MD05G1041000.v1.1

Leucine-rich repeat receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008490
Physical Location & Seq
Reverse (-)
6923536 .. 6926518
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1041000.v1.1.491

Sequence Viewer

Length: 2883 bp
ATGAGCAGGTATGGCCGCCAAACCACCTCCCTTGTCCTGTATTTCAACTTCCATTTCTTCTTGTCCTTGTTCTGCTACCTTCCTCTTCCTACGGTCTTCTCCCAACTCTCCTCAAACCAGAAAACCGCCATGATCAATCTCTCCAACAACCTCAACACTCCTACTAGAGTTGTTCCATGGGACGTCAACAAAGAACCAAACCCATGTTTGTGGACGGGAGTTCGGTGCAATCCCCCGACCAATTCATCCGTCATCCAGATTTCTCTGTCCGGGCATTTTCTATCTTCCTCGGACTTTCTGCCACTCGTTTGTCGGATTCAGTCTCTGCAGATTCTCGATGTGTCCGGCAACCATCTGACCACAATCCCACCTGGGTTTATCTCGGATTGTGGAAAGCTAGGCGGGTTAAAGCTGCTCAATTTTAGTATGAACAATTTGGAGGGTCGTCTTCCCGATTTTGTCGGGTTTTCTGGGTTGGAGGCTTTGGACTTTGCTCGGAATAACTTGCATGGAAGCATCGACTCCGAGTTGGACGGGTTGGTCGGGCTCAGAAGCTTGAACCTTAGCTTCAACCAATTTACTGGGTCCGTTCCGACCCGTCTCGGAAAATTCAAGGTTTTGGAGGAGCTTGAGCTTTCTATGAATACGTTTGATGGTCCTTTTCCTGCTGAACTTGTGGGATACCATAATTTGACTCTGCTTGATCTTAGCGGAAATCAGCTTTCCGGGAGTGTTCCTGATGGAATCAGAGAGCTCGCCAAGTTGGAGGTTTTGATTCTTTCGGCCAATAAGTTATCCGGCGGAATCCCACAAAGCATTTCAAATATCACCAGCCTCTGGAGATTTGCAGCAAATTCTAACAATTTCAATGGTTCAATTCCTGATGGCATTGCAGAGCATCTGAAAAACTTGGACCTCAGTTATAATAAATTGAGTGGGTCGATTCCATCGGACCTCTTGTCATCGTGGAATCTGCATACTCTGGATTTGTCGAATAATCGATTGGAGGGACAGATACCGCCAGTTCTATCTCCGAGCTTGGTCAGGTTGAGACTGGGAAGCAATTTTCTTGATGGGAAGATACCTTCTACAGCAAATGCAACAAATCAAACGTTGACCTACTTGGAGATGGAAAACAATAACTTAACTGGATCAATTCCTCCTGAATTGGGTGATTGCCAGAATTTGGCGTTGCTGAATTTGGCTCAGAATCACCTGTCTGGGGCTCTGCCGGTGGAGTTGGGTAGCCTTAGCCATCTTCAGGTCTTGAAACTTCAATTTAACAATTTGAGTGGTCGAATTCCAATTCAAATTACTCAACTGCCCAAGTTGTCCATTCTGAATATCAGCTGGAATTCTCTCAAAGGTTCAATACCTCCTTCAGTTTCAAACATGCAGAATCTTACTAACATGAACTTACAAGGCAACAAGCTGAACGGTTCCATTCCCGCCAGTATTGCCTTCATGGATTCTCTGATGGAACTCCAACTTGGAGAAAATCACTTAAGTGGTGACATTCCAAGAATGCCGATGTCTTTGCAGATTGCTTTGAATCTCAGCAGCAATCTTTTTCAAGGACCTATTCCCGAAACTCTTTCGAGGCTCAGTAATTTAGAAATTCTGGATCTCTCAAATAACAAATTCTCAGGTAAGATACCGGATTTTTTTACTGGAATGAAAACCTTGACACAGTTGTTACTTTCTAACAATGAGCTCTCTGGAGAAATTCCGGATTTCGGTTCTTGGGTCATGGTCAACACAAGTGGGAACGAGGGTGTGACTAACAGAACAAAAAGCACTCTGCCCAAATCGGAGAAGAAAAAATCATATGCAGTGACAATTGTTCTTGCAGTTGTAGCAGCTGTGGTTGCTTTTGGAGCTGTCACCACTGTTGCCTTATCACTATCGCGACAGAATGACAGGGTTAATGATGAACGAGTTCAAACAGGGGAAGAACTTGCTGTGCCCGAGGTCCTCCAGGGAAATCTCTTGACTGCAAATGGTATTCATAGATCAAACATCGTTTTCACTAGAGCGATGGAAGTAGTTTCTGACCGATCAAACATTGTGCTGAAGACTAGGTTTTCAACTTACTACAAAGCCATCATGCCGTCTGGAGCAATCTACTTTGTCAAGAAGCTCAACTTGAGTGACAAGATATTCCAACTGGGCAGCCACGAGAGGTTCGCCAAAGATTTAGAGGTCTTTGGAAAACTAAGCAATTCCAATGTCATGAATCCACTGGCCTATGTCTTGACTGTTGACAGTGCTTATCTGTTCTATGAATTTGCTCCAAAAGGAACCCTTTTTGATGCTCTTCATGGTATCTCGGGTGATGACATGGATTGGGGGAGCAGATATAGTATTGCAGTCGGAGTAGCGCAGGGTCTTTCTTTTCTTCATGGGTGCACTCCAAGTCCAATACTCCTCCTTGACCTGTCAAGCCGAAGCATTTTGCTCAAGTCCCTCAAGGAGCCTTTGATTGGAGAAGCTGAGCTCTTGAAGGTGACTGATCCGTCAAAGAGCACCGGAAGCCTTTCTACTATTGCTGGTTCTGTTGGCTACATTCCTCCAGAGTATGCATACACTATGAGGGTAACAATGGCCGGCAATATCTATAGCTTCGGGGTCATTCTGCTGGAGTTGCTGACGGGAAAGCCAGCAGTTAGCGAGGGCATTGAGTTGGCCAAGTGGGTCTTGAGTAACTCAGCGCAGCAAGATAAAAGGGATCACATTCTTGATTATAGTATCAGCAGAACATCAATTGCCGTCCGAAGCCAGATGCTTGCAGTCCTGAAAATTGCTCTCGCTTGTGTTAGTGTATCACCGGAAGCAAGGCCAAGAATGAAGAGTGTGCTTAGAATGCTCCTCAATGCAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

961

Amino Acids

104.59

Weight (kDa)

6.96

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 149 - 306 1.6e-07 Leucine-rich repeat region
LRR_14 PF23598 388 - 496 9.3e-08 Leucine-rich repeat region
LRR_8 PF13855 517 - 572 1.5e-08 Leucine rich repeat
Pkinase PF00069 695 - 954 2e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 724 - 955 6.8e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 924
AasI GACNNNNNNGTC 2 cut(s) 539, 2515
AatII GACGTC 1 cut(s) 186
AccB7I CCANNNNNTGG 2 cut(s) 837, 1186
AccII CGCG 1 cut(s) 1909
AccIII TCCGGA 1 cut(s) 1729
AciI CCGC 7 cut(s) 16, 126, 402, 711, 801, 1019, 1449
AclI AACGTT 1 cut(s) 1112
AclWI GGATC 4 cut(s) 1159, 1632, 2507, 2736
AcoI YGGCCR 4 cut(s) 13, 783, 2604, 2685
AcuI CTGAAG 3 cut(s) 1244, 1365, 2093
AcyI GRCGYC 1 cut(s) 183
AfiI CCNNNNNNNGG 7 cut(s) 837, 1169, 1186, 1222, 1261, 1736, 2483
AflII CTTAAG 1 cut(s) 1504
AhdI GACNNNNNGTC 1 cut(s) 958
AjnI CCWGG 2 cut(s) 370, 1977
AjuI GAANNNNNNNTTGG 4 cut(s) 986, 1018, 1473, 1505
AleI CACNNNNGTG 1 cut(s) 1506
Alw21I GWGCWC 5 cut(s) 756, 1716, 2411, 2499, 2528
Alw26I GTCTC 3 cut(s) 327, 605, 1045
Alw44I GTGCAC 1 cut(s) 2407
AlwI GGATC 4 cut(s) 1159, 1632, 2507, 2736
