pycom06g16720

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013677
Physical Location & Seq
Reverse (-)
20904747 .. 20905655
909 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g16720.2

Sequence Viewer

Length: 717 bp
ATGGCAGATATCAGAGGATCAATGAGAGCTATGGGAGAGAGATTACTGCCATCGACACCCACACCCATGCGCGAAAAGATTGTTCCTTCCTCAGACTGGACAGAAGACTCCAATGGCCATTACCTTCTCGTTGATCTTCCAGATTTTAAAAAGGAGGAGGTCAGGCTTGGGGTGAATATCTCAGCTGGCCACATAACAATCAGCGGAGAAAGGCAAGTGAACGGAAAGAAAACCGAGTATTTTGAGCAGAATTTTACCCTTCCACCAAACTCAGATGTTGATAAGATCACCGGAAAATTTGACGGTGAGATTCTCTATGTCACTGTACCAACGGTGGCAACAGCGGTCGAAGAACAAAGGAAAGAACCTGAAACAGAGTATGAAAATGTCCAAGGCGGCACCGCTGCTGTCACGGAACCTCCTCAAATAGAGAAGAATGAAAATGTGAAAGGCTTAAGACCCAAAAATGAAGGAGAGCATGGCTCAGACAAAGTTGATGGCATAAAGTATGGAGAGATAAGAAAATTATTAGGAGAAGGAAATAGGCACATATTCTTTTCAGCGGAAGATATAAGGAAATGGGAGGAGGAAGGAGGGAATATTATAAGGACTGCAATGGAGATGTTGAACAAAAACAAAGGGATAGTTATGACTGCTGTGGTTGCATTTTCATTTGGGATGCTAGTTTCTCGTAAGTTCCAATCAGCCGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

239

Amino Acids

26.53

Weight (kDa)

