Rorug06G0422700

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005853
Physical Location & Seq
Forward (+)
56100312 .. 56106298
5987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0422700.1

Sequence Viewer

Length: 2463 bp
ATGGTGACGGAGGATTGTTGGCCAACAATGGATCTACTGCGGTCGGAGCCAATGCAACTGGTGCACCTCATCATTCCGATCGAATCGTCTCGCCGGGCCATCTCCTACCTCGGCGACCTTGGCCTTTTTCAATTCAAAGATCTCAATGCGGAGAAGAGTCCGTTCCAGAGAACATATGCCGCTCAGATCAAACGATGTGGGGAAATGGCTCGAAGGTTACGTTTCTTCAGAGATCAGATGAAGAAGGCTGGTTTGTCACATTCAACAATGTCTACAATGAGCGATGATAATGATCTTGATCGCTTGGAGGTTAAACTCGGTGAACTTGAAGCTGATCTATTAGAAATGAATTCAAACAATGAACAATTACAACGCACTTATAGTGAGCTATTAGAATACAAGCTTGTACTGCAGAAGGCTGGCGGGTTTTTTAGTTCAGCTCAAAGTAGTGCCACAGCTCAGCAAAGAGAAATTGAAGTGCAGCCCCTGGGTGAAAGATCCATTGACAGTCCATTATTACTCGAACAAGAAATGACAACAGATCCATCGAAGCATGTAAAGCTTGGGTCCGTGAGTGGTCTTGTTCCAAGAGAAAAGTCTTTGGCATTTGAAAGGATTTTATTTCGTGCCACCAGGGGTAATGTATTTCTGAAGCAAGCTGTGGTCAATGGCCCTGTTGTTGATCCTGTGTCAGGAGAGAAGGTTGAGAAAAACGTATTCATCATCTTCTATTCTGGCGAAAGGGCAAAGAATAAAATTCTAAAAATATGTGAGGCTTTTAGAGCAAACCGTTACCCATTTACAGATGACTTGGGAAAACAATTTCAGATGATTACAGAGGTGTCTGGAAAACTTTCAGAGCTGAAGTCTACCATAGATGCTGGACTATTGCACCGGAACAGTTTATTACAGACAATTGGCCATCAATATGAGCAATGGAACCTTCTGGTAAAGAAGGAAAAATCCGTCTACCACACCTTAAACATGCTCAGCATTGATGTGACAAAGATGTGTCTTGTTGCAGAAGGTTGGTGTCCTGTATCCGCATCAATTCAGATCCAAAACGCATTGCAGCAGGCAACCTTTGATACCAACTGTCAAGTTGGGGCCATATTCCAGGTTTTGCAGACAAAGGAATCGCCACCAACATATTTCCGTACAAACAAATTTACTTCTGCTTTTCAAGAAATTGTTGACGCATATGGGGTTGCCAAGTATCGGGAGGCTAATCCTGGTGTATACACCATTGTCACTTTCCCTTTCCTTTTCGCTGTGATGTTTGGAGATTGGGGGCATGGTATTTGCTTGTTGCTGGCAACACTATATTTCATCATCAGGGAGAGGAAGTTTTCTAGTCAGAAACTTGGGGACATTATTGAAATGACGTTTGGTGGCCGTTATGTTATTTTTATGATGGCGCTGTTCTCAATTTACACAGGCTTGATATATAATGAATTCTTTTCAGTCCCATTTGAAATATTTGGGCCTTCTGCCTATGGGTGCCGTGACCCATCCTGCAGGGATGCCACTACAGCAGGTTTGACTAAGGTGCGTGACACCTATCCATTTGGTGTGGATCCTAAATGGCACGGTTCCAGGTCTGAATTACCCTTTCTTAACTCGTTGAAAATGAAGATGTCAATCCTACTTGGAGTAGCCCAGATGAATCTTGGAATTGTATTAAGTTACTTCAATGCAAAATATTTTGCGGATAAGCTGAATATCTGGTACCAATTTGTTCCTCAGATGATATTTCTAAACAGTCTGTTTGGCTACCTTTCACTCCTCATTATTGTGAAATGGTGCACTGGTTCAAAAGCTGATTTGTATCATGTAATGATATACATGTTCCTGAGTCCTTTCGAAGATCTGGGTGAAAATCAGCTCTTTTGGGGGCAAGGATTCCTTCAGGTTCTATTATTACTATCCGCTCTTGTTGCTGTGCCATGGATGCTGTTTCCAAAGCCTTATTTCTTGAAAAAGCAGCATGAGGAGAGGCACCAAGGTCAATCATACGCGTTGCTTCTGACTGGGGAGGACCCTCTTGAAGATGACCAGGAAGACAATCACAATTCAAAAGGTCATGAGGAATTTGAATTCAGCGAGATCTTTGTACACCAACTCATACATACCATAGAGTTTGTGCTTGGAGCAGTGTCCAATACAGCTTCATATCTACGTTTATGGGCACTCAGCTTAGCACATTCAGAGTTGTCCAGTGTATTTTACGACAAGGTTCTGCTTCTAGCATGGGGGTTCAACAATGTCATTATTCTAATTATTGGCATAATCGTTTTTATAAGTGCAACTGTTGGAGTGCTACTAGTGATGGAGACCTTAAGTGCATTCTTGCATGCCTTGAGGCTTCACTGGGTGGAGTTTCAGAACAAGTTTTACGAGGGCACTGGTTACAAATTCTGCCCTTTTTCATTTGCATTACTCAGTAGTGAGGATGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

820

Amino Acids

93.42

Weight (kDa)

5.91

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 36 - 811 1.6e-303 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2300
Acc36I ACCTGC 1 cut(s) 1526
Acc65I GGTACC 1 cut(s) 1728
AccB1I GGYRCC 3 cut(s) 1500, 1728, 1998
AccBSI CCGCTC 2 cut(s) 182, 1931
AccI GTMKAC 4 cut(s) 272, 869, 969, 1239
AccII CGCG 1 cut(s) 2018
AciI CCGC 7 cut(s) 40, 149, 180, 423, 1044, 1709, 1929
AclWI GGATC 7 cut(s) 39, 492, 536, 677, 1051, 1571, 1584
AcoI YGGCCR 3 cut(s) 20, 919, 1393
AcsI RAATTY 7 cut(s) 349, 756, 1166, 1454, 2090, 2096, 2414
AcuI CTGAAG 4 cut(s) 211, 671, 884, 1892
AdeI CACNNNGTG 1 cut(s) 2374
AfaI GTAC 4 cut(s) 408, 1159, 1730, 2115
AfiI CCNNNNNNNGG 2 cut(s) 692, 1218
AflII CTTAAG 1 cut(s) 2338
AflIII ACRYGT 2 cut(s) 1845, 2016
AhlI ACTAGT 1 cut(s) 2323
AjnI CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
AjuI GAANNNNNNNTTGG 2 cut(s) 1371, 1403
Alw21I GWGCWC 2 cut(s) 66, 1808
Alw26I GTCTC 2 cut(s) 93, 2327
Alw44I GTGCAC 2 cut(s) 62, 1804
AlwI GGATC 7 cut(s) 39, 492, 536, 677, 1051, 1571, 1584
AlwNI CAGNNNCTG 1 cut(s) 487
AoxI GGCC 8 cut(s) 20, 96, 121, 670, 919, 1107, 1393, 1484
ApaLI GTGCAC 2 cut(s) 62, 1804
ApeKI GCWGC 3 cut(s) 481, 1072, 1984
ApoI RAATTY 7 cut(s) 349, 756, 1166, 1454, 2090, 2096, 2414
Asp700I GAANNNNTTC 1 cut(s) 853
Asp718I GGTACC 1 cut(s) 1728
AspLEI GCGC 1 cut(s) 1420
AspS9I GGNCC 6 cut(s) 96, 567, 671, 1107, 1484, 2038
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 4 cut(s) 16, 332, 503, 1886
AsuII TTCGAA 1 cut(s) 1863
AvaII GGWCC 2 cut(s) 567, 2038
BaeGI GKGCMC 4 cut(s) 66, 1808, 2191, 2405
BalI TGGCCA 2 cut(s) 22, 921
BamHI GGATCC 1 cut(s) 1576
BanI GGYRCC 3 cut(s) 1500, 1728, 1998
BbsI GAAGAC 1 cut(s) 2067
Bbv12I GWGCWC 2 cut(s) 66, 1808
BbvI GCAGC 3 cut(s) 493, 1084, 1996
BccI CCATC 6 cut(s) 107, 553, 930, 1408, 1519, 2323
