Rroxscaffold_3G00271120

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010569
Physical Location & Seq
Forward (+)
63527472 .. 63529700
2229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271120.1

Sequence Viewer

Length: 981 bp
ATGTTCAATTCTTCACAACATCCCGCCAATTTCGCATATCAATATCCCAACACCACCTTCGACCCCAACACTCAATTCTCCTATTTTCCTCCGGTAGCGTCCGATCACCCACCCGGAGTCGACCCGGGTCCCAATTCAGGCCCATACCCATTGACCCATGTTGGCCTCCAAAGTCACCTGCCATTTGGTGAAGACCCAAATGCTCCTTCACATGGTTGGCTTGTCAATCAAGCTGTCCCCATCTCATATGACGCTTCAATCAAATCTTTGAATGAGAGCTTGTTAGTGCCCACAAATGCAAATTCTTGGTGGAATAGCAGTTGGACAGATCAACCTAATGCGTTTCCAGGAACGTCCAATGGTATAGACAATGCCAGTCTGCCTAGCGAAATAAGCGGCGCGGGCGGGCAAGTGATGTGTGCGAGTACAAGTGCAGCTGCTGGTGACGAGTTGGGAACAAAGAGAAGGAAGGTTGACGCAGCGGTTGGTTTTGTGAAGATTTGCGAAATGTGCAATGTGACTACTCATAGCCCAGACGTTTACATGAAGCATTTGGCTGGGAAAAGGCACGCTGCTCAGGCTAACTTAATGCATCGAAGGGCTGGGTTGAATTCTGCTCCCACAATCCAATCTAAGTCCAATGGCATTTGGAAGAAAGACCCAAATAAAGTCAAGCTTGTTCAGTGTGTATGGTGTGAGTTTTGCAAGATCTACTGTAACAGCCAGGACGTCTATGTCAAACACATAGCTGGAAGGAAACATCAGAAGAACTTGGATCAACTCGAAAAGTTGAAGAACGAAGGTACTGCCCCGACCACAAATGTTCCATCAGGTGCAAGAAATCCACAAATTGGTCCCATGGAAAAGAATTCCCAGTCAAAGGCTCCTTCACAAGAGGATTTGGAGACCAAAAAGCTGAAGGTTATAAAGAGGGAGGAGCAGCAGCTGGGGAAGTTAGAACGTGCAATATTTGCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-met

Protein Analysis

326

Amino Acids

35.64

Weight (kDa)

