MD10G1047300.v1.1

Leucine-rich repeat receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008490
Physical Location & Seq
Reverse (-)
6295273 .. 6298577
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1047300.v1.1.491

Sequence Viewer

Length: 2892 bp
ATGAGCAGCTATGGCCGCCAAACCACCTCACTTGTCCTGTATTTCAATTTCCATTTCTTCTTGTCTTTGTTCTGCTACCTTCCTGTTCCTGTGGTGTTCTCTGCATTATCCTCAAACCAACAAACCACAATGATAGATCTCTCCAACAGCCTCAACGCTTCTGCTGGTGGTGCTGTTCCATGGGACGTCAACAAAGAACCAAACCCATGTTCGTGGAAGGGAGTCGGGTGCAATTCCCCGACCAATTCATCAGTTATCCGGATTTCGTTGTCCGGGCATTCTCTATCTTCCTCGGACTTTCTGCCGCTCGTTTGTCGGATTGAGTCTCTGCAGGTTCTTGATGTGTCCAGCAACCGTCTGACCACAATTCCGCCTGGGTTTATCTCAGCTTGTGGAAAGCTAGGTGAGTTAAAGCTGCTCAATTTTAGTCTGAATAATTTGGTGGGTCCTCTGCCCGATTTTGTTGGGTTTTTGGGTTTGGAAGCTCTGGACTTTGCTCGGAATAACTTGAGTGGAAGCATTGGCTCCAAGTTGGACGGGTTGGTCAAGCTCAGAAGCTTGAACCTTACCTTCAACCATTTTGCTGGGCATGTTCCAACCCGTCTCGGAAAGTCCAAGGTTTTGGAGGAGCTTGAGCTTTCTGTGAATACGTTTGATGGTCCTATCCCTGCTGAACTTGTGGGATATAAGAATTTGACTCTCATTGATCTTAGCGGAAATCGGCTTACCGGGATTATTCCTGCTCGGATCGGAGAGCTCTCCAAGTTGGAGGTTTTGATTCTTTCGGCCAATAACTTTTCCGGCGGAATCCCACAAAGCATTTCCAATATCACAAGCCTTTGGAGATTTGCAGCAAATTCTAACGATTTCAATGAGACGATTCCTGGTGGCATTACGGAGCATTTGAAAAACTTGGATCTCAGTTATAATAAATTGAATGGGTCGATTCCATCGGACCTCTTGTCGGCAATGAATCTGCAGACTGTGGATTTGTCGAATAATAGATTAGATGGACCGGTACCTGAAACTCTATCTCCGAGCTTGGTGAGGTTGAGACTGGGAAGCAATTCGCTTGATGGGAAGATACCTTCTGCAGCAATTGCAACACATGACAAGTTGACCTACTTGGAGATGGAAAACAATAACTTGACTGGATCAATTCCTCCTGAATTGGGTGATTGCCGGAAATTGGCGCTGCTGAATTTGGCTCAGAATCAACTGTCTGGGGCTTTGCCGGCAAAGTTGGGTCACCTTAGCAGTCTTGAGGTCTTGAAACTTCAATCGAACAATTTGAGTGGGGAAATTCCAATTGAATTTACTCGACTACCCAAGTTGTCCGTTCTGAATATCAGCTGGAATTCTATCAACGGCTCGATACCACCTTCAGTTTCAAACATGCGGAGTCTTATTAACATGAACTTACAAGGTAACAAGCTCAGCGGTTTCATTCCCGTCGGTATTGCCTCCATGGATTCTCTGATGGAACTCCAACTTGGAGAAAATCAATTAAGTGGTGACATTCCAAGAATGCCGACGAATTTACAGATTGCTTTAAATCTCAGCAGCAATCATTTTCAGGGACCTATTCCGGAAACTCTTTCGAGTCTCAGTGTATTAGAAATTCTGGATCTCTCAAATAACAAATTCTCAGGTAAGATACCTGATTTTTTGACTAAATTGGGAACCTTGACACAGTTGTCTCTTTCTAACAATGAGCTCTCTGGAGAAATTCCGACGTTCTCTTCTTGGGTCATGGTCGACACAAATGGGAACAAGGATTTGCATAACAGAACAATAAACACTCCACCCCAATTGGAGAAGAAGAAAAAATCATATGCAGCGACAATTGTTCTTGCCGTTGTAGCAGCAGCTGTGGTTGCTTTTGGAGCGATCACCATTGTTGCCGCATCAATATCACGGCAAAATTACAGGGTTAATGATGAACAAGTGCAAACAGAGGAAGAACTTGCTGTTCCTGAGGTCCTCCAGGGCAATCTATTAACTGCAAATGGTATTCATAGATCAAACATCGATTTCACTAGAGCGATGGAAGTAGTTTCCGACCAGTCAAACGTTGTGCTGAAGACTAGGTTTTCAACTTACTACAAAGCCATCATGCCGTCTGGAGCAAGCTACTTTGTGAAGAAGCTCAACTGGAGTGACAAGATATTCCAACTGGGCAGCCACGATAGGTTCGCCCGAGATTTAGAGGTCTTCGGGAAACTAAGCAATTCCAATGTCATGAATCCTCTGGCCTATGTCTTGACTGGTGACAGTGCTTATCTGTTCTATGAATTTGCTCCAAAAGGAACCCTTTTTGATGCTCTTCGTGGTATCTCGGGCGATGACATGGATTGGGGGAGCCGGTATAGTATTGCAGTTGGAGTAGCGCAGGGTCTTTCTTTTCTTCATGGGTGCACTCCAAGTCCAATACTCCTCCTTGACTTGTCAAGCAAAAGCATTTTGCTCAAGTCCCTCAAGGAGCCTTTGATTGCAGATGCTGAGCTCTTGAAGGTGATCGATCCTTCAAAGAGCACCGGAAGCCTTTCTACTATTGCTGGTTCTGTTGGCTACATTCCTCCAGAGTATGCATACACTATGGTTGTAACAATGGCCGGGAATATCTACAGCTTCGGGGTCGTTCTGCTGGAGTTGCTGACGGGAAAGCCAGCAGTTAGTGAGGGGATTGAGTTGGCCAAGTGGGTCTTGAGTAACTCAGCGCAGCAAGATAAAAGGGATCACATTCTTGATTACAGTATCAGCAGAACATCAACTGCTGTCCGAAACCAGATGCTTGCGGTCCTGAAAATTGCTCTTGCTTGTGTTAGTGTATCACCCGAAGCAAGGCCAAGAATGAAGAGTGTGCTTACGAGGCTCCTCAATGCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

964

Amino Acids

104.13

Weight (kDa)

6.53

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 389 - 500 3.4e-08 Leucine-rich repeat region
LRR_8 PF13855 395 - 455 3.7e-07 Leucine rich repeat
LRR_8 PF13855 518 - 573 4e-06 Leucine rich repeat
Pkinase PF00069 698 - 956 4.2e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 727 - 959 5.3e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 927
AasI GACNNNNNNGTC 2 cut(s) 542, 1696
AatII GACGTC 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 322
Acc65I GGTACC 1 cut(s) 1018
AccB1I GGYRCC 1 cut(s) 1018
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 1758
AccIII TCCGGA 2 cut(s) 258, 1588
AciI CCGC 9 cut(s) 16, 305, 371, 714, 804, 1399, 1440, 1905, 2798
AclI AACGTT 1 cut(s) 2073
AclWI GGATC 6 cut(s) 755, 924, 1162, 1635, 2516, 2745
AcoI YGGCCR 4 cut(s) 13, 786, 2613, 2694
AcuI CTGAAG 2 cut(s) 1368, 2102
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 1020
AfiI CCNNNNNNNGG 4 cut(s) 964, 1172, 1189, 2844
AgeI ACCGGT 1 cut(s) 1015
AhdI GACNNNNNGTC 1 cut(s) 961
AjnI CCWGG 3 cut(s) 373, 883, 1986
AjuI GAANNNNNNNTTGG 4 cut(s) 193, 225, 1476, 1508
Alw21I GWGCWC 5 cut(s) 759, 1719, 2420, 2508, 2537
Alw26I GTCTC 6 cut(s) 330, 608, 869, 1048, 1610, 1704
Alw44I GTGCAC 1 cut(s) 2416
AlwI GGATC 6 cut(s) 755, 924, 1162, 1635, 2516, 2745
AlwNI CAGNNNCTG 2 cut(s) 1871, 2501
Ama87I CYCGRG 2 cut(s) 2199, 2338
Aor13HI TCCGGA 2 cut(s) 258, 1588
AoxI GGCC 6 cut(s) 13, 786, 2253, 2613, 2694, 2846
ApaLI GTGCAC 1 cut(s) 2416
ArsI GACNNNNNNTTYG 2 cut(s) 973, 1005
AsiGI ACCGGT 1 cut(s) 1015
Asp700I GAANNNNTTC 2 cut(s) 1066, 2545
