FvH4_5g16590

Leucine-rich repeat receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008490
Physical Location & Seq
Forward (+)
9452454 .. 9454439
1986 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16590.t1

Sequence Viewer

Length: 1854 bp
ATGCTTGCCTGGTTTAGCTCACAGCTCACAATGGCAAGGTATGGCCACAGCCACAACACTTCTATTTACTTCTGTTTCTTCTTGTTCTGTAATCCTCTTATTCCTATGGCCTCTGAACTATCCTCAACCCAAAAAACTACCATGATCAATATCTCCAAGAGTCTAAACAGTACTGGTAGCAGCGTTTCATGGAACATAAACAGTGAACCAAACCCATGTTTATGGAAAGGAGTCGAGTGTAATCCACCCAACTCATCTGTAACCCAAATTTCTCTCTCCAGGTTTTCTCTGTCTTCCTCAGATTTTCTCCCAATTGTCTGCCAACTTGAGTCTTTGCAGATTGTTGATGTGTCAGAAAACCATCTGAGAACAATCCCATCTGGGTTTCTCACTGCTTGTGGAAAAATAGATGGGCTAAAGCTACTCAATTTCAGTTTTAACAATCTGGTCGGTTCTTTACCTGCCTTTGTTGGGTTCTCTGGGTTGGAGTCACTGGACCTTTCTCATAATAACTTGAGTCAAGGCATTGACTCAAAGTTGGGTGGTTTGGTTGTGCTCAGAAGCTTGAACCTCAGCTTCAACTACTTTAGTGGGACTTTTCCTACCCAACTTGGGAAATCCATGCTCTTGGAGAAGCTTGTGCTTTCTGCCAATAAATTTCAAGGGATAATTCCAGGTGAAATCGTGGACTATCGCAATCTGACTCTGATTGATTTGAGTGCAAATCATCTTTCTGGTTTAGTTCCTGACAGTTTTGGAGAGCTATCCAAGTTGGAGGTTCTGATTCTATCTTCCAACAACTTAAGTGGCAAAATCCCAGAAAGCCTCTCCAACGTCACACGCCTCATGTTTTTTGCAGCAAATTCTAACAAGTTTGGGGGTCCAGTTCCTGGTGGAATTACAAAGCATCTCAAAAATTTAGACCTGAGTTATAACAGCTTCAGTGGGTTGATTCCATCAGACCTTTTGTCTCCGTTGAATTCCAACTTGCAGACTGTGGATTTGTCTAATAACTTGTTGCAGGGATCAATACCCACGCCTCTGTCTCCGGAACTGGTTGGATTGAGATTGGGAAACAATAAGCTGAATGGTTCCATACCTCCTGAATTGGGTTCATGCAGTAATTTGGCTCTGTTGAACTTGGCTCAGAATGGACTGACCGGGGCTTTGCCGCCGCAGTTGGGGAACCTCACTAAACTTCAAGTTTTGAAGCTTCAGTCTAACAATCTTGCTGGAGGAATCCCAACTGAGTTTACTGGACTAACGAGCTTGTCTGTTCTGAATATTAGCTGGAATGCTCTGAATGGTTCAATACCAACAACAGTTTCAAACTTGCAGAAGCTTATTAACATGAACATACAAGGCAACAATCTCAGTGGTTCCATCCCCAACACTATTGCCTCCATGAGTTCCCTGTTGGAACTCCAACTTGGTGACAATTTCTTGAGTGGTGACATTCCAAAGATGCCAACATCTTTGCAGATTGCTTTGAATCTCAGCAGCAACCTTTTTGAGGGACCAATTCCTAGTCATTTGTCCATGCTCACTGGATTAGAAATTTTGGATCTGTCTAACAACAAGTTCTCAGGTCAGATACCCTCATTTTTAGCTGATCAACTGGTAGCATTGACACAGTTGATACTATCTAACAATCAGCTCTCTGGAGTCTCTCCACACTTCAATACTTGGGTCATGGTCAACACAGATGGAAATGCAGGTTTAATTAAAGCTACAACACCCTCCTTGGTAAAGAAAGGAAATTCAAAGGCTTTGCCAATAGCTCTTGCAGCTGTAGGTGTTGTCGCTAGCGTAGCCATTGCCATCCTTGCTATATCACTATCACGGCATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