AlwNI CAGNNNCTG 2 cut(s) 325, 837
Ama87I CYCGRG 2 cut(s) 1967, 2329
Aor13HI TCCGGA 1 cut(s) 1729
AoxI GGCC 6 cut(s) 13, 783, 2244, 2604, 2685, 2837
ApaLI GTGCAC 1 cut(s) 2407
ApeKI GCWGC 6 cut(s) 412, 848, 1560, 1859, 2172, 2713
Asp700I GAANNNNTTC 2 cut(s) 1938, 2536
AspLEI GCGC 2 cut(s) 2383, 2713
AspS9I GGNCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
AsuC2I CCSGG 2 cut(s) 271, 727
AsuHPI GGTGA 8 cut(s) 820, 1184, 1205, 1523, 1876, 2345, 2518, 2817
AvaI CYCGRG 2 cut(s) 1967, 2329
AvaII GGWCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
BaeGI GKGCMC 2 cut(s) 1968, 2411
BalI TGGCCA 1 cut(s) 2687
BanII GRGCYC 5 cut(s) 549, 756, 1228, 1716, 2499
BarI GAAGNNNNNNTAC 4 cut(s) 32, 64, 2524, 2556
BauI CACGAG 1 cut(s) 2177
BbsI GAAGAC 3 cut(s) 88, 440, 2081
Bbv12I GWGCWC 5 cut(s) 756, 1716, 2411, 2499, 2528
BbvI GCAGC 6 cut(s) 399, 860, 1572, 1871, 2184, 2725
BceAI ACGGC 2 cut(s) 2095, 2753
BcgI CGANNNNNNTGC 2 cut(s) 1519, 1553
BciT130I CCWGG 2 cut(s) 372, 1979
BciVI GTATCC 1 cut(s) 674
BclI TGATCA 1 cut(s) 132
BcnI CCSGG 2 cut(s) 271, 727
BcoDI GTCTC 3 cut(s) 327, 605, 1045
BfaI CTAG 4 cut(s) 165, 398, 2031, 2079
BfmI CTRYAG 3 cut(s) 326, 1089, 2617
BfrI CTTAAG 1 cut(s) 1504
BfuI GTATCC 1 cut(s) 674
BisI GCNGC 7 cut(s) 16, 413, 849, 1561, 1860, 2173, 2714
BlpI GCTNAGC 1 cut(s) 2493
BlsI GCNGC 7 cut(s) 17, 414, 850, 1562, 1861, 2174, 2715
Bme1390I CCNGG 4 cut(s) 271, 372, 727, 1979
Bme18I GGWCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
BmeRI GACNNNNNGTC 1 cut(s) 958
BmeT110I CYCGRG 2 cut(s) 1967, 2329
BmgT120I GGNCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
BmiI GGNNCC 4 cut(s) 586, 1441, 2302, 2475
BmrFI CCNGG 4 cut(s) 271, 372, 727, 1979
BmrI ACTGGG 3 cut(s) 591, 1064, 2177
BmsI GCATC 4 cut(s) 525, 907, 2302, 2772
BmuI ACTGGG 3 cut(s) 591, 1064, 2177
BpiI GAAGAC 3 cut(s) 88, 440, 2081
BpmI CTGGAG 6 cut(s) 859, 1740, 1961, 2136, 2556, 2660
Bpu10I CCTNAGC 2 cut(s) 563, 1250
Bpu1102I GCTNAGC 1 cut(s) 2493
BpuEI CTTGAG 5 cut(s) 650, 2167, 2444, 2453, 2719
BpuMI CCSGG 2 cut(s) 271, 727
Bsa29I ATCGAT 1 cut(s) 1000
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 176, 288, 371, 1968, 1978
