Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0005853

Physical Location & Seq

Genomic Coordinates Reverse (-)

67147834 .. 67152581

Gene Structure 4748 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005369

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2214 bp

ATGGGGAGCCCCGGGTTAAGCAACCGGAATAATGGCCAGCAGCGATTAGGCATTACTGAGCCGATTTCGTTGGGCGGTCCGTCGGAGGAGGATGTGATCAAAAGCCGTGAGCTTGAGAAGTTTTTGCATGACGCTGGATTGTATGAGAGTCAGGAAGAGGCTGTAAGTAGGGAAGAGGTGCTTGGCAGGCTTGATCAGATTGTGAAGATATGGGTTAAGACCATTAGCCGGGCGAAGGGTTTGAACGATGAACTGGTGCATCAAGCAAATGCTAAGATCTTCACGTTTGGTTCGTATAGGCTAGGGGTTCATGGCCCTGGTGCAGATATAGACACACTCTGTGTTGGACCCAGACATGCAACTCGAGAAGAAGATTTCTTTGGTGAGTTACACAGGATGCTATCTGAAATGTCTGAAGTAACGGAGTTGCATCCTGTTCCTGATGCCCATGTTCCCGTTATGAGGTTCAAGTTTAACGGAGTATCAATTGATCTTCTTTATGCAAAACTATCTCTTTATATTATTCCTGAGGACTTAGATGTATCACAAGATGCAATACTACATAGTGCAGATGAACAGACCGTTCGTAGTCTCAATGGCTGTAGAGTTACAGACCAAATTCTGCGTCTAGTCCCAAGCATTCAGAATTTCCGCACAACACTAAGATGCATGAGGTTATGGGCAAAGCGTCGTGGTATTTATTCAAATGTTTCAGGATTTCTTGGTGGTATAAACTGGGCATTGCTTGTTGCTCGCATATGCCAACTTTACCCGAATGCATTGCCTAATATGCTAGTATCTCGGTTCTTCAGGGTCTATACTCAGTGGCGTTGGCCAAATCCAGTAATGCTCTGTGCTATCGAAGAAGGGTCTCTAGGACTTCCGGTTTGGGATCCCAGAAGAAATCCAAGGGACAGATATCATTTGATGCCTATAATTACTCCAGCTTATCCTTGCATGAACTCTAGCTACAATGTGTCCTCAAGTACTCTGCGTATCATGTTAGAGGAGTTTCAGAGGGGAAATGAAATTTGTGAGGCTATGGAAGCAAATAAGGCTGATTGGGATACCCTTTTTGAGTCGTATGCTTTTTTTGAAGCATATAAGAATTATTTACAGATAGACATCAGTGCAGCAAATGCTGATGGCTTTAGAAAATGGAAAGGTTGGGTTGAGTCCCGTCTCCGTCAACTAACATTGAAGATTGAGAGGAAAACTTATGAAATGCTGCAGTGTCACCCACACCCAGGTGATTTTCCAGATAAATCCAGACCTTTTCATAGCTCATACTTCATGGGCCTACAACGAAAACAAGGAGTTCCAGTCAAGGAAGGTGAGCACTTTGATTTAAGTAAAGCTGTTGATGACTTCAAGCTTGCTGTCTACCAGTATACATTAAGGGAAATTGGAATGGAGATCCGTGTATCCCATGTAAAACGCAGGGAGATCCCTAATTTTGTATTTCCTGGAGGTGTTCGGCCTTCGCGCCCATCTAAAGTAAGTTGGGTTAGAAAGCGACGTTCAGAACTGGAGGTTTCTGGCAGCACTCAAGCAGATAGGTCAGGTGAAGATAGAACTGTTTCAGATGGAGCAGATAGTGGTAGAAAGAGAAAATTGGAGAATGATAATGTAGAGACTGACCATGCCAAGTCTATGCGTCCCTGCATTGGGGAAGTTTGTGAGGCTAGCCCTCCTTTTAGTAATGTCAGCCCATCCTGCGCAAAATATGAGAATAATGTAGAAGTTAGCACAGGAAAGATAGAAAGCCCAGCACAGATTCCCTATCAGAATGGTCAGGCTGAAGTATGGTCAAGGTGCAATCCATTAATCAACAGTCTATCTATTGCTGGTGATGCATCAAGTTCTAAAGAAGCAGAGAAACTGGCCTTAGAGAAGATTATGGCTGGTCCATATGTTTCACATCAAGCTTTGCCAGAAGAACTGGATGAGCTTGAAGGTGATTCTGAAGACAGGCATCAAGTCAAAGACTTTTCTGGGAACATGAAAGACAGTCGCATCGAGCCTTCAATTGAGAAATCTAACAATGGAGCTGAAACTTCCACTGGTTCATATAATGGAGGCTTAGAGGAACTTGAGCCGGCTGAACTTATGGCACCATCCTTGAATGGAACCCCCGAACCTGTGGCACAGAGGAAGCCAATCATCAGGCTGAACTTCACTTCCTTGCCCAATGCTTCTGGAAAGAGCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

738

Amino Acids

82.95

Weight (kDa)

6.02

Isoelectric Point (pI)

45.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	17 - 211	5.4e-78	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	86 - 158	4.8e-10	Nucleotidyltransferase domain
PAP_central	PF04928	216 - 360	8.6e-70	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	364 - 422	4.4e-12	Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind	PF04926	423 - 495	6e-06	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17980 AT1G17980
fragaria_vesca	FvH4_5g16820 FvH4_5g16820 FvH4_5g16820 FvH4_5g16820
malus_domestica	MD06G1222200.v1.1 MD09G1233900.v1.1 MD14G1231300.v1.1 MD17G1263100.v1.1
prunus_persica	Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1
pyrus_communis	pycom06g19770 pycom14g19250 pycom17g26340
rosa_chinensis	RchiOBHm_Chr7g0179251
rosa_laevigata	RLG00000005369
rosa_multiflora	Rmu_sc0006939.1_g000005
rosa_roxburghii	Rroxscaffold_3G00274180
rosa_rugosa	Rorug06G0422700 Rorug06G0422800 Rorug06G0422800
rosa_samantha	Rh7AG022000 Rh7BG021900 Rh7CG023200 Rh7DG022500
rosa_wichuraiana	Rw7G001810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1720
AccB1I	GGYRCC	1 cut(s)	2113
AccI	GTMKAC	2 cut(s)	1383, 1391
AccII	CGCG	1 cut(s)	1486
AciI	CCGC	2 cut(s)	75, 652
AclWI	GGATC	4 cut(s)	887, 900, 1411, 1441
AcoI	YGGCCR	2 cut(s)	34, 833
AcsI	RAATTY	3 cut(s)	618, 646, 1029
AcuI	CTGAAG	4 cut(s)	435, 793, 1821, 1986
AdeI	CACNNNGTG	1 cut(s)	341
AfaI	GTAC	1 cut(s)	988
AfiI	CCNNNNNNNGG	6 cut(s)	228, 235, 462, 1247, 1667, 1668
AgsI	TTSAA	9 cut(s)	244, 469, 705, 1097, 1201, 1372, 1955, 2028, 2125
