Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0005853

Physical Location & Seq

Genomic Coordinates Reverse (-)

9613093 .. 9618783

Gene Structure 5691 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g16820.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2196 bp

ATGGGGAGCCCCGGATTAAGCAACCGGATTAATGGCCAGCAGCGATTAGGCATTACCGAGCCGATTTCGCTGGCTGGTCCTTCAGAGGAGGATGTGATCAAAACCCGTGAGCTTGAGCAGTATTTGCATGATGCGGGATTGTATGAGAGTCAGGAAGAGGCTGTAAGTAGGGAAGAGGTGCTCGGCAGGCTTGATCAGATTGTGAAGATATGGGTTAAAACCATCAGCCGGGCGAAGGGTTTGCACGATGAATTGGTGCATCAAGCAAATGCTAAGATCTTCACATTCGGTTCATATAGGCTAGGGGTTCATGGCCCTGGTGCAGATATAGACACACTCTGTGTTGGACCCAGACACGCAAGTCGAGAAGAAGATTTCTTTGGTGAGCTATACAGGATGCTATCTGAAATGTCTGAAGTAACGGAGTTACATCCTGTTCCTGATGCCCATGTTCCTATTATGCAGTTCAAGTTTAACGGAGTTTCAATTGATCTTCTTTATGCAAAACTATCTCTCTATGTTATTCCTGAAGACTTAGATGTATCACAAGATGCAATACTACATGGTGCGGATGAACAGACAGTTCGTAGTCTCAATGGCTGTAGAGTTACGGACCAAATTCTGCGTTTAGTCCCAAGCATTCAGAATTTCCGCACAACAGTACGATGCATGAGGTTATGGGCAAAGCGTCGCGGTGTTTATTCAAATGTTGCGGGATTTCTTGGTGGCATAAACTGGGCATTGCTTGTTGCTCGCATATGCCAACTTTACCCGAATGCATTGCCTAATATGCTAGTGTCTCGGTTCTTCAGGGTCTATACCCAATGGCGTTGGCCAAATCCAGTAATGCTCTGTGCTATTGAAGAAGGGTCTCTAGGACTTCCGATTTGGGATCCCAGAAGAAATCCTAGGGACAGATATCATTTGATGCCTATAATTACTCCAGCTTATCCTTGCATGAACTCTAGCTACAATGTGTCCTCAAGTACTCTGCGTATCATGTTAGAGGAGTTTCAGAGGGGAAATGAAATTTGTGAGGAAATGGAAGCAAATAAGGCTGATTGGGATACCCTTTTTGAGTTGTATGCTTTCTTTGAAGCATATAAGCATTATTTACAGATAGACATCAGTGCAGCAAATGCTGATGGCTTTAGAAAATGGAAAGGCTGGGTTGAGTCTCGTCTCCGTCAACTAACATTGAAGATTGAGAGGAAAACTTATGAAATGCTGCAGTGTCACCCACACCCAGGTGATTTTCCAGATAAGTCCAGACCTTTTCATAGCTCATACTTCATGGGCCTACAACGAAAACAAGGAGTTCCAGTCAAGGAAGGTGAGCATTTTGATTTAAATAGAGCTGTTGATGACTTCAAGCTTGCTGTCTACCAGTATGGATTAAGAGAAATTGGAATGGAGATTCGTGTATCCCATGTAAAACGCAGGGACATCCCAAATTTTGTATTTCCTGGAGGGGTCAGGCCTTCACGCCCATCTAAAGTAAGTTGGGTTAGAAAGCGAAAACGTTCAGAAGTGGAGGTTTCTGGCAGCAATACTCAATCAGATAGTTTAGGTGAATATAGAACTGTGTCAGAGGCAGATAGTTGTCAAAAGAGAAAATTGGAAAATGATAATCTTATGCCTCCTGGCATTGGGGAAGTTTGTGAAGCTAGCCCTCCTTTTAACAATGTCAGTTCATGTGCCGCTAAATGTGAGAATAATGTAGAAATGGCCGCAGAAAAGATAGAAAGCACAGCTCACACTCCTTATCAGAATGGTCAGACTGAAGTGTGGTCAAGGTGCAATCCACTGAACAACAGTCTATCTGTTGCTGCTGATGCAACAAATTCTAAAGAAGCAGAGAAACTGGCCTTAGAGAAGATTATGGCCGGTCCATATGTTTCACATCAAGCTTTGCCAGAAGAACTGGATGAGCTCGAAGATGATTCTGAAGAAAGACATCAAGTCAAAGACTTTCCTGAGAACATGAAAGACGGTCACATCGAGCCTTCAATCGAGAAATCTAGCAATGGAGCTGAAACTTCCACTAGTTCATATAATGGAGGCTTAGAGGAACTTGAGCCTGCTGAACTTATGGCGCCATCCATGAGTGGAACCCCTGAACCTGTGGCACAGAGGAAGCCAATCATCAGGCTGAATTTCACTTCCTTGCCCAATGTGTCTGGAAAGAGTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

732

Amino Acids

82.61

Weight (kDa)

5.68

Isoelectric Point (pI)

47.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	17 - 211	1e-79	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	88 - 166	1e-10	Nucleotidyltransferase domain
PAP_central	PF04928	216 - 363	7.2e-67	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	367 - 425	2.2e-13	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17980 AT1G17980
fragaria_vesca	FvH4_5g16820 FvH4_5g16820 FvH4_5g16820 FvH4_5g16820
malus_domestica	MD06G1222200.v1.1 MD09G1233900.v1.1 MD14G1231300.v1.1 MD17G1263100.v1.1
prunus_persica	Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1
pyrus_communis	pycom06g19770 pycom14g19250 pycom17g26340
rosa_chinensis	RchiOBHm_Chr7g0179251
rosa_laevigata	RLG00000005369
rosa_multiflora	Rmu_sc0006939.1_g000005
rosa_roxburghii	Rroxscaffold_3G00274180
rosa_rugosa	Rorug06G0422700 Rorug06G0422800 Rorug06G0422800
rosa_samantha	Rh7AG022000 Rh7BG021900 Rh7CG023200 Rh7DG022500
rosa_wichuraiana	Rw7G001810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	360
AccB1I	GGYRCC	1 cut(s)	2095
AccI	GTMKAC	1 cut(s)	1383
AccII	CGCG	1 cut(s)	693
AciI	CCGC	7 cut(s)	134, 569, 652, 693, 713, 1701, 1731
AclI	AACGTT	1 cut(s)	1522
AclWI	GGATC	2 cut(s)	887, 900
AcoI	YGGCCR	4 cut(s)	34, 833, 1728, 1884
AcsI	RAATTY	6 cut(s)	618, 646, 1029, 1453, 1843, 2155
AcuI	CTGAAG	6 cut(s)	66, 435, 549, 793, 1803, 1968
AcyI	GRCGYC	1 cut(s)	2096
AdeI	CACNNNGTG	1 cut(s)	341
AfaI	GTAC	2 cut(s)	663, 988
AfiI	CCNNNNNNNGG	4 cut(s)	228, 235, 1247, 1649
AgsI	TTSAA	8 cut(s)	469, 486, 705, 863, 1097, 1201, 1372, 2010
AhdI	GACNNNNNGTC	1 cut(s)	1961
AhlI	ACTAGT	1 cut(s)	2045
