MD06G1188500.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013677
Physical Location & Seq
Reverse (-)
32557693 .. 32558803
1111 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1188500.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGCAGATATCAGAGGATCAATGAGAGCTATGGGAGAGAGATTACTGCCATCGACACCCACACCCATGCTCGAAAAGATTGTTCCTTCCTCAGACTGGACAGAAGACTGCAATGGCCATTACCTTCTCGTTGATCTTCCAGATTTTAAAAAGGAGGAGGTCAGGCTTGAGGTGAATATCTCAGCTGGCCACATAACAATCAGCGGAGAAAGGCAAGTGAACGAAAAGAAAACCGAGTATTTTAAGCAGAAGTTTACCCTTCCACCAAACTCAGATGTTGATAAGATCACCGGAAAATTTGACGGTGAGATTCTCTATGTCACCGTACCAAAGGTGGCAACAGCGGTCGAAGAAAAAAGGAAAGAACCTGAAATAAAGTATGAAAATGTCCAAGGCAGCGCTGCTGCTGTCACGGAACCTCCTCAAATAGGGAAGAATGAAAATGTGGAAGGCTTAAGACGCAAAAATGAAGGAGAGCAAGGCTCAAACAAAGTTGATGGCATAAAGTATGGAGAGATAAGAAAATTATTAGGAGGAGGAAATGGGCACATATTCTTTTCTGCAGAAGATATAAGGAAATGGGAGGAGGAAGGAGGGAATATTATAAAGACTGCAATGGAGATGTTGAACAAAAACAAAGGGATAGTTTTGACTGCTGTGGTTGCATTTTCATTAGGGATGCTAGTTTCTCGTAAGTTCCAATCAGCTGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

239

Amino Acids

26.43

Weight (kDa)