AjnI	CCWGG	3 cut(s)	316, 1246, 1465
AjuI	GAANNNNNNNTTGG	4 cut(s)	165, 197, 363, 395
AleI	CACNNNNGTG	1 cut(s)	1248
AloI	GAACNNNNNNTCC	2 cut(s)	2165, 2197
Alw21I	GWGCWC	2 cut(s)	1341, 2210
Alw26I	GTCTC	4 cut(s)	596, 876, 1187, 1628
AlwI	GGATC	4 cut(s)	887, 900, 1411, 1441
Ama87I	CYCGRG	2 cut(s)	11, 363
AoxI	GGCC	6 cut(s)	34, 313, 833, 1297, 1478, 1884
ApeKI	GCWGC	4 cut(s)	40, 1133, 1228, 1542
ApoI	RAATTY	3 cut(s)	618, 646, 1029
ArsI	GACNNNNNNTTYG	4 cut(s)	610, 642, 870, 902
AseI	ATTAAT	1 cut(s)	1826
Asp700I	GAANNNNTTC	1 cut(s)	1579
AspLEI	GCGC	2 cut(s)	1488, 1721
AspS9I	GGNCC	5 cut(s)	77, 314, 347, 1297, 1907
AsuC2I	CCSGG	3 cut(s)	12, 13, 230
AsuHPI	GGTGA	7 cut(s)	395, 1229, 1262, 1346, 1577, 1862, 1970
AsuNHI	GCTAGC	1 cut(s)	1685
AvaI	CYCGRG	2 cut(s)	11, 363
AvaII	GGWCC	3 cut(s)	77, 347, 1907
AxyI	CCTNAGG	1 cut(s)	528
BalI	TGGCCA	2 cut(s)	36, 835
BamHI	GGATCC	1 cut(s)	892
BanI	GGYRCC	1 cut(s)	2113
BanII	GRGCYC	2 cut(s)	11, 2210
BbsI	GAAGAC	1 cut(s)	1974
Bbv12I	GWGCWC	2 cut(s)	1341, 2210
BbvI	GCAGC	4 cut(s)	52, 1145, 1215, 1554
BccI	CCATC	5 cut(s)	1139, 1498, 1580, 1720, 2125
BceAI	ACGGC	1 cut(s)	90
BcgI	CGANNNNNNTGC	2 cut(s)	763, 797
BciT130I	CCWGG	3 cut(s)	318, 1248, 1467
BciVI	GTATCC	2 cut(s)	1060, 1435
BclI	TGATCA	2 cut(s)	96, 193
BcnI	CCSGG	3 cut(s)	12, 13, 230
BcoDI	GTCTC	4 cut(s)	596, 876, 1187, 1628
BfaI	CTAG	6 cut(s)	302, 629, 794, 875, 966, 1686
BfmI	CTRYAG	2 cut(s)	601, 1229
BfuI	GTATCC	2 cut(s)	1060, 1435
BglII	AGATCT	1 cut(s)	276
BisI	GCNGC	4 cut(s)	41, 1134, 1229, 1543
BlsI	GCNGC	4 cut(s)	42, 1135, 1230, 1544
BmcAI	AGTACT	1 cut(s)	988
Bme1390I	CCNGG	6 cut(s)	12, 13, 230, 318, 1248, 1467
Bme18I	GGWCC	3 cut(s)	77, 347, 1907
BmeT110I	CYCGRG	2 cut(s)	11, 363
BmgT120I	GGNCC	5 cut(s)	77, 314, 347, 1297, 1907
BmiI	GGNNCC	5 cut(s)	8, 349, 894, 2115, 2131
BmrFI	CCNGG	6 cut(s)	12, 13, 230, 318, 1248, 1467
BmrI	ACTGGG	1 cut(s)	745
BmtI	GCTAGC	1 cut(s)	1689
BmuI	ACTGGG	1 cut(s)	745
BpiI	GAAGAC	1 cut(s)	1974
BpmI	CTGGAG	3 cut(s)	927, 1488, 1550
BpuEI	CTTGAG	4 cut(s)	134, 967, 1533, 2114
BpuMI	CCSGG	3 cut(s)	12, 13, 230
BsaBI	GATNNNNATC	1 cut(s)	1124