AjnI	CCWGG	4 cut(s)	316, 1246, 1465, 1642
AjuI	GAANNNNNNNTTGG	2 cut(s)	363, 395
AleI	CACNNNNGTG	1 cut(s)	1248
Alw21I	GWGCWC	2 cut(s)	183, 1935
Alw26I	GTCTC	5 cut(s)	596, 804, 876, 1182, 1187
AlwI	GGATC	2 cut(s)	887, 900
AoxI	GGCC	8 cut(s)	34, 313, 833, 1297, 1478, 1728, 1866, 1884
ApeKI	GCWGC	5 cut(s)	40, 1133, 1228, 1545, 1829
ApoI	RAATTY	6 cut(s)	618, 646, 1029, 1453, 1843, 2155
ArsI	GACNNNNNNTTYG	2 cut(s)	870, 902
AseI	ATTAAT	1 cut(s)	30
Asp700I	GAANNNNTTC	2 cut(s)	1522, 2188
AspA2I	CCTAGG	1 cut(s)	908
AspLEI	GCGC	1 cut(s)	2098
AspS9I	GGNCC	6 cut(s)	77, 314, 347, 613, 1297, 1889
AsuC2I	CCSGG	2 cut(s)	12, 230
AsuHPI	GGTGA	5 cut(s)	395, 1229, 1262, 1346, 1583
AsuNHI	GCTAGC	1 cut(s)	1667
AvaII	GGWCC	4 cut(s)	77, 347, 613, 1889
AvrII	CCTAGG	1 cut(s)	908
BalI	TGGCCA	2 cut(s)	36, 835
BamHI	GGATCC	1 cut(s)	892
BanI	GGYRCC	1 cut(s)	2095
BanII	GRGCYC	2 cut(s)	11, 1935
BbsI	GAAGAC	1 cut(s)	537
Bbv12I	GWGCWC	2 cut(s)	183, 1935
BbvI	GCAGC	5 cut(s)	52, 1145, 1215, 1557, 1816
BccI	CCATC	4 cut(s)	230, 1139, 1498, 2107
BcgI	CGANNNNNNTGC	4 cut(s)	223, 257, 763, 797
BciT130I	CCWGG	4 cut(s)	318, 1248, 1467, 1644
BciVI	GTATCC	2 cut(s)	1060, 1435
BclI	TGATCA	2 cut(s)	96, 193
BcnI	CCSGG	2 cut(s)	12, 230
BcoDI	GTCTC	5 cut(s)	596, 804, 876, 1182, 1187
BcuI	ACTAGT	1 cut(s)	2045
BfaI	CTAG	8 cut(s)	302, 794, 875, 909, 966, 1668, 2022, 2046
BfmI	CTRYAG	2 cut(s)	601, 1229
BfoI	RGCGCY	1 cut(s)	2099
BfuI	GTATCC	2 cut(s)	1060, 1435
BglII	AGATCT	1 cut(s)	276
BisI	GCNGC	7 cut(s)	41, 1134, 1229, 1546, 1701, 1731, 1830
BlnI	CCTAGG	1 cut(s)	908
BlsI	GCNGC	7 cut(s)	42, 1135, 1230, 1547, 1702, 1732, 1831
BmcAI	AGTACT	1 cut(s)	988
Bme1390I	CCNGG	6 cut(s)	12, 230, 318, 1248, 1467, 1644
Bme18I	GGWCC	4 cut(s)	77, 347, 613, 1889
BmeRI	GACNNNNNGTC	1 cut(s)	1961
BmgT120I	GGNCC	6 cut(s)	77, 314, 347, 613, 1297, 1889
BmiI	GGNNCC	5 cut(s)	8, 349, 894, 2097, 2113
BmrFI	CCNGG	6 cut(s)	12, 230, 318, 1248, 1467, 1644
BmrI	ACTGGG	1 cut(s)	745
BmsI	GCATC	8 cut(s)	121, 268, 387, 433, 541, 656, 918, 1825
BmtI	GCTAGC	1 cut(s)	1671
BmuI	ACTGGG	1 cut(s)	745
BpiI	GAAGAC	1 cut(s)	537
BpmI	CTGGAG	2 cut(s)	927, 1488