Asp718I GGTACC 1 cut(s) 1018
AspLEI GCGC 3 cut(s) 1195, 2392, 2722
AspS9I GGNCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
AsuC2I CCSGG 3 cut(s) 274, 730, 2617
AsuHPI GGTGA 9 cut(s) 416, 1057, 1187, 1241, 1526, 1885, 2282, 2527, 2826
AvaI CYCGRG 2 cut(s) 2199, 2338
AvaII GGWCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
AxyI CCTNAGG 1 cut(s) 1977
BaeGI GKGCMC 1 cut(s) 2420
BalI TGGCCA 1 cut(s) 2696
BanI GGYRCC 1 cut(s) 1018
BanII GRGCYC 3 cut(s) 759, 1719, 2508
BarI GAAGNNNNNNTAC 2 cut(s) 2533, 2565
BbsI GAAGAC 2 cut(s) 2090, 2206
Bbv12I GWGCWC 5 cut(s) 759, 1719, 2420, 2508, 2537
BccI CCATC 8 cut(s) 650, 958, 1004, 1070, 1126, 1474, 2041, 2120
BceAI ACGGC 4 cut(s) 1384, 1841, 1934, 2104
BciT130I CCWGG 3 cut(s) 375, 885, 1988
BcnI CCSGG 3 cut(s) 274, 730, 2617
BcoDI GTCTC 6 cut(s) 330, 608, 869, 1048, 1610, 1704
BfaI CTAG 3 cut(s) 401, 2040, 2088
BfmI CTRYAG 4 cut(s) 329, 977, 1092, 2626
BfoI RGCGCY 1 cut(s) 1196
BfuAI ACCTGC 1 cut(s) 322
BglII AGATCT 1 cut(s) 136
BlpI GCTNAGC 2 cut(s) 1436, 2502
Bme1390I CCNGG 6 cut(s) 274, 375, 730, 885, 1988, 2617
Bme18I GGWCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
BmeRI GACNNNNNGTC 1 cut(s) 961
BmeT110I CYCGRG 2 cut(s) 2199, 2338
BmgT120I GGNCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
BmiI GGNNCC 9 cut(s) 447, 526, 1020, 1581, 1684, 2311, 2363, 2484, 2876
BmrFI CCNGG 6 cut(s) 274, 375, 730, 885, 1988, 2617
BmrI ACTGGG 2 cut(s) 1067, 2186
BmsI GCATC 4 cut(s) 1916, 2311, 2488, 2781
BmuI ACTGGG 2 cut(s) 1067, 2186
BpiI GAAGAC 2 cut(s) 2090, 2206
BpmI CTGGAG 6 cut(s) 1743, 1970, 2145, 2176, 2565, 2669
Bpu10I CCTNAGC 1 cut(s) 1253
Bpu1102I GCTNAGC 2 cut(s) 1436, 2502
BpuEI CTTGAG 6 cut(s) 529, 653, 1283, 2453, 2462, 2728
BpuMI CCSGG 3 cut(s) 274, 730, 2617
Bsa29I ATCGAT 2 cut(s) 2031, 2520
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 6 cut(s) 179, 291, 374, 615, 1467, 1987
BsaWI WCCGGW 4 cut(s) 258, 1015, 1588, 2537
BsaXI ACNNNNNCTCC 2 cut(s) 1018, 1048
Bsc4I CCNNNNNNNGG 4 cut(s) 964, 1172, 1189, 2844
Bse118I RCCGGY 3 cut(s) 1015, 1234, 2364
Bse1I ACTGG 6 cut(s) 1062, 1156, 2065, 2159, 2181, 2272
Bse21I CCTNAGG 1 cut(s) 1977
Bse3DI GCAATG 1 cut(s) 975
BseAI TCCGGA 2 cut(s) 258, 1588
BseBI CCWGG 3 cut(s) 375, 885, 1988
BseCI ATCGAT 2 cut(s) 2031, 2520
BseDI CCNNGG 6 cut(s) 179, 291, 374, 615, 1467, 1987
BseLI CCNNNNNNNGG 4 cut(s) 964, 1172, 1189, 2844