BsaWI WCCGGW 4 cut(s) 1657, 1729, 2528, 2827
Bsc4I CCNNNNNNNGG 7 cut(s) 837, 1169, 1186, 1222, 1261, 1736, 2483
Bse118I RCCGGY 2 cut(s) 1231, 2606
Bse1I ACTGG 8 cut(s) 586, 1022, 1059, 1153, 1452, 1675, 2172, 2247
Bse3DI GCAATG 1 cut(s) 888
BseAI TCCGGA 1 cut(s) 1729
BseBI CCWGG 2 cut(s) 372, 1979
BseCI ATCGAT 1 cut(s) 1000
BseDI CCNNGG 5 cut(s) 176, 288, 371, 1968, 1978
BseGI GGATG 2 cut(s) 245, 252
BseLI CCNNNNNNNGG 7 cut(s) 837, 1169, 1186, 1222, 1261, 1736, 2483
BseMI GCAATG 1 cut(s) 888
BseMII CTCAG 8 cut(s) 562, 931, 1220, 1570, 1618, 1659, 2484, 2721
BseNI ACTGG 8 cut(s) 586, 1022, 1059, 1153, 1452, 1675, 2172, 2247
BseRI GAGGAG 4 cut(s) 100, 638, 2417, 2858
BseSI GKGCMC 2 cut(s) 1968, 2411
BseXI GCAGC 6 cut(s) 399, 860, 1572, 1871, 2184, 2725
Bsh1236I CGCG 1 cut(s) 1909
BshFI GGCC 6 cut(s) 15, 785, 2246, 2606, 2687, 2839
BshVI ATCGAT 1 cut(s) 1000
BsiHKAI GWGCWC 5 cut(s) 756, 1716, 2411, 2499, 2528
BsiHKCI CYCGRG 2 cut(s) 1967, 2329
BslFI GGGAC 3 cut(s) 194, 1023, 2449
BslI CCNNNNNNNGG 7 cut(s) 837, 1169, 1186, 1222, 1261, 1736, 2483
BsmAI GTCTC 3 cut(s) 327, 605, 1045
BsmBI CGTCTC 1 cut(s) 605
BsmFI GGGAC 3 cut(s) 194, 1023, 2449
BsmI GAATGC 2 cut(s) 1530, 2868
BsnI GGCC 6 cut(s) 15, 785, 2246, 2606, 2687, 2839
BsoBI CYCGRG 2 cut(s) 1967, 2329
Bsp1286I GDGCHC 8 cut(s) 549, 756, 1228, 1716, 1968, 2411, 2499, 2528
Bsp13I TCCGGA 1 cut(s) 1729
Bsp143I GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
Bsp1720I GCTNAGC 1 cut(s) 2493
Bsp19I CCATGG 1 cut(s) 176
Bsp68I TCGCGA 1 cut(s) 1909
BspACI CCGC 7 cut(s) 16, 126, 402, 711, 801, 1019, 1449
BspANI GGCC 6 cut(s) 15, 785, 2246, 2606, 2687, 2839
BspCNI CTCAG 8 cut(s) 561, 930, 1219, 1569, 1617, 1658, 2485, 2720
BspDI ATCGAT 1 cut(s) 1000
BspEI TCCGGA 1 cut(s) 1729
BspFNI CGCG 1 cut(s) 1909
BspHI TCATGA 1 cut(s) 2232
BspLI GGNNCC 4 cut(s) 586, 1441, 2302, 2475
BspMAI CTGCAG 1 cut(s) 330
BspPI GGATC 4 cut(s) 1159, 1632, 2507, 2736
BspQI GCTCTTC 1 cut(s) 2322
BspTI CTTAAG 1 cut(s) 1504
BsrDI GCAATG 1 cut(s) 888
BsrFI RCCGGY 2 cut(s) 1231, 2606
BsrI ACTGG 8 cut(s) 586, 1022, 1059, 1153, 1452, 1675, 2172, 2247
BssAI RCCGGY 2 cut(s) 1231, 2606
BssECI CCNNGG 5 cut(s) 176, 288, 371, 1968, 1978
