Rh7BG035700

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013677
Physical Location & Seq
Reverse (-)
2335421 .. 2336305
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG035700.1

Sequence Viewer

Length: 798 bp
ATGGCAAATGTTAGAGGAAGAACATCAGGGATGCACAGGGGAGAGAGATTGTCAACATTCACGCCCACACGTGTGAAGATTGTTCCTGGTTCTGGCTGGACAGAGGACTCTAATGGCCATTATCTTCTTGTCGATCTGGAAGGTTTCAACAAGGAGAAGGTGAAGCTGGAGGTGAATACCACAGCTGGACATCTAACAGTCACCGGAGAAAGGCAAGTGAACGAGAGGGAACACGTCTATATTGAGGAAAATTTTCCAATACCAGCAAACTCAGATGTCGATAAGATCACCGGAAAGTTTGATGGTGAGATTCTCTATGTAACTGTACCAAAGGTTGTAGCAGTAGTACAGGAACCTGAAATAGTAGAAGCAAAGGAGCCCGAAATAATAGAAGCAAAAAAGGAGCCTGAAATGGTAGAAGCAAAGATGGAGCCTGAAATAGTACAACCAAAGAAGGAGCCTGAAATAGTTCAAGAAAAGAAAAGACCTGAAATAATAGAGAATCAGAAGGTCGAAGCTGCTCCAGCAGCAGAAAACACACACCTAAGAGCGCCCAAAAACAATGATGGAAAGTACAGAAACAAATATGATGGCACAAATGATGCACAGCCAAACAGATTATTAGGCGATGCTCGGATATACTTTTCTCCAGAGAATATAAGGAAGTGGGAGGAAGATAAAGGCATTTTGAGAAGTGCAATGGAGGTGTTGAGCAAAAACAAAGGGATGGTCATAACAGCTATAGTGGCATTCTCGTTAGGGATATTAGTATCTCAAAAGTTCAAAACAGGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

265

Amino Acids

29.8

Weight (kDa)

