Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005853

Physical Location & Seq

Genomic Coordinates Reverse (-)

31189895 .. 31195479

Gene Structure 5585 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1231300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2358 bp

ATGGGGAGCCCTGCATTAAGCAACCGAAATAATGGGCAGCGGTTAGGCATTACAGAGCCCATTTCTCTGGGCGGTCCGACGGAGTATGATGTGATCAAAACTCGAGAACTCGAAAAGTATTTGCAAGATGCTGGATTGTACGAGAGTCAGGAAGAGGCTGTGGGTAGGGAGGAGGTTCTTGGCAGGCTAGACCAGATTGTGAAGACGTGGGTTAAAACAATTAGCCGGACGAAGGGTTTGAACGAGCAATTGGTGCATGAAGCAAATGCTAAGATTTTCACCTTCGGTTCATATCGGCTAGGGGTTCATGGCCCTGGAGCAGATATAGACACACTCTGTGTTGGACCTAGACATGCAACTCGAGAAGGAGATTTCTTTGGCGAGTTACATAGGATGCTGTCTGAGATGTCTGAAGTGACAGAGTTGCATCCCGTTCCTGATGCTCACGTCCCAGTTATGAAATTTAAGTTCAGTGGGGTTTCAATAGATCTTCTTTATGCACAACTGGCACTTTGGGTTGTTCCTGAAGACTTGGATATATCACAAGACTCAATACTACAGAATGCAGATGAACAGACAGTTCGTAGTCTCAATGGCTGCAGAGTTACAGACCAAATTCTGCGTTTAGTCCCTAACATTCAGAGTTTCCGCACAACATTAAGATGCATGAGGTTATGGGCAAAGCGTCGTGGTGTTTATTCAAATGTTACAGGGTTTCTTGGTGGTATAAACTGGGCATTGCTTGTTGCTCGCATATGCCAACTTTACCCGAATGCACTGCCTAATATGCTAGTTTCTCGGTTCTTCAGGGTATATACTCAGTGGCGTTGGCCAAACCCAGTCATGCTTTGTGCTATTGAAGATGGGTCTCTTGGACTTCAGGTTTGGGATCCTAGAAGATATATAAAGGACAAGTATCATTTGATGCCTATAATTACTCCGGCTTATCCCAGTATGAACTCTAGCTACAATGTGTCCGCAAGTACTTTGCGTATCATGTTAGAGGAGTTTCAGAGGGGTAATGAAATCTGTGAGGCTATGGAAGCAAATAAGGCTGATTGGGATACCCTTTTTGAGTCTTATGCTTTCTTTGAAGCATATAAGAATTATTTACAGATAGACATCAGTGCAGAAAATGCTGATGACTTTAGAAAATGGAAAGGCTGGGTTGAGTCTCGTCTCCGTCAACTAACCCTGAAGATTGAGAGGTACACATATGACATGCTGCAGTGTCACCCGCATCCAGGTGATTTTTCAGACAGAACGAGACCTTTTCATAGTTCGTTCTTTATGGGCCTACAACGAAAACAAGGAGTTCCAGTCACTGAAGGCGAGCAGTTTGACATAAGGGGAACTGTTGAGGAATTTAAGAATGCTGTCAACCATTACACGTTATTGAAGCCTGGAATGGAGATCCGTGTATCCCATGTAAAACGCAGAAACATTCCTAATTTTGTGTTTCCTGGTGGTGTTCGACCATTGCGCCCATCAAAAGTTACTTGGGACAGCAGGCGTAGTTCAGAACTGAAGGCTTCTGGTGATGCTCAAGCAGAAAAATCATGTGAAGGAAATGGATCAGACAGTGGACAGAAGAGAAAGCGGGTGGATGATAATGTAGAGTCTGACTCAAGGGATGCCAAGTCTTTACGTCTCTTTAGTGGGGAAGGTAAAACTGATTTAGATGGATCAGACAGTGGACAGAACAGAAAGCGGGTGGATGATAATGTAGAAACTGACTCAAGGCATGCCAAGTCTTTACATCTCTGCAGTGATGAAGTTTGTGAGGCTACCCCTCCTATCAGCAATTTCAGTTCATTTTCCACAAAATGTGACAGTATCGATGCAAACAAAAATGACAATAATAACGTGAAAATTAGCTTAGGGAAGATAGAAAACCCGGCAGACATTCCTTATCAGAATGGTCAGACTGAAGTATCGTCAAGGTGCAATCCACCAGTCAATAGTCTATTTTCTGCTGTGAATACATCAAGTTCTAAAGAAGAGGTACTAGAGAAGATTATGGTCGGTCCATGTGTTTCCCACCAAGCTTTGCCAGAAGTGGATGAGCTTGAAGATGATTTGGAATATAGACATCAAGTCAAGGACCTTTCAGGAAATATGAAGGTCAGTCAAATGGAGGCTTCGGAGGAGAGTGTGTCAGTTGCAACGCCAGTAAGTTATAGCAATGGAGCTGGCACTTCCACCGATTTATATAATGGAGGATCAGAGGAACTTGAGCCTGCTGAACTTACGGCGCCATTCTCAAACGGAACCCCTTCTCCGCCTGTGGCTCAGAGAAAGCCGATCATCAGGTTGAATTTCACTTCATTGGCCAAAGCTGCTGGTAAAAGCTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

