Rh7DG036000

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013677
Physical Location & Seq
Reverse (-)
2660788 .. 2664835
4048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG036000.1

Sequence Viewer

Length: 894 bp
ATGGCAAATGTTAGAGGAAGAACATCAGGGATGCACAGGGGAGAGAGATTGTCAACATTCACGCCCACACGTGTGAAGATTGTTCCTGGTTCTGGCTGGACAGAGGACTCTAATGGCCATTATCTTCTTGTCGATCTGGAAGGTATTCATATTGTTTTTAATCCCCATTGCATGCTTGATGATATGGAATCTGCATTCTCACTTTCTTTGGCTCTTAAATGTGTTTTAGGTTTCAACAAGGAGAAGGTGAAGCTGGAAGTGAATACCACAGAGGGACATCTAACAGTCACCGGAGAAAGGCAAGTGAACGTGAGGGAACATGTCTATATTGAGGAAAATTTTCCAATACCAGCAAACTCAGATGTCGATAAGATCACCGGAAAGTTTGATGGTGAGATTCTCTATGTAACTGTACCAAAGGTTGTAGCAGTAGTACAAGAAAACAAGGAACCTGAAATAGTAGAAGCAAAGGAGCCCGAAATAATTGTAGCAAAAAAGGAGCCTGAAATGGTAGAAGCAAAGATGGAGCCTGAAATAGTACAACCAAAGAAGGAGCCTGAAATAGTTCAAGAAAAACAAAGACCTGAAATAATAGAGAATCAGAAGGTCGAAGCTGTTCAGGCAGCAGAAAACACACACCTAAGAGCGCCCAAAAACAATGATGGAAAGTACAGAGACAAATATGATGGCACAAATGACGCACAGCCAAACAGATTATTAGGCAATGCTCGGATATACTTTTCTCCGGAGAATATACGGAAGTGGGAGGAAGATAAAGGCATTTTGAGAAGTGCAATGGAGGTGTTGAGCAAAAACAAAGGGATGGTTATAACAGCTATAGTAGCATTCTCGTTAGGGATGTTAATATCTCAAAAGTTCAAAACAGGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

297

Amino Acids

33.45

Weight (kDa)

