FvH4_5g15440

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013677
Physical Location & Seq
Forward (+)
8736563 .. 8737635
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15440.t1

Sequence Viewer

Length: 744 bp
ATGGCAAATGTTAGAGGAAGAACAGCAGGGATGCTCATGGGAGAGAAAGTGTCACCAATGACGCCCACGCGTGTGAAGATTGTCCCTGGTTCTGGCTGGACTGAGGACTCTAATGGCCATTATCTTCTTGTTGACCTGGAAGGTTTCAACAAGGAGAAGGTGAGGCTGGAGGTGAACACCACAGCTGGACATCTAACAGTCACCGGAGACAGGCAAGTGAACGAGAGGGAACACGACTATATTGATGAAAATTTTCCGCTACCAGCAAACTCAGATGTCAATAAGATCACTGGGAAGTTTGATGGTGAGATTCTCTATGTAACTGTACCAAAGGTTGTGGCAGTGGTAGAACCAAAGAAGGATCCTGAAATAGCAGAAGCAGTGAAGGAGCCTGAAATAGTAGAAGCAAGGAAGGAGCCTCAAATAGTAGAACCAAAGAAGGAGCCTCAAATAGTTCAAGAAAGGAAAAAACCTGAAATAACGGAGAATCAGAAGATTGAAGCTGCTCCAGCAGCAGAATACACAAACAAATATGATGGCACAGATGATGCAGAGCCAAACAGATTATTAGGCAATGCTTGGATATTCTTTTCTGCAGAGAATATAAAGAAGTGGGAGGAAGATAATGGCATTTTAAGAAGTGCAAGGGAGGTGTTGAGCAAAAACAAAGGGATAGTTATCACAGCTATAGTAGCATTCTCGTTAGGGATGTTTGTTTCTCACAAGTTCGAAACAGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

248

Amino Acids

27.59

Weight (kDa)