786

Amino Acids

88.0

Weight (kDa)

5.73

Isoelectric Point (pI)

44.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	16 - 210	4.2e-81	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	86 - 164	2.9e-10	Nucleotidyltransferase domain
PAP_central	PF04928	215 - 359	2.4e-69	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	363 - 421	9.8e-13	Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind	PF04926	422 - 492	3.4e-06	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17980 AT1G17980
fragaria_vesca	FvH4_5g16820 FvH4_5g16820 FvH4_5g16820 FvH4_5g16820
malus_domestica	MD06G1222200.v1.1 MD09G1233900.v1.1 MD14G1231300.v1.1 MD17G1263100.v1.1
prunus_persica	Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1
pyrus_communis	pycom06g19770 pycom14g19250 pycom17g26340
rosa_chinensis	RchiOBHm_Chr7g0179251
rosa_laevigata	RLG00000005369
rosa_multiflora	Rmu_sc0006939.1_g000005
rosa_roxburghii	Rroxscaffold_3G00274180
rosa_rugosa	Rorug06G0422700 Rorug06G0422800 Rorug06G0422800
rosa_samantha	Rh7AG022000 Rh7BG021900 Rh7CG023200 Rh7DG022500
rosa_wichuraiana	Rw7G001810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2254
AciI	CCGC	8 cut(s)	40, 72, 649, 978, 1238, 1600, 1711, 2282
AclWI	GGATC	6 cut(s)	884, 897, 1408, 1582, 1693, 2230
AcoI	YGGCCR	2 cut(s)	830, 2331
AcsI	RAATTY	4 cut(s)	461, 615, 1364, 2317
AcuI	CTGAAG	8 cut(s)	432, 546, 790, 863, 1217, 1347, 1547, 1950
AcyI	GRCGYC	1 cut(s)	2255
AdeI	CACNNNGTG	1 cut(s)	338
AfaI	GTAC	4 cut(s)	140, 985, 1211, 2007
AfiI	CCNNNNNNNGG	2 cut(s)	232, 1244
AflIII	ACRYGT	1 cut(s)	1389
AgsI	TTSAA	8 cut(s)	241, 483, 702, 860, 1094, 1399, 2072, 2317
AhdI	GACNNNNNGTC	1 cut(s)	2096
AjiI	CACGTC	2 cut(s)	207, 448
AjnI	CCWGG	4 cut(s)	313, 1243, 1402, 1462
AjuI	GAANNNNNNNTTGG	2 cut(s)	233, 265
AloI	GAACNNNNNNTCC	2 cut(s)	2263, 2295
AluBI	AGCT	7 cut(s)	966, 1878, 2048, 2068, 2192, 2339, 2352
AluI	AGCT	7 cut(s)	966, 1878, 2048, 2068, 2192, 2339, 2352
Alw26I	GTCTC	6 cut(s)	593, 873, 1179, 1184, 1261, 1655
AlwI	GGATC	6 cut(s)	884, 897, 1408, 1582, 1693, 2230
AlwNI	CAGNNNCTG	1 cut(s)	1325
Ama87I	CYCGRG	2 cut(s)	102, 360
AoxI	GGCC	4 cut(s)	310, 830, 1294, 2331
ApeKI	GCWGC	4 cut(s)	37, 597, 1225, 2339
ApoI	RAATTY	4 cut(s)	461, 615, 1364, 2317
ArsI	GACNNNNNNTTYG	4 cut(s)	220, 252, 867, 899
Asp700I	GAANNNNTTC	1 cut(s)	2275
AspLEI	GCGC	2 cut(s)	1485, 2257
AspS9I	GGNCC	6 cut(s)	74, 311, 344, 1294, 2027, 2104
AsuC2I	CCSGG	1 cut(s)	1898
AsuHPI	GGTGA	4 cut(s)	271, 1226, 1259, 1550