BseMI GCAATG 1 cut(s) 975
BseNI ACTGG 6 cut(s) 1062, 1156, 2065, 2159, 2181, 2272
BseRI GAGGAG 3 cut(s) 641, 2426, 2867
BseSI GKGCMC 1 cut(s) 2420
BseYI CCCAGC 1 cut(s) 584
BshFI GGCC 6 cut(s) 15, 788, 2255, 2615, 2696, 2848
BshNI GGYRCC 1 cut(s) 1018
BshTI ACCGGT 1 cut(s) 1015
BshVI ATCGAT 2 cut(s) 2031, 2520
BsiHKAI GWGCWC 5 cut(s) 759, 1719, 2420, 2508, 2537
BsiHKCI CYCGRG 2 cut(s) 2199, 2338
BslFI GGGAC 3 cut(s) 197, 1593, 2458
BslI CCNNNNNNNGG 4 cut(s) 964, 1172, 1189, 2844
BsmAI GTCTC 6 cut(s) 330, 608, 869, 1048, 1610, 1704
BsmBI CGTCTC 2 cut(s) 608, 869
BsmFI GGGAC 3 cut(s) 197, 1593, 2458
BsmI GAATGC 2 cut(s) 277, 1533
BsnI GGCC 6 cut(s) 15, 788, 2255, 2615, 2696, 2848
BsoBI CYCGRG 2 cut(s) 2199, 2338
Bsp1286I GDGCHC 5 cut(s) 759, 1719, 2420, 2508, 2537
Bsp13I TCCGGA 2 cut(s) 258, 1588
Bsp1720I GCTNAGC 2 cut(s) 1436, 2502
Bsp19I CCATGG 2 cut(s) 179, 1467
BspACI CCGC 9 cut(s) 16, 305, 371, 714, 804, 1399, 1440, 1905, 2798
BspANI GGCC 6 cut(s) 15, 788, 2255, 2615, 2696, 2848
BspDI ATCGAT 2 cut(s) 2031, 2520
BspEI TCCGGA 2 cut(s) 258, 1588
BspHI TCATGA 1 cut(s) 2241
BspLI GGNNCC 9 cut(s) 447, 526, 1020, 1581, 1684, 2311, 2363, 2484, 2876
BspMAI CTGCAG 3 cut(s) 333, 981, 1096
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 6 cut(s) 755, 924, 1162, 1635, 2516, 2745
BspQI GCTCTTC 1 cut(s) 2331
BspT107I GGYRCC 1 cut(s) 1018
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 975
BsrFI RCCGGY 3 cut(s) 1015, 1234, 2364
BsrI ACTGG 6 cut(s) 1062, 1156, 2065, 2159, 2181, 2272
BssAI RCCGGY 3 cut(s) 1015, 1234, 2364
BssECI CCNNGG 6 cut(s) 179, 291, 374, 615, 1467, 1987
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 179, 615, 1467
Bst2UI CCWGG 3 cut(s) 375, 885, 1988
Bst4CI ACNGT 6 cut(s) 356, 985, 1221, 1695, 2276, 2756
Bst6I CTCTTC 3 cut(s) 1747, 2331, 2852
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstC8I GCNNGC 4 cut(s) 1236, 2131, 2670, 2796
BstDSI CCRYGG 2 cut(s) 179, 1467
BstEII GGTNACC 1 cut(s) 1247
BstH2I RGCGCY 1 cut(s) 1196
BstHHI GCGC 3 cut(s) 1195, 2392, 2722
BstMAI GTCTC 6 cut(s) 330, 608, 869, 1048, 1610, 1704
BstNI CCWGG 3 cut(s) 375, 885, 1988
BstNSI RCATGY 2 cut(s) 593, 1399
BstPI GGTNACC 1 cut(s) 1247
BstSCI CCNGG 6 cut(s) 272, 373, 728, 883, 1986, 2615
BstSFI CTRYAG 4 cut(s) 329, 977, 1092, 2626
BstSLI GKGCMC 1 cut(s) 2420
BstV2I GAAGAC 2 cut(s) 2090, 2206
BstX2I RGATCY 3 cut(s) 136, 916, 1627
BstXI CCANNNNNNTGG 3 cut(s) 213, 584, 622