BpuEI	CTTGAG	3 cut(s)	134, 967, 2096
BpuMI	CCSGG	2 cut(s)	12, 230
BsaBI	GATNNNNATC	1 cut(s)	1124
BsaHI	GRCGYC	1 cut(s)	2096
BsaI	GGTCTC	1 cut(s)	876
BsaJI	CCNNGG	4 cut(s)	10, 316, 908, 1246
BsaWI	WCCGGW	1 cut(s)	24
BsaXI	ACNNNNNCTCC	2 cut(s)	1308, 1338
Bsc4I	CCNNNNNNNGG	4 cut(s)	228, 235, 1247, 1649
Bse118I	RCCGGY	1 cut(s)	1886
Bse1I	ACTGG	6 cut(s)	740, 842, 1322, 1387, 1869, 1929
Bse3DI	GCAATG	3 cut(s)	740, 779, 2032
Bse8I	GATNNNNATC	1 cut(s)	1124
BseBI	CCWGG	4 cut(s)	318, 1248, 1467, 1644
BseDI	CCNNGG	4 cut(s)	10, 316, 908, 1246
BseGI	GGATG	7 cut(s)	97, 402, 430, 577, 1446, 1933, 2099
BseJI	GATNNNNATC	1 cut(s)	1124
BseLI	CCNNNNNNNGG	4 cut(s)	228, 235, 1247, 1649
BseMI	GCAATG	3 cut(s)	740, 779, 2032
BseMII	CTCAG	1 cut(s)	1968
BseNI	ACTGG	6 cut(s)	740, 842, 1322, 1387, 1869, 1929
BseRI	GAGGAG	2 cut(s)	101, 1022
BseXI	GCAGC	5 cut(s)	52, 1145, 1215, 1557, 1816
BseYI	CCCAGC	1 cut(s)	1167
BsgI	GTGCAG	2 cut(s)	342, 1152
Bsh1236I	CGCG	1 cut(s)	693
BshFI	GGCC	8 cut(s)	36, 315, 835, 1299, 1480, 1730, 1868, 1886
BshNI	GGYRCC	1 cut(s)	2095
BsiHKAI	GWGCWC	2 cut(s)	183, 1935
BsiSI	CCGG	4 cut(s)	12, 25, 229, 1887
BslFI	GGGAC	3 cut(s)	617, 926, 1457
BslI	CCNNNNNNNGG	4 cut(s)	228, 235, 1247, 1649
BsmAI	GTCTC	5 cut(s)	596, 804, 876, 1182, 1187
BsmBI	CGTCTC	1 cut(s)	1187
BsmFI	GGGAC	3 cut(s)	617, 926, 1457
BsmI	GAATGC	2 cut(s)	639, 781
BsnI	GGCC	8 cut(s)	36, 315, 835, 1299, 1480, 1730, 1868, 1886
Bso31I	GGTCTC	1 cut(s)	876
Bsp1286I	GDGCHC	3 cut(s)	11, 183, 1935
Bsp143I	GATC	5 cut(s)	96, 193, 276, 490, 892
BspACI	CCGC	7 cut(s)	134, 569, 652, 693, 713, 1701, 1731
BspANI	GGCC	8 cut(s)	36, 315, 835, 1299, 1480, 1730, 1868, 1886
BspCNI	CTCAG	1 cut(s)	1969
BspFNI	CGCG	1 cut(s)	693
BspLI	GGNNCC	5 cut(s)	8, 349, 894, 2097, 2113
BspMAI	CTGCAG	1 cut(s)	1233
BspOI	GCTAGC	1 cut(s)	1671
BspPI	GGATC	2 cut(s)	887, 900
BspT107I	GGYRCC	1 cut(s)	2095
BspTNI	GGTCTC	1 cut(s)	876
BsrDI	GCAATG	3 cut(s)	740, 779, 2032
BsrFI	RCCGGY	1 cut(s)	1886
BsrI	ACTGG	6 cut(s)	740, 842, 1322, 1387, 1869, 1929
BssAI	RCCGGY	1 cut(s)	1886
BssECI	CCNNGG	4 cut(s)	10, 316, 908, 1246