BsaI	GGTCTC	1 cut(s)	876
BsaJI	CCNNGG	5 cut(s)	10, 11, 316, 908, 1246
BsaWI	WCCGGW	2 cut(s)	24, 883
BsaXI	ACNNNNNCTCC	2 cut(s)	1308, 1338
Bsc4I	CCNNNNNNNGG	6 cut(s)	228, 235, 462, 1247, 1667, 1668
Bse118I	RCCGGY	1 cut(s)	2098
Bse1I	ACTGG	9 cut(s)	258, 740, 842, 1322, 1387, 1533, 1887, 1947, 2068
Bse21I	CCTNAGG	1 cut(s)	528
Bse3DI	GCAATG	2 cut(s)	740, 779
Bse8I	GATNNNNATC	1 cut(s)	1124
BseBI	CCWGG	3 cut(s)	318, 1248, 1467
BseDI	CCNNGG	5 cut(s)	10, 11, 316, 908, 1246
BseGI	GGATG	6 cut(s)	97, 402, 430, 1712, 1951, 2117
BseJI	GATNNNNATC	1 cut(s)	1124
BseLI	CCNNNNNNNGG	6 cut(s)	228, 235, 462, 1247, 1667, 1668
BseMI	GCAATG	2 cut(s)	740, 779
BseMII	CTCAG	3 cut(s)	48, 519, 836
BseNI	ACTGG	9 cut(s)	258, 740, 842, 1322, 1387, 1533, 1887, 1947, 2068
BseRI	GAGGAG	2 cut(s)	101, 1022
BseXI	GCAGC	4 cut(s)	52, 1145, 1215, 1554
BseYI	CCCAGC	1 cut(s)	1768
BsgI	GTGCAG	3 cut(s)	342, 588, 1152
Bsh1236I	CGCG	1 cut(s)	1486
BshFI	GGCC	6 cut(s)	36, 315, 835, 1299, 1480, 1886
BshNI	GGYRCC	1 cut(s)	2113
BsiHKAI	GWGCWC	2 cut(s)	1341, 2210
BsiHKCI	CYCGRG	2 cut(s)	11, 363
BsiSI	CCGG	5 cut(s)	12, 25, 229, 884, 2099
BslFI	GGGAC	4 cut(s)	617, 926, 1162, 1644
BslI	CCNNNNNNNGG	6 cut(s)	228, 235, 462, 1247, 1667, 1668
BsmAI	GTCTC	4 cut(s)	596, 876, 1187, 1628
BsmBI	CGTCTC	1 cut(s)	1187
BsmFI	GGGAC	4 cut(s)	617, 926, 1162, 1644
BsmI	GAATGC	2 cut(s)	639, 781
BsnI	GGCC	6 cut(s)	36, 315, 835, 1299, 1480, 1886
Bso31I	GGTCTC	1 cut(s)	876
BsoBI	CYCGRG	2 cut(s)	11, 363
Bsp1286I	GDGCHC	3 cut(s)	11, 1341, 2210
Bsp143I	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
BspACI	CCGC	2 cut(s)	75, 652
BspANI	GGCC	6 cut(s)	36, 315, 835, 1299, 1480, 1886
BspCNI	CTCAG	3 cut(s)	49, 520, 835
BspFNI	CGCG	1 cut(s)	1486
BspLI	GGNNCC	5 cut(s)	8, 349, 894, 2115, 2131
BspMAI	CTGCAG	1 cut(s)	1233
BspOI	GCTAGC	1 cut(s)	1689
BspPI	GGATC	4 cut(s)	887, 900, 1411, 1441
BspT107I	GGYRCC	1 cut(s)	2113
BspTNI	GGTCTC	1 cut(s)	876
BsrDI	GCAATG	2 cut(s)	740, 779
BsrFI	RCCGGY	1 cut(s)	2098
BsrI	ACTGG	9 cut(s)	258, 740, 842, 1322, 1387, 1533, 1887, 1947, 2068
BssAI	RCCGGY	1 cut(s)	2098
