RchiOBHm_Chr7g0180641

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0013677
Physical Location & Seq
Reverse (-)
2485915 .. 2486969
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16110

Sequence Viewer

Length: 807 bp
ATGGCAAATGTTAGAGGAAGAACATCAGGGATGCACAGGGGAGAGAGATTGTCAACATTCACGCCCACACGTGTGAAGATTGTTCCTGGTTCTGGCTGGACAGAGGACTCTAATGGCCATTATCTTCTTGTCGATCTGGAAGGTTTCAACAAGGAGAAGGTGAAGCTGGAAGTGAATACCACAGAGGGACATCTAACAGTCACCGGAGAAAGGCAAGTGAACGTGAGGGAACATGTCTATATTGAGGAAAATTTTCCAATACCAGCAAACTCAGATGTCGATAAGATCACCGGAAAGTTTGATGGTGAGATTCTCTATGTAACTGTACCAAAGGTTGTAGCAGTAGTACAAGAAAACAAGGAACCTGAAATAGTAGAAGCAAAGGAGCCCGAAATAATTGTAGCAAAAAAGGAGCCTGAAATGGTAGAAGCAAAGATGGAGCCTGAAATAGTACAACCAAAGAAGGAGCCTGAAATAGTTCAAGAAAAACAAAGACCTGAAATAATAGAGAATCAGAAGGTCGAAGCTGTTCAGGCAGCAGAAAACACACACCTAAGAGCGCCCAAAAACAATGATGGAAAGTACAGAGACAAATATGATGGCACAAATGACGCACAGCCAAACAGATTATTAGGCAATGCTCGGATATACTTTTCTCCGGAGAATATACGGAAGTGGGAGGAAGATAAAGGCATTTTGAGAAGTGCAATGGAGGTGTTGAGCAAAAACAAAGGGATGGTTATAACAGCTATAGTAGCATTCTCGTTAGGGATGTTAATATCTCAAAAGTTCAAAACAGGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

268

Amino Acids

30.26

Weight (kDa)