618

Amino Acids

65.65

Weight (kDa)

7.1

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 42 - 81 2.4e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 111 - 172 2.6e-06 Leucine rich repeat
LRR_14 PF23598 146 - 287 3.8e-07 Leucine-rich repeat region
LRR_8 PF13855 186 - 244 3.6e-07 Leucine rich repeat
LRR_8 PF13855 232 - 291 5.2e-08 Leucine rich repeat
LRR_14 PF23598 367 - 478 3.7e-09 Leucine-rich repeat region
LRR_8 PF13855 374 - 434 8.2e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
Acc36I ACCTGC 2 cut(s) 469, 1706
AccB7I CCANNNNNTGG 1 cut(s) 890
AccIII TCCGGA 1 cut(s) 1048
AciI CCGC 2 cut(s) 1172, 1175
AclWI GGATC 2 cut(s) 1033, 1572
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 7 cut(s) 267, 656, 862, 916, 979, 1557, 1759
AcuI CTGAAG 2 cut(s) 925, 1199
AfaI GTAC 1 cut(s) 172
AfiI CCNNNNNNNGG 6 cut(s) 471, 612, 890, 1109, 1181, 1513
AflII CTTAAG 1 cut(s) 802
AhdI GACNNNNNGTC 1 cut(s) 967
AjnI CCWGG 4 cut(s) 8, 278, 673, 889
AjuI GAANNNNNNNTTGG 4 cut(s) 1309, 1341, 1413, 1445
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 3 cut(s) 975, 1050, 1672
AlwI GGATC 2 cut(s) 1033, 1572
AlwNI CAGNNNCTG 1 cut(s) 890
Aor13HI TCCGGA 1 cut(s) 1048
AoxI GGCC 2 cut(s) 43, 108
ApeKI GCWGC 4 cut(s) 180, 857, 1500, 1787
ApoI RAATTY 7 cut(s) 267, 656, 862, 916, 979, 1557, 1759
ArsI GACNNNNNNTTYG 4 cut(s) 985, 1017, 1150, 1182
AspS9I GGNCC 3 cut(s) 496, 881, 1517
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 3 cut(s) 689, 1445, 1463
AsuNHI GCTAGC 1 cut(s) 1805
AvaII GGWCC 3 cut(s) 496, 881, 1517
BalI TGGCCA 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 558
BbvCI CCTCAGC 1 cut(s) 572
BbvI GCAGC 4 cut(s) 192, 869, 1512, 1799
BccI CCATC 7 cut(s) 369, 385, 404, 964, 1391, 1700, 1829
BciT130I CCWGG 4 cut(s) 10, 280, 675, 891
BclI TGATCA 2 cut(s) 144, 1612
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 3 cut(s) 975, 1050, 1672
BfaI CTAG 2 cut(s) 1527, 1806
BfmI CTRYAG 1 cut(s) 1791
BfrI CTTAAG 1 cut(s) 802
BfuAI ACCTGC 2 cut(s) 469, 1706
BisI GCNGC 6 cut(s) 181, 858, 1172, 1175, 1501, 1788
BlsI GCNGC 6 cut(s) 182, 859, 1173, 1176, 1502, 1789
BmcAI AGTACT 1 cut(s) 172
Bme1390I CCNGG 5 cut(s) 10, 280, 675, 891, 1162
Bme18I GGWCC 3 cut(s) 496, 881, 1517
BmeRI GACNNNNNGTC 1 cut(s) 967
BmgT120I GGNCC 3 cut(s) 496, 881, 1517
BmiI GGNNCC 5 cut(s) 882, 1093, 1187, 1381, 1518
BmrFI CCNGG 5 cut(s) 10, 280, 675, 891, 1162
BmsI GCATC 2 cut(s) 916, 1455
BmtI GCTAGC 1 cut(s) 1809
BpiI GAAGAC 1 cut(s) 285
BpmI CTGGAG 3 cut(s) 262, 1254, 1683