BssECI	CCNNGG	5 cut(s)	10, 11, 316, 908, 1246
BssMI	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
BssNAI	GTATAC	1 cut(s)	1392
BssT1I	CCWWGG	1 cut(s)	908
Bst1107I	GTATAC	1 cut(s)	1392
Bst2UI	CCWGG	3 cut(s)	318, 1248, 1467
Bst4CI	ACNGT	4 cut(s)	583, 1579, 1835, 2012
Bst6I	CTCTTC	2 cut(s)	150, 168
BstAPI	GCANNNNNTGC	1 cut(s)	1139
BstC8I	GCNNGC	6 cut(s)	38, 188, 754, 1377, 1687, 2100
BstDEI	CTNAG	8 cut(s)	57, 273, 528, 535, 662, 822, 1888, 2083
BstF5I	GGATG	6 cut(s)	97, 402, 430, 1712, 1951, 2117
BstFNI	CGCG	1 cut(s)	1486
BstHHI	GCGC	2 cut(s)	1488, 1721
BstKTI	GATC	7 cut(s)	99, 196, 279, 493, 895, 1419, 1449
BstMAI	GTCTC	4 cut(s)	596, 876, 1187, 1628
BstMBI	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
BstMWI	GCNNNNNNNGC	7 cut(s)	187, 790, 1046, 1055, 1139, 1716, 1853
BstNI	CCWGG	3 cut(s)	318, 1248, 1467
BstNSI	RCATGY	1 cut(s)	359
BstSCI	CCNGG	6 cut(s)	10, 11, 228, 316, 1246, 1465
BstSFI	CTRYAG	2 cut(s)	601, 1229
BstUI	CGCG	1 cut(s)	1486
BstV1I	GCAGC	4 cut(s)	52, 1145, 1215, 1554
BstV2I	GAAGAC	1 cut(s)	1974
BstX2I	RGATCY	4 cut(s)	276, 892, 1416, 1446
BstYI	RGATCY	4 cut(s)	276, 892, 1416, 1446
BstZ17I	GTATAC	1 cut(s)	1392
Bsu36I	CCTNAGG	1 cut(s)	528
BsuI	GTATCC	2 cut(s)	1060, 1435
BsuRI	GGCC	6 cut(s)	36, 315, 835, 1299, 1480, 1886
BtsCI	GGATG	6 cut(s)	97, 402, 430, 1712, 1951, 2117
BtsI	GCAGTG	1 cut(s)	1238
BtsIMutI	CAGTG	4 cut(s)	830, 1135, 1238, 2061
Cac8I	GCNNGC	6 cut(s)	38, 188, 754, 1377, 1687, 2100
CfoI	GCGC	2 cut(s)	1488, 1721
Cfr10I	RCCGGY	1 cut(s)	2098
Cfr13I	GGNCC	5 cut(s)	77, 314, 347, 1297, 1907
Cfr9I	CCCGGG	1 cut(s)	11
CpoI	CGGWCCG	1 cut(s)	77
CseI	GACGC	4 cut(s)	140, 614, 677, 1646
Csp6I	GTAC	1 cut(s)	987
CspI	CGGWCCG	1 cut(s)	77
CviQI	GTAC	1 cut(s)	987
DdeI	CTNAG	8 cut(s)	57, 273, 528, 535, 662, 822, 1888, 2083
DpnI	GATC	7 cut(s)	98, 195, 278, 492, 894, 1418, 1448
DpnII	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
DraIII	CACNNNGTG	1 cut(s)	341
EaeI	YGGCCR	2 cut(s)	34, 833
Eam1104I	CTCTTC	2 cut(s)	150, 168
EarI	CTCTTC	2 cut(s)	150, 168
Ecl136II	GAGCTC	1 cut(s)	2208
Eco130I	CCWWGG	1 cut(s)	908