BceAI ACGGC 2 cut(s) 1380, 1488
BciT130I CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
BciVI GTATCC 1 cut(s) 1051
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 93, 2327
BcuI ACTAGT 1 cut(s) 2323
BfaI CTAG 3 cut(s) 1353, 2246, 2324
BfmI CTRYAG 3 cut(s) 410, 1516, 1530
BfoI RGCGCY 1 cut(s) 1421
BfrI CTTAAG 1 cut(s) 2338
BfuAI ACCTGC 1 cut(s) 1526
BfuI GTATCC 1 cut(s) 1051
BglII AGATCT 3 cut(s) 139, 1867, 2106
BisI GCNGC 4 cut(s) 180, 482, 1073, 1985
BlpI GCTNAGC 3 cut(s) 459, 989, 2197
BlsI GCNGC 4 cut(s) 181, 483, 1074, 1986
Bme1390I CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
Bme18I GGWCC 2 cut(s) 567, 2038
BmgT120I GGNCC 6 cut(s) 96, 567, 671, 1107, 1484, 2038
BmrFI CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
BmrI ACTGGG 2 cut(s) 2040, 2380
BmsI GCATC 4 cut(s) 868, 1055, 1513, 1941
BmuI ACTGGG 2 cut(s) 2040, 2380
BpiI GAAGAC 1 cut(s) 2067
Bpu1102I GCTNAGC 3 cut(s) 459, 989, 2197
Bpu14I TTCGAA 1 cut(s) 1863
BpuEI CTTGAG 1 cut(s) 2380
BpuMI CCSGG 1 cut(s) 95
BsaBI GATNNNNATC 4 cut(s) 291, 297, 1640, 1827
BsaI GGTCTC 1 cut(s) 2327
BsaJI CCNNGG 7 cut(s) 109, 118, 486, 487, 633, 1946, 2002
BsaWI WCCGGW 1 cut(s) 894
BsaXI ACNNNNNCTCC 4 cut(s) 687, 717, 1331, 1361
Bsc4I CCNNNNNNNGG 2 cut(s) 692, 1218
Bse1I ACTGG 6 cut(s) 63, 1813, 2035, 2217, 2375, 2410
Bse3DI GCAATG 2 cut(s) 941, 1067
Bse8I GATNNNNATC 4 cut(s) 291, 297, 1640, 1827
BseBI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
BseDI CCNNGG 7 cut(s) 109, 118, 486, 487, 633, 1946, 2002
BseGI GGATG 4 cut(s) 1511, 1528, 1956, 2458
BseJI GATNNNNATC 4 cut(s) 291, 297, 1640, 1827
BseLI CCNNNNNNNGG 2 cut(s) 692, 1218
BseMI GCAATG 2 cut(s) 941, 1067
BseMII CTCAG 7 cut(s) 197, 473, 1003, 1757, 1844, 2206, 2455
BseNI ACTGG 6 cut(s) 63, 1813, 2035, 2217, 2375, 2410
BseRI GAGGAG 2 cut(s) 1775, 2006
BseSI GKGCMC 4 cut(s) 66, 1808, 2191, 2405
BseXI GCAGC 3 cut(s) 493, 1084, 1996
BsgI GTGCAG 1 cut(s) 500
Bsh1236I CGCG 1 cut(s) 2018
Bsh1285I CGRYCG 2 cut(s) 44, 81
BshFI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
BshNI GGYRCC 3 cut(s) 1500, 1728, 1998
BsiEI CGRYCG 2 cut(s) 44, 81
BsiHKAI GWGCWC 2 cut(s) 66, 1808
BsiSI CCGG 2 cut(s) 94, 895
BslFI GGGAC 2 cut(s) 1382, 1451
BslI CCNNNNNNNGG 2 cut(s) 692, 1218
BsmAI GTCTC 2 cut(s) 93, 2327