Bpu10I CCTNAGC 1 cut(s) 572
BpuEI CTTGAG 3 cut(s) 347, 535, 1465
BpuMI CCSGG 1 cut(s) 1162
BsaBI GATNNNNATC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 1161, 1743
BsaWI WCCGGW 1 cut(s) 1048
Bsc4I CCNNNNNNNGG 6 cut(s) 471, 612, 890, 1109, 1181, 1513
Bse1I ACTGG 7 cut(s) 178, 498, 884, 1059, 1261, 1552, 1623
Bse3DI GCAATG 1 cut(s) 1815
Bse8I GATNNNNATC 1 cut(s) 149
BseAI TCCGGA 1 cut(s) 1048
BseBI CCWGG 4 cut(s) 10, 280, 675, 891
BseDI CCNNGG 2 cut(s) 1161, 1743
BseGI GGATG 2 cut(s) 1383, 1821
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 6 cut(s) 471, 612, 890, 1109, 1181, 1513
BseMI GCAATG 1 cut(s) 1815
BseNI ACTGG 7 cut(s) 178, 498, 884, 1059, 1261, 1552, 1623
BseXI GCAGC 4 cut(s) 192, 869, 1512, 1799
BshFI GGCC 2 cut(s) 45, 110
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 2 cut(s) 1049, 1161
BslFI GGGAC 2 cut(s) 607, 1530
BslI CCNNNNNNNGG 6 cut(s) 471, 612, 890, 1109, 1181, 1513
BsmAI GTCTC 3 cut(s) 975, 1050, 1672
BsmFI GGGAC 2 cut(s) 607, 1530
BsmI GAATGC 1 cut(s) 1300
BsnI GGCC 2 cut(s) 45, 110
Bsp1286I GDGCHC 1 cut(s) 558
Bsp13I TCCGGA 1 cut(s) 1048
Bsp143I GATC 4 cut(s) 144, 1025, 1564, 1612
BspACI CCGC 2 cut(s) 1172, 1175
BspANI GGCC 2 cut(s) 45, 110
BspEI TCCGGA 1 cut(s) 1048
BspLI GGNNCC 5 cut(s) 882, 1093, 1187, 1381, 1518
BspMI ACCTGC 2 cut(s) 469, 1706
BspOI GCTAGC 1 cut(s) 1809
BspPI GGATC 2 cut(s) 1033, 1572
BspTI CTTAAG 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 1815
BsrI ACTGG 7 cut(s) 178, 498, 884, 1059, 1261, 1552, 1623
BssECI CCNNGG 2 cut(s) 1161, 1743
BssMI GATC 4 cut(s) 144, 1025, 1564, 1612
BssT1I CCWWGG 1 cut(s) 1743
Bst2UI CCWGG 4 cut(s) 10, 280, 675, 891
Bst4CI ACNGT 6 cut(s) 170, 203, 752, 997, 1324, 1635
BstAFI CTTAAG 1 cut(s) 802
BstC8I GCNNGC 2 cut(s) 6, 1807
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 2 cut(s) 1383, 1821
BstKTI GATC 4 cut(s) 147, 1028, 1567, 1615
BstMAI GTCTC 3 cut(s) 975, 1050, 1672
BstMBI GATC 4 cut(s) 144, 1025, 1564, 1612
BstMWI GCNNNNNNNGC 3 cut(s) 1787, 1811, 1826
BstNI CCWGG 4 cut(s) 10, 280, 675, 891
BstSCI CCNGG 5 cut(s) 8, 278, 673, 889, 1160
BstSFI CTRYAG 1 cut(s) 1791
BstV1I GCAGC 4 cut(s) 192, 869, 1512, 1799
BstV2I GAAGAC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 1564
BstXI CCANNNNNNTGG 2 cut(s) 222, 628
BstYI RGATCY 1 cut(s) 1564
BsuRI GGCC 2 cut(s) 45, 110
BtsCI GGATG 2 cut(s) 1383, 1821
BtsI GCAGTG 1 cut(s) 390
BtsIMutI CAGTG 6 cut(s) 208, 390, 491, 949, 1381, 1545
BveI ACCTGC 2 cut(s) 469, 1706
