MD14G1194900.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013677
Physical Location & Seq
Reverse (-)
28585341 .. 28586645
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1194900.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGCAAATATCAGAGGATCAATGAGCGCTTTGGAAGAGAGATTACGGCCATCGACACCCACACGCATGCGCGAAAAGATTGTTCCTTCATCAGACTGGACGGAGGACTCCAATTGTCATTACCTTCTCGTTGATCTTCCAGATTTTAAAAAGGAGGAGGTGAGGGTTGAGGTGAATATCTCAGCCGGCCATATAACAATCGGAGGAGAAAGGCAAGTGAACGAAAGGATATCCCAGTATTTTGAGCAGATTTTCACCCTTCCGCAACACTCAGATGTCAATAAGATCATCGGAAAATTTGACGGTGAGATTCTATATGTCACCGTACCAAAGGTGGCAGCAGCGGTTGAAGAACAGAAGAAAGAACCTGAAATAGAGAATGAAAATGTCCTAGGCAGCACCGCTGCTGCCACGGAACCTCCTCAAGTAGAGAAGAATGAAACTGTGGAAGGCTTAAGAGTTAAGACCCAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

159

Amino Acids

17.85

Weight (kDa)

5.1

Isoelectric Point (pI)

60.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 32 - 122 1.1e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 72
AciI CCGC 3 cut(s) 263, 344, 402
AclWI GGATC 1 cut(s) 25
AcoI YGGCCR 2 cut(s) 47, 187
AcsI RAATTY 1 cut(s) 296
AfaI GTAC 1 cut(s) 327
AfeI AGCGCT 1 cut(s) 28
AflII CTTAAG 1 cut(s) 454
AgsI TTSAA 1 cut(s) 350
AlwI GGATC 1 cut(s) 25
Aor51HI AGCGCT 1 cut(s) 28
AoxI GGCC 2 cut(s) 47, 187
ApeKI GCWGC 5 cut(s) 338, 341, 396, 404, 407
ApoI RAATTY 1 cut(s) 296
AspA2I CCTAGG 1 cut(s) 391
AspLEI GCGC 2 cut(s) 29, 72
AsuHPI GGTGA 5 cut(s) 172, 184, 247, 313, 317
AvrII CCTAGG 1 cut(s) 391
BarI GAAGNNNNNNTAC 2 cut(s) 27, 59
BbvI GCAGC 5 cut(s) 350, 353, 391, 394, 408
BccI CCATC 1 cut(s) 58
BceAI ACGGC 1 cut(s) 62
BfaI CTAG 1 cut(s) 392
BfoI RGCGCY 1 cut(s) 30
BfrI CTTAAG 1 cut(s) 454
BisI GCNGC 5 cut(s) 339, 342, 397, 405, 408
BlnI CCTAGG 1 cut(s) 391
BlsI GCNGC 5 cut(s) 340, 343, 398, 406, 409
BmiI GGNNCC 1 cut(s) 417
BmrI ACTGGG 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 391, 411
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 101, 235
BseDI CCNNGG 2 cut(s) 391, 411
BseMII CTCAG 2 cut(s) 195, 285
BseNI ACTGG 2 cut(s) 101, 235
BseRI GAGGAG 3 cut(s) 170, 219, 411
BseXI GCAGC 5 cut(s) 350, 353, 391, 394, 408
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 49, 189
BsiSI CCGG 1 cut(s) 186
BsnI GGCC 2 cut(s) 49, 189
Bsp143I GATC 3 cut(s) 17, 133, 285
BspACI CCGC 3 cut(s) 263, 344, 402
BspANI GGCC 2 cut(s) 49, 189
BspCNI CTCAG 2 cut(s) 194, 284
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 417
BspPI GGATC 1 cut(s) 25
BspTI CTTAAG 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 101, 235
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 391, 411
BssMI GATC 3 cut(s) 17, 133, 285
BssT1I CCWWGG 1 cut(s) 391
Bst4CI ACNGT 3 cut(s) 305, 325, 445
Bst6I CTCTTC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 68, 187
BstDEI CTNAG 2 cut(s) 181, 271
BstDSI CCRYGG 1 cut(s) 411
BstFNI CGCG 1 cut(s) 72
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 2 cut(s) 29, 72
BstKTI GATC 3 cut(s) 20, 136, 288
BstMBI GATC 3 cut(s) 17, 133, 285
BstNSI RCATGY 1 cut(s) 70
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 5 cut(s) 350, 353, 391, 394, 408
BsuRI GGCC 2 cut(s) 49, 189
BtgI CCRYGG 1 cut(s) 411
Cac8I GCNNGC 2 cut(s) 68, 187
CfoI GCGC 2 cut(s) 29, 72
Cfr10I RCCGGY 1 cut(s) 185
Csp6I GTAC 1 cut(s) 326
CviAII CATG 1 cut(s) 67
CviJI RGCY 4 cut(s) 49, 185, 189, 453
CviKI_1 RGCY 4 cut(s) 49, 185, 189, 453