Eco24I	GRGCYC	2 cut(s)	11, 2210
Eco31I	GGTCTC	1 cut(s)	876
Eco32I	GATATC	1 cut(s)	920
Eco47I	GGWCC	3 cut(s)	77, 347, 1907
Eco53kI	GAGCTC	1 cut(s)	2208
Eco57I	CTGAAG	4 cut(s)	435, 793, 1821, 1986
Eco81I	CCTNAGG	1 cut(s)	528
Eco88I	CYCGRG	2 cut(s)	11, 363
EcoICRI	GAGCTC	1 cut(s)	2208
EcoRII	CCWGG	3 cut(s)	316, 1246, 1465
EcoRV	GATATC	1 cut(s)	920
EcoT14I	CCWWGG	1 cut(s)	908
EcoT22I	ATGCAT	3 cut(s)	671, 781, 1858
EcoT38I	GRGCYC	2 cut(s)	11, 2210
ErhI	CCWWGG	1 cut(s)	908
Esp3I	CGTCTC	1 cut(s)	1187
FalI	AAGNNNNNCTT	2 cut(s)	165, 197
FaqI	GGGAC	4 cut(s)	617, 926, 1162, 1644
FauNDI	CATATG	2 cut(s)	758, 1912
FbaI	TGATCA	2 cut(s)	96, 193
FblI	GTMKAC	2 cut(s)	1383, 1391
Fnu4HI	GCNGC	4 cut(s)	41, 1134, 1229, 1543
FokI	GGATG	6 cut(s)	104, 409, 417, 1699, 1958, 2104
FriOI	GRGCYC	2 cut(s)	11, 2210
Fsp4HI	GCNGC	4 cut(s)	41, 1134, 1229, 1543
FspBI	CTAG	6 cut(s)	302, 629, 794, 875, 966, 1686
FspI	TGCGCA	1 cut(s)	1720
GlaI	GCGC	2 cut(s)	1487, 1720
GluI	GCNGC	4 cut(s)	41, 1134, 1229, 1543
GsaI	CCCAGC	1 cut(s)	1772
GsuI	CTGGAG	3 cut(s)	927, 1488, 1550
HaeIII	GGCC	6 cut(s)	36, 315, 835, 1299, 1480, 1886
HapII	CCGG	5 cut(s)	12, 25, 229, 884, 2099
HgaI	GACGC	4 cut(s)	140, 614, 677, 1646
HhaI	GCGC	2 cut(s)	1488, 1721
Hin6I	GCGC	2 cut(s)	1486, 1719
HinP1I	GCGC	2 cut(s)	1486, 1719
HincII	GTYRAC	1 cut(s)	1190
HindII	GTYRAC	1 cut(s)	1190
HindIII	AAGCTT	2 cut(s)	1373, 1926
HinfI	GANTC	5 cut(s)	148, 1079, 1175, 1777, 1961
HpaII	CCGG	5 cut(s)	12, 25, 229, 884, 2099
HphI	GGTGA	7 cut(s)	395, 1229, 1262, 1346, 1577, 1862, 1970
Hpy166II	GTNNAC	3 cut(s)	1190, 1384, 1392
Hpy188III	TCNNGA	8 cut(s)	152, 365, 440, 527, 714, 1259, 1269, 2199
Hpy8I	GTNNAC	3 cut(s)	1190, 1384, 1392
Hpy99I	CGWCG	3 cut(s)	85, 693, 1521
HpyAV	CCTTC	6 cut(s)	229, 860, 1325, 1491, 1949, 2034
HpyCH4III	ACNGT	4 cut(s)	583, 1579, 1835, 2012
HpyCH4IV	ACGT	2 cut(s)	284, 1519
HpyF10VI	GCNNNNNNNGC	7 cut(s)	187, 790, 1046, 1055, 1139, 1716, 1853
HpyF3I	CTNAG	8 cut(s)	57, 273, 528, 535, 662, 822, 1888, 2083
HpySE526I	ACGT	2 cut(s)	284, 1519
HspAI	GCGC	2 cut(s)	1486, 1719
KroI	GCCGGC	1 cut(s)	2098
KroNI	GCCGGC	1 cut(s)	2100