BssMI	GATC	5 cut(s)	96, 193, 276, 490, 892
BssNI	GRCGYC	1 cut(s)	2096
BssT1I	CCWWGG	1 cut(s)	908
Bst2UI	CCWGG	4 cut(s)	318, 1248, 1467, 1644
Bst4CI	ACNGT	5 cut(s)	583, 661, 1585, 1817, 1994
Bst6I	CTCTTC	2 cut(s)	150, 168
BstACI	GRCGYC	1 cut(s)	2096
BstAPI	GCANNNNNTGC	2 cut(s)	124, 1139
BstC8I	GCNNGC	7 cut(s)	38, 72, 188, 754, 1377, 1669, 2082
BstDEI	CTNAG	5 cut(s)	273, 535, 1870, 1977, 2065
BstF5I	GGATG	7 cut(s)	97, 402, 430, 577, 1446, 1933, 2099
BstFNI	CGCG	1 cut(s)	693
BstH2I	RGCGCY	1 cut(s)	2099
BstHHI	GCGC	1 cut(s)	2098
BstKTI	GATC	5 cut(s)	99, 196, 279, 493, 895
BstMAI	GTCTC	5 cut(s)	596, 804, 876, 1182, 1187
BstMBI	GATC	5 cut(s)	96, 193, 276, 490, 892
BstMWI	GCNNNNNNNGC	7 cut(s)	67, 124, 187, 790, 1055, 1139, 1835
BstNI	CCWGG	4 cut(s)	318, 1248, 1467, 1644
BstSCI	CCNGG	6 cut(s)	10, 228, 316, 1246, 1465, 1642
BstSFI	CTRYAG	2 cut(s)	601, 1229
BstUI	CGCG	1 cut(s)	693
BstV1I	GCAGC	5 cut(s)	52, 1145, 1215, 1557, 1816
BstV2I	GAAGAC	1 cut(s)	537
BstX2I	RGATCY	2 cut(s)	276, 892
BstYI	RGATCY	2 cut(s)	276, 892
BsuI	GTATCC	2 cut(s)	1060, 1435
BsuRI	GGCC	8 cut(s)	36, 315, 835, 1299, 1480, 1730, 1868, 1886
BtsCI	GGATG	7 cut(s)	97, 402, 430, 577, 1446, 1933, 2099
BtsI	GCAGTG	1 cut(s)	1238
BtsIMutI	CAGTG	3 cut(s)	1135, 1238, 1805
Cac8I	GCNNGC	7 cut(s)	38, 72, 188, 754, 1377, 1669, 2082
CfoI	GCGC	1 cut(s)	2098
Cfr10I	RCCGGY	1 cut(s)	1886
Cfr13I	GGNCC	6 cut(s)	77, 314, 347, 613, 1297, 1889
CseI	GACGC	1 cut(s)	677
Csp6I	GTAC	2 cut(s)	662, 987
CviQI	GTAC	2 cut(s)	662, 987
DdeI	CTNAG	5 cut(s)	273, 535, 1870, 1977, 2065
DinI	GGCGCC	1 cut(s)	2097
DpnI	GATC	5 cut(s)	98, 195, 278, 492, 894
DpnII	GATC	5 cut(s)	96, 193, 276, 490, 892
DraI	TTTAAA	1 cut(s)	1350
DraIII	CACNNNGTG	1 cut(s)	341
DrdI	GACNNNNNNGTC	1 cut(s)	360
DriI	GACNNNNNGTC	1 cut(s)	1961
DseDI	GACNNNNNNGTC	1 cut(s)	360
EaeI	YGGCCR	4 cut(s)	34, 833, 1728, 1884
Eam1104I	CTCTTC	2 cut(s)	150, 168
Eam1105I	GACNNNNNGTC	1 cut(s)	1961
EarI	CTCTTC	2 cut(s)	150, 168
Ecl136II	GAGCTC	1 cut(s)	1933
Eco130I	CCWWGG	1 cut(s)	908
Eco147I	AGGCCT	1 cut(s)	1480
Eco24I	GRGCYC	2 cut(s)	11, 1935
Eco31I	GGTCTC	1 cut(s)	876