5.75

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 119 1.7e-08 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 250
AcvI CACGTG 1 cut(s) 71
AfaI GTAC 4 cut(s) 327, 348, 444, 575
AfiI CCNNNNNNNGG 2 cut(s) 92, 210
AflIII ACRYGT 3 cut(s) 68, 70, 232
AgsI TTSAA 3 cut(s) 148, 473, 784
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 4 cut(s) 166, 185, 518, 740
AluI AGCT 4 cut(s) 166, 185, 518, 740
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 518, 527
ApoI RAATTY 1 cut(s) 250
ArsI GACNNNNNNTTYG 2 cut(s) 714, 746
Asp700I GAANNNNTTC 2 cut(s) 252, 468
AspLEI GCGC 1 cut(s) 553
AsuHPI GGTGA 5 cut(s) 172, 184, 193, 280, 317
BalI TGGCCA 1 cut(s) 117
BanII GRGCYC 1 cut(s) 381
BbrPI CACGTG 1 cut(s) 71
BbvI GCAGC 2 cut(s) 505, 539
BccI CCATC 5 cut(s) 296, 421, 560, 584, 721
BciT130I CCWGG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 741
BfoI RGCGCY 1 cut(s) 554
BisI GCNGC 2 cut(s) 519, 528
BlsI GCNGC 2 cut(s) 520, 529
Bme1390I CCNGG 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 235
BmiI GGNNCC 5 cut(s) 354, 378, 405, 432, 459
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 3 cut(s) 21, 592, 619
BpmI CTGGAG 3 cut(s) 188, 507, 633
BsaAI YACGTR 1 cut(s) 71
BsaWI WCCGGW 2 cut(s) 203, 290
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 210
Bse3DI GCAATG 1 cut(s) 705
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 36, 732
BseLI CCNNNNNNNGG 2 cut(s) 92, 210
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 505, 539
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 204, 291
BslI CCNNNNNNNGG 2 cut(s) 92, 210
BsmI GAATGC 1 cut(s) 749
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 133, 285
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 284
BspLI GGNNCC 5 cut(s) 354, 378, 405, 432, 459
BsrDI GCAATG 1 cut(s) 705
BssMI GATC 2 cut(s) 133, 285
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 199, 325
BstBAI YACGTR 1 cut(s) 71
BstDEI CTNAG 2 cut(s) 271, 545
BstF5I GGATG 2 cut(s) 36, 732
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 2 cut(s) 136, 288
BstMBI GATC 2 cut(s) 133, 285
BstMWI GCNNNNNNNGC 3 cut(s) 524, 527, 746
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 741
BstV1I GCAGC 2 cut(s) 505, 539
BsuRI GGCC 1 cut(s) 117
BtgZI GCGATG 1 cut(s) 642
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 2 cut(s) 36, 732
CfoI GCGC 1 cut(s) 553
Csp6I GTAC 4 cut(s) 326, 347, 443, 574
CviQI GTAC 4 cut(s) 326, 347, 443, 574
DdeI CTNAG 2 cut(s) 271, 545
DpnI GATC 2 cut(s) 135, 287
DpnII GATC 2 cut(s) 133, 285
EaeI YGGCCR 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 381
Eco72I CACGTG 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 381
FaiI YATR 7 cut(s) 240, 318, 588, 640, 659, 734, 743
Fnu4HI GCNGC 2 cut(s) 519, 528
FokI GGATG 2 cut(s) 43, 739
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 2 cut(s) 519, 528
GlaI GCGC 1 cut(s) 552
GluI GCNGC 2 cut(s) 519, 528
GsuI CTGGAG 3 cut(s) 188, 507, 633
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 204, 291
HhaI GCGC 1 cut(s) 553
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 3 cut(s) 107, 310, 502
HpaII CCGG 2 cut(s) 204, 291
HphI GGTGA 5 cut(s) 172, 184, 193, 280, 317
Hpy166II GTNNAC 2 cut(s) 54, 220
Hpy188I TCNGA 3 cut(s) 274, 507, 636
Hpy188III TCNNGA 3 cut(s) 137, 473, 650
Hpy8I GTNNAC 2 cut(s) 54, 220
HpyAV CCTTC 4 cut(s) 134, 151, 448, 502
HpyCH4III ACNGT 2 cut(s) 199, 325
HpyCH4IV ACGT 2 cut(s) 70, 234
HpyCH4V TGCA 3 cut(s) 34, 605, 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 524, 527, 746
HpyF3I CTNAG 2 cut(s) 271, 545
HpySE526I ACGT 2 cut(s) 70, 234
HspAI GCGC 1 cut(s) 551
Kzo9I GATC 2 cut(s) 133, 285
LmnI GCTCC 5 cut(s) 376, 403, 430, 457, 526
Lsp1109I GCAGC 2 cut(s) 505, 539
LweI GCATC 3 cut(s) 21, 592, 619
MaeII ACGT 2 cut(s) 70, 234
MaeIII GTNAC 2 cut(s) 199, 319
MalI GATC 2 cut(s) 135, 287
MboI GATC 2 cut(s) 133, 285
MboII GAAGA 4 cut(s) 30, 88, 116, 686
MhlI GDGCHC 1 cut(s) 381
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 1 cut(s) 250
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 7 cut(s) 8, 97, 163, 219, 238, 664, 697
Mox20I TGGCCA 1 cut(s) 117
MroXI GAANNNNTTC 2 cut(s) 252, 468
MscI TGGCCA 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 2 cut(s) 204, 291
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 749
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 524, 527, 746
NdeII GATC 2 cut(s) 133, 285
NlaIV GGNNCC 5 cut(s) 354, 378, 405, 432, 459
NmuCI GTSAC 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 71
PctI GAATGC 1 cut(s) 749
PdmI GAANNNNTTC 2 cut(s) 252, 468
PfeI GAWTC 2 cut(s) 310, 502
PkrI GCNGC 2 cut(s) 520, 529
PleI GAGTC 1 cut(s) 101
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 85
PspCI CACGTG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 5 cut(s) 354, 378, 405, 432, 459
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 4 cut(s) 327, 348, 444, 575
RsaNI GTAC 4 cut(s) 326, 347, 443, 574
RseI CAYNNNNRTG 1 cut(s) 71
SatI GCNGC 2 cut(s) 519, 528
Sau3AI GATC 2 cut(s) 133, 285
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 3 cut(s) 21, 592, 619
SfcI CTRYAG 1 cut(s) 741
SmiMI CAYNNNNRTG 1 cut(s) 71
Sse9I AATT 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 199, 325
TaiI ACGT 2 cut(s) 73, 237
TaqI TCGA 3 cut(s) 132, 279, 513
TasI AATT 1 cut(s) 250
TatI WGTACW 3 cut(s) 346, 442, 573
TfiI GAWTC 2 cut(s) 310, 502
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 518, 527
Tsp45I GTSAC 1 cut(s) 199
XapI RAATTY 1 cut(s) 250
XmnI GAANNNNTTC 2 cut(s) 252, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.