BssMI GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 2177
BssT1I CCWWGG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 2177
Bst2UI CCWGG 2 cut(s) 372, 1979
Bst4CI ACNGT 6 cut(s) 94, 1439, 1692, 1891, 2260, 2267
Bst6I CTCTTC 3 cut(s) 90, 2322, 2843
BstACI GRCGYC 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 1504
BstC8I GCNNGC 4 cut(s) 756, 2608, 2661, 2787
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 2 cut(s) 245, 252
BstFNI CGCG 1 cut(s) 1909
BstHHI GCGC 2 cut(s) 2383, 2713
BstKTI GATC 8 cut(s) 135, 706, 1154, 1627, 2015, 2060, 2515, 2731
BstMAI GTCTC 3 cut(s) 327, 605, 1045
BstMBI GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
BstMWI GCNNNNNNNGC 8 cut(s) 12, 1457, 1856, 1868, 1877, 2532, 2644, 2863
BstNI CCWGG 2 cut(s) 372, 1979
BstNSI RCATGY 1 cut(s) 1396
BstSCI CCNGG 4 cut(s) 269, 370, 725, 1977
BstSFI CTRYAG 3 cut(s) 326, 1089, 2617
BstSLI GKGCMC 2 cut(s) 1968, 2411
BstUI CGCG 1 cut(s) 1909
BstV1I GCAGC 6 cut(s) 399, 860, 1572, 1871, 2184, 2725
BstV2I GAAGAC 3 cut(s) 88, 440, 2081
BstX2I RGATCY 1 cut(s) 1624
BstXI CCANNNNNNTGG 2 cut(s) 210, 581
BstYI RGATCY 1 cut(s) 1624
Bsu15I ATCGAT 1 cut(s) 1000
BsuI GTATCC 1 cut(s) 674
BsuRI GGCC 6 cut(s) 15, 785, 2246, 2606, 2687, 2839
BsuTUI ATCGAT 1 cut(s) 1000
BtgI CCRYGG 1 cut(s) 176
BtgZI GCGATG 1 cut(s) 2051
BtsCI GGATG 2 cut(s) 245, 252
BtsI GCAGTG 1 cut(s) 1839
BtsIMutI CAGTG 4 cut(s) 1839, 1887, 2240, 2272
BtuMI TCGCGA 1 cut(s) 1909
Cac8I GCNNGC 4 cut(s) 756, 2608, 2661, 2787
CaiI CAGNNNCTG 2 cut(s) 325, 837
CciI TCATGA 1 cut(s) 2232
CfoI GCGC 2 cut(s) 2383, 2713
Cfr10I RCCGGY 2 cut(s) 1231, 2606
Cfr13I GGNCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
ClaI ATCGAT 1 cut(s) 1000
CspCI CAANNNNNGTGG 6 cut(s) 13, 48, 1273, 1308, 1744, 1779
DpnI GATC 8 cut(s) 134, 705, 1153, 1626, 2014, 2059, 2514, 2730
DpnII GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
DrdI GACNNNNNNGTC 2 cut(s) 539, 2515
DriI GACNNNNNGTC 1 cut(s) 958
DseDI GACNNNNNNGTC 2 cut(s) 539, 2515
EaeI YGGCCR 4 cut(s) 13, 783, 2604, 2685
Eam1104I CTCTTC 3 cut(s) 90, 2322, 2843
Eam1105I GACNNNNNGTC 1 cut(s) 958
EarI CTCTTC 3 cut(s) 90, 2322, 2843
EciI GGCGGA 1 cut(s) 816
Ecl136II GAGCTC 3 cut(s) 754, 1714, 2497