Ksp22I	TGATCA	2 cut(s)	96, 193
Kzo9I	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
LmnI	GCTCC	3 cut(s)	6, 1589, 2048
Lsp1109I	GCAGC	4 cut(s)	52, 1145, 1215, 1554
MaeI	CTAG	6 cut(s)	302, 629, 794, 875, 966, 1686
MaeII	ACGT	2 cut(s)	284, 1519
MaeIII	GTNAC	4 cut(s)	387, 418, 607, 1235
MalI	GATC	7 cut(s)	98, 195, 278, 492, 894, 1418, 1448
MboI	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
MfeI	CAATTG	2 cut(s)	486, 2028
MflI	RGATCY	4 cut(s)	276, 892, 1416, 1446
MhlI	GDGCHC	3 cut(s)	11, 1341, 2210
MlsI	TGGCCA	2 cut(s)	36, 835
MluNI	TGGCCA	2 cut(s)	36, 835
MlyI	GAGTC	3 cut(s)	157, 1088, 1184
MmeI	TCCRAC	2 cut(s)	63, 325
Mox20I	TGGCCA	2 cut(s)	36, 835
Mph1103I	ATGCAT	3 cut(s)	671, 781, 1858
MroNI	GCCGGC	1 cut(s)	2098
MroXI	GAANNNNTTC	1 cut(s)	1579
MscI	TGGCCA	2 cut(s)	36, 835
MseI	TTAA	6 cut(s)	17, 216, 474, 1349, 1397, 1826
MslI	CAYNNNNRTG	2 cut(s)	664, 1248
Msp20I	TGGCCA	2 cut(s)	36, 835
MspI	CCGG	5 cut(s)	12, 25, 229, 884, 2099
MspR9I	CCNGG	6 cut(s)	12, 13, 230, 318, 1248, 1467
MunI	CAATTG	2 cut(s)	486, 2028
Mva1269I	GAATGC	2 cut(s)	639, 781
MvaI	CCWGG	3 cut(s)	318, 1248, 1467
MvnI	CGCG	1 cut(s)	1486
MwoI	GCNNNNNNNGC	7 cut(s)	187, 790, 1046, 1055, 1139, 1716, 1853
NaeI	GCCGGC	1 cut(s)	2100
NciI	CCSGG	3 cut(s)	12, 13, 230
NdeI	CATATG	2 cut(s)	758, 1912
NdeII	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
NgoMIV	GCCGGC	1 cut(s)	2098
NheI	GCTAGC	1 cut(s)	1685
NlaIV	GGNNCC	5 cut(s)	8, 349, 894, 2115, 2131
NmuCI	GTSAC	1 cut(s)	1235
NsbI	TGCGCA	1 cut(s)	1720
NsiI	ATGCAT	3 cut(s)	671, 781, 1858
NspI	RCATGY	1 cut(s)	359
OliI	CACNNNNGTG	1 cut(s)	1248
PaeR7I	CTCGAG	1 cut(s)	363
PcsI	WCGNNNNNNNCGW	1 cut(s)	290
PctI	GAATGC	2 cut(s)	639, 781
PdiI	GCCGGC	1 cut(s)	2100
PdmI	GAANNNNTTC	1 cut(s)	1579
PfeI	GAWTC	2 cut(s)	1777, 1961
PfoI	TCCNGGA	1 cut(s)	1465
PkrI	GCNGC	4 cut(s)	42, 1135, 1230, 1544
PleI	GAGTC	3 cut(s)	156, 1087, 1183
PpsI	GAGTC	3 cut(s)	156, 1087, 1183
PshBI	ATTAAT	1 cut(s)	1826
Psp124BI	GAGCTC	1 cut(s)	2210
Psp6I	CCWGG	3 cut(s)	316, 1246, 1465
PspFI	CCCAGC	1 cut(s)	1768
PspGI	CCWGG	3 cut(s)	316, 1246, 1465