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 1382, 1451
BsmI GAATGC 1 cut(s) 2345
BsnI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
Bso31I GGTCTC 1 cut(s) 2327
Bsp119I TTCGAA 1 cut(s) 1863
Bsp1286I GDGCHC 4 cut(s) 66, 1808, 2191, 2405
Bsp1407I TGTACA 1 cut(s) 2113
Bsp1720I GCTNAGC 3 cut(s) 459, 989, 2197
Bsp19I CCATGG 1 cut(s) 1946
BspACI CCGC 7 cut(s) 40, 149, 180, 423, 1044, 1709, 1929
BspANI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
BspCNI CTCAG 7 cut(s) 196, 472, 1002, 1756, 1845, 2205, 2454
BspFNI CGCG 1 cut(s) 2018
BspHI TCATGA 1 cut(s) 2083
BspMAI CTGCAG 2 cut(s) 414, 1520
BspMI ACCTGC 1 cut(s) 1526
BspPI GGATC 7 cut(s) 39, 492, 536, 677, 1051, 1571, 1584
BspT104I TTCGAA 1 cut(s) 1863
BspT107I GGYRCC 3 cut(s) 1500, 1728, 1998
BspTI CTTAAG 1 cut(s) 2338
BspTNI GGTCTC 1 cut(s) 2327
BsrBI CCGCTC 2 cut(s) 182, 1931
BsrDI GCAATG 2 cut(s) 941, 1067
BsrGI TGTACA 1 cut(s) 2113
BsrI ACTGG 6 cut(s) 63, 1813, 2035, 2217, 2375, 2410
BssECI CCNNGG 7 cut(s) 109, 118, 486, 487, 633, 1946, 2002
BssNAI GTATAC 1 cut(s) 1240
BssT1I CCWWGG 3 cut(s) 118, 1946, 2002
Bst1107I GTATAC 1 cut(s) 1240
Bst2UI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
Bst4CI ACNGT 7 cut(s) 509, 791, 902, 1097, 1592, 1763, 2311
Bst6I CTCTTC 1 cut(s) 149
BstAFI CTTAAG 1 cut(s) 2338
BstAPI GCANNNNNTGC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 2113
BstBI TTCGAA 1 cut(s) 1863
BstC8I GCNNGC 5 cut(s) 421, 657, 1077, 1314, 2355
BstDEI CTNAG 9 cut(s) 183, 459, 989, 1545, 1743, 1853, 2192, 2197, 2441
BstDSI CCRYGG 1 cut(s) 1946
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 4 cut(s) 1511, 1528, 1956, 2458
BstFNI CGCG 1 cut(s) 2018
BstH2I RGCGCY 1 cut(s) 1421
BstHHI GCGC 1 cut(s) 1420
BstMAI GTCTC 2 cut(s) 93, 2327
BstMCI CGRYCG 2 cut(s) 44, 81
BstMWI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 409, 559, 782, 1532, 1937, 1951
BstNI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
BstNSI RCATGY 4 cut(s) 557, 988, 1849, 2357
BstSCI CCNGG 7 cut(s) 93, 486, 632, 1116, 1231, 1595, 2055
BstSFI CTRYAG 3 cut(s) 410, 1516, 1530
BstSLI GKGCMC 4 cut(s) 66, 1808, 2191, 2405
BstUI CGCG 1 cut(s) 2018
BstV1I GCAGC 3 cut(s) 493, 1084, 1996
BstV2I GAAGAC 1 cut(s) 2067
BstX2I RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