Cac8I GCNNGC 2 cut(s) 6, 1807
CaiI CAGNNNCTG 1 cut(s) 890
Cfr13I GGNCC 3 cut(s) 496, 881, 1517
Csp6I GTAC 1 cut(s) 171
CspCI CAANNNNNGTGG 4 cut(s) 787, 822, 1357, 1392
CviQI GTAC 1 cut(s) 171
DpnI GATC 4 cut(s) 146, 1027, 1566, 1614
DpnII GATC 4 cut(s) 144, 1025, 1564, 1612
DriI GACNNNNNGTC 1 cut(s) 967
EaeI YGGCCR 1 cut(s) 43
Eam1105I GACNNNNNGTC 1 cut(s) 967
Eco130I CCWWGG 1 cut(s) 1743
Eco47I GGWCC 3 cut(s) 496, 881, 1517
Eco57I CTGAAG 2 cut(s) 925, 1199
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoRI GAATTC 1 cut(s) 979
EcoRII CCWGG 4 cut(s) 8, 278, 673, 889
EcoT14I CCWWGG 1 cut(s) 1743
ErhI CCWWGG 1 cut(s) 1743
FaqI GGGAC 2 cut(s) 607, 1530
FbaI TGATCA 2 cut(s) 144, 1612
Fnu4HI GCNGC 6 cut(s) 181, 858, 1172, 1175, 1501, 1788
FokI GGATG 2 cut(s) 1370, 1808
Fsp4HI GCNGC 6 cut(s) 181, 858, 1172, 1175, 1501, 1788
FspBI CTAG 2 cut(s) 1527, 1806
GluI GCNGC 6 cut(s) 181, 858, 1172, 1175, 1501, 1788
GsuI CTGGAG 3 cut(s) 262, 1254, 1683
HaeIII GGCC 2 cut(s) 45, 110
HapII CCGG 2 cut(s) 1049, 1161
HincII GTYRAC 1 cut(s) 1699
HindII GTYRAC 1 cut(s) 1699
HindIII AAGCTT 4 cut(s) 562, 635, 1211, 1340
HpaII CCGG 2 cut(s) 1049, 1161
HphI GGTGA 3 cut(s) 689, 1445, 1463
Hpy166II GTNNAC 4 cut(s) 206, 688, 1254, 1699
Hpy188III TCNNGA 5 cut(s) 746, 1049, 1103, 1444, 1662
Hpy8I GTNNAC 4 cut(s) 206, 688, 1254, 1699
HpyCH4III ACNGT 6 cut(s) 170, 203, 752, 997, 1324, 1635
HpyCH4IV ACGT 1 cut(s) 834
HpyF10VI GCNNNNNNNGC 3 cut(s) 1787, 1811, 1826
HpySE526I ACGT 1 cut(s) 834
Kpn2I TCCGGA 1 cut(s) 1048
Ksp22I TGATCA 2 cut(s) 144, 1612
Kzo9I GATC 4 cut(s) 144, 1025, 1564, 1612
Lsp1109I GCAGC 4 cut(s) 192, 869, 1512, 1799
LweI GCATC 2 cut(s) 916, 1455
MaeI CTAG 2 cut(s) 1527, 1806
MaeII ACGT 1 cut(s) 834
MaeIII GTNAC 5 cut(s) 259, 489, 835, 1433, 1451
MalI GATC 4 cut(s) 146, 1027, 1566, 1614
MboI GATC 4 cut(s) 144, 1025, 1564, 1612
MboII GAAGA 3 cut(s) 70, 285, 783
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 1564
MhlI GDGCHC 1 cut(s) 558
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 8 cut(s) 169, 240, 338, 497, 524, 526, 697, 1674
MmeI TCCRAC 8 cut(s) 465, 753, 819, 855, 1008, 1039, 1398, 1450
Mox20I TGGCCA 1 cut(s) 45
MroI TCCGGA 1 cut(s) 1048
MscI TGGCCA 1 cut(s) 45
MseI TTAA 5 cut(s) 438, 803, 1347, 1721, 1725
MslI CAYNNNNRTG 1 cut(s) 220
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 1790
MspCI CTTAAG 1 cut(s) 802
MspI CCGG 2 cut(s) 1049, 1161
MspR9I CCNGG 5 cut(s) 10, 280, 675, 891, 1162
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 1300