8.72

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 231 - 254 3.6e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 926
AarI CACCTGC 1 cut(s) 186
AasI GACNNNNNNGTC 1 cut(s) 734
AatII GACGTC 1 cut(s) 732
Acc36I ACCTGC 1 cut(s) 186
AccB7I CCANNNNNTGG 1 cut(s) 851
AccI GTMKAC 1 cut(s) 120
AccII CGCG 1 cut(s) 401
AciI CCGC 5 cut(s) 24, 396, 401, 405, 482
AclWI GGATC 1 cut(s) 783
AcsI RAATTY 3 cut(s) 301, 610, 868
AcuI CTGAAG 1 cut(s) 938
AcyI GRCGYC 1 cut(s) 729
AfaI GTAC 2 cut(s) 427, 805
AfiI CCNNNNNNNGG 4 cut(s) 137, 212, 851, 880
AgsI TTSAA 5 cut(s) 7, 258, 271, 610, 793
AjnI CCWGG 2 cut(s) 346, 723
AjuI GAANNNNNNNTTGG 6 cut(s) 41, 59, 73, 91, 190, 222
AluBI AGCT 7 cut(s) 233, 279, 437, 676, 749, 916, 946
AluI AGCT 7 cut(s) 233, 279, 437, 676, 749, 916, 946
Alw26I GTCTC 1 cut(s) 899
AlwI GGATC 1 cut(s) 783
AlwNI CAGNNNCTG 2 cut(s) 440, 946
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 2 cut(s) 139, 163
ApeKI GCWGC 6 cut(s) 434, 437, 479, 572, 940, 943
ApoI RAATTY 3 cut(s) 301, 610, 868
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 3 cut(s) 128, 140, 854
AsuC2I CCSGG 3 cut(s) 114, 125, 126
AsuHPI GGTGA 4 cut(s) 98, 167, 200, 455
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 2 cut(s) 128, 854
BaeGI GKGCMC 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 6 cut(s) 424, 446, 491, 559, 952, 955
BccI CCATC 2 cut(s) 248, 835
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 2 cut(s) 348, 725
BcnI CCSGG 3 cut(s) 114, 125, 126
BcoDI GTCTC 1 cut(s) 899
BfaI CTAG 1 cut(s) 384
BfuAI ACCTGC 1 cut(s) 186
BglII AGATCT 1 cut(s) 708
BisI GCNGC 7 cut(s) 397, 435, 438, 480, 573, 941, 944
BlsI GCNGC 7 cut(s) 398, 436, 439, 481, 574, 942, 945
Bme1390I CCNGG 5 cut(s) 114, 125, 126, 348, 725
Bme18I GGWCC 2 cut(s) 128, 854
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 3 cut(s) 128, 140, 854
BmiI GGNNCC 4 cut(s) 129, 130, 856, 885
BmrFI CCNGG 5 cut(s) 114, 125, 126, 348, 725
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 1 cut(s) 601
BmuI ACTGGG 1 cut(s) 868
BoxI GACNNNNGTC 1 cut(s) 126
BpiI GAAGAC 1 cut(s) 198
Bpu10I CCTNAGC 1 cut(s) 576
BpuMI CCSGG 3 cut(s) 114, 125, 126
BsaHI GRCGYC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 2 cut(s) 124, 858
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 212, 851, 880
Bse1I ACTGG 2 cut(s) 375, 874
Bse3DI GCAATG 2 cut(s) 520, 981
BseBI CCWGG 2 cut(s) 348, 725
BseDI CCNNGG 2 cut(s) 124, 858
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 4 cut(s) 137, 212, 851, 880
BseMI GCAATG 2 cut(s) 520, 981
BseMII CTCAG 1 cut(s) 590
BseNI ACTGG 2 cut(s) 375, 874
BseRI GAGGAG 1 cut(s) 950
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 6 cut(s) 424, 446, 491, 559, 952, 955
BseYI CCCAGC 3 cut(s) 557, 602, 946
BsgI GTGCAG 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 401
BshFI GGCC 2 cut(s) 141, 165
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 3 cut(s) 92, 114, 125
BslFI GGGAC 3 cut(s) 114, 221, 840
BslI CCNNNNNNNGG 4 cut(s) 137, 212, 851, 880
BsmAI GTCTC 1 cut(s) 899
BsmFI GGGAC 3 cut(s) 114, 221, 840
BsnI GGCC 2 cut(s) 141, 165
Bso31I GGTCTC 1 cut(s) 899
BsoBI CYCGRG 1 cut(s) 124
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 4 cut(s) 103, 328, 708, 775
Bsp19I CCATGG 1 cut(s) 858
BspACI CCGC 5 cut(s) 24, 396, 401, 405, 482
BspANI GGCC 2 cut(s) 141, 165
BspCNI CTCAG 1 cut(s) 589