BstYI RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
BstZ17I GTATAC 1 cut(s) 1240
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
BtgI CCRYGG 1 cut(s) 1946
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 4 cut(s) 1511, 1528, 1956, 2458
BtsI GCAGTG 1 cut(s) 2160
BtsIMutI CAGTG 5 cut(s) 1806, 2160, 2224, 2368, 2403
BveI ACCTGC 1 cut(s) 1526
Cac8I GCNNGC 5 cut(s) 421, 657, 1077, 1314, 2355
CaiI CAGNNNCTG 1 cut(s) 487
CciI TCATGA 1 cut(s) 2083
CfoI GCGC 1 cut(s) 1420
Cfr13I GGNCC 6 cut(s) 96, 567, 671, 1107, 1484, 2038
CseI GACGC 1 cut(s) 1205
Csp6I GTAC 4 cut(s) 407, 1158, 1729, 2114
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviQI GTAC 4 cut(s) 407, 1158, 1729, 2114
DdeI CTNAG 9 cut(s) 183, 459, 989, 1545, 1743, 1853, 2192, 2197, 2441
DraIII CACNNNGTG 1 cut(s) 2374
EaeI YGGCCR 3 cut(s) 20, 919, 1393
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 3 cut(s) 118, 1946, 2002
Eco31I GGTCTC 1 cut(s) 2327
Eco47I GGWCC 2 cut(s) 567, 2038
Eco57I CTGAAG 4 cut(s) 211, 671, 884, 1892
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 2038
EcoRI GAATTC 3 cut(s) 349, 1454, 2096
EcoRII CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
EcoT14I CCWWGG 3 cut(s) 118, 1946, 2002
ErhI CCWWGG 3 cut(s) 118, 1946, 2002
Esp3I CGTCTC 1 cut(s) 93
FalI AAGNNNNNCTT 2 cut(s) 1890, 1922
FaqI GGGAC 2 cut(s) 1382, 1451
FauI CCCGC 1 cut(s) 416
FauNDI CATATG 2 cut(s) 175, 1201
FblI GTMKAC 4 cut(s) 272, 869, 969, 1239
Fnu4HI GCNGC 4 cut(s) 180, 482, 1073, 1985
FokI GGATG 3 cut(s) 1498, 1535, 1963
Fsp4HI GCNGC 4 cut(s) 180, 482, 1073, 1985
FspBI CTAG 3 cut(s) 1353, 2246, 2324
GlaI GCGC 1 cut(s) 1419
GluI GCNGC 4 cut(s) 180, 482, 1073, 1985
HaeII RGCGCY 1 cut(s) 1421
HaeIII GGCC 8 cut(s) 22, 98, 123, 672, 921, 1109, 1395, 1486
HapII CCGG 2 cut(s) 94, 895
HgaI GACGC 1 cut(s) 1205
HhaI GCGC 1 cut(s) 1420
Hin6I GCGC 1 cut(s) 1418
HinP1I GCGC 1 cut(s) 1418
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 2 cut(s) 401, 560
HinfI GANTC 6 cut(s) 83, 157, 1136, 1666, 1855, 1902
HpaII CCGG 2 cut(s) 94, 895
HphI GGTGA 4 cut(s) 16, 332, 503, 1886
Hpy166II GTNNAC 9 cut(s) 64, 273, 323, 870, 970, 1195, 1240, 1806, 2116
Hpy8I GTNNAC 9 cut(s) 64, 273, 323, 870, 970, 1195, 1240, 1806, 2116
HpyAV CCTTC 9 cut(s) 207, 238, 409, 694, 949, 953, 1019, 1497, 1916