AvaI	CYCGRG	2 cut(s)	102, 360
AvaII	GGWCC	4 cut(s)	74, 344, 2027, 2104
BaeI	ACNNNNGTAYC	2 cut(s)	1917, 1950
BalI	TGGCCA	2 cut(s)	832, 2333
BamHI	GGATCC	1 cut(s)	889
BanI	GGYRCC	1 cut(s)	2254
BanII	GRGCYC	2 cut(s)	11, 60
BbsI	GAAGAC	2 cut(s)	209, 534
BbvI	GCAGC	4 cut(s)	49, 584, 1212, 2326
BccI	CCATC	3 cut(s)	857, 1495, 1676
BceAI	ACGGC	1 cut(s)	2268
BcgI	CGANNNNNNTGC	2 cut(s)	760, 794
BciT130I	CCWGG	4 cut(s)	315, 1245, 1404, 1464
BciVI	GTATCC	2 cut(s)	1057, 1432
BclI	TGATCA	1 cut(s)	93
BcnI	CCSGG	1 cut(s)	1898
BcoDI	GTCTC	6 cut(s)	593, 873, 1179, 1184, 1261, 1655
BfaI	CTAG	7 cut(s)	188, 299, 348, 791, 894, 963, 2009
BfmI	CTRYAG	4 cut(s)	557, 598, 1226, 1765
BfoI	RGCGCY	1 cut(s)	2258
BfuI	GTATCC	2 cut(s)	1057, 1432
BglII	AGATCT	1 cut(s)	487
BisI	GCNGC	4 cut(s)	38, 598, 1226, 2340
BlsI	GCNGC	4 cut(s)	39, 599, 1227, 2341
BmcAI	AGTACT	1 cut(s)	985
Bme1390I	CCNGG	5 cut(s)	315, 1245, 1404, 1464, 1898
Bme18I	GGWCC	4 cut(s)	74, 344, 2027, 2104
BmeRI	GACNNNNNGTC	1 cut(s)	2096
BmeT110I	CYCGRG	2 cut(s)	102, 360
BmgBI	CACGTC	2 cut(s)	207, 448
BmgT120I	GGNCC	6 cut(s)	74, 311, 344, 1294, 2027, 2104
BmiI	GGNNCC	4 cut(s)	8, 891, 2256, 2272
BmrFI	CCNGG	5 cut(s)	315, 1245, 1404, 1464, 1898
BmrI	ACTGGG	4 cut(s)	446, 742, 833, 945
BmuI	ACTGGG	4 cut(s)	446, 742, 833, 945
BpiI	GAAGAC	2 cut(s)	209, 534
BplI	GAGNNNNNCTC	2 cut(s)	1610, 1642
BpmI	CTGGAG	1 cut(s)	336
Bpu10I	CCTNAGC	1 cut(s)	1879
BpuEI	CTTGAG	4 cut(s)	1530, 1612, 1723, 2255
BpuMI	CCSGG	1 cut(s)	1898
Bsa29I	ATCGAT	1 cut(s)	1839
BsaBI	GATNNNNATC	1 cut(s)	1121
BsaHI	GRCGYC	1 cut(s)	2255
BsaI	GGTCTC	2 cut(s)	873, 1261
BsaJI	CCNNGG	1 cut(s)	313
BsaXI	ACNNNNNCTCC	6 cut(s)	1305, 1335, 2141, 2171, 2263, 2293
Bsc4I	CCNNNNNNNGG	2 cut(s)	232, 1244
Bse1I	ACTGG	8 cut(s)	452, 510, 737, 839, 951, 1319, 1955, 2171
Bse3DI	GCAATG	3 cut(s)	737, 1478, 2191
Bse8I	GATNNNNATC	1 cut(s)	1121
BseBI	CCWGG	4 cut(s)	315, 1245, 1404, 1464
BseCI	ATCGAT	1 cut(s)	1839
BseDI	CCNNGG	1 cut(s)	313
BseGI	GGATG	7 cut(s)	399, 427, 1240, 1612, 1639, 1723, 2068
BseJI	GATNNNNATC	1 cut(s)	1121
BseLI	CCNNNNNNNGG	2 cut(s)	232, 1244
BseMI	GCAATG	3 cut(s)	737, 1478, 2191
BseMII	CTCAG	3 cut(s)	393, 833, 2306
BseNI	ACTGG	8 cut(s)	452, 510, 737, 839, 951, 1319, 1955, 2171
BseRI	GAGGAG	3 cut(s)	185, 1019, 2162
BseXI	GCAGC	4 cut(s)	49, 584, 1212, 2326
BseYI	CCCAGC	1 cut(s)	1164
BsgI	GTGCAG	1 cut(s)	1149
BshFI	GGCC	4 cut(s)	312, 832, 1296, 2333
BshNI	GGYRCC	1 cut(s)	2254
BshVI	ATCGAT	1 cut(s)	1839
BsiHKCI	CYCGRG	2 cut(s)	102, 360