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 4 cut(s) 129, 130, 856, 885
BspMI ACCTGC 1 cut(s) 186
BspPI GGATC 1 cut(s) 783
BspTNI GGTCTC 1 cut(s) 899
BsrDI GCAATG 2 cut(s) 520, 981
BsrI ACTGG 2 cut(s) 375, 874
BssECI CCNNGG 2 cut(s) 124, 858
BssMI GATC 4 cut(s) 103, 328, 708, 775
BssNI GRCGYC 1 cut(s) 729
BssT1I CCWWGG 1 cut(s) 858
Bst2UI CCWGG 2 cut(s) 348, 725
Bst4CI ACNGT 1 cut(s) 716
BstACI GRCGYC 1 cut(s) 729
BstAPI GCANNNNNTGC 1 cut(s) 971
BstC8I GCNNGC 3 cut(s) 403, 407, 570
BstDEI CTNAG 2 cut(s) 576, 633
BstDSI CCRYGG 1 cut(s) 858
BstF5I GGATG 1 cut(s) 19
BstFNI CGCG 1 cut(s) 401
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 4 cut(s) 106, 331, 711, 778
BstMAI GTCTC 1 cut(s) 899
BstMBI GATC 4 cut(s) 103, 328, 708, 775
BstMWI GCNNNNNNNGC 6 cut(s) 32, 393, 402, 510, 578, 971
BstNI CCWGG 2 cut(s) 348, 725
BstPAI GACNNNNGTC 1 cut(s) 126
BstSCI CCNGG 5 cut(s) 112, 123, 124, 346, 723
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 6 cut(s) 424, 446, 491, 559, 952, 955
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 708
BstYI RGATCY 1 cut(s) 708
BsuRI GGCC 2 cut(s) 141, 165
BtgI CCRYGG 1 cut(s) 858
BtsCI GGATG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 689
BveI ACCTGC 1 cut(s) 186
Cac8I GCNNGC 3 cut(s) 403, 407, 570
CaiI CAGNNNCTG 2 cut(s) 440, 946
CfoI GCGC 1 cut(s) 401
Cfr13I GGNCC 3 cut(s) 128, 140, 854
Cfr9I CCCGGG 1 cut(s) 124
CseI GACGC 3 cut(s) 87, 260, 485
Csp6I GTAC 2 cut(s) 426, 804
CviAII CATG 4 cut(s) 158, 212, 544, 859
CviQI GTAC 2 cut(s) 426, 804
DdeI CTNAG 2 cut(s) 576, 633
DpnI GATC 4 cut(s) 105, 330, 710, 777
DpnII GATC 4 cut(s) 103, 328, 708, 775
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
Eco130I CCWWGG 1 cut(s) 858
Eco31I GGTCTC 1 cut(s) 899
Eco47I GGWCC 2 cut(s) 128, 854
Eco57I CTGAAG 1 cut(s) 938
Eco88I CYCGRG 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 128
EcoRI GAATTC 2 cut(s) 610, 868
EcoRII CCWGG 2 cut(s) 346, 723
EcoT14I CCWWGG 1 cut(s) 858
EcoT22I ATGCAT 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 858
FaeI CATG 4 cut(s) 161, 215, 547, 862
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 3 cut(s) 114, 221, 840
FatI CATG 4 cut(s) 157, 211, 543, 858
FauI CCCGC 3 cut(s) 31, 394, 398
FauNDI CATATG 1 cut(s) 247
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 7 cut(s) 397, 435, 438, 480, 573, 941, 944
FokI GGATG 1 cut(s) 6
Fsp4HI GCNGC 7 cut(s) 397, 435, 438, 480, 573, 941, 944
FspBI CTAG 1 cut(s) 384
GlaI GCGC 1 cut(s) 400
GluI GCNGC 7 cut(s) 397, 435, 438, 480, 573, 941, 944
GsaI CCCAGC 3 cut(s) 561, 606, 950
HaeIII GGCC 2 cut(s) 141, 165
HapII CCGG 3 cut(s) 92, 114, 125
HgaI GACGC 3 cut(s) 87, 260, 485
HhaI GCGC 1 cut(s) 401
Hin1I GRCGYC 1 cut(s) 729
Hin1II CATG 4 cut(s) 161, 215, 547, 862
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HincII GTYRAC 2 cut(s) 121, 475
HindII GTYRAC 2 cut(s) 121, 475
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 1 cut(s) 117
HpaII CCGG 3 cut(s) 92, 114, 125
HphI GGTGA 4 cut(s) 98, 167, 200, 455
Hpy166II GTNNAC 3 cut(s) 121, 475, 541
Hpy188I TCNGA 2 cut(s) 103, 765
Hpy8I GTNNAC 3 cut(s) 121, 475, 541
HpyAV CCTTC 9 cut(s) 67, 216, 459, 463, 591, 747, 794, 897, 913
HpyCH4III ACNGT 1 cut(s) 716
HpyCH4IV ACGT 4 cut(s) 353, 537, 729, 961
HpyCH4V TGCA 8 cut(s) 299, 434, 513, 592, 705, 836, 965, 974
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 393, 402, 510, 578, 971
