Prupe.5G186800_v2.0.a1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013677
Physical Location & Seq
Reverse (-)
15439722 .. 15441048
1327 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G186800.1

Sequence Viewer

Length: 702 bp
ATGGCACAAGTCAGAAGATCATCAACAAGAGGGATGGGAGAGAGATCGTCATCATCGACACGCTTGCACCAAAAGATTTTGCCTTCCTCAGGTTGGACAGAGGACTCCAATGGCCATTATCTTCTTGTTGATCTTCCAGATTTTAAGAAGGAGGAGGTGAAGCTTGTGGTGAATGTCTCAGCTGGCCATTTAACAGTCAGCGGACAAAGGCAAGTGAATGAGAAGAAATCTGAGTATTTTGAGCAGAACTTTACCATCCCTCCAAACTCAGATGTCGATAAGATCACCGGAAAATTTGACGGTGAGATTCTCTATGTCACTGTGCCAAAGGTAGTAGCAGTTGTAGAAGAAAAGATAGAAGCGGAACCTGAAATAGAAAACCAAAATGTTGATGAAACCGCTGCCACAGAACCAGCTAAGCCAGAGAACAAAAATGTGGAAGGCGCTGCAGCAGAAAACATACACTTAACAAGACCGAAAAATGATGGACAGCACTCGAACAAAGATGATGGAGCCTCAAAAAAAGTATCAGGAATTAATGACCGCATATTCTTTTCTCTGGAGAATATAAGGAAATGGGATGAGCATGAAGATGGGATTTTGAAGACTGCAATGGAGATGTTGAGCAAAAATAAAGGAGTTATAACAGCTGTGGTGGCATTTTCACTAGGGGTGCTAGTTGCTCGTCAGACGTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

234

Amino Acids

25.85

Weight (kDa)