MvaI CCWGG 4 cut(s) 10, 280, 675, 891
MwoI GCNNNNNNNGC 3 cut(s) 1787, 1811, 1826
NciI CCSGG 1 cut(s) 1162
NdeII GATC 4 cut(s) 144, 1025, 1564, 1612
NheI GCTAGC 1 cut(s) 1805
NlaIV GGNNCC 5 cut(s) 882, 1093, 1187, 1381, 1518
NmuCI GTSAC 4 cut(s) 489, 835, 1433, 1451
PacI TTAATTAA 1 cut(s) 1725
PctI GAATGC 1 cut(s) 1300
PfeI GAWTC 4 cut(s) 784, 952, 1239, 1492
PflMI CCANNNNNTGG 1 cut(s) 890
PkrI GCNGC 6 cut(s) 182, 859, 1173, 1176, 1502, 1789
PleI GAGTC 8 cut(s) 168, 239, 337, 496, 524, 525, 697, 1673
PpsI GAGTC 8 cut(s) 168, 239, 337, 496, 524, 525, 697, 1673
PsiI TTATAA 1 cut(s) 933
Psp6I CCWGG 4 cut(s) 8, 278, 673, 889
PspGI CCWGG 4 cut(s) 8, 278, 673, 889
PspN4I GGNNCC 5 cut(s) 882, 1093, 1187, 1381, 1518
PspPI GGNCC 3 cut(s) 496, 881, 1517
PstNI CAGNNNCTG 1 cut(s) 890
PsuI RGATCY 1 cut(s) 1564
PvuII CAGCTG 1 cut(s) 1790
RsaI GTAC 1 cut(s) 172
RsaNI GTAC 1 cut(s) 171
RseI CAYNNNNRTG 1 cut(s) 220
SaqAI TTAA 5 cut(s) 438, 803, 1347, 1721, 1725
SatI GCNGC 6 cut(s) 181, 858, 1172, 1175, 1501, 1788
Sau3AI GATC 4 cut(s) 144, 1025, 1564, 1612
Sau96I GGNCC 3 cut(s) 496, 881, 1517
ScaI AGTACT 1 cut(s) 172
SchI GAGTC 8 cut(s) 169, 240, 338, 497, 524, 526, 697, 1674
ScrFI CCNGG 5 cut(s) 10, 280, 675, 891, 1162
SduI GDGCHC 1 cut(s) 558
SfaNI GCATC 2 cut(s) 916, 1455
SfcI CTRYAG 1 cut(s) 1791
SinI GGWCC 3 cut(s) 496, 881, 1517
SmiMI CAYNNNNRTG 1 cut(s) 220
SmlI CTYRAG 4 cut(s) 326, 514, 802, 1444
SmoI CTYRAG 4 cut(s) 326, 514, 802, 1444
SsiI CCGC 2 cut(s) 1172, 1175
SspI AATATT 1 cut(s) 1285
SspMI CTAG 2 cut(s) 1527, 1806
StyD4I CCNGG 5 cut(s) 8, 278, 673, 889, 1160
StyI CCWWGG 1 cut(s) 1743
TaaI ACNGT 6 cut(s) 170, 203, 752, 997, 1324, 1635
TaiI ACGT 1 cut(s) 837
TaqI TCGA 1 cut(s) 234
TatI WGTACW 1 cut(s) 170
TauI GCSGC 2 cut(s) 1174, 1177
TfiI GAWTC 4 cut(s) 784, 952, 1239, 1492
Tru1I TTAA 5 cut(s) 438, 803, 1347, 1721, 1725
Tru9I TTAA 5 cut(s) 438, 803, 1347, 1721, 1725
TscAI CASTG 6 cut(s) 208, 397, 498, 949, 1381, 1552
TseFI GTSAC 4 cut(s) 489, 835, 1433, 1451
TseI GCWGC 4 cut(s) 180, 857, 1500, 1787
Tsp45I GTSAC 4 cut(s) 489, 835, 1433, 1451
TspDTI ATGAA 3 cut(s) 177, 1104, 1367
TspGWI ACGGA 1 cut(s) 963
TspRI CASTG 6 cut(s) 208, 397, 498, 949, 1381, 1552
Van91I CCANNNNNTGG 1 cut(s) 890
Vha464I CTTAAG 1 cut(s) 802
VpaK11BI GGWCC 3 cut(s) 496, 881, 1517
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 7 cut(s) 267, 656, 862, 916, 979, 1557, 1759
XspI CTAG 2 cut(s) 1527, 1806
ZrmI AGTACT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.