BstYI RGATCY 3 cut(s) 136, 916, 1627
Bsu15I ATCGAT 2 cut(s) 2031, 2520
Bsu36I CCTNAGG 1 cut(s) 1977
BsuRI GGCC 6 cut(s) 15, 788, 2255, 2615, 2696, 2848
BsuTUI ATCGAT 2 cut(s) 2031, 2520
BtgI CCRYGG 2 cut(s) 179, 1467
BtgZI GCGATG 2 cut(s) 2060, 2358
BtsIMutI CAGTG 2 cut(s) 1615, 2281
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 4 cut(s) 1236, 2131, 2670, 2796
CaiI CAGNNNCTG 2 cut(s) 1871, 2501
CciI TCATGA 1 cut(s) 2241
CfoI GCGC 3 cut(s) 1195, 2392, 2722
Cfr10I RCCGGY 3 cut(s) 1015, 1234, 2364
Cfr13I GGNCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
ClaI ATCGAT 2 cut(s) 2031, 2520
Csp6I GTAC 1 cut(s) 1019
CspAI ACCGGT 1 cut(s) 1015
CspCI CAANNNNNGTGG 6 cut(s) 13, 48, 1276, 1311, 1794, 1829
CviQI GTAC 1 cut(s) 1019
DraI TTTAAA 1 cut(s) 1554
DrdI GACNNNNNNGTC 2 cut(s) 542, 1696
DriI GACNNNNNGTC 1 cut(s) 961
DseDI GACNNNNNNGTC 2 cut(s) 542, 1696
EaeI YGGCCR 4 cut(s) 13, 786, 2613, 2694
Eam1104I CTCTTC 3 cut(s) 1747, 2331, 2852
Eam1105I GACNNNNNGTC 1 cut(s) 961
EarI CTCTTC 3 cut(s) 1747, 2331, 2852
EciI GGCGGA 2 cut(s) 360, 819
Ecl136II GAGCTC 3 cut(s) 757, 1717, 2506
Eco130I CCWWGG 3 cut(s) 179, 615, 1467
Eco24I GRGCYC 3 cut(s) 759, 1719, 2508
Eco47I GGWCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
Eco53kI GAGCTC 3 cut(s) 757, 1717, 2506
Eco57I CTGAAG 2 cut(s) 1368, 2102
Eco81I CCTNAGG 1 cut(s) 1977
Eco88I CYCGRG 2 cut(s) 2199, 2338
Eco91I GGTNACC 1 cut(s) 1247
EcoICRI GAGCTC 3 cut(s) 757, 1717, 2506
EcoO109I RGGNCCY 3 cut(s) 446, 1580, 1981
EcoO65I GGTNACC 1 cut(s) 1247
EcoRI GAATTC 1 cut(s) 1357
EcoRII CCWGG 3 cut(s) 373, 883, 1986
EcoT14I CCWWGG 3 cut(s) 179, 615, 1467
EcoT22I ATGCAT 1 cut(s) 2593
EcoT38I GRGCYC 3 cut(s) 759, 1719, 2508
ErhI CCWWGG 3 cut(s) 179, 615, 1467
Esp3I CGTCTC 2 cut(s) 608, 869
FalI AAGNNNNNCTT 4 cut(s) 2298, 2330, 2690, 2722
FaqI GGGAC 3 cut(s) 197, 1593, 2458
FauNDI CATATG 1 cut(s) 1834
FblI GTMKAC 1 cut(s) 1758
FriOI GRGCYC 3 cut(s) 759, 1719, 2508
FspBI CTAG 3 cut(s) 401, 2040, 2088
GlaI GCGC 3 cut(s) 1194, 2391, 2721
GsaI CCCAGC 1 cut(s) 588
GsuI CTGGAG 6 cut(s) 1743, 1970, 2145, 2176, 2565, 2669
HaeII RGCGCY 1 cut(s) 1196
HaeIII GGCC 6 cut(s) 15, 788, 2255, 2615, 2696, 2848
HhaI GCGC 3 cut(s) 1195, 2392, 2722
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 3 cut(s) 1193, 2390, 2720
HinP1I GCGC 3 cut(s) 1193, 2390, 2720
HincII GTYRAC 3 cut(s) 190, 1119, 1759