PspN4I	GGNNCC	5 cut(s)	8, 349, 894, 2115, 2131
PspPI	GGNCC	5 cut(s)	77, 314, 347, 1297, 1907
PsrI	GAACNNNNNNTAC	2 cut(s)	953, 985
PstI	CTGCAG	1 cut(s)	1233
PsuI	RGATCY	4 cut(s)	276, 892, 1416, 1446
RsaI	GTAC	1 cut(s)	988
RsaNI	GTAC	1 cut(s)	987
RseI	CAYNNNNRTG	2 cut(s)	664, 1248
Rsr2I	CGGWCCG	1 cut(s)	77
RsrII	CGGWCCG	1 cut(s)	77
SacI	GAGCTC	1 cut(s)	2210
SaqAI	TTAA	6 cut(s)	17, 216, 474, 1349, 1397, 1826
SatI	GCNGC	4 cut(s)	41, 1134, 1229, 1543
Sau3AI	GATC	7 cut(s)	96, 193, 276, 490, 892, 1416, 1446
Sau96I	GGNCC	5 cut(s)	77, 314, 347, 1297, 1907
ScaI	AGTACT	1 cut(s)	988
SchI	GAGTC	3 cut(s)	157, 1088, 1184
ScrFI	CCNGG	6 cut(s)	12, 13, 230, 318, 1248, 1467
SduI	GDGCHC	3 cut(s)	11, 1341, 2210
SfcI	CTRYAG	2 cut(s)	601, 1229
Sfr274I	CTCGAG	1 cut(s)	363
SinI	GGWCC	3 cut(s)	77, 347, 1907
SlaI	CTCGAG	1 cut(s)	363
SmaI	CCCGGG	1 cut(s)	13
SmiMI	CAYNNNNRTG	2 cut(s)	664, 1248
SmlI	CTYRAG	5 cut(s)	113, 363, 982, 1548, 2093
SmoI	CTYRAG	5 cut(s)	113, 363, 982, 1548, 2093
SsiI	CCGC	2 cut(s)	75, 652
SspMI	CTAG	6 cut(s)	302, 629, 794, 875, 966, 1686
SstI	GAGCTC	1 cut(s)	2210
StyD4I	CCNGG	6 cut(s)	10, 11, 228, 316, 1246, 1465
StyI	CCWWGG	1 cut(s)	908
TaaI	ACNGT	4 cut(s)	583, 1579, 1835, 2012
TaiI	ACGT	2 cut(s)	287, 1522
TaqI	TCGA	3 cut(s)	364, 861, 2019
TatI	WGTACW	1 cut(s)	986
TfiI	GAWTC	2 cut(s)	1777, 1961
Tru1I	TTAA	6 cut(s)	17, 216, 474, 1349, 1397, 1826
Tru9I	TTAA	6 cut(s)	17, 216, 474, 1349, 1397, 1826
TscAI	CASTG	4 cut(s)	830, 1135, 1238, 2068
TseFI	GTSAC	1 cut(s)	1235
TseI	GCWGC	4 cut(s)	40, 1133, 1228, 1542
Tsp45I	GTSAC	1 cut(s)	1235
TspGWI	ACGGA	5 cut(s)	69, 437, 492, 1175, 1409
TspMI	CCCGGG	1 cut(s)	11
TspRI	CASTG	4 cut(s)	830, 1135, 1238, 2068
VpaK11BI	GGWCC	3 cut(s)	77, 347, 1907
VspI	ATTAAT	1 cut(s)	1826
XapI	RAATTY	3 cut(s)	618, 646, 1029
XceI	RCATGY	1 cut(s)	359
XhoI	CTCGAG	1 cut(s)	363
XmaI	CCCGGG	1 cut(s)	11
XmiI	GTMKAC	2 cut(s)	1383, 1391
XmnI	GAANNNNTTC	1 cut(s)	1579
XspI	CTAG	6 cut(s)	302, 629, 794, 875, 966, 1686
ZrmI	AGTACT	1 cut(s)	988
Zsp2I	ATGCAT	3 cut(s)	671, 781, 1858

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.