HpyF3I CTNAG 2 cut(s) 576, 633
HpySE526I ACGT 4 cut(s) 353, 537, 729, 961
Hsp92I GRCGYC 1 cut(s) 729
Hsp92II CATG 4 cut(s) 161, 215, 547, 862
HspAI GCGC 1 cut(s) 399
KflI GGGWCCC 1 cut(s) 128
Kzo9I GATC 4 cut(s) 103, 328, 708, 775
LmnI GCTCC 4 cut(s) 208, 622, 889, 937
Lsp1109I GCAGC 6 cut(s) 424, 446, 491, 559, 952, 955
LweI GCATC 1 cut(s) 601
MaeI CTAG 1 cut(s) 384
MaeII ACGT 4 cut(s) 353, 537, 729, 961
MaeIII GTNAC 4 cut(s) 173, 443, 517, 716
MalI GATC 4 cut(s) 105, 330, 710, 777
MboI GATC 4 cut(s) 103, 328, 708, 775
MboII GAAGA 6 cut(s) 3, 203, 508, 664, 778, 805
MflI RGATCY 1 cut(s) 708
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 8 cut(s) 7, 28, 74, 133, 301, 610, 849, 868
MlyI GAGTC 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 5 cut(s) 99, 176, 889, 924, 928
Mph1103I ATGCAT 1 cut(s) 594
MseI TTAA 1 cut(s) 587
MspA1I CMGCKG 3 cut(s) 437, 482, 946
MspI CCGG 3 cut(s) 92, 114, 125
MspR9I CCNGG 5 cut(s) 114, 125, 126, 348, 725
MvaI CCWGG 2 cut(s) 348, 725
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 6 cut(s) 32, 393, 402, 510, 578, 971
NciI CCSGG 3 cut(s) 114, 125, 126
NcoI CCATGG 1 cut(s) 858
NdeI CATATG 1 cut(s) 247
NdeII GATC 4 cut(s) 103, 328, 708, 775
NlaIII CATG 4 cut(s) 161, 215, 547, 862
NlaIV GGNNCC 4 cut(s) 129, 130, 856, 885
NmuCI GTSAC 3 cut(s) 173, 443, 517
NsiI ATGCAT 1 cut(s) 594
PaqCI CACCTGC 1 cut(s) 186
PflMI CCANNNNNTGG 1 cut(s) 851
PfoI TCCNGGA 1 cut(s) 346
PkrI GCNGC 7 cut(s) 398, 436, 439, 481, 574, 942, 945
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 126
PsiI TTATAA 1 cut(s) 926
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 2 cut(s) 346, 723
PspFI CCCAGC 3 cut(s) 557, 602, 946
PspGI CCWGG 2 cut(s) 346, 723
PspN4I GGNNCC 4 cut(s) 129, 130, 856, 885
PspPI GGNCC 3 cut(s) 128, 140, 854
PspPPI RGGWCCY 1 cut(s) 128
PstNI CAGNNNCTG 2 cut(s) 440, 946
PsuI RGATCY 1 cut(s) 708
PvuII CAGCTG 2 cut(s) 437, 946
RsaI GTAC 2 cut(s) 427, 805
RsaNI GTAC 2 cut(s) 426, 804
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 7 cut(s) 397, 435, 438, 480, 573, 941, 944
Sau3AI GATC 4 cut(s) 103, 328, 708, 775
Sau96I GGNCC 3 cut(s) 128, 140, 854
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 5 cut(s) 114, 125, 126, 348, 725
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 1 cut(s) 601
SinI GGWCC 2 cut(s) 128, 854
SmaI CCCGGG 1 cut(s) 126
Sse9I AATT 8 cut(s) 7, 28, 74, 133, 301, 610, 849, 868
SsiI CCGC 5 cut(s) 24, 396, 401, 405, 482
SspI AATATT 1 cut(s) 969
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 5 cut(s) 112, 123, 124, 346, 723
StyI CCWWGG 1 cut(s) 858
TaaI ACNGT 1 cut(s) 716
TaiI ACGT 4 cut(s) 356, 540, 732, 964
TaqI TCGA 4 cut(s) 60, 120, 595, 783
TasI AATT 8 cut(s) 7, 28, 74, 133, 301, 610, 849, 868
TatI WGTACW 1 cut(s) 425
TauI GCSGC 1 cut(s) 399
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TscAI CASTG 1 cut(s) 689
TseFI GTSAC 3 cut(s) 173, 443, 517
TseI GCWGC 6 cut(s) 434, 437, 479, 572, 940, 943
Tsp45I GTSAC 3 cut(s) 173, 443, 517
TspDTI ATGAA 1 cut(s) 560
TspMI CCCGGG 1 cut(s) 124
TspRI CASTG 1 cut(s) 689
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 2 cut(s) 128, 854
XapI RAATTY 3 cut(s) 301, 610, 868
XmaI CCCGGG 1 cut(s) 124
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 1 cut(s) 384
ZraI GACGTC 1 cut(s) 730
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.