Rroxscaffold_3G00272710

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013677
Physical Location & Seq
Forward (+)
64728940 .. 64729833
894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272710.1

Sequence Viewer

Length: 894 bp
ATGTCAAATGTTAGAGGAAGAACATCAGGGATGCACAGGGGAGAGAGATTGTCAACATTCACGCCCACACGTGTGAAGATTGTTCCTGGTTCTGGCTGGACAGAGGACTCTAATGGCCATTATCTTCTTGTCGATCTGGAAGGTATTCATATTGTTTTTAATCCCCATTACATGCTTGATGACATGGAATCTGCCTTCTCACTTTCTTTGGCTCTTAAATGTGTTTTAGGTTTCAACAAGGAGAAGGTGAAGCTGGAGGTGAATACCACAGCTGGAGATCTAACAGTCACCGGAGAAAGGCAAATGAACGAGAGGGAACATGCCTATATTGAGGAAAATTTTCCAATACCAGAAAACTCAGATGTCGATAAGATCACCGGAAAGTTTGATGGTGAGATTCTCTATGTAACTGTACCAAAGGTTGTAGCATTAGTACAAGAAAACAAGGAACCTGAAATAGTAGAAGCAAAGGAGCCCGAAATAATAGAAGCAAAAAAGGAGCCTGAAATGGTAGAAGCAAAGATGGAGCCTGAAATAGTACAGCCAAAGAAGGAGCCTGAAATAGTTCAAGAAAAGAAAAGACCTGAAATAATAGAGAATCAGAAGGTCAAAGCTGCTCAAGCAGCAGAAAACACACACCTAAGAGCGCCCAAAAACAATGATGGAAAGTACAGAAACAAATATGCTGGCACAAATGATGCACAACCAAACAGATTATTAGGCAATGCTCGGATATACTTTTCTCCAGAGAATATACGGAAGTGGGAGGAAGATAAAGGCATTTTGAGAAGTGCAATGGAGGTGTTGAGCAAAAACAAAAGGATGGTTATAACAGCTATAGTAGCATTCTCGTTAGGGATGTTAGTATCTCAAAAGTTCAAAAGAGGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

297

Amino Acids

33.65

Weight (kDa)

