MD14G1195100.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013677
Physical Location & Seq
Reverse (-)
28594159 .. 28595302
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1195100.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGCAAATATCAGAGGATCAATGAGCGCTTTGGAAGAGAGATTACGGCCATCGACACCCACACGCATGCGCGAAAAGATTGTTCCTTCATCAGACTGGACGGACGACTCCAATTGTCATTACCTTCTCGTTGATCTTCCAGATTTTAAAAAGGAGGAGGTGAGGGTTGAGGTGAATATCTCAACCGGCCATATAACAATCAGCGGAAAAAGGCAAGTGAACGAAAGGATATCCCAGTATTTTGAGCAGATTATCACCCTTCCGCAACACTCAGATGTCGATAAGATCACCGGAAAATTTGACGGTGAGATTCTATATGTCACCGTACCAAAGGTGGCAGCAGCGGTTGAAGAACAGAAGAAAGAACCAGAAATAGAGAATGAAAATGTCCAAGGCAGCATCGCTGCTGCCACGGAACCTCCTCAAGTAGGGAAGAATGAAACTGTGGAAGGCTTAAGACCCAAAGATGAAGGAGTGCACTCAAAGAAAGCCGATGGCACAAAGTATGGAGAGATTAAAAAATTATTAGGAAATGAACACATATTTTTTTCTCCAGAAAATATAAAGAAATGGGAGGAGAAAGGTGGGAATATCGTAAGGAATGCAATGGAGATGTTGAGCAAAAACAAAGGGCTAGTTGTAACTGCTGTGGTTGCATTTTCATTAGGGATGCTAGTTAATCGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

230

Amino Acids

25.66

Weight (kDa)