5.2

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 117 8.3e-08 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 70
AciI CCGC 1 cut(s) 257
AclWI GGATC 2 cut(s) 356, 369
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 250
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 1 cut(s) 327
AfiI CCNNNNNNNGG 2 cut(s) 92, 210
AflIII ACRYGT 1 cut(s) 68
AgsI TTSAA 3 cut(s) 148, 458, 500
AjnI CCWGG 2 cut(s) 85, 135
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 3 cut(s) 185, 503, 686
AluI AGCT 3 cut(s) 185, 503, 686
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 2 cut(s) 356, 369
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 503, 512
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 1 cut(s) 252
AsuHPI GGTGA 5 cut(s) 45, 172, 184, 193, 317
AsuII TTCGAA 1 cut(s) 729
BalI TGGCCA 1 cut(s) 117
BamHI GGATCC 1 cut(s) 361
BbvI GCAGC 2 cut(s) 490, 524
BccI CCATC 2 cut(s) 296, 530
BciT130I CCWGG 2 cut(s) 87, 137
BcoDI GTCTC 1 cut(s) 201
BfmI CTRYAG 2 cut(s) 594, 687
BisI GCNGC 2 cut(s) 504, 513
BlsI GCNGC 2 cut(s) 505, 514
Bme1390I CCNGG 2 cut(s) 87, 137
BmiI GGNNCC 4 cut(s) 363, 390, 417, 444
BmrFI CCNGG 2 cut(s) 87, 137
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 2 cut(s) 21, 538
BmuI ACTGGG 1 cut(s) 300
BpmI CTGGAG 2 cut(s) 188, 492
Bpu14I TTCGAA 1 cut(s) 729
BsaBI GATNNNNATC 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 85
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 210
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 1 cut(s) 580
Bse8I GATNNNNATC 1 cut(s) 677
BseBI CCWGG 2 cut(s) 87, 137
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 2 cut(s) 36, 714
BseJI GATNNNNATC 1 cut(s) 677
BseLI CCNNNNNNNGG 2 cut(s) 92, 210
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 2 cut(s) 93, 285
BseNI ACTGG 1 cut(s) 295
BseXI GCAGC 2 cut(s) 490, 524
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 92, 210
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 695
BsnI GGCC 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 729
Bsp143I GATC 2 cut(s) 285, 361
BspACI CCGC 1 cut(s) 257
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 94, 284
BspFNI CGCG 1 cut(s) 70
BspLI GGNNCC 4 cut(s) 363, 390, 417, 444
BspMAI CTGCAG 1 cut(s) 598
BspPI GGATC 2 cut(s) 356, 369
BspT104I TTCGAA 1 cut(s) 729
BsrDI GCAATG 1 cut(s) 580
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 2 cut(s) 285, 361
BssNI GRCGYC 1 cut(s) 62
Bst2UI CCWGG 2 cut(s) 87, 137
Bst4CI ACNGT 2 cut(s) 199, 325
BstACI GRCGYC 1 cut(s) 62
BstBI TTCGAA 1 cut(s) 729
BstDEI CTNAG 2 cut(s) 102, 271
BstF5I GGATG 2 cut(s) 36, 714
BstFNI CGCG 1 cut(s) 70
BstKTI GATC 2 cut(s) 288, 364
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 2 cut(s) 285, 361
BstMWI GCNNNNNNNGC 3 cut(s) 509, 512, 692
BstNI CCWGG 2 cut(s) 87, 137
BstSCI CCNGG 2 cut(s) 85, 135
BstSFI CTRYAG 2 cut(s) 594, 687
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 2 cut(s) 490, 524
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 2 cut(s) 36, 714
BtsI GCAGTG 2 cut(s) 348, 387
BtsIMutI CAGTG 3 cut(s) 288, 348, 387
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 326
CspCI CAANNNNNGTGG 2 cut(s) 318, 353
CviAII CATG 1 cut(s) 37
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 2 cut(s) 102, 271
DpnI GATC 2 cut(s) 287, 363
DpnII GATC 2 cut(s) 285, 361
EaeI YGGCCR 1 cut(s) 115
EcoRII CCWGG 2 cut(s) 85, 135
FaeI CATG 1 cut(s) 40
FaiI YATR 6 cut(s) 38, 240, 318, 534, 605, 689
FaqI GGGAC 1 cut(s) 68
FatI CATG 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 504, 513
FokI GGATG 2 cut(s) 43, 721
Fsp4HI GCNGC 2 cut(s) 504, 513
GluI GCNGC 2 cut(s) 504, 513
GsuI CTGGAG 2 cut(s) 188, 492
HaeIII GGCC 1 cut(s) 117
HapII CCGG 1 cut(s) 204
HgaI GACGC 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 1 cut(s) 40
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HinfI GANTC 3 cut(s) 107, 310, 487
HpaII CCGG 1 cut(s) 204
HphI GGTGA 5 cut(s) 45, 172, 184, 193, 317
Hpy166II GTNNAC 3 cut(s) 133, 175, 220
Hpy188I TCNGA 2 cut(s) 274, 492
Hpy188III TCNNGA 2 cut(s) 365, 458
Hpy8I GTNNAC 3 cut(s) 133, 175, 220
HpyAV CCTTC 6 cut(s) 134, 151, 352, 379, 406, 433
HpyCH4III ACNGT 2 cut(s) 199, 325
HpyCH4V TGCA 3 cut(s) 551, 596, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 512, 692
HpyF3I CTNAG 2 cut(s) 102, 271
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 1 cut(s) 40
Kzo9I GATC 2 cut(s) 285, 361
LmnI GCTCC 4 cut(s) 388, 415, 442, 511
Lsp1109I GCAGC 2 cut(s) 490, 524
LweI GCATC 2 cut(s) 21, 538
MaeIII GTNAC 3 cut(s) 51, 199, 319
MalI GATC 2 cut(s) 287, 363
MboI GATC 2 cut(s) 285, 361
MboII GAAGA 5 cut(s) 30, 88, 116, 505, 632
MflI RGATCY 1 cut(s) 361
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 1 cut(s) 250
MluI ACGCGT 1 cut(s) 68
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 9 cut(s) 8, 97, 156, 163, 219, 429, 456, 610, 643
Mox20I TGGCCA 1 cut(s) 117
MroXI GAANNNNTTC 1 cut(s) 252
MscI TGGCCA 1 cut(s) 117
MseI TTAA 1 cut(s) 635
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 2 cut(s) 87, 137
Mva1269I GAATGC 1 cut(s) 695
MvaI CCWGG 2 cut(s) 87, 137
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 3 cut(s) 509, 512, 692
NdeII GATC 2 cut(s) 285, 361
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 4 cut(s) 363, 390, 417, 444
NmuCI GTSAC 2 cut(s) 51, 199
NspV TTCGAA 1 cut(s) 729
OliI CACNNNNGTG 1 cut(s) 71
PctI GAATGC 1 cut(s) 695
PdmI GAANNNNTTC 1 cut(s) 252
PfeI GAWTC 2 cut(s) 310, 487
PkrI GCNGC 2 cut(s) 505, 514
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Psp6I CCWGG 2 cut(s) 85, 135
PspGI CCWGG 2 cut(s) 85, 135
PspN4I GGNNCC 4 cut(s) 363, 390, 417, 444
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 635
SatI GCNGC 2 cut(s) 504, 513
Sau3AI GATC 2 cut(s) 285, 361
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 87, 137
SfaNI GCATC 2 cut(s) 21, 538
SfcI CTRYAG 2 cut(s) 594, 687
SfuI TTCGAA 1 cut(s) 729
SmiMI CAYNNNNRTG 1 cut(s) 71
Sse9I AATT 1 cut(s) 250
SsiI CCGC 1 cut(s) 257
StyD4I CCNGG 2 cut(s) 85, 135
TaaI ACNGT 2 cut(s) 199, 325
TaqI TCGA 1 cut(s) 729
TasI AATT 1 cut(s) 250
TfiI GAWTC 2 cut(s) 310, 487
Tru1I TTAA 1 cut(s) 635
Tru9I TTAA 1 cut(s) 635
TscAI CASTG 3 cut(s) 295, 348, 387
TseFI GTSAC 2 cut(s) 51, 199
TseI GCWGC 2 cut(s) 503, 512
Tsp45I GTSAC 2 cut(s) 51, 199
TspDTI ATGAA 1 cut(s) 261
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 3 cut(s) 295, 348, 387
XapI RAATTY 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.