HpyCH4III ACNGT 7 cut(s) 509, 791, 902, 1097, 1592, 1763, 2311
HpyCH4IV ACGT 4 cut(s) 220, 714, 1385, 2179
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 409, 559, 782, 1532, 1937, 1951
HpyF3I CTNAG 9 cut(s) 183, 459, 989, 1545, 1743, 1853, 2192, 2197, 2441
HpySE526I ACGT 4 cut(s) 220, 714, 1385, 2179
HspAI GCGC 1 cut(s) 1418
KpnI GGTACC 1 cut(s) 1732
LmnI GCTCC 2 cut(s) 46, 2150
Lsp1109I GCAGC 3 cut(s) 493, 1084, 1996
LweI GCATC 4 cut(s) 868, 1055, 1513, 1941
MaeI CTAG 3 cut(s) 1353, 2246, 2324
MaeII ACGT 4 cut(s) 220, 714, 1385, 2179
MbiI CCGCTC 2 cut(s) 182, 1931
MboII GAAGA 8 cut(s) 166, 217, 253, 718, 1645, 1877, 2060, 2072
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
MhlI GDGCHC 4 cut(s) 66, 1808, 2191, 2405
MlsI TGGCCA 2 cut(s) 22, 921
MluI ACGCGT 1 cut(s) 2016
MluNI TGGCCA 2 cut(s) 22, 921
MlyI GAGTC 2 cut(s) 166, 1864
MmeI TCCRAC 2 cut(s) 24, 2293
Mox20I TGGCCA 2 cut(s) 22, 921
MroXI GAANNNNTTC 1 cut(s) 853
MscI TGGCCA 2 cut(s) 22, 921
MseI TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
MslI CAYNNNNRTG 3 cut(s) 927, 998, 1793
Msp20I TGGCCA 2 cut(s) 22, 921
MspCI CTTAAG 1 cut(s) 2338
MspI CCGG 2 cut(s) 94, 895
MspR9I CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 2345
MvaI CCWGG 6 cut(s) 488, 634, 1118, 1233, 1597, 2057
MvnI CGCG 1 cut(s) 2018
MwoI GCNNNNNNNGC 9 cut(s) 46, 61, 120, 409, 559, 782, 1532, 1937, 1951
NciI CCSGG 1 cut(s) 95
NcoI CCATGG 1 cut(s) 1946
NdeI CATATG 2 cut(s) 175, 1201
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 6 cut(s) 4, 255, 1000, 1249, 1505, 1553
NspI RCATGY 4 cut(s) 557, 988, 1849, 2357
NspV TTCGAA 1 cut(s) 1863
PaeI GCATGC 1 cut(s) 2357
PagI TCATGA 1 cut(s) 2083
PasI CCCWGGG 1 cut(s) 487
PciI ACATGT 1 cut(s) 1845
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PctI GAATGC 1 cut(s) 2345
PdmI GAANNNNTTC 1 cut(s) 853
PfeI GAWTC 4 cut(s) 83, 1136, 1666, 1902
PkrI GCNGC 4 cut(s) 181, 483, 1074, 1986
Ple19I CGATCG 1 cut(s) 81
PleI GAGTC 2 cut(s) 165, 1863
PpsI GAGTC 2 cut(s) 165, 1863
PpuMI RGGWCCY 1 cut(s) 2038
PscI ACATGT 1 cut(s) 1845
PsiI TTATAA 1 cut(s) 2300
Psp5II RGGWCCY 1 cut(s) 2038
Psp6I CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
PspGI CCWGG 6 cut(s) 486, 632, 1116, 1231, 1595, 2055
PspPI GGNCC 6 cut(s) 96, 567, 671, 1107, 1484, 2038