HindII GTYRAC 3 cut(s) 190, 1119, 1759
HindIII AAGCTT 1 cut(s) 556
HphI GGTGA 9 cut(s) 416, 1057, 1187, 1241, 1526, 1885, 2282, 2527, 2826
Hpy166II GTNNAC 4 cut(s) 190, 1119, 1759, 2418
Hpy8I GTNNAC 4 cut(s) 190, 1119, 1759, 2418
Hpy99I CGWCG 3 cut(s) 1457, 1537, 1738
HpyAV CCTTC 7 cut(s) 89, 211, 580, 1098, 1392, 2506, 2535
HpyCH4III ACNGT 6 cut(s) 356, 985, 1221, 1695, 2276, 2756
HpyCH4IV ACGT 4 cut(s) 186, 650, 1736, 2073
HpySE526I ACGT 4 cut(s) 186, 650, 1736, 2073
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 3 cut(s) 1193, 2390, 2720
Kpn2I TCCGGA 2 cut(s) 258, 1588
KpnI GGTACC 1 cut(s) 1022
KroI GCCGGC 1 cut(s) 1234
KroNI GCCGGC 1 cut(s) 1236
LguI GCTCTTC 1 cut(s) 2331
LmnI GCTCC 9 cut(s) 530, 628, 898, 1886, 2126, 2305, 2361, 2482, 2880
LweI GCATC 4 cut(s) 1916, 2311, 2488, 2781
MaeI CTAG 3 cut(s) 401, 2040, 2088
MaeII ACGT 4 cut(s) 186, 650, 1736, 2073
MaeIII GTNAC 7 cut(s) 1247, 1427, 1514, 2159, 2270, 2605, 2711
MbiI CCGCTC 1 cut(s) 307
MfeI CAATTG 4 cut(s) 1098, 1308, 1811, 1845
MflI RGATCY 3 cut(s) 136, 916, 1627
MhlI GDGCHC 5 cut(s) 759, 1719, 2420, 2508, 2537
MlsI TGGCCA 1 cut(s) 2696
MluNI TGGCCA 1 cut(s) 2696
MlyI GAGTC 5 cut(s) 231, 332, 691, 1411, 1612
Mox20I TGGCCA 1 cut(s) 2696
Mph1103I ATGCAT 1 cut(s) 2593
MroI TCCGGA 2 cut(s) 258, 1588
MroNI GCCGGC 1 cut(s) 1234
MroXI GAANNNNTTC 2 cut(s) 1066, 2545
MscI TGGCCA 1 cut(s) 2696
MseI TTAA 6 cut(s) 410, 1410, 1508, 1553, 1935, 2000
MslI CAYNNNNRTG 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 2696
MspA1I CMGCKG 3 cut(s) 1353, 1440, 1871
MspR9I CCNGG 6 cut(s) 274, 375, 730, 885, 1988, 2617
MunI CAATTG 4 cut(s) 1098, 1308, 1811, 1845
Mva1269I GAATGC 2 cut(s) 277, 1533
MvaI CCWGG 3 cut(s) 375, 885, 1988
NaeI GCCGGC 1 cut(s) 1236
NciI CCSGG 3 cut(s) 274, 730, 2617
NcoI CCATGG 2 cut(s) 179, 1467
NdeI CATATG 1 cut(s) 1834
NgoMIV GCCGGC 1 cut(s) 1234
NlaIV GGNNCC 9 cut(s) 447, 526, 1020, 1581, 1684, 2311, 2363, 2484, 2876
NmuCI GTSAC 4 cut(s) 1247, 1514, 2159, 2270
NsiI ATGCAT 1 cut(s) 2593
NspI RCATGY 2 cut(s) 593, 1399
PagI TCATGA 1 cut(s) 2241
PciSI GCTCTTC 1 cut(s) 2331
PctI GAATGC 2 cut(s) 277, 1533
PdiI GCCGGC 1 cut(s) 1236
PdmI GAANNNNTTC 2 cut(s) 1066, 2545
PfeI GAWTC 8 cut(s) 778, 807, 880, 946, 973, 1213, 1472, 2245
PinAI ACCGGT 1 cut(s) 1015
PleI GAGTC 5 cut(s) 230, 331, 691, 1410, 1611
PpsI GAGTC 5 cut(s) 230, 331, 691, 1410, 1611
PpuMI RGGWCCY 3 cut(s) 446, 1580, 1981
