 2 cut(s) 179, 577
TseI GCWGC 3 cut(s) 770, 983, 1199
Tsp45I GTSAC 2 cut(s) 179, 577
TspDTI ATGAA 2 cut(s) 339, 449
TspGWI ACGGA 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 228
XapI RAATTY 1 cut(s) 90
XbaI TCTAGA 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 882
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 6 cut(s) 501, 735, 749, 896, 1113, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.