5.83

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 122 2.2e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AciI CCGC 2 cut(s) 204, 344
AclWI GGATC 1 cut(s) 25
AcoI YGGCCR 2 cut(s) 115, 187
AcsI RAATTY 1 cut(s) 296
AfaI GTAC 1 cut(s) 327
AfeI AGCGCT 1 cut(s) 400
AfiI CCNNNNNNNGG 2 cut(s) 96, 428
AflII CTTAAG 1 cut(s) 454
AgsI TTSAA 1 cut(s) 628
AluBI AGCT 3 cut(s) 29, 185, 707
AluI AGCT 3 cut(s) 29, 185, 707
AlwI GGATC 1 cut(s) 25
Aor51HI AGCGCT 1 cut(s) 400
AoxI GGCC 2 cut(s) 115, 187
ApeKI GCWGC 3 cut(s) 396, 401, 404
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 1 cut(s) 401
AsuHPI GGTGA 4 cut(s) 184, 280, 313, 317
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 2 cut(s) 117, 189
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 388, 391, 408
BccI CCATC 2 cut(s) 58, 491
BfaI CTAG 1 cut(s) 683
BfmI CTRYAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 402
BfrI CTTAAG 1 cut(s) 454
BisI GCNGC 3 cut(s) 397, 402, 405
BlsI GCNGC 3 cut(s) 398, 403, 406
BmiI GGNNCC 1 cut(s) 417
BmsI GCATC 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpuEI CTTGAG 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 391
BsaWI WCCGGW 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 428
Bse1I ACTGG 1 cut(s) 101
Bse3DI GCAATG 2 cut(s) 118, 621
BseDI CCNNGG 1 cut(s) 391
BseGI GGATG 1 cut(s) 684
BseLI CCNNNNNNNGG 2 cut(s) 96, 428
BseMI GCAATG 2 cut(s) 118, 621
BseMII CTCAG 3 cut(s) 105, 195, 285
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 4 cut(s) 170, 411, 549, 599
BseSI GKGCMC 1 cut(s) 549
BseXI GCAGC 3 cut(s) 388, 391, 408
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 117, 189
BsiEI CGRYCG 1 cut(s) 348
BsiSI CCGG 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 96, 428
BsnI GGCC 2 cut(s) 117, 189
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 3 cut(s) 17, 133, 285
BspACI CCGC 2 cut(s) 204, 344
BspANI GGCC 2 cut(s) 117, 189
BspCNI CTCAG 3 cut(s) 104, 194, 284
BspLI GGNNCC 1 cut(s) 417
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 1 cut(s) 25
BspTI CTTAAG 1 cut(s) 454
BsrDI GCAATG 2 cut(s) 118, 621
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 391
BssMI GATC 3 cut(s) 17, 133, 285
BssT1I CCWWGG 1 cut(s) 391
Bst4CI ACNGT 2 cut(s) 305, 325
BstAFI CTTAAG 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 3 cut(s) 91, 181, 271
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 1 cut(s) 684
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 3 cut(s) 20, 136, 288
BstMBI GATC 3 cut(s) 17, 133, 285
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 2 cut(s) 459, 662
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 549
BstV1I GCAGC 3 cut(s) 388, 391, 408
BstV2I GAAGAC 1 cut(s) 111
BstXI CCANNNNNNTGG 1 cut(s) 707
BsuRI GGCC 2 cut(s) 117, 189
BtsCI GGATG 1 cut(s) 684
Cac8I GCNNGC 1 cut(s) 187
CfoI GCGC 1 cut(s) 401
CseI GACGC 1 cut(s) 468
Csp6I GTAC 1 cut(s) 326
CviAII CATG 1 cut(s) 67
CviJI RGCY 8 cut(s) 29, 117, 166, 185, 189, 453, 483, 707
CviKI_1 RGCY 8 cut(s) 29, 117, 166, 185, 189, 453, 483, 707
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 3 cut(s) 91, 181, 271
DpnI GATC 3 cut(s) 19, 135, 287
DpnII GATC 3 cut(s) 17, 133, 285
DraI TTTAAA 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 115, 187
Eco130I CCWWGG 1 cut(s) 391
Eco32I GATATC 1 cut(s) 10
Eco47III AGCGCT 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 1 cut(s) 70
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 397, 402, 405
FokI GGATG 1 cut(s) 691
Fsp4HI GCNGC 3 cut(s) 397, 402, 405
FspBI CTAG 1 cut(s) 683
GlaI GCGC 1 cut(s) 400
GluI GCNGC 3 cut(s) 397, 402, 405
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 2 cut(s) 117, 189
HapII CCGG 1 cut(s) 291
HgaI GACGC 1 cut(s) 468
HhaI GCGC 1 cut(s) 401
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HinfI GANTC 1 cut(s) 310
HpaII CCGG 1 cut(s) 291
HphI GGTGA 4 cut(s) 184, 280, 313, 317
Hpy166II GTNNAC 2 cut(s) 220, 255
Hpy188I TCNGA 3 cut(s) 14, 94, 274
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 2 cut(s) 220, 255
HpyAV CCTTC 6 cut(s) 96, 134, 269, 443, 464, 584
HpyCH4III ACNGT 2 cut(s) 305, 325
HpyCH4V TGCA 4 cut(s) 111, 563, 614, 665
HpyF10VI GCNNNNNNNGC 2 cut(s) 459, 662
HpyF3I CTNAG 3 cut(s) 91, 181, 271
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 399
Kzo9I GATC 3 cut(s) 17, 133, 285
LpnPI CCDG 7 cut(s) 82, 148, 153, 171, 304, 381, 693
Lsp1109I GCAGC 3 cut(s) 388, 391, 408
LweI GCATC 1 cut(s) 669
MaeI CTAG 1 cut(s) 683
MaeIII GTNAC 2 cut(s) 319, 409
MalI GATC 3 cut(s) 19, 135, 287
MboI GATC 3 cut(s) 17, 133, 285
MboII GAAGA 5 cut(s) 116, 128, 362, 445, 578
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 2 cut(s) 117, 189
MluCI AATT 2 cut(s) 296, 524
MluNI TGGCCA 2 cut(s) 117, 189
Mox20I TGGCCA 2 cut(s) 117, 189
MscI TGGCCA 2 cut(s) 117, 189
MseI TTAA 3 cut(s) 147, 243, 455
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 2 cut(s) 117, 189
MspA1I CMGCKG 4 cut(s) 185, 204, 344, 707
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 459, 662
NdeII GATC 3 cut(s) 17, 133, 285
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 417
NmuCI GTSAC 2 cut(s) 319, 409
PfeI GAWTC 1 cut(s) 310
PkrI GCNGC 3 cut(s) 398, 403, 406
PsiI TTATAA 1 cut(s) 605
PspN4I GGNNCC 1 cut(s) 417
PstI CTGCAG 1 cut(s) 565
PvuII CAGCTG 2 cut(s) 185, 707
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 3 cut(s) 147, 243, 455
SatI GCNGC 3 cut(s) 397, 402, 405
Sau3AI GATC 3 cut(s) 17, 133, 285
SduI GDGCHC 1 cut(s) 549
SetI ASST 9 cut(s) 31, 126, 162, 174, 187, 336, 370, 421, 709
SfaNI GCATC 1 cut(s) 669
SfcI CTRYAG 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 2 cut(s) 167, 454
SmoI CTYRAG 2 cut(s) 167, 454
Sse9I AATT 2 cut(s) 296, 524
SsiI CCGC 2 cut(s) 204, 344
SspI AATATT 1 cut(s) 601
SspMI CTAG 1 cut(s) 683
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 2 cut(s) 305, 325
TaqI TCGA 3 cut(s) 53, 72, 348
TasI AATT 2 cut(s) 296, 524
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 3 cut(s) 147, 243, 455
Tru9I TTAA 3 cut(s) 147, 243, 455
TseFI GTSAC 2 cut(s) 319, 409
TseI GCWGC 3 cut(s) 396, 401, 404
Tsp45I GTSAC 2 cut(s) 319, 409
TspDTI ATGAA 4 cut(s) 396, 453, 483, 660
TspGWI ACGGA 1 cut(s) 428
Vha464I CTTAAG 1 cut(s) 454
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 1 cut(s) 296
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.