6.17

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 75 - 146 6.1e-06 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 337
AcvI CACGTG 1 cut(s) 71
AfaI GTAC 4 cut(s) 414, 435, 540, 671
AfiI CCNNNNNNNGG 2 cut(s) 92, 297
AflIII ACRYGT 2 cut(s) 68, 70
AgsI TTSAA 3 cut(s) 235, 569, 880
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 4 cut(s) 253, 272, 614, 836
AluI AGCT 4 cut(s) 253, 272, 614, 836
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 614, 623
ApoI RAATTY 1 cut(s) 337
Asp700I GAANNNNTTC 3 cut(s) 144, 339, 564
AspLEI GCGC 1 cut(s) 649
AsuHPI GGTGA 5 cut(s) 259, 271, 280, 367, 404
BalI TGGCCA 1 cut(s) 117
BanII GRGCYC 1 cut(s) 477
BbrPI CACGTG 1 cut(s) 71
BbvI GCAGC 2 cut(s) 601, 635
BccI CCATC 4 cut(s) 383, 517, 656, 817
BciT130I CCWGG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 650
BglII AGATCT 1 cut(s) 277
BisI GCNGC 2 cut(s) 615, 624
BlsI GCNGC 2 cut(s) 616, 625
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 5 cut(s) 450, 474, 501, 528, 555
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 21, 688
BpmI CTGGAG 3 cut(s) 275, 294, 729
BpuEI CTTGAG 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 71
BsaWI WCCGGW 2 cut(s) 290, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 297
Bse3DI GCAATG 2 cut(s) 730, 801
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 3 cut(s) 36, 828, 864
BseLI CCNNNNNNNGG 2 cut(s) 92, 297
BseMI GCAATG 2 cut(s) 730, 801
BseMII CTCAG 1 cut(s) 372
BseXI GCAGC 2 cut(s) 601, 635
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 291, 378
BslI CCNNNNNNNGG 2 cut(s) 92, 297
BsmI GAATGC 1 cut(s) 845
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 477
Bsp143I GATC 3 cut(s) 133, 277, 372
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 371
BspLI GGNNCC 5 cut(s) 450, 474, 501, 528, 555
BsrDI GCAATG 2 cut(s) 730, 801
BssMI GATC 3 cut(s) 133, 277, 372
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 286, 412
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 688
BstDEI CTNAG 2 cut(s) 358, 641
BstF5I GGATG 3 cut(s) 36, 828, 864
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 3 cut(s) 136, 280, 375
BstMBI GATC 3 cut(s) 133, 277, 372
BstMWI GCNNNNNNNGC 3 cut(s) 620, 623, 842
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 2 cut(s) 175, 323
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 837
BstV1I GCAGC 2 cut(s) 601, 635
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 36, 828, 864
Cac8I GCNNGC 1 cut(s) 688
CfoI GCGC 1 cut(s) 649
Csp6I GTAC 4 cut(s) 413, 434, 539, 670
CviAII CATG 3 cut(s) 172, 184, 320
CviQI GTAC 4 cut(s) 413, 434, 539, 670
DdeI CTNAG 2 cut(s) 358, 641
DpnI GATC 3 cut(s) 135, 279, 374
DpnII GATC 3 cut(s) 133, 277, 372
EaeI YGGCCR 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 477
Eco72I CACGTG 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 477
FaeI CATG 3 cut(s) 175, 187, 323
FatI CATG 3 cut(s) 171, 183, 319
Fnu4HI GCNGC 2 cut(s) 615, 624
FokI GGATG 3 cut(s) 43, 835, 871
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 2 cut(s) 615, 624
GlaI GCGC 1 cut(s) 648
GluI GCNGC 2 cut(s) 615, 624
GsuI CTGGAG 3 cut(s) 275, 294, 729
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 291, 378
HhaI GCGC 1 cut(s) 649
Hin1II CATG 3 cut(s) 175, 187, 323
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 4 cut(s) 107, 188, 397, 598
HpaII CCGG 2 cut(s) 291, 378
HphI GGTGA 5 cut(s) 259, 271, 280, 367, 404
Hpy166II GTNNAC 1 cut(s) 54
Hpy188I TCNGA 3 cut(s) 361, 603, 732
Hpy188III TCNNGA 3 cut(s) 137, 569, 746
Hpy8I GTNNAC 1 cut(s) 54
HpyAV CCTTC 5 cut(s) 134, 205, 238, 544, 598
HpyCH4III ACNGT 2 cut(s) 286, 412
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 3 cut(s) 34, 701, 794
HpyF10VI GCNNNNNNNGC 3 cut(s) 620, 623, 842
HpyF3I CTNAG 2 cut(s) 358, 641
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 3 cut(s) 175, 187, 323
HspAI GCGC 1 cut(s) 647
Kzo9I GATC 3 cut(s) 133, 277, 372
LmnI GCTCC 4 cut(s) 472, 499, 526, 553
Lsp1109I GCAGC 2 cut(s) 601, 635
LweI GCATC 2 cut(s) 21, 688
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 2 cut(s) 286, 406
MalI GATC 3 cut(s) 135, 279, 374
MboI GATC 3 cut(s) 133, 277, 372
MboII GAAGA 4 cut(s) 30, 88, 116, 782
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 477
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 1 cut(s) 337
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 8 cut(s) 8, 97, 250, 306, 325, 760, 793, 878
Mox20I TGGCCA 1 cut(s) 117
MroXI GAANNNNTTC 3 cut(s) 144, 339, 564
MscI TGGCCA 1 cut(s) 117
MseI TTAA 2 cut(s) 159, 216
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 2 cut(s) 291, 378
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 845
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 620, 623, 842
NdeII GATC 3 cut(s) 133, 277, 372
NlaIII CATG 3 cut(s) 175, 187, 323
NlaIV GGNNCC 5 cut(s) 450, 474, 501, 528, 555
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 2 cut(s) 175, 323
OliI CACNNNNGTG 1 cut(s) 71
PctI GAATGC 1 cut(s) 845
PdmI GAANNNNTTC 3 cut(s) 144, 339, 564
PfeI GAWTC 3 cut(s) 188, 397, 598
PkrI GCNGC 2 cut(s) 616, 625
PleI GAGTC 1 cut(s) 101
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 71
PsiI TTATAA 1 cut(s) 830
Psp6I CCWGG 1 cut(s) 85
PspCI CACGTG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 5 cut(s) 450, 474, 501, 528, 555
PsuI RGATCY 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 4 cut(s) 414, 435, 540, 671
RsaNI GTAC 4 cut(s) 413, 434, 539, 670
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 2 cut(s) 159, 216
SatI GCNGC 2 cut(s) 615, 624
Sau3AI GATC 3 cut(s) 133, 277, 372
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 477
SfaNI GCATC 2 cut(s) 21, 688
SfcI CTRYAG 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 618
SmoI CTYRAG 1 cut(s) 618
Sse9I AATT 1 cut(s) 337
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 286, 412
TaiI ACGT 1 cut(s) 73
TaqI TCGA 2 cut(s) 132, 366
TasI AATT 1 cut(s) 337
TatI WGTACW 3 cut(s) 433, 538, 669
TfiI GAWTC 3 cut(s) 188, 397, 598
Tru1I TTAA 2 cut(s) 159, 216
Tru9I TTAA 2 cut(s) 159, 216
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 2 cut(s) 614, 623
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 2 cut(s) 137, 320
TspGWI ACGGA 1 cut(s) 772
XapI RAATTY 1 cut(s) 337
XceI RCATGY 2 cut(s) 175, 323
XmnI GAANNNNTTC 3 cut(s) 144, 339, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.