Eco32I	GATATC	1 cut(s)	920
Eco47I	GGWCC	4 cut(s)	77, 347, 613, 1889
Eco53kI	GAGCTC	1 cut(s)	1933
Eco57I	CTGAAG	6 cut(s)	66, 435, 549, 793, 1803, 1968
EcoICRI	GAGCTC	1 cut(s)	1933
EcoRII	CCWGG	4 cut(s)	316, 1246, 1465, 1642
EcoRV	GATATC	1 cut(s)	920
EcoT14I	CCWWGG	1 cut(s)	908
EcoT22I	ATGCAT	2 cut(s)	671, 781
EcoT38I	GRGCYC	2 cut(s)	11, 1935
EgeI	GGCGCC	1 cut(s)	2097
EheI	GGCGCC	1 cut(s)	2097
ErhI	CCWWGG	1 cut(s)	908
Esp3I	CGTCTC	1 cut(s)	1187
FaqI	GGGAC	3 cut(s)	617, 926, 1457
FauI	CCCGC	2 cut(s)	127, 706
FauNDI	CATATG	2 cut(s)	758, 1894
FbaI	TGATCA	2 cut(s)	96, 193
FblI	GTMKAC	1 cut(s)	1383
Fnu4HI	GCNGC	7 cut(s)	41, 1134, 1229, 1546, 1701, 1731, 1830
FokI	GGATG	7 cut(s)	104, 409, 417, 584, 1433, 1940, 2086
FriOI	GRGCYC	2 cut(s)	11, 1935
Fsp4HI	GCNGC	7 cut(s)	41, 1134, 1229, 1546, 1701, 1731, 1830
FspBI	CTAG	8 cut(s)	302, 794, 875, 909, 966, 1668, 2022, 2046
GlaI	GCGC	1 cut(s)	2097
GluI	GCNGC	7 cut(s)	41, 1134, 1229, 1546, 1701, 1731, 1830
GsaI	CCCAGC	1 cut(s)	1171
GsuI	CTGGAG	2 cut(s)	927, 1488
HaeII	RGCGCY	1 cut(s)	2099
HaeIII	GGCC	8 cut(s)	36, 315, 835, 1299, 1480, 1730, 1868, 1886
HapII	CCGG	4 cut(s)	12, 25, 229, 1887
HgaI	GACGC	1 cut(s)	677
HhaI	GCGC	1 cut(s)	2098
Hin1I	GRCGYC	1 cut(s)	2096
Hin6I	GCGC	1 cut(s)	2096
HinP1I	GCGC	1 cut(s)	2096
HincII	GTYRAC	1 cut(s)	1190
HindII	GTYRAC	1 cut(s)	1190
HindIII	AAGCTT	2 cut(s)	1373, 1908
HinfI	GANTC	4 cut(s)	148, 1175, 1417, 1943
HpaII	CCGG	4 cut(s)	12, 25, 229, 1887
HphI	GGTGA	5 cut(s)	395, 1229, 1262, 1346, 1583
Hpy166II	GTNNAC	2 cut(s)	1190, 1384
Hpy188III	TCNNGA	9 cut(s)	152, 365, 440, 527, 1259, 1269, 1976, 2014, 2181
Hpy8I	GTNNAC	2 cut(s)	1190, 1384
Hpy99I	CGWCG	1 cut(s)	693
HpyAV	CCTTC	6 cut(s)	90, 229, 860, 1325, 1491, 2016
HpyCH4III	ACNGT	5 cut(s)	583, 661, 1585, 1817, 1994
HpyCH4IV	ACGT	1 cut(s)	1522
HpyF10VI	GCNNNNNNNGC	7 cut(s)	67, 124, 187, 790, 1055, 1139, 1835
HpyF3I	CTNAG	5 cut(s)	273, 535, 1870, 1977, 2065
HpySE526I	ACGT	1 cut(s)	1522
Hsp92I	GRCGYC	1 cut(s)	2096
HspAI	GCGC	1 cut(s)	2096
KasI	GGCGCC	1 cut(s)	2095
Ksp22I	TGATCA	2 cut(s)	96, 193
Kzo9I	GATC	5 cut(s)	96, 193, 276, 490, 892