PsiI TTATAA 1 cut(s) 927
Psp124BI GAGCTC 3 cut(s) 759, 1719, 2508
Psp1406I AACGTT 1 cut(s) 2073
Psp5II RGGWCCY 3 cut(s) 446, 1580, 1981
Psp6I CCWGG 3 cut(s) 373, 883, 1986
PspEI GGTNACC 1 cut(s) 1247
PspFI CCCAGC 1 cut(s) 584
PspGI CCWGG 3 cut(s) 373, 883, 1986
PspN4I GGNNCC 9 cut(s) 447, 526, 1020, 1581, 1684, 2311, 2363, 2484, 2876
PspPI GGNCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
PspPPI RGGWCCY 3 cut(s) 446, 1580, 1981
PstI CTGCAG 3 cut(s) 333, 981, 1096
PstNI CAGNNNCTG 2 cut(s) 1871, 2501
PsuI RGATCY 3 cut(s) 136, 916, 1627
PvuII CAGCTG 2 cut(s) 1353, 1871
RsaI GTAC 1 cut(s) 1020
RsaNI GTAC 1 cut(s) 1019
RseI CAYNNNNRTG 1 cut(s) 211
SacI GAGCTC 3 cut(s) 759, 1719, 2508
SalI GTCGAC 1 cut(s) 1757
SapI GCTCTTC 1 cut(s) 2331
SaqAI TTAA 6 cut(s) 410, 1410, 1508, 1553, 1935, 2000
Sau96I GGNCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
SchI GAGTC 5 cut(s) 231, 332, 691, 1411, 1612
ScrFI CCNGG 6 cut(s) 274, 375, 730, 885, 1988, 2617
SduI GDGCHC 5 cut(s) 759, 1719, 2420, 2508, 2537
SfaNI GCATC 4 cut(s) 1916, 2311, 2488, 2781
SfcI CTRYAG 4 cut(s) 329, 977, 1092, 2626
SinI GGWCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
SmiMI CAYNNNNRTG 1 cut(s) 211
SmlI CTYRAG 6 cut(s) 508, 632, 1262, 2468, 2477, 2707
SmoI CTYRAG 6 cut(s) 508, 632, 1262, 2468, 2477, 2707
SsiI CCGC 9 cut(s) 16, 305, 371, 714, 804, 1399, 1440, 1905, 2798
SspMI CTAG 3 cut(s) 401, 2040, 2088
SstI GAGCTC 3 cut(s) 759, 1719, 2508
StyD4I CCNGG 6 cut(s) 272, 373, 728, 883, 1986, 2615
StyI CCWWGG 3 cut(s) 179, 615, 1467
TaaI ACNGT 6 cut(s) 356, 985, 1221, 1695, 2276, 2756
TaiI ACGT 4 cut(s) 189, 653, 1739, 2076
TaqI TCGA 9 cut(s) 944, 995, 1283, 1321, 1373, 1601, 1758, 2031, 2520
TauI GCSGC 3 cut(s) 18, 307, 1907
TfiI GAWTC 8 cut(s) 778, 807, 880, 946, 973, 1213, 1472, 2245
Tru1I TTAA 6 cut(s) 410, 1410, 1508, 1553, 1935, 2000
Tru9I TTAA 6 cut(s) 410, 1410, 1508, 1553, 1935, 2000
TscAI CASTG 2 cut(s) 1615, 2281
TseFI GTSAC 4 cut(s) 1247, 1514, 2159, 2270
Tsp45I GTSAC 4 cut(s) 1247, 1514, 2159, 2270
TspGWI ACGGA 2 cut(s) 911, 1327
TspRI CASTG 2 cut(s) 1615, 2281
VneI GTGCAC 1 cut(s) 2416
VpaK11BI GGWCC 7 cut(s) 446, 659, 955, 1013, 1580, 1981, 2800
XceI RCATGY 2 cut(s) 593, 1399
XmiI GTMKAC 1 cut(s) 1758
XmnI GAANNNNTTC 2 cut(s) 1066, 2545
XspI CTAG 3 cut(s) 401, 2040, 2088
ZraI GACGTC 1 cut(s) 187
Zsp2I ATGCAT 1 cut(s) 2593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.