RLG00000005247

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013677
Physical Location & Seq
Forward (+)
66208370 .. 66209260
891 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005247

Sequence Viewer

Length: 804 bp
ATGGCAAATGTTAGAGGAAGAACATCAGGGATGCACAGGGGAGAGAGATTGTCAACATTCACGCCCACACGTGTGAAGATTGTTCCTGGTTCTGGCTGGACAGAGGACTCTAATGGCCATTATCTTCTTGTCGATCTGGAAGGTTTCAACAAGGAGAAGGTGAAGCTGGAGGTGAATACCACAGATGGACATCTAACAGTCACCGGAGAAAGGCAAGTGAACGAGAGGGAACGTGTCTATATTGAGGAAAATTTTCCAATACCAGCAAACTCAGATGTTGATAAGATCACCGGAAGGTTTGATGGTGAGATTCTCTATGTAACTGTACCAAAGGTTGTAGCAGTAGTACAAGAAAACAAGGAACCTGAAATAGTAGAAGCAAAGGAGCCCGAAATAATAGAAGCAAAAAAGGAGCCTGAAATGGTAGAAGCAAAGATGGAGCCTGAAATAGTACAACCAAAGGAGCCTGAAATAGTTCAAGAAAAGAACAGACCTGAAATAATAGAGAATCAGAAGGTCGAAGCTGCTCCAGCAGCAGAAAACACACACCTAAGAGCGCCCAAAAACAACGATGGAAAGTACAGAAACAAATATGCTGGCACAAATGATGCACAGCCAAACAGATTATTAGGCAATGCTTGGACATACTTTTCTCCAGAGAATATACGGAAGTGGGAGGAAGATAAAGGCATTTTGAGAAGTGCAATGGAGGTGTTGAGCAAAAACAAAGAGATGGTTATAACAGCTATAGTAGCATTCTCGTTAGGGATGTTAGTATCTCAAAAGTTCAAAACAGGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

268

Amino Acids

30.19

Weight (kDa)

