Rmu_sc0029934.1_g000001

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0029934.1
OG0013677
Physical Location & Seq
Reverse (-)
617 .. 1480
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0029934.1_g000001.1.cds

Sequence Viewer

Length: 777 bp
atgcacaggggagagagattgccaacattcacgcccacacgtgtgaagattgttcctggttctggctggacagaggactctaatggccattatcttcttgtcgatctggaaggtttcaacaaggagaaggtgaagctggaagtgaataccacagagggacatctaacagtcaccggagaaaggcaagtgaacgtgagggaacatgtctatattgaggaaaattttccaataccagcaaactcagatgtcgataagatcaccggaaagtttgatggtgagattctctatgtaactgtaccaaaggttgtagcagtagtacaagaaaacaaggaacctgaaatagtagaagcaaaggagcccgaaataattgtagcaaaaaaggagcctgaaatggtagaagcaaagatggagcctgaaatagtacaaccaaagaaggagcctgaaatagttcaagaaaaacaaagacctgaaataatagagaatcagaaggtcgaagctgttccggcagcagaaaacacacacctaagagcgcccaaaaacaatgatggaaagtacagagacaaatatgatggcacaaatgacgcacagccaaacagattattaggcaatgctcggatatacttttctccggagaatatacggaagtgggaggaagataaaggcattttgagaagtgcaatggaggtgttgagcaaaaacaaagggatggttataacagctatagtagcattctcgttagggatgttagtatctcaaaagttcaaaacaggggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

258

Amino Acids

29.2

Weight (kDa)