PspPPI RGGWCCY 1 cut(s) 2038
PsrI GAACNNNNNNTAC 2 cut(s) 2378, 2410
PstI CTGCAG 2 cut(s) 414, 1520
PstNI CAGNNNCTG 1 cut(s) 487
PsuI RGATCY 8 cut(s) 31, 139, 497, 541, 1056, 1576, 1867, 2106
PvuI CGATCG 1 cut(s) 81
RsaI GTAC 4 cut(s) 408, 1159, 1730, 2115
RsaNI GTAC 4 cut(s) 407, 1158, 1729, 2114
RseI CAYNNNNRTG 3 cut(s) 927, 998, 1793
SaqAI TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
SatI GCNGC 4 cut(s) 180, 482, 1073, 1985
Sau96I GGNCC 6 cut(s) 96, 567, 671, 1107, 1484, 2038
SbfI CCTGCAGG 1 cut(s) 1520
SchI GAGTC 2 cut(s) 166, 1864
ScrFI CCNGG 7 cut(s) 95, 488, 634, 1118, 1233, 1597, 2057
SdaI CCTGCAGG 1 cut(s) 1520
SduI GDGCHC 4 cut(s) 66, 1808, 2191, 2405
SfaNI GCATC 4 cut(s) 868, 1055, 1513, 1941
SfcI CTRYAG 3 cut(s) 410, 1516, 1530
SfuI TTCGAA 1 cut(s) 1863
SinI GGWCC 2 cut(s) 567, 2038
SmiMI CAYNNNNRTG 3 cut(s) 927, 998, 1793
SmlI CTYRAG 2 cut(s) 2338, 2359
SmoI CTYRAG 2 cut(s) 2338, 2359
SpeI ACTAGT 1 cut(s) 2323
SphI GCATGC 1 cut(s) 2357
Sse8387I CCTGCAGG 1 cut(s) 1520
SsiI CCGC 7 cut(s) 40, 149, 180, 423, 1044, 1709, 1929
SspI AATATT 2 cut(s) 1479, 1703
SspMI CTAG 3 cut(s) 1353, 2246, 2324
StyD4I CCNGG 7 cut(s) 93, 486, 632, 1116, 1231, 1595, 2055
StyI CCWWGG 3 cut(s) 118, 1946, 2002
TaaI ACNGT 7 cut(s) 509, 791, 902, 1097, 1592, 1763, 2311
TaiI ACGT 4 cut(s) 223, 717, 1388, 2182
TaqI TCGA 5 cut(s) 81, 211, 522, 548, 1863
TatI WGTACW 2 cut(s) 406, 2113
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 4 cut(s) 83, 1136, 1666, 1902
Tru1I TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
Tru9I TTAA 5 cut(s) 312, 980, 1617, 1682, 2339
TscAI CASTG 5 cut(s) 1813, 2160, 2224, 2375, 2410
TseFI GTSAC 6 cut(s) 4, 255, 1000, 1249, 1505, 1553
TseI GCWGC 3 cut(s) 481, 1072, 1984
Tsp45I GTSAC 6 cut(s) 4, 255, 1000, 1249, 1505, 1553
TspGWI ACGGA 5 cut(s) 23, 150, 559, 955, 1145
TspRI CASTG 5 cut(s) 1813, 2160, 2224, 2375, 2410
Vha464I CTTAAG 1 cut(s) 2338
VneI GTGCAC 2 cut(s) 62, 1804
VpaK11BI GGWCC 2 cut(s) 567, 2038
XagI CCTNNNNNAGG 1 cut(s) 690
XapI RAATTY 7 cut(s) 349, 756, 1166, 1454, 2090, 2096, 2414
XceI RCATGY 4 cut(s) 557, 988, 1849, 2357
XcmI CCANNNNNNNNNTGG 1 cut(s) 1667
XmiI GTMKAC 4 cut(s) 272, 869, 969, 1239
XmnI GAANNNNTTC 1 cut(s) 853
XspI CTAG 3 cut(s) 1353, 2246, 2324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.