5.43

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 119 2.4e-08 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 740
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 250
AcvI CACGTG 1 cut(s) 71
AfaI GTAC 4 cut(s) 327, 348, 453, 581
AfiI CCNNNNNNNGG 2 cut(s) 92, 210
AflIII ACRYGT 3 cut(s) 68, 70, 232
AgsI TTSAA 3 cut(s) 148, 479, 790
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 3 cut(s) 166, 524, 746
AluI AGCT 3 cut(s) 166, 524, 746
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 524, 533
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 2 cut(s) 252, 474
AspLEI GCGC 1 cut(s) 559
AsuHPI GGTGA 5 cut(s) 172, 184, 193, 280, 317
BalI TGGCCA 1 cut(s) 117
BanII GRGCYC 1 cut(s) 390
BbrPI CACGTG 1 cut(s) 71
BbvI GCAGC 2 cut(s) 511, 545
BccI CCATC 5 cut(s) 179, 296, 430, 566, 727
BciT130I CCWGG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 560
BisI GCNGC 2 cut(s) 525, 534
BlsI GCNGC 2 cut(s) 526, 535
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 5 cut(s) 363, 387, 414, 441, 465
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 21, 598
BpmI CTGGAG 3 cut(s) 188, 513, 639
BsaAI YACGTR 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 189
BsaWI WCCGGW 2 cut(s) 203, 290
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 210
Bse3DI GCAATG 2 cut(s) 640, 711
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 36, 774
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 92, 210
BseMI GCAATG 2 cut(s) 640, 711
BseMII CTCAG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 511, 545
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 204, 291
BslI CCNNNNNNNGG 2 cut(s) 92, 210
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 2 cut(s) 133, 285
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 284
BspLI GGNNCC 5 cut(s) 363, 387, 414, 441, 465
BsrDI GCAATG 2 cut(s) 640, 711
BssMI GATC 2 cut(s) 133, 285
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 199, 325
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 598
BstDEI CTNAG 2 cut(s) 271, 551
BstF5I GGATG 2 cut(s) 36, 774
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 2 cut(s) 136, 288
BstMBI GATC 2 cut(s) 133, 285
BstMWI GCNNNNNNNGC 3 cut(s) 530, 533, 752
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 747
BstV1I GCAGC 2 cut(s) 511, 545
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 2 cut(s) 36, 774
Cac8I GCNNGC 1 cut(s) 598
CfoI GCGC 1 cut(s) 559
Csp6I GTAC 4 cut(s) 326, 347, 452, 580
CviQI GTAC 4 cut(s) 326, 347, 452, 580
DdeI CTNAG 2 cut(s) 271, 551
DpnI GATC 2 cut(s) 135, 287
DpnII GATC 2 cut(s) 133, 285
EaeI YGGCCR 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 390
Eco72I CACGTG 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 390
FaiI YATR 7 cut(s) 240, 318, 594, 646, 665, 740, 749
Fnu4HI GCNGC 2 cut(s) 525, 534
FokI GGATG 2 cut(s) 43, 781
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 2 cut(s) 525, 534
GlaI GCGC 1 cut(s) 558
GluI GCNGC 2 cut(s) 525, 534
GsuI CTGGAG 3 cut(s) 188, 513, 639
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 204, 291
HhaI GCGC 1 cut(s) 559
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 3 cut(s) 107, 310, 508
HpaII CCGG 2 cut(s) 204, 291
HphI GGTGA 5 cut(s) 172, 184, 193, 280, 317
Hpy166II GTNNAC 2 cut(s) 54, 220
Hpy188I TCNGA 2 cut(s) 274, 513
Hpy188III TCNNGA 3 cut(s) 137, 479, 656
Hpy8I GTNNAC 2 cut(s) 54, 220
HpyAV CCTTC 4 cut(s) 134, 151, 288, 508
HpyCH4III ACNGT 2 cut(s) 199, 325
HpyCH4IV ACGT 2 cut(s) 70, 232
HpyCH4V TGCA 3 cut(s) 34, 611, 704
HpyF10VI GCNNNNNNNGC 3 cut(s) 530, 533, 752
HpyF3I CTNAG 2 cut(s) 271, 551
HpySE526I ACGT 2 cut(s) 70, 232
HspAI GCGC 1 cut(s) 557
Kzo9I GATC 2 cut(s) 133, 285
LmnI GCTCC 5 cut(s) 385, 412, 439, 463, 532
Lsp1109I GCAGC 2 cut(s) 511, 545
LweI GCATC 2 cut(s) 21, 598
MaeII ACGT 2 cut(s) 70, 232
MaeIII GTNAC 2 cut(s) 199, 319
MalI GATC 2 cut(s) 135, 287
MboI GATC 2 cut(s) 133, 285
MboII GAAGA 4 cut(s) 30, 88, 116, 692
MhlI GDGCHC 1 cut(s) 390
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 1 cut(s) 250
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 7 cut(s) 8, 97, 163, 219, 238, 670, 703
Mox20I TGGCCA 1 cut(s) 117
MroXI GAANNNNTTC 2 cut(s) 252, 474
MscI TGGCCA 1 cut(s) 117
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 117
MspI CCGG 2 cut(s) 204, 291
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 530, 533, 752
NdeII GATC 2 cut(s) 133, 285
NlaIV GGNNCC 5 cut(s) 363, 387, 414, 441, 465
NmuCI GTSAC 1 cut(s) 199
OliI CACNNNNGTG 1 cut(s) 71
PctI GAATGC 1 cut(s) 755
PdmI GAANNNNTTC 2 cut(s) 252, 474
PfeI GAWTC 2 cut(s) 310, 508
PkrI GCNGC 2 cut(s) 526, 535
PleI GAGTC 1 cut(s) 101
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 71
PsiI TTATAA 1 cut(s) 740
Psp6I CCWGG 1 cut(s) 85
PspCI CACGTG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 5 cut(s) 363, 387, 414, 441, 465
RsaI GTAC 4 cut(s) 327, 348, 453, 581
RsaNI GTAC 4 cut(s) 326, 347, 452, 580
RseI CAYNNNNRTG 1 cut(s) 71
SatI GCNGC 2 cut(s) 525, 534
Sau3AI GATC 2 cut(s) 133, 285
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 2 cut(s) 21, 598
SfcI CTRYAG 1 cut(s) 747
SmiMI CAYNNNNRTG 1 cut(s) 71
Sse9I AATT 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 199, 325
TaiI ACGT 2 cut(s) 73, 235
TaqI TCGA 2 cut(s) 132, 519
TasI AATT 1 cut(s) 250
TatI WGTACW 3 cut(s) 346, 451, 579
TfiI GAWTC 2 cut(s) 310, 508
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 524, 533
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 1 cut(s) 682
XapI RAATTY 1 cut(s) 250
XmnI GAANNNNTTC 2 cut(s) 252, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.