6.26

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 122 6.9e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 72
AciI CCGC 3 cut(s) 204, 263, 344
AclWI GGATC 1 cut(s) 25
AcoI YGGCCR 2 cut(s) 47, 187
AcsI RAATTY 1 cut(s) 296
AfaI GTAC 1 cut(s) 327
AfeI AGCGCT 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 428
AflII CTTAAG 1 cut(s) 454
AgsI TTSAA 1 cut(s) 350
Alw21I GWGCWC 1 cut(s) 480
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 1 cut(s) 25
Aor51HI AGCGCT 1 cut(s) 28
AoxI GGCC 2 cut(s) 47, 187
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 5 cut(s) 338, 341, 396, 404, 407
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 2 cut(s) 29, 72
AsuHPI GGTGA 6 cut(s) 172, 184, 247, 280, 313, 317
BaeGI GKGCMC 1 cut(s) 480
BarI GAAGNNNNNNTAC 2 cut(s) 27, 59
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 5 cut(s) 350, 353, 391, 394, 408
BccI CCATC 2 cut(s) 58, 488
BceAI ACGGC 1 cut(s) 62
BfaI CTAG 2 cut(s) 635, 674
BfoI RGCGCY 1 cut(s) 30
BfrI CTTAAG 1 cut(s) 454
BisI GCNGC 5 cut(s) 339, 342, 397, 405, 408
BlsI GCNGC 5 cut(s) 340, 343, 398, 406, 409
BmiI GGNNCC 1 cut(s) 417
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 2 cut(s) 408, 660
BmuI ACTGGG 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 391, 411
BsaWI WCCGGW 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 101, 235
Bse3DI GCAATG 1 cut(s) 612
BseDI CCNNGG 2 cut(s) 391, 411
BseGI GGATG 1 cut(s) 675
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 101, 235
BseRI GAGGAG 3 cut(s) 170, 411, 590
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 5 cut(s) 350, 353, 391, 394, 408
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 49, 189
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 2 cut(s) 186, 291
BslI CCNNNNNNNGG 1 cut(s) 428
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 2 cut(s) 49, 189
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 3 cut(s) 17, 133, 285
BspACI CCGC 3 cut(s) 204, 263, 344
BspANI GGCC 2 cut(s) 49, 189
BspCNI CTCAG 1 cut(s) 284
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 417
BspPI GGATC 1 cut(s) 25
BspTI CTTAAG 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 101, 235
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 391, 411
BssMI GATC 3 cut(s) 17, 133, 285
BssT1I CCWWGG 1 cut(s) 391
Bst4CI ACNGT 3 cut(s) 305, 325, 445
Bst6I CTCTTC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 271
BstDSI CCRYGG 1 cut(s) 411
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 1 cut(s) 675
BstFNI CGCG 1 cut(s) 72
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 2 cut(s) 29, 72
BstKTI GATC 3 cut(s) 20, 136, 288
BstMBI GATC 3 cut(s) 17, 133, 285
BstMWI GCNNNNNNNGC 1 cut(s) 653
BstNSI RCATGY 1 cut(s) 70
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 5 cut(s) 350, 353, 391, 394, 408
BsuRI GGCC 2 cut(s) 49, 189
BtgI CCRYGG 1 cut(s) 411
BtgZI GCGATG 1 cut(s) 385
BtsCI GGATG 1 cut(s) 675
Cac8I GCNNGC 1 cut(s) 68
CfoI GCGC 2 cut(s) 29, 72
Cfr10I RCCGGY 1 cut(s) 185
Csp6I GTAC 1 cut(s) 326
CviAII CATG 1 cut(s) 67
CviJI RGCY 5 cut(s) 49, 189, 453, 491, 634
CviKI_1 RGCY 5 cut(s) 49, 189, 453, 491, 634
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 1 cut(s) 271
DpnI GATC 3 cut(s) 19, 135, 287
DpnII GATC 3 cut(s) 17, 133, 285
DraI TTTAAA 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 47, 187
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 391
Eco32I GATATC 1 cut(s) 231
Eco47III AGCGCT 1 cut(s) 28
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 1 cut(s) 70
FaiI YATR 8 cut(s) 68, 192, 194, 316, 318, 507, 542, 563
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 339, 342, 397, 405, 408
FokI GGATG 1 cut(s) 682
Fsp4HI GCNGC 5 cut(s) 339, 342, 397, 405, 408
FspBI CTAG 2 cut(s) 635, 674
GlaI GCGC 2 cut(s) 28, 71
GluI GCNGC 5 cut(s) 339, 342, 397, 405, 408
GsuI CTGGAG 1 cut(s) 537
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 49, 189
HapII CCGG 2 cut(s) 186, 291
HhaI GCGC 2 cut(s) 29, 72
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 2 cut(s) 27, 70
HinP1I GCGC 2 cut(s) 27, 70
HinfI GANTC 2 cut(s) 107, 310
HpaII CCGG 2 cut(s) 186, 291
HphI GGTGA 6 cut(s) 172, 184, 247, 280, 313, 317
Hpy166II GTNNAC 2 cut(s) 220, 478
Hpy188I TCNGA 3 cut(s) 14, 94, 274
Hpy188III TCNNGA 2 cut(s) 140, 554
Hpy8I GTNNAC 2 cut(s) 220, 478
HpyAV CCTTC 5 cut(s) 96, 134, 269, 443, 464
HpyCH4III ACNGT 3 cut(s) 305, 325, 445
HpyCH4V TGCA 3 cut(s) 478, 605, 656
HpyF10VI GCNNNNNNNGC 1 cut(s) 653
HpyF3I CTNAG 1 cut(s) 271
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 2 cut(s) 27, 70
Kzo9I GATC 3 cut(s) 17, 133, 285
LpnPI CCDG 7 cut(s) 82, 153, 199, 248, 304, 381, 567
Lsp1109I GCAGC 5 cut(s) 350, 353, 391, 394, 408
LweI GCATC 2 cut(s) 408, 660
MaeI CTAG 2 cut(s) 635, 674
MaeIII GTNAC 2 cut(s) 319, 640
MalI GATC 3 cut(s) 19, 135, 287
MboI GATC 3 cut(s) 17, 133, 285
MboII GAAGA 5 cut(s) 47, 128, 362, 370, 445
MfeI CAATTG 1 cut(s) 112
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 3 cut(s) 112, 296, 521
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 8 cut(s) 8, 148, 151, 156, 163, 429, 432, 568
MseI TTAA 4 cut(s) 147, 455, 516, 678
MslI CAYNNNNRTG 2 cut(s) 65, 273
MspA1I CMGCKG 2 cut(s) 204, 344
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 2 cut(s) 186, 291
MunI CAATTG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 607
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 653
NdeII GATC 3 cut(s) 17, 133, 285
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 1 cut(s) 70
PaeI GCATGC 1 cut(s) 70
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 1 cut(s) 310
PkrI GCNGC 5 cut(s) 340, 343, 398, 406, 409
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 417
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
RseI CAYNNNNRTG 2 cut(s) 65, 273
SaqAI TTAA 4 cut(s) 147, 455, 516, 678
SatI GCNGC 5 cut(s) 339, 342, 397, 405, 408
Sau3AI GATC 3 cut(s) 17, 133, 285
SchI GAGTC 1 cut(s) 101
SduI GDGCHC 1 cut(s) 480
SetI ASST 7 cut(s) 126, 162, 174, 336, 421, 586, 689
SfaNI GCATC 2 cut(s) 408, 660
SmiMI CAYNNNNRTG 2 cut(s) 65, 273
SmlI CTYRAG 2 cut(s) 423, 454
SmoI CTYRAG 2 cut(s) 423, 454
SphI GCATGC 1 cut(s) 70
Sse9I AATT 3 cut(s) 112, 296, 521
SsiI CCGC 3 cut(s) 204, 263, 344
SspMI CTAG 2 cut(s) 635, 674
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 3 cut(s) 305, 325, 445
TaqI TCGA 2 cut(s) 53, 279
TasI AATT 3 cut(s) 112, 296, 521
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 4 cut(s) 147, 455, 516, 678
Tru9I TTAA 4 cut(s) 147, 455, 516, 678
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 5 cut(s) 338, 341, 396, 404, 407
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 6 cut(s) 78, 396, 453, 483, 549, 651
TspGWI ACGGA 2 cut(s) 116, 428
Vha464I CTTAAG 1 cut(s) 454
VneI GTGCAC 1 cut(s) 476
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 70
XspI CTAG 2 cut(s) 635, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.