5.26

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013677)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15440
malus_domestica MD06G1188500.v1.1 MD14G1194900.v1.1 MD14G1195100.v1.1
prunus_persica Prupe.5G186800_v2.0.a1
pyrus_communis pycom06g16720 pycom14g16190
rosa_chinensis RchiOBHm_Chr7g0180641
rosa_laevigata RLG00000005247
rosa_multiflora Rmu_sc0025582.1_g000001 Rmu_sc0029934.1_g000001
rosa_roxburghii Rroxscaffold_3G00272710
rosa_rugosa Rorug06G0434600
rosa_samantha Rh7AG035800 Rh7BG035700 Rh7CG037400 Rh7DG036000
rosa_wichuraiana Rw7G002950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 605
AccB1I GGYRCC 1 cut(s) 398
AccII CGCG 1 cut(s) 72
AciI CCGC 5 cut(s) 204, 344, 396, 402, 563
AclWI GGATC 1 cut(s) 25
AcoI YGGCCR 2 cut(s) 115, 187
AcsI RAATTY 2 cut(s) 250, 296
AfaI GTAC 1 cut(s) 327
AfiI CCNNNNNNNGG 1 cut(s) 96
AflII CTTAAG 1 cut(s) 454
AgsI TTSAA 1 cut(s) 628
AluBI AGCT 2 cut(s) 29, 185
AluI AGCT 2 cut(s) 29, 185
AlwI GGATC 1 cut(s) 25
AoxI GGCC 2 cut(s) 115, 187
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 2 cut(s) 250, 296
AspLEI GCGC 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 184, 280, 317
BalI TGGCCA 2 cut(s) 117, 189
BanI GGYRCC 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 391
BccI CCATC 2 cut(s) 58, 491
BfaI CTAG 1 cut(s) 683
BfrI CTTAAG 1 cut(s) 454
BisI GCNGC 2 cut(s) 397, 405
BlsI GCNGC 2 cut(s) 398, 406
BmiI GGNNCC 2 cut(s) 400, 417
BmsI GCATC 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BsaJI CCNNGG 1 cut(s) 391
BsaWI WCCGGW 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 621
BseDI CCNNGG 1 cut(s) 391
BseGI GGATG 1 cut(s) 684
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 4 cut(s) 105, 195, 285, 498
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 3 cut(s) 170, 411, 599
BseXI GCAGC 1 cut(s) 391
Bsh1236I CGCG 1 cut(s) 72
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 117, 189
BshNI GGYRCC 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 348
BsiSI CCGG 2 cut(s) 291, 708
BslI CCNNNNNNNGG 1 cut(s) 96
BsnI GGCC 2 cut(s) 117, 189
Bsp143I GATC 3 cut(s) 17, 133, 285
BspACI CCGC 5 cut(s) 204, 344, 396, 402, 563
BspANI GGCC 2 cut(s) 117, 189
BspCNI CTCAG 4 cut(s) 104, 194, 284, 497
BspFNI CGCG 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 400, 417
BspPI GGATC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 398
BspTI CTTAAG 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 621
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 391
BssMI GATC 3 cut(s) 17, 133, 285
BssT1I CCWWGG 1 cut(s) 391
Bst4CI ACNGT 3 cut(s) 305, 325, 334
BstAFI CTTAAG 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 4 cut(s) 91, 181, 271, 484
BstF5I GGATG 1 cut(s) 684
BstFNI CGCG 1 cut(s) 72
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 3 cut(s) 20, 136, 288
BstMBI GATC 3 cut(s) 17, 133, 285
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 1 cut(s) 662
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 391
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 2 cut(s) 117, 189
BtsCI GGATG 1 cut(s) 684
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 187
CfoI GCGC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 326
CviAII CATG 2 cut(s) 67, 479
CviJI RGCY 8 cut(s) 29, 117, 166, 185, 189, 453, 483, 707
CviKI_1 RGCY 8 cut(s) 29, 117, 166, 185, 189, 453, 483, 707
CviQI GTAC 1 cut(s) 326
DdeI CTNAG 4 cut(s) 91, 181, 271, 484
DpnI GATC 3 cut(s) 19, 135, 287
DpnII GATC 3 cut(s) 17, 133, 285
DraI TTTAAA 1 cut(s) 148
EaeI YGGCCR 2 cut(s) 115, 187
Eco130I CCWWGG 1 cut(s) 391
Eco32I GATATC 1 cut(s) 10
EcoRV GATATC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 2 cut(s) 70, 482
FatI CATG 2 cut(s) 66, 478
Fnu4HI GCNGC 2 cut(s) 397, 405
FokI GGATG 1 cut(s) 691
Fsp4HI GCNGC 2 cut(s) 397, 405
FspBI CTAG 1 cut(s) 683
GlaI GCGC 1 cut(s) 71
GluI GCNGC 2 cut(s) 397, 405
HaeIII GGCC 2 cut(s) 117, 189
HapII CCGG 2 cut(s) 291, 708
HhaI GCGC 1 cut(s) 72
Hin1II CATG 2 cut(s) 70, 482
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HinfI GANTC 2 cut(s) 107, 310
HpaII CCGG 2 cut(s) 291, 708
HphI GGTGA 3 cut(s) 184, 280, 317
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 4 cut(s) 14, 94, 274, 487
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 6 cut(s) 96, 134, 269, 464, 530, 584
HpyCH4III ACNGT 3 cut(s) 305, 325, 334
HpyCH4V TGCA 2 cut(s) 614, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 662
HpyF3I CTNAG 4 cut(s) 91, 181, 271, 484
Hsp92II CATG 2 cut(s) 70, 482
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 3 cut(s) 17, 133, 285
LpnPI CCDG 6 cut(s) 82, 148, 153, 171, 304, 381
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 1 cut(s) 669
MaeI CTAG 1 cut(s) 683
MaeIII GTNAC 2 cut(s) 319, 409
MalI GATC 3 cut(s) 19, 135, 287
MboI GATC 3 cut(s) 17, 133, 285
MboII GAAGA 5 cut(s) 116, 128, 362, 445, 578
MlsI TGGCCA 2 cut(s) 117, 189
MluCI AATT 3 cut(s) 250, 296, 524
MluNI TGGCCA 2 cut(s) 117, 189
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 9 cut(s) 8, 100, 148, 151, 429, 432, 577, 580, 587
Mox20I TGGCCA 2 cut(s) 117, 189
MscI TGGCCA 2 cut(s) 117, 189
MseI TTAA 2 cut(s) 147, 455
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 2 cut(s) 117, 189
MspA1I CMGCKG 5 cut(s) 185, 204, 344, 404, 563
MspCI CTTAAG 1 cut(s) 454
MspI CCGG 2 cut(s) 291, 708
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 662
NdeII GATC 3 cut(s) 17, 133, 285
NlaIII CATG 2 cut(s) 70, 482
NlaIV GGNNCC 2 cut(s) 400, 417
NmuCI GTSAC 2 cut(s) 319, 409
PfeI GAWTC 1 cut(s) 310
PkrI GCNGC 2 cut(s) 398, 406
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PsiI TTATAA 1 cut(s) 605
PspN4I GGNNCC 2 cut(s) 400, 417
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 1 cut(s) 327
RsaNI GTAC 1 cut(s) 326
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 2 cut(s) 147, 455
SatI GCNGC 2 cut(s) 397, 405
Sau3AI GATC 3 cut(s) 17, 133, 285
SchI GAGTC 1 cut(s) 101
SetI ASST 6 cut(s) 31, 126, 162, 187, 370, 421
SfaNI GCATC 1 cut(s) 669
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse9I AATT 3 cut(s) 250, 296, 524
SsiI CCGC 5 cut(s) 204, 344, 396, 402, 563
SspI AATATT 1 cut(s) 601
SspMI CTAG 1 cut(s) 683
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 3 cut(s) 305, 325, 334
TaqI TCGA 2 cut(s) 53, 348
TasI AATT 3 cut(s) 250, 296, 524
TauI GCSGC 1 cut(s) 399
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 2 cut(s) 147, 455
Tru9I TTAA 2 cut(s) 147, 455
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 2 cut(s) 319, 409
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 2 cut(s) 319, 409
TspDTI ATGAA 4 cut(s) 396, 453, 483, 660
TspGWI ACGGA 2 cut(s) 237, 428
TspRI CASTG 1 cut(s) 328
Vha464I CTTAAG 1 cut(s) 454
XapI RAATTY 2 cut(s) 250, 296
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.