5.61

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AciI CCGC 4 cut(s) 201, 362, 399, 544
AcoI YGGCCR 2 cut(s) 112, 184
AcsI RAATTY 1 cut(s) 293
AfiI CCNNNNNNNGG 2 cut(s) 89, 93
AgsI TTSAA 1 cut(s) 604
AluBI AGCT 4 cut(s) 163, 182, 416, 650
AluI AGCT 4 cut(s) 163, 182, 416, 650
Alw26I GTCTC 1 cut(s) 181
AoxI GGCC 2 cut(s) 112, 184
ApeKI GCWGC 3 cut(s) 401, 446, 449
ApoI RAATTY 1 cut(s) 293
AseI ATTAAT 1 cut(s) 537
AspLEI GCGC 1 cut(s) 446
AsuHPI GGTGA 4 cut(s) 169, 181, 277, 314
AxyI CCTNAGG 1 cut(s) 88
BalI TGGCCA 2 cut(s) 114, 186
BbsI GAAGAC 1 cut(s) 611
BbvI GCAGC 3 cut(s) 388, 433, 461
BccI CCATC 5 cut(s) 28, 263, 479, 503, 587
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 2 cut(s) 668, 677
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 447
BisI GCNGC 3 cut(s) 402, 447, 450
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 3 cut(s) 403, 448, 451
BmiI GGNNCC 2 cut(s) 366, 514
BpiI GAAGAC 1 cut(s) 611
BpmI CTGGAG 1 cut(s) 581
Bpu1102I GCTNAGC 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 49
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 93
Bse21I CCTNAGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 618
Bse8I GATNNNNATC 1 cut(s) 49
BseGI GGATG 3 cut(s) 39, 255, 586
BseJI GATNNNNATC 1 cut(s) 49
BseLI CCNNNNNNNGG 2 cut(s) 89, 93
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 4 cut(s) 102, 192, 222, 282
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 3 cut(s) 388, 433, 461
BshFI GGCC 2 cut(s) 114, 186
BsiSI CCGG 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 89, 93
BsmAI GTCTC 1 cut(s) 181
BsnI GGCC 2 cut(s) 114, 186
Bsp143I GATC 4 cut(s) 17, 44, 130, 282
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 4 cut(s) 201, 362, 399, 544
BspANI GGCC 2 cut(s) 114, 186
BspCNI CTCAG 4 cut(s) 101, 191, 223, 281
BspLI GGNNCC 2 cut(s) 366, 514
BspMAI CTGCAG 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 618
BssMI GATC 4 cut(s) 17, 44, 130, 282
Bst4CI ACNGT 3 cut(s) 196, 302, 322
BstC8I GCNNGC 2 cut(s) 65, 184
BstDEI CTNAG 5 cut(s) 88, 178, 231, 268, 417
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 3 cut(s) 39, 255, 586
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 4 cut(s) 20, 47, 133, 285
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 4 cut(s) 17, 44, 130, 282
BstMWI GCNNNNNNNGC 1 cut(s) 656
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 388, 433, 461
BstV2I GAAGAC 1 cut(s) 611
Bsu36I CCTNAGG 1 cut(s) 88
BsuRI GGCC 2 cut(s) 114, 186
BtsCI GGATG 3 cut(s) 39, 255, 586
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 2 cut(s) 65, 184
CfoI GCGC 1 cut(s) 446
CviAII CATG 1 cut(s) 587
CviJI RGCY 8 cut(s) 114, 163, 182, 186, 416, 421, 515, 650
CviKI_1 RGCY 8 cut(s) 114, 163, 182, 186, 416, 421, 515, 650
DdeI CTNAG 5 cut(s) 88, 178, 231, 268, 417
DpnI GATC 4 cut(s) 19, 46, 132, 284
DpnII GATC 4 cut(s) 17, 44, 130, 282
EaeI YGGCCR 2 cut(s) 112, 184
Eco81I CCTNAGG 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 87
FaeI CATG 1 cut(s) 590
FaiI YATR 6 cut(s) 315, 461, 548, 569, 588, 644
FatI CATG 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 402, 447, 450
FokI GGATG 3 cut(s) 46, 242, 593
Fsp4HI GCNGC 3 cut(s) 402, 447, 450
FspBI CTAG 2 cut(s) 668, 677
GlaI GCGC 1 cut(s) 445
GluI GCNGC 3 cut(s) 402, 447, 450
GsuI CTGGAG 1 cut(s) 581
HaeII RGCGCY 1 cut(s) 447
HaeIII GGCC 2 cut(s) 114, 186
HapII CCGG 1 cut(s) 288
HhaI GCGC 1 cut(s) 446
Hin1II CATG 1 cut(s) 590
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 2 cut(s) 104, 307
HpaII CCGG 1 cut(s) 288
HphI GGTGA 4 cut(s) 169, 181, 277, 314
Hpy188I TCNGA 4 cut(s) 14, 232, 271, 690
Hpy188III TCNNGA 3 cut(s) 137, 531, 560
HpyAV CCTTC 3 cut(s) 93, 142, 434
HpyCH4III ACNGT 3 cut(s) 196, 302, 322
HpyCH4IV ACGT 1 cut(s) 692
HpyCH4V TGCA 3 cut(s) 67, 449, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 656
HpyF3I CTNAG 5 cut(s) 88, 178, 231, 268, 417
HpySE526I ACGT 1 cut(s) 692
Hsp92II CATG 1 cut(s) 590
HspAI GCGC 1 cut(s) 444
Kzo9I GATC 4 cut(s) 17, 44, 130, 282
LmnI GCTCC 1 cut(s) 512
LpnPI CCDG 9 cut(s) 75, 150, 168, 301, 381, 426, 435, 516, 545
Lsp1109I GCAGC 3 cut(s) 388, 433, 461
MaeI CTAG 2 cut(s) 668, 677
MaeII ACGT 1 cut(s) 692
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 19, 46, 132, 284
MboI GATC 4 cut(s) 17, 44, 130, 282
MboII GAAGA 7 cut(s) 27, 113, 125, 235, 359, 602, 616
MlsI TGGCCA 2 cut(s) 114, 186
MluCI AATT 2 cut(s) 293, 534
MluNI TGGCCA 2 cut(s) 114, 186
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 7 cut(s) 23, 94, 97, 145, 148, 270, 526
Mox20I TGGCCA 2 cut(s) 114, 186
MscI TGGCCA 2 cut(s) 114, 186
MseI TTAA 4 cut(s) 144, 191, 467, 537
MslI CAYNNNNRTG 1 cut(s) 591
Msp20I TGGCCA 2 cut(s) 114, 186
MspA1I CMGCKG 4 cut(s) 182, 201, 401, 650
MspI CCGG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 656
NdeII GATC 4 cut(s) 17, 44, 130, 282
NlaIII CATG 1 cut(s) 590
NlaIV GGNNCC 2 cut(s) 366, 514
NmuCI GTSAC 1 cut(s) 316
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PfeI GAWTC 1 cut(s) 307
PkrI GCNGC 3 cut(s) 403, 448, 451
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PshBI ATTAAT 1 cut(s) 537
PsiI TTATAA 1 cut(s) 644
PspN4I GGNNCC 2 cut(s) 366, 514
PstI CTGCAG 1 cut(s) 451
PvuII CAGCTG 2 cut(s) 182, 650
RseI CAYNNNNRTG 1 cut(s) 591
SaqAI TTAA 4 cut(s) 144, 191, 467, 537
SatI GCNGC 3 cut(s) 402, 447, 450
Sau3AI GATC 4 cut(s) 17, 44, 130, 282
SchI GAGTC 1 cut(s) 98
SetI ASST 9 cut(s) 94, 159, 165, 184, 333, 370, 418, 652, 695
SfcI CTRYAG 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 591
Sse9I AATT 2 cut(s) 293, 534
SsiI CCGC 4 cut(s) 201, 362, 399, 544
SspMI CTAG 2 cut(s) 668, 677
TaaI ACNGT 3 cut(s) 196, 302, 322
TaiI ACGT 1 cut(s) 695
TaqI TCGA 3 cut(s) 56, 276, 497
TaqII GACCGA 1 cut(s) 490
TasI AATT 2 cut(s) 293, 534
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 4 cut(s) 144, 191, 467, 537
Tru9I TTAA 4 cut(s) 144, 191, 467, 537
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 3 cut(s) 401, 446, 449
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 408, 603
TspRI CASTG 1 cut(s) 325
VspI ATTAAT 1 cut(s) 537
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 1 cut(s) 293
XspI CTAG 2 cut(s) 668, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.