Eco130I CCWWGG 1 cut(s) 176
Eco24I GRGCYC 5 cut(s) 549, 756, 1228, 1716, 2499
Eco47I GGWCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
Eco53kI GAGCTC 3 cut(s) 754, 1714, 2497
Eco57I CTGAAG 3 cut(s) 1244, 1365, 2093
Eco88I CYCGRG 2 cut(s) 1967, 2329
EcoICRI GAGCTC 3 cut(s) 754, 1714, 2497
EcoO109I RGGNCCY 2 cut(s) 1577, 1972
EcoRI GAATTC 2 cut(s) 1299, 1354
EcoRII CCWGG 2 cut(s) 370, 1977
EcoT14I CCWWGG 1 cut(s) 176
EcoT22I ATGCAT 1 cut(s) 2584
EcoT38I GRGCYC 5 cut(s) 549, 756, 1228, 1716, 2499
ErhI CCWWGG 1 cut(s) 176
Esp3I CGTCTC 1 cut(s) 605
FalI AAGNNNNNCTT 6 cut(s) 2129, 2161, 2289, 2321, 2681, 2713
FaqI GGGAC 3 cut(s) 194, 1023, 2449
FauI CCCGC 2 cut(s) 395, 1456
FauNDI CATATG 1 cut(s) 1828
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 7 cut(s) 16, 413, 849, 1561, 1860, 2173, 2714
FokI GGATG 2 cut(s) 232, 239
FriOI GRGCYC 5 cut(s) 549, 756, 1228, 1716, 2499
Fsp4HI GCNGC 7 cut(s) 16, 413, 849, 1561, 1860, 2173, 2714
FspBI CTAG 4 cut(s) 165, 398, 2031, 2079
GlaI GCGC 2 cut(s) 2382, 2712
GluI GCNGC 7 cut(s) 16, 413, 849, 1561, 1860, 2173, 2714
GsuI CTGGAG 6 cut(s) 859, 1740, 1961, 2136, 2556, 2660
HaeIII GGCC 6 cut(s) 15, 785, 2246, 2606, 2687, 2839
HhaI GCGC 2 cut(s) 2383, 2713
Hin1I GRCGYC 1 cut(s) 183
Hin6I GCGC 2 cut(s) 2381, 2711
HinP1I GCGC 2 cut(s) 2381, 2711
HincII GTYRAC 4 cut(s) 187, 1116, 1756, 2263
HindII GTYRAC 4 cut(s) 187, 1116, 1756, 2263
HindIII AAGCTT 1 cut(s) 553
HphI GGTGA 8 cut(s) 820, 1184, 1205, 1523, 1876, 2345, 2518, 2817
Hpy166II GTNNAC 6 cut(s) 187, 213, 1116, 1756, 2263, 2409
Hpy8I GTNNAC 6 cut(s) 187, 213, 1116, 1756, 2263, 2409
HpyAV CCTTC 5 cut(s) 89, 1095, 1389, 1471, 2497
HpyCH4III ACNGT 6 cut(s) 94, 1439, 1692, 1891, 2260, 2267
HpyCH4IV ACGT 3 cut(s) 183, 647, 1112
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 1457, 1856, 1868, 1877, 2532, 2644, 2863
HpySE526I ACGT 3 cut(s) 183, 647, 1112
Hsp92I GRCGYC 1 cut(s) 183
HspAI GCGC 2 cut(s) 2381, 2711
Kpn2I TCCGGA 1 cut(s) 1729
KroI GCCGGC 1 cut(s) 2606
KroNI GCCGGC 1 cut(s) 2608
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
LguI GCTCTTC 1 cut(s) 2322
LmnI GCTCC 7 cut(s) 625, 1877, 2117, 2296, 2352, 2473, 2871