5.75

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 119 4.9e-08 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
AccIII TCCGGA 1 cut(s) 658
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 250
AcvI CACGTG 1 cut(s) 71
AfaI GTAC 4 cut(s) 327, 348, 453, 584
AfiI CCNNNNNNNGG 2 cut(s) 92, 210
AflIII ACRYGT 3 cut(s) 68, 70, 232
AgsI TTSAA 3 cut(s) 148, 482, 793
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 3 cut(s) 166, 527, 749
AluI AGCT 3 cut(s) 166, 527, 749
Alw26I GTCTC 1 cut(s) 582
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 536
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 3 cut(s) 252, 477, 528
AspLEI GCGC 1 cut(s) 562
AsuHPI GGTGA 4 cut(s) 172, 193, 280, 317
BalI TGGCCA 1 cut(s) 117
BanII GRGCYC 1 cut(s) 390
BbrPI CACGTG 1 cut(s) 71
BbvI GCAGC 1 cut(s) 548
BccI CCATC 5 cut(s) 296, 430, 569, 593, 730
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 582
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 1 cut(s) 563
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 5 cut(s) 363, 387, 414, 441, 468
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 71
BsaWI WCCGGW 3 cut(s) 203, 290, 658
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 210
Bse3DI GCAATG 2 cut(s) 643, 714
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 3 cut(s) 36, 741, 777
BseLI CCNNNNNNNGG 2 cut(s) 92, 210
BseMI GCAATG 2 cut(s) 643, 714
BseMII CTCAG 1 cut(s) 285
BseXI GCAGC 1 cut(s) 548
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 3 cut(s) 204, 291, 659
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 92, 210
BsmAI GTCTC 1 cut(s) 582
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 758
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 390
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 2 cut(s) 133, 285
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 284
BspEI TCCGGA 1 cut(s) 658
BspLI GGNNCC 5 cut(s) 363, 387, 414, 441, 468
BsrDI GCAATG 2 cut(s) 643, 714
BssMI GATC 2 cut(s) 133, 285
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 199, 325
BstBAI YACGTR 1 cut(s) 71
BstDEI CTNAG 2 cut(s) 271, 554
BstF5I GGATG 3 cut(s) 36, 741, 777
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 1 cut(s) 562
BstKTI GATC 2 cut(s) 136, 288
BstMAI GTCTC 1 cut(s) 582
BstMBI GATC 2 cut(s) 133, 285
BstMWI GCNNNNNNNGC 2 cut(s) 533, 755
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 750
BstV1I GCAGC 1 cut(s) 548
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 36, 741, 777
CfoI GCGC 1 cut(s) 562
CseI GACGC 1 cut(s) 620
Csp6I GTAC 4 cut(s) 326, 347, 452, 583
CviAII CATG 1 cut(s) 233
CviQI GTAC 4 cut(s) 326, 347, 452, 583
DdeI CTNAG 2 cut(s) 271, 554
DpnI GATC 2 cut(s) 135, 287
DpnII GATC 2 cut(s) 133, 285
EaeI YGGCCR 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 390
Eco72I CACGTG 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 390
FaeI CATG 1 cut(s) 236
FaiI YATR 8 cut(s) 234, 240, 318, 597, 649, 668, 743, 752
FaqI GGGAC 1 cut(s) 201
FatI CATG 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 3 cut(s) 43, 748, 784
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 1 cut(s) 537
GlaI GCGC 1 cut(s) 561
GluI GCNGC 1 cut(s) 537
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 1 cut(s) 117
HapII CCGG 3 cut(s) 204, 291, 659
HgaI GACGC 1 cut(s) 620
HhaI GCGC 1 cut(s) 562
Hin1II CATG 1 cut(s) 236
Hin6I GCGC 1 cut(s) 560
HinP1I GCGC 1 cut(s) 560
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 3 cut(s) 107, 310, 511
HpaII CCGG 3 cut(s) 204, 291, 659
HphI GGTGA 4 cut(s) 172, 193, 280, 317
Hpy166II GTNNAC 2 cut(s) 54, 220
Hpy188I TCNGA 3 cut(s) 274, 516, 645
Hpy188III TCNNGA 3 cut(s) 137, 482, 659
Hpy8I GTNNAC 2 cut(s) 54, 220
HpyAV CCTTC 4 cut(s) 134, 151, 457, 511
HpyCH4III ACNGT 2 cut(s) 199, 325
HpyCH4IV ACGT 2 cut(s) 70, 222
HpyCH4V TGCA 2 cut(s) 34, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 533, 755
HpyF3I CTNAG 2 cut(s) 271, 554
HpySE526I ACGT 2 cut(s) 70, 222
Hsp92II CATG 1 cut(s) 236
HspAI GCGC 1 cut(s) 560
Kpn2I TCCGGA 1 cut(s) 658
Kzo9I GATC 2 cut(s) 133, 285
LmnI GCTCC 4 cut(s) 385, 412, 439, 466
Lsp1109I GCAGC 1 cut(s) 548
LweI GCATC 1 cut(s) 21
MaeII ACGT 2 cut(s) 70, 222
MaeIII GTNAC 2 cut(s) 199, 319
MalI GATC 2 cut(s) 135, 287
MboI GATC 2 cut(s) 133, 285
MboII GAAGA 4 cut(s) 30, 88, 116, 695
MhlI GDGCHC 1 cut(s) 390
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 2 cut(s) 250, 396
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 7 cut(s) 8, 97, 178, 219, 238, 673, 706
Mox20I TGGCCA 1 cut(s) 117
MroI TCCGGA 1 cut(s) 658
MroXI GAANNNNTTC 3 cut(s) 252, 477, 528
MscI TGGCCA 1 cut(s) 117
MseI TTAA 1 cut(s) 776
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 117
MspI CCGG 3 cut(s) 204, 291, 659
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 1 cut(s) 758
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 533, 755
NdeII GATC 2 cut(s) 133, 285
NlaIII CATG 1 cut(s) 236
NlaIV GGNNCC 5 cut(s) 363, 387, 414, 441, 468
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 1 cut(s) 236
OliI CACNNNNGTG 1 cut(s) 71
PciI ACATGT 1 cut(s) 232
PctI GAATGC 1 cut(s) 758
PdmI GAANNNNTTC 3 cut(s) 252, 477, 528
PfeI GAWTC 2 cut(s) 310, 511
PkrI GCNGC 1 cut(s) 538
PleI GAGTC 1 cut(s) 101
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 71
PscI ACATGT 1 cut(s) 232
PsiI TTATAA 1 cut(s) 743
Psp6I CCWGG 1 cut(s) 85
PspCI CACGTG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 5 cut(s) 363, 387, 414, 441, 468
RsaI GTAC 4 cut(s) 327, 348, 453, 584
RsaNI GTAC 4 cut(s) 326, 347, 452, 583
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 776
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 2 cut(s) 133, 285
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 1 cut(s) 21
SfcI CTRYAG 1 cut(s) 750
SmiMI CAYNNNNRTG 1 cut(s) 71
Sse9I AATT 2 cut(s) 250, 396
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 199, 325
TaiI ACGT 2 cut(s) 73, 225
TaqI TCGA 3 cut(s) 132, 279, 522
TasI AATT 2 cut(s) 250, 396
TatI WGTACW 3 cut(s) 346, 451, 582
TfiI GAWTC 2 cut(s) 310, 511
Tru1I TTAA 1 cut(s) 776
Tru9I TTAA 1 cut(s) 776
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 1 cut(s) 199
TspGWI ACGGA 1 cut(s) 685
XapI RAATTY 1 cut(s) 250
XceI RCATGY 1 cut(s) 236
XmnI GAANNNNTTC 3 cut(s) 252, 477, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.