5.5

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 713
AccIII TCCGGA 1 cut(s) 628
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 1 cut(s) 220
AcvI CACGTG 1 cut(s) 41
AfaI GTAC 4 cut(s) 297, 318, 423, 554
AfiI CCNNNNNNNGG 2 cut(s) 62, 180
AflIII ACRYGT 3 cut(s) 38, 40, 202
AgsI TTSAA 3 cut(s) 118, 452, 763
AjnI CCWGG 1 cut(s) 55
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 3 cut(s) 136, 497, 719
AluI AGCT 3 cut(s) 136, 497, 719
Alw26I GTCTC 1 cut(s) 552
Aor13HI TCCGGA 1 cut(s) 628
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 506
ApoI RAATTY 1 cut(s) 220
Asp700I GAANNNNTTC 3 cut(s) 222, 447, 498
AspLEI GCGC 1 cut(s) 532
AsuHPI GGTGA 4 cut(s) 142, 163, 250, 287
BalI TGGCCA 1 cut(s) 87
BanII GRGCYC 1 cut(s) 360
BbrPI CACGTG 1 cut(s) 41
BbvI GCAGC 1 cut(s) 518
BccI CCATC 5 cut(s) 266, 400, 539, 563, 700
BciT130I CCWGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 552
BfmI CTRYAG 1 cut(s) 720
BfoI RGCGCY 1 cut(s) 533
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
Bme1390I CCNGG 1 cut(s) 57
BmiI GGNNCC 5 cut(s) 333, 357, 384, 411, 438
BmrFI CCNGG 1 cut(s) 57
BsaAI YACGTR 1 cut(s) 41
BsaWI WCCGGW 3 cut(s) 173, 260, 628
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 180
Bse3DI GCAATG 2 cut(s) 613, 684
BseAI TCCGGA 1 cut(s) 628
BseBI CCWGG 1 cut(s) 57
BseGI GGATG 2 cut(s) 711, 747
BseLI CCNNNNNNNGG 2 cut(s) 62, 180
BseMI GCAATG 2 cut(s) 613, 684
BseMII CTCAG 1 cut(s) 255
BseXI GCAGC 1 cut(s) 518
BshFI GGCC 1 cut(s) 87
BsiSI CCGG 4 cut(s) 174, 261, 503, 629
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 2 cut(s) 62, 180
BsmAI GTCTC 1 cut(s) 552
BsmFI GGGAC 1 cut(s) 171
BsmI GAATGC 1 cut(s) 728
BsnI GGCC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 360
Bsp13I TCCGGA 1 cut(s) 628
Bsp143I GATC 2 cut(s) 103, 255
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 1 cut(s) 254
BspEI TCCGGA 1 cut(s) 628
BspLI GGNNCC 5 cut(s) 333, 357, 384, 411, 438
BsrDI GCAATG 2 cut(s) 613, 684
BssMI GATC 2 cut(s) 103, 255
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 2 cut(s) 169, 295
BstBAI YACGTR 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 241, 524
BstF5I GGATG 2 cut(s) 711, 747
BstH2I RGCGCY 1 cut(s) 533
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 2 cut(s) 106, 258
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 2 cut(s) 103, 255
BstMWI GCNNNNNNNGC 2 cut(s) 503, 725
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 720
BstV1I GCAGC 1 cut(s) 518
BsuRI GGCC 1 cut(s) 87
BtsCI GGATG 2 cut(s) 711, 747
CfoI GCGC 1 cut(s) 532
CseI GACGC 1 cut(s) 590
Csp6I GTAC 4 cut(s) 296, 317, 422, 553
CviAII CATG 1 cut(s) 203
CviQI GTAC 4 cut(s) 296, 317, 422, 553
DdeI CTNAG 2 cut(s) 241, 524
DpnI GATC 2 cut(s) 105, 257
DpnII GATC 2 cut(s) 103, 255
EaeI YGGCCR 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 360
Eco72I CACGTG 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 55
EcoT38I GRGCYC 1 cut(s) 360
FaeI CATG 1 cut(s) 206
FaiI YATR 8 cut(s) 204, 210, 288, 567, 619, 638, 713, 722
FaqI GGGAC 1 cut(s) 171
FatI CATG 1 cut(s) 202
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 2 cut(s) 718, 754
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 1 cut(s) 507
GlaI GCGC 1 cut(s) 531
GluI GCNGC 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 533
HaeIII GGCC 1 cut(s) 87
HapII CCGG 4 cut(s) 174, 261, 503, 629
HgaI GACGC 1 cut(s) 590
HhaI GCGC 1 cut(s) 532
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HinfI GANTC 3 cut(s) 77, 280, 481
HpaII CCGG 4 cut(s) 174, 261, 503, 629
HphI GGTGA 4 cut(s) 142, 163, 250, 287
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 3 cut(s) 244, 486, 615
Hpy188III TCNNGA 3 cut(s) 107, 452, 629
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 4 cut(s) 104, 121, 427, 481
HpyCH4III ACNGT 2 cut(s) 169, 295
HpyCH4IV ACGT 2 cut(s) 40, 192
HpyCH4V TGCA 2 cut(s) 4, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 503, 725
HpyF3I CTNAG 2 cut(s) 241, 524
HpySE526I ACGT 2 cut(s) 40, 192
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 1 cut(s) 530
Kpn2I TCCGGA 1 cut(s) 628
Kzo9I GATC 2 cut(s) 103, 255
LmnI GCTCC 4 cut(s) 355, 382, 409, 436
Lsp1109I GCAGC 1 cut(s) 518
MaeII ACGT 2 cut(s) 40, 192
MaeIII GTNAC 2 cut(s) 169, 289
MalI GATC 2 cut(s) 105, 257
MboI GATC 2 cut(s) 103, 255
MboII GAAGA 3 cut(s) 58, 86, 665
MhlI GDGCHC 1 cut(s) 360
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 2 cut(s) 220, 366
MluNI TGGCCA 1 cut(s) 87
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 6 cut(s) 67, 148, 189, 208, 643, 676
Mox20I TGGCCA 1 cut(s) 87
MroI TCCGGA 1 cut(s) 628
MroXI GAANNNNTTC 3 cut(s) 222, 447, 498
MscI TGGCCA 1 cut(s) 87
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 87
MspI CCGG 4 cut(s) 174, 261, 503, 629
MspR9I CCNGG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 728
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 2 cut(s) 503, 725
NdeII GATC 2 cut(s) 103, 255
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 5 cut(s) 333, 357, 384, 411, 438
NmuCI GTSAC 1 cut(s) 169
NspI RCATGY 1 cut(s) 206
OliI CACNNNNGTG 1 cut(s) 41
PciI ACATGT 1 cut(s) 202
PctI GAATGC 1 cut(s) 728
PdmI GAANNNNTTC 3 cut(s) 222, 447, 498
PfeI GAWTC 2 cut(s) 280, 481
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 71
PmaCI CACGTG 1 cut(s) 41
PmlI CACGTG 1 cut(s) 41
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 41
PscI ACATGT 1 cut(s) 202
PsiI TTATAA 1 cut(s) 713
Psp6I CCWGG 1 cut(s) 55
PspCI CACGTG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 5 cut(s) 333, 357, 384, 411, 438
RsaI GTAC 4 cut(s) 297, 318, 423, 554
RsaNI GTAC 4 cut(s) 296, 317, 422, 553
RseI CAYNNNNRTG 1 cut(s) 41
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 2 cut(s) 103, 255
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 1 cut(s) 360
SfcI CTRYAG 1 cut(s) 720
SmiMI CAYNNNNRTG 1 cut(s) 41
Sse9I AATT 2 cut(s) 220, 366
StyD4I CCNGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 169, 295
TaiI ACGT 2 cut(s) 43, 195
TaqI TCGA 3 cut(s) 102, 249, 492
TasI AATT 2 cut(s) 220, 366
TatI WGTACW 3 cut(s) 316, 421, 552
TfiI GAWTC 2 cut(s) 280, 481
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 1 cut(s) 169
TspGWI ACGGA 1 cut(s) 655
XapI RAATTY 1 cut(s) 220
XceI RCATGY 1 cut(s) 206
XmnI GAANNNNTTC 3 cut(s) 222, 447, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.