Lsp1109I GCAGC 6 cut(s) 399, 860, 1572, 1871, 2184, 2725
LweI GCATC 4 cut(s) 525, 907, 2302, 2772
MaeI CTAG 4 cut(s) 165, 398, 2031, 2079
MaeII ACGT 3 cut(s) 183, 647, 1112
MaeIII GTNAC 9 cut(s) 1511, 1695, 1777, 1834, 1882, 2150, 2506, 2596, 2702
MalI GATC 8 cut(s) 134, 705, 1153, 1626, 2014, 2059, 2514, 2730
MboI GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
MfeI CAATTG 2 cut(s) 1839, 2763
MflI RGATCY 1 cut(s) 1624
MhlI GDGCHC 8 cut(s) 549, 756, 1228, 1716, 1968, 2411, 2499, 2528
MlsI TGGCCA 1 cut(s) 2687
MluNI TGGCCA 1 cut(s) 2687
MlyI GAGTC 2 cut(s) 515, 688
MmeI TCCRAC 9 cut(s) 168, 293, 456, 510, 617, 744, 1510, 2188, 2353
Mox20I TGGCCA 1 cut(s) 2687
Mph1103I ATGCAT 1 cut(s) 2584
MroI TCCGGA 1 cut(s) 1729
MroNI GCCGGC 1 cut(s) 2606
MroXI GAANNNNTTC 2 cut(s) 1938, 2536
MscI TGGCCA 1 cut(s) 2687
MseI TTAA 5 cut(s) 407, 1145, 1281, 1505, 1926
MslI CAYNNNNRTG 2 cut(s) 208, 1506
Msp20I TGGCCA 1 cut(s) 2687
MspA1I CMGCKG 2 cut(s) 1350, 1862
MspCI CTTAAG 1 cut(s) 1504
MspR9I CCNGG 4 cut(s) 271, 372, 727, 1979
MunI CAATTG 2 cut(s) 1839, 2763
Mva1269I GAATGC 2 cut(s) 1530, 2868
MvaI CCWGG 2 cut(s) 372, 1979
MvnI CGCG 1 cut(s) 1909
MwoI GCNNNNNNNGC 8 cut(s) 12, 1457, 1856, 1868, 1877, 2532, 2644, 2863
NaeI GCCGGC 1 cut(s) 2608
NciI CCSGG 2 cut(s) 271, 727
NcoI CCATGG 1 cut(s) 176
NdeI CATATG 1 cut(s) 1828
NdeII GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
NgoMIV GCCGGC 1 cut(s) 2606
NlaIV GGNNCC 4 cut(s) 586, 1441, 2302, 2475
NmuCI GTSAC 6 cut(s) 1511, 1777, 1834, 1882, 2150, 2506
NruI TCGCGA 1 cut(s) 1909
NsiI ATGCAT 1 cut(s) 2584
NspI RCATGY 1 cut(s) 1396
OliI CACNNNNGTG 1 cut(s) 1506
PagI TCATGA 1 cut(s) 2232
PciSI GCTCTTC 1 cut(s) 2322
PctI GAATGC 2 cut(s) 1530, 2868
PdiI GCCGGC 1 cut(s) 2608
PdmI GAANNNNTTC 2 cut(s) 1938, 2536
PflMI CCANNNNNTGG 2 cut(s) 837, 1186
PfoI TCCNGGA 1 cut(s) 725
PkrI GCNGC 7 cut(s) 17, 414, 850, 1562, 1861, 2174, 2715
PleI GAGTC 2 cut(s) 515, 688
PpsI GAGTC 2 cut(s) 515, 688
PpuMI RGGWCCY 2 cut(s) 1577, 1972
PsiI TTATAA 1 cut(s) 924
Psp124BI GAGCTC 3 cut(s) 756, 1716, 2499
Psp1406I AACGTT 1 cut(s) 1112
Psp5II RGGWCCY 2 cut(s) 1577, 1972
Psp6I CCWGG 2 cut(s) 370, 1977
PspGI CCWGG 2 cut(s) 370, 1977