LmnI	GCTCC	2 cut(s)	6, 2030
Lsp1109I	GCAGC	5 cut(s)	52, 1145, 1215, 1557, 1816
LweI	GCATC	8 cut(s)	121, 268, 387, 433, 541, 656, 918, 1825
MaeI	CTAG	8 cut(s)	302, 794, 875, 909, 966, 1668, 2022, 2046
MaeII	ACGT	1 cut(s)	1522
MaeIII	GTNAC	5 cut(s)	418, 426, 607, 1235, 1994
MalI	GATC	5 cut(s)	98, 195, 278, 492, 894
MboI	GATC	5 cut(s)	96, 193, 276, 490, 892
MfeI	CAATTG	1 cut(s)	486
MflI	RGATCY	2 cut(s)	276, 892
MhlI	GDGCHC	3 cut(s)	11, 183, 1935
MlsI	TGGCCA	2 cut(s)	36, 835
MluNI	TGGCCA	2 cut(s)	36, 835
Mly113I	GGCGCC	1 cut(s)	2096
MlyI	GAGTC	2 cut(s)	157, 1184
MmeI	TCCRAC	1 cut(s)	325
Mox20I	TGGCCA	2 cut(s)	36, 835
Mph1103I	ATGCAT	2 cut(s)	671, 781
MroXI	GAANNNNTTC	2 cut(s)	1522, 2188
MscI	TGGCCA	2 cut(s)	36, 835
MseI	TTAA	7 cut(s)	17, 30, 216, 474, 1349, 1397, 1680
MslI	CAYNNNNRTG	1 cut(s)	1248
Msp20I	TGGCCA	2 cut(s)	36, 835
MspI	CCGG	4 cut(s)	12, 25, 229, 1887
MspR9I	CCNGG	6 cut(s)	12, 230, 318, 1248, 1467, 1644
MunI	CAATTG	1 cut(s)	486
Mva1269I	GAATGC	2 cut(s)	639, 781
MvaI	CCWGG	4 cut(s)	318, 1248, 1467, 1644
MvnI	CGCG	1 cut(s)	693
MwoI	GCNNNNNNNGC	7 cut(s)	67, 124, 187, 790, 1055, 1139, 1835
NarI	GGCGCC	1 cut(s)	2096
NciI	CCSGG	2 cut(s)	12, 230
NdeI	CATATG	2 cut(s)	758, 1894
NdeII	GATC	5 cut(s)	96, 193, 276, 490, 892
NheI	GCTAGC	1 cut(s)	1667
NlaIV	GGNNCC	5 cut(s)	8, 349, 894, 2097, 2113
NmeAIII	GCCGAG	1 cut(s)	162
NmuCI	GTSAC	2 cut(s)	1235, 1994
NsiI	ATGCAT	2 cut(s)	671, 781
OliI	CACNNNNGTG	1 cut(s)	1248
PceI	AGGCCT	1 cut(s)	1480
PcsI	WCGNNNNNNNCGW	1 cut(s)	1998
PctI	GAATGC	2 cut(s)	639, 781
PdmI	GAANNNNTTC	2 cut(s)	1522, 2188
PfeI	GAWTC	2 cut(s)	1417, 1943
PfoI	TCCNGGA	1 cut(s)	1465
PkrI	GCNGC	7 cut(s)	42, 1135, 1230, 1547, 1702, 1732, 1831
PleI	GAGTC	2 cut(s)	156, 1183
PluTI	GGCGCC	1 cut(s)	2099
PpsI	GAGTC	2 cut(s)	156, 1183
PshBI	ATTAAT	1 cut(s)	30
Psp124BI	GAGCTC	1 cut(s)	1935
Psp1406I	AACGTT	1 cut(s)	1522
Psp6I	CCWGG	4 cut(s)	316, 1246, 1465, 1642
PspFI	CCCAGC	1 cut(s)	1167
PspGI	CCWGG	4 cut(s)	316, 1246, 1465, 1642
PspN4I	GGNNCC	5 cut(s)	8, 349, 894, 2097, 2113
PspPI	GGNCC	6 cut(s)	77, 314, 347, 613, 1297, 1889
PsrI	GAACNNNNNNTAC	2 cut(s)	953, 985