5.67

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AccIII TCCGGA 1 cut(s) 745
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 337
AcvI CACGTG 1 cut(s) 71
AfaI GTAC 4 cut(s) 414, 435, 540, 671
AfiI CCNNNNNNNGG 2 cut(s) 92, 297
AflIII ACRYGT 3 cut(s) 68, 70, 319
AgsI TTSAA 3 cut(s) 235, 569, 880
AjnI CCWGG 1 cut(s) 85
AleI CACNNNNGTG 1 cut(s) 71
AluBI AGCT 3 cut(s) 253, 614, 836
AluI AGCT 3 cut(s) 253, 614, 836
Alw26I GTCTC 1 cut(s) 669
Aor13HI TCCGGA 1 cut(s) 745
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 623
ApoI RAATTY 1 cut(s) 337
Asp700I GAANNNNTTC 4 cut(s) 144, 339, 564, 615
AspLEI GCGC 1 cut(s) 649
AsuHPI GGTGA 4 cut(s) 259, 280, 367, 404
BalI TGGCCA 1 cut(s) 117
BanII GRGCYC 1 cut(s) 477
BbrPI CACGTG 1 cut(s) 71
BbvI GCAGC 1 cut(s) 635
BccI CCATC 5 cut(s) 383, 517, 656, 680, 817
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 650
BisI GCNGC 1 cut(s) 624
BlsI GCNGC 1 cut(s) 625
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 5 cut(s) 450, 474, 501, 528, 555
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 71
BsaWI WCCGGW 3 cut(s) 290, 377, 745
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 297
Bse3DI GCAATG 3 cut(s) 166, 730, 801
BseAI TCCGGA 1 cut(s) 745
BseBI CCWGG 1 cut(s) 87
BseGI GGATG 3 cut(s) 36, 828, 864
BseLI CCNNNNNNNGG 2 cut(s) 92, 297
BseMI GCAATG 3 cut(s) 166, 730, 801
BseMII CTCAG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 635
BshFI GGCC 1 cut(s) 117
BsiSI CCGG 3 cut(s) 291, 378, 746
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 92, 297
BsmAI GTCTC 1 cut(s) 669
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 2 cut(s) 194, 845
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 477
Bsp13I TCCGGA 1 cut(s) 745
Bsp143I GATC 2 cut(s) 133, 372
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 371
BspEI TCCGGA 1 cut(s) 745
BspLI GGNNCC 5 cut(s) 450, 474, 501, 528, 555
BsrDI GCAATG 3 cut(s) 166, 730, 801
BssMI GATC 2 cut(s) 133, 372
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 286, 412
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 2 cut(s) 358, 641
BstF5I GGATG 3 cut(s) 36, 828, 864
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 2 cut(s) 136, 375
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 2 cut(s) 133, 372
BstMWI GCNNNNNNNGC 2 cut(s) 620, 842
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 2 cut(s) 175, 323
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 837
BstV1I GCAGC 1 cut(s) 635
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 36, 828, 864
Cac8I GCNNGC 1 cut(s) 173
CfoI GCGC 1 cut(s) 649
CseI GACGC 1 cut(s) 707
Csp6I GTAC 4 cut(s) 413, 434, 539, 670
CviAII CATG 2 cut(s) 172, 320
CviQI GTAC 4 cut(s) 413, 434, 539, 670
DdeI CTNAG 2 cut(s) 358, 641
DpnI GATC 2 cut(s) 135, 374
DpnII GATC 2 cut(s) 133, 372
EaeI YGGCCR 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 477
Eco72I CACGTG 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 477
FaeI CATG 2 cut(s) 175, 323
FaqI GGGAC 1 cut(s) 288
FatI CATG 2 cut(s) 171, 319
Fnu4HI GCNGC 1 cut(s) 624
FokI GGATG 3 cut(s) 43, 835, 871
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 1 cut(s) 624
GlaI GCGC 1 cut(s) 648
GluI GCNGC 1 cut(s) 624
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 1 cut(s) 117
HapII CCGG 3 cut(s) 291, 378, 746
HgaI GACGC 1 cut(s) 707
HhaI GCGC 1 cut(s) 649
Hin1II CATG 2 cut(s) 175, 323
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 4 cut(s) 107, 188, 397, 598
HpaII CCGG 3 cut(s) 291, 378, 746
HphI GGTGA 4 cut(s) 259, 280, 367, 404
Hpy166II GTNNAC 2 cut(s) 54, 307
Hpy188I TCNGA 3 cut(s) 361, 603, 732
Hpy188III TCNNGA 3 cut(s) 137, 569, 746
Hpy8I GTNNAC 2 cut(s) 54, 307
HpyAV CCTTC 4 cut(s) 134, 238, 544, 598
HpyCH4III ACNGT 2 cut(s) 286, 412
HpyCH4IV ACGT 2 cut(s) 70, 309
HpyCH4V TGCA 4 cut(s) 34, 171, 194, 794
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 842
HpyF3I CTNAG 2 cut(s) 358, 641
HpySE526I ACGT 2 cut(s) 70, 309
Hsp92II CATG 2 cut(s) 175, 323
HspAI GCGC 1 cut(s) 647
Kpn2I TCCGGA 1 cut(s) 745
Kzo9I GATC 2 cut(s) 133, 372
LmnI GCTCC 4 cut(s) 472, 499, 526, 553
Lsp1109I GCAGC 1 cut(s) 635
LweI GCATC 1 cut(s) 21
MaeII ACGT 2 cut(s) 70, 309
MaeIII GTNAC 2 cut(s) 286, 406
MalI GATC 2 cut(s) 135, 374
MboI GATC 2 cut(s) 133, 372
MboII GAAGA 4 cut(s) 30, 88, 116, 782
MhlI GDGCHC 1 cut(s) 477
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 2 cut(s) 337, 483
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 7 cut(s) 8, 97, 265, 306, 325, 760, 793
Mox20I TGGCCA 1 cut(s) 117
MroI TCCGGA 1 cut(s) 745
MroXI GAANNNNTTC 4 cut(s) 144, 339, 564, 615
MscI TGGCCA 1 cut(s) 117
MseI TTAA 3 cut(s) 159, 216, 863
MslI CAYNNNNRTG 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 117
MspI CCGG 3 cut(s) 291, 378, 746
MspR9I CCNGG 1 cut(s) 87
Mva1269I GAATGC 2 cut(s) 194, 845
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 620, 842
NdeII GATC 2 cut(s) 133, 372
NlaIII CATG 2 cut(s) 175, 323
NlaIV GGNNCC 5 cut(s) 450, 474, 501, 528, 555
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 2 cut(s) 175, 323
OliI CACNNNNGTG 1 cut(s) 71
PaeI GCATGC 1 cut(s) 175
PciI ACATGT 1 cut(s) 319
PctI GAATGC 2 cut(s) 194, 845
PdmI GAANNNNTTC 4 cut(s) 144, 339, 564, 615
PfeI GAWTC 3 cut(s) 188, 397, 598
PkrI GCNGC 1 cut(s) 625
PleI GAGTC 1 cut(s) 101
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 1 cut(s) 71
PscI ACATGT 1 cut(s) 319
PsiI TTATAA 1 cut(s) 830
Psp6I CCWGG 1 cut(s) 85
PspCI CACGTG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 5 cut(s) 450, 474, 501, 528, 555
RsaI GTAC 4 cut(s) 414, 435, 540, 671
RsaNI GTAC 4 cut(s) 413, 434, 539, 670
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 3 cut(s) 159, 216, 863
SatI GCNGC 1 cut(s) 624
Sau3AI GATC 2 cut(s) 133, 372
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 477
SfaNI GCATC 1 cut(s) 21
SfcI CTRYAG 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 71
SphI GCATGC 1 cut(s) 175
Sse9I AATT 2 cut(s) 337, 483
StyD4I CCNGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 286, 412
TaiI ACGT 2 cut(s) 73, 312
TaqI TCGA 3 cut(s) 132, 366, 609
TasI AATT 2 cut(s) 337, 483
TatI WGTACW 3 cut(s) 433, 538, 669
TfiI GAWTC 3 cut(s) 188, 397, 598
Tru1I TTAA 3 cut(s) 159, 216, 863
Tru9I TTAA 3 cut(s) 159, 216, 863
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 623
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 137
TspGWI ACGGA 1 cut(s) 772
XapI RAATTY 1 cut(s) 337
XceI RCATGY 2 cut(s) 175, 323
XmnI GAANNNNTTC 4 cut(s) 144, 339, 564, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.