PspN4I GGNNCC 4 cut(s) 586, 1441, 2302, 2475
PspPI GGNCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
PspPPI RGGWCCY 2 cut(s) 1577, 1972
PstI CTGCAG 1 cut(s) 330
PstNI CAGNNNCTG 2 cut(s) 325, 837
PsuI RGATCY 1 cut(s) 1624
PvuII CAGCTG 2 cut(s) 1350, 1862
RruI TCGCGA 1 cut(s) 1909
RseI CAYNNNNRTG 2 cut(s) 208, 1506
SacI GAGCTC 3 cut(s) 756, 1716, 2499
SapI GCTCTTC 1 cut(s) 2322
SaqAI TTAA 5 cut(s) 407, 1145, 1281, 1505, 1926
SatI GCNGC 7 cut(s) 16, 413, 849, 1561, 1860, 2173, 2714
Sau3AI GATC 8 cut(s) 132, 703, 1151, 1624, 2012, 2057, 2512, 2728
Sau96I GGNCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
SchI GAGTC 2 cut(s) 515, 688
ScrFI CCNGG 4 cut(s) 271, 372, 727, 1979
SduI GDGCHC 8 cut(s) 549, 756, 1228, 1716, 1968, 2411, 2499, 2528
SfaNI GCATC 4 cut(s) 525, 907, 2302, 2772
SfcI CTRYAG 3 cut(s) 326, 1089, 2617
SinI GGWCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
SmiMI CAYNNNNRTG 2 cut(s) 208, 1506
SmlI CTYRAG 6 cut(s) 629, 1504, 2146, 2459, 2468, 2698
SmoI CTYRAG 6 cut(s) 629, 1504, 2146, 2459, 2468, 2698
SsiI CCGC 7 cut(s) 16, 126, 402, 711, 801, 1019, 1449
SspMI CTAG 4 cut(s) 165, 398, 2031, 2079
SstI GAGCTC 3 cut(s) 756, 1716, 2499
StyD4I CCNGG 4 cut(s) 269, 370, 725, 1977
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 6 cut(s) 94, 1439, 1692, 1891, 2260, 2267
TaiI ACGT 3 cut(s) 186, 650, 1115
TaqI TCGA 7 cut(s) 336, 519, 941, 992, 1000, 1297, 1598
TaqII GACCGA 1 cut(s) 2070
TauI GCSGC 1 cut(s) 18
Tru1I TTAA 5 cut(s) 407, 1145, 1281, 1505, 1926
Tru9I TTAA 5 cut(s) 407, 1145, 1281, 1505, 1926
TscAI CASTG 4 cut(s) 1839, 1894, 2247, 2272
TseFI GTSAC 6 cut(s) 1511, 1777, 1834, 1882, 2150, 2506
TseI GCWGC 6 cut(s) 412, 848, 1560, 1859, 2172, 2713
Tsp45I GTSAC 6 cut(s) 1511, 1777, 1834, 1882, 2150, 2506
TspGWI ACGGA 3 cut(s) 238, 577, 2505
TspRI CASTG 4 cut(s) 1839, 1894, 2247, 2272
Van91I CCANNNNNTGG 2 cut(s) 837, 1186
Vha464I CTTAAG 1 cut(s) 1504
VneI GTGCAC 1 cut(s) 2407
VpaK11BI GGWCC 6 cut(s) 585, 656, 913, 952, 1577, 1972
XceI RCATGY 1 cut(s) 1396
XmnI GAANNNNTTC 2 cut(s) 1938, 2536
XspI CTAG 4 cut(s) 165, 398, 2031, 2079
ZraI GACGTC 1 cut(s) 184
Zsp2I ATGCAT 1 cut(s) 2584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.