PstI	CTGCAG	1 cut(s)	1233
PsuI	RGATCY	2 cut(s)	276, 892
RsaI	GTAC	2 cut(s)	663, 988
RsaNI	GTAC	2 cut(s)	662, 987
RseI	CAYNNNNRTG	1 cut(s)	1248
SacI	GAGCTC	1 cut(s)	1935
SaqAI	TTAA	7 cut(s)	17, 30, 216, 474, 1349, 1397, 1680
SatI	GCNGC	7 cut(s)	41, 1134, 1229, 1546, 1701, 1731, 1830
Sau3AI	GATC	5 cut(s)	96, 193, 276, 490, 892
Sau96I	GGNCC	6 cut(s)	77, 314, 347, 613, 1297, 1889
ScaI	AGTACT	1 cut(s)	988
SchI	GAGTC	2 cut(s)	157, 1184
ScrFI	CCNGG	6 cut(s)	12, 230, 318, 1248, 1467, 1644
SduI	GDGCHC	3 cut(s)	11, 183, 1935
SfaNI	GCATC	8 cut(s)	121, 268, 387, 433, 541, 656, 918, 1825
SfcI	CTRYAG	2 cut(s)	601, 1229
SfoI	GGCGCC	1 cut(s)	2097
SinI	GGWCC	4 cut(s)	77, 347, 613, 1889
SmiI	ATTTAAAT	1 cut(s)	1350
SmiMI	CAYNNNNRTG	1 cut(s)	1248
SmlI	CTYRAG	3 cut(s)	113, 982, 2075
SmoI	CTYRAG	3 cut(s)	113, 982, 2075
SpeI	ACTAGT	1 cut(s)	2045
SseBI	AGGCCT	1 cut(s)	1480
SsiI	CCGC	7 cut(s)	134, 569, 652, 693, 713, 1701, 1731
SspDI	GGCGCC	1 cut(s)	2095
SspMI	CTAG	8 cut(s)	302, 794, 875, 909, 966, 1668, 2022, 2046
SstI	GAGCTC	1 cut(s)	1935
StuI	AGGCCT	1 cut(s)	1480
StyD4I	CCNGG	6 cut(s)	10, 228, 316, 1246, 1465, 1642
StyI	CCWWGG	1 cut(s)	908
SwaI	ATTTAAAT	1 cut(s)	1350
TaaI	ACNGT	5 cut(s)	583, 661, 1585, 1817, 1994
TaiI	ACGT	1 cut(s)	1525
TaqI	TCGA	4 cut(s)	364, 1935, 2001, 2013
TatI	WGTACW	1 cut(s)	986
TauI	GCSGC	2 cut(s)	1703, 1733
TfiI	GAWTC	2 cut(s)	1417, 1943
Tru1I	TTAA	7 cut(s)	17, 30, 216, 474, 1349, 1397, 1680
Tru9I	TTAA	7 cut(s)	17, 30, 216, 474, 1349, 1397, 1680
TscAI	CASTG	3 cut(s)	1135, 1238, 1812
TseFI	GTSAC	2 cut(s)	1235, 1994
TseI	GCWGC	5 cut(s)	40, 1133, 1228, 1545, 1829
Tsp45I	GTSAC	2 cut(s)	1235, 1994
TspGWI	ACGGA	4 cut(s)	437, 492, 626, 1175
TspRI	CASTG	3 cut(s)	1135, 1238, 1812
VpaK11BI	GGWCC	4 cut(s)	77, 347, 613, 1889
VspI	ATTAAT	1 cut(s)	30
XapI	RAATTY	6 cut(s)	618, 646, 1029, 1453, 1843, 2155
XmaJI	CCTAGG	1 cut(s)	908
XmiI	GTMKAC	1 cut(s)	1383
XmnI	GAANNNNTTC	2 cut(s)	1522, 2188
XspI	CTAG	8 cut(s)	302, 794, 875, 909, 966, 1668, 2022, 2046
ZrmI	AGTACT	1 cut(s)	988
Zsp2I	ATGCAT	2 cut(s)	671, 781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.