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 2 cut(s) 181, 271
DpnI GATC 3 cut(s) 19, 135, 287
DpnII GATC 3 cut(s) 17, 133, 285
DraI TTTAAA 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 47, 187
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 391
Eco32I GATATC 1 cut(s) 231
Eco47III AGCGCT 1 cut(s) 28
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 1 cut(s) 70
FaiI YATR 5 cut(s) 68, 192, 194, 316, 318
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 339, 342, 397, 405, 408
Fsp4HI GCNGC 5 cut(s) 339, 342, 397, 405, 408
FspBI CTAG 1 cut(s) 392
GlaI GCGC 2 cut(s) 28, 71
GluI GCNGC 5 cut(s) 339, 342, 397, 405, 408
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 2 cut(s) 49, 189
HapII CCGG 1 cut(s) 186
HhaI GCGC 2 cut(s) 29, 72
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 2 cut(s) 27, 70
HinP1I GCGC 2 cut(s) 27, 70
HinfI GANTC 2 cut(s) 107, 310
HpaII CCGG 1 cut(s) 186
HphI GGTGA 5 cut(s) 172, 184, 247, 313, 317
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 5 cut(s) 14, 94, 203, 274, 293
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 4 cut(s) 96, 134, 269, 443
HpyCH4III ACNGT 3 cut(s) 305, 325, 445
HpyF3I CTNAG 2 cut(s) 181, 271
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 2 cut(s) 27, 70
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Kzo9I GATC 3 cut(s) 17, 133, 285
LpnPI CCDG 5 cut(s) 82, 153, 199, 248, 381
Lsp1109I GCAGC 5 cut(s) 350, 353, 391, 394, 408
MaeI CTAG 1 cut(s) 392
MaeIII GTNAC 1 cut(s) 319
MalI GATC 3 cut(s) 19, 135, 287
MboI GATC 3 cut(s) 17, 133, 285
MboII GAAGA 5 cut(s) 47, 128, 362, 370, 445
MfeI CAATTG 1 cut(s) 112
MluCI AATT 2 cut(s) 112, 296
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 9 cut(s) 8, 97, 148, 151, 156, 163, 197, 429, 432
MroNI GCCGGC 1 cut(s) 185
MseI TTAA 3 cut(s) 147, 455, 462
MslI CAYNNNNRTG 2 cut(s) 65, 273
MspA1I CMGCKG 2 cut(s) 344, 404
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 1 cut(s) 186
MunI CAATTG 1 cut(s) 112
MvnI CGCG 1 cut(s) 72
NaeI GCCGGC 1 cut(s) 187
NdeII GATC 3 cut(s) 17, 133, 285
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 1 cut(s) 70
PaeI GCATGC 1 cut(s) 70
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 310
PkrI GCNGC 5 cut(s) 340, 343, 398, 406, 409
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 417
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
RseI CAYNNNNRTG 2 cut(s) 65, 273
SaqAI TTAA 3 cut(s) 147, 455, 462
SatI GCNGC 5 cut(s) 339, 342, 397, 405, 408
Sau3AI GATC 3 cut(s) 17, 133, 285
SchI GAGTC 1 cut(s) 101
SetI ASST 6 cut(s) 126, 162, 174, 336, 370, 421
SmiMI CAYNNNNRTG 2 cut(s) 65, 273
SmlI CTYRAG 2 cut(s) 423, 454
SmoI CTYRAG 2 cut(s) 423, 454
SphI GCATGC 1 cut(s) 70
Sse9I AATT 2 cut(s) 112, 296
SsiI CCGC 3 cut(s) 263, 344, 402
SspMI CTAG 1 cut(s) 392
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 3 cut(s) 305, 325, 445
TaqI TCGA 1 cut(s) 53
TasI AATT 2 cut(s) 112, 296
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 3 cut(s) 147, 455, 462
Tru9I TTAA 3 cut(s) 147, 455, 462
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 5 cut(s) 338, 341, 396, 404, 407
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 78, 396, 453
TspGWI ACGGA 2 cut(s) 116, 428
Vha464I CTTAAG 1 cut(s) 454
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 70
XmaJI CCTAGG 1 cut(s) 391
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.