BsiSI	CCGG	3 cut(s)	226, 941, 1898
BslFI	GGGAC	3 cut(s)	434, 614, 1517
BslI	CCNNNNNNNGG	2 cut(s)	232, 1244
BsmAI	GTCTC	6 cut(s)	593, 873, 1179, 1184, 1261, 1655
BsmBI	CGTCTC	2 cut(s)	1184, 1655
BsmFI	GGGAC	3 cut(s)	434, 614, 1517
BsmI	GAATGC	3 cut(s)	568, 778, 1378
BsnI	GGCC	4 cut(s)	312, 832, 1296, 2333
Bso31I	GGTCTC	2 cut(s)	873, 1261
BsoBI	CYCGRG	2 cut(s)	102, 360
Bsp1286I	GDGCHC	2 cut(s)	11, 60
Bsp143I	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
BspACI	CCGC	8 cut(s)	40, 72, 649, 978, 1238, 1600, 1711, 2282
BspANI	GGCC	4 cut(s)	312, 832, 1296, 2333
BspCNI	CTCAG	3 cut(s)	394, 832, 2305
BspDI	ATCGAT	1 cut(s)	1839
BspLI	GGNNCC	4 cut(s)	8, 891, 2256, 2272
BspMAI	CTGCAG	3 cut(s)	602, 1230, 1769
BspPI	GGATC	6 cut(s)	884, 897, 1408, 1582, 1693, 2230
BspT107I	GGYRCC	1 cut(s)	2254
BspTNI	GGTCTC	2 cut(s)	873, 1261
BsrDI	GCAATG	3 cut(s)	737, 1478, 2191
BsrI	ACTGG	8 cut(s)	452, 510, 737, 839, 951, 1319, 1955, 2171
BssECI	CCNNGG	1 cut(s)	313
BssMI	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
BssNI	GRCGYC	1 cut(s)	2255
Bst2UI	CCWGG	4 cut(s)	315, 1245, 1404, 1464
Bst4CI	ACNGT	5 cut(s)	580, 1357, 1583, 1694, 1835
Bst6I	CTCTTC	3 cut(s)	147, 1586, 1995
BstACI	GRCGYC	1 cut(s)	2255
BstAPI	GCANNNNNTGC	2 cut(s)	253, 1136
BstC8I	GCNNGC	7 cut(s)	185, 751, 1334, 1511, 1746, 2194, 2241
BstDEI	CTNAG	5 cut(s)	270, 402, 819, 1879, 2292
BstF5I	GGATG	7 cut(s)	399, 427, 1240, 1612, 1639, 1723, 2068
BstH2I	RGCGCY	1 cut(s)	2258
BstHHI	GCGC	2 cut(s)	1485, 2257
BstKTI	GATC	8 cut(s)	96, 490, 892, 1416, 1577, 1688, 2225, 2307
BstMAI	GTCTC	6 cut(s)	593, 873, 1179, 1184, 1261, 1655
BstMBI	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
BstMWI	GCNNNNNNNGC	7 cut(s)	253, 506, 787, 1043, 1052, 1136, 2339
BstNI	CCWGG	4 cut(s)	315, 1245, 1404, 1464
BstNSI	RCATGY	3 cut(s)	356, 1225, 1748
BstSCI	CCNGG	5 cut(s)	313, 1243, 1402, 1462, 1896
BstSFI	CTRYAG	4 cut(s)	557, 598, 1226, 1765
BstV1I	GCAGC	4 cut(s)	49, 584, 1212, 2326
BstV2I	GAAGAC	2 cut(s)	209, 534
BstX2I	RGATCY	3 cut(s)	487, 889, 1413
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstYI	RGATCY	3 cut(s)	487, 889, 1413
Bsu15I	ATCGAT	1 cut(s)	1839
BsuI	GTATCC	2 cut(s)	1057, 1432
BsuRI	GGCC	4 cut(s)	312, 832, 1296, 2333
BsuTUI	ATCGAT	1 cut(s)	1839
BtrI	CACGTC	2 cut(s)	207, 448
BtsCI	GGATG	7 cut(s)	399, 427, 1240, 1612, 1639, 1723, 2068
BtsI	GCAGTG	3 cut(s)	776, 1235, 1774
BtsIMutI	CAGTG	9 cut(s)	478, 776, 827, 1132, 1235, 1323, 1588, 1699, 1774
Cac8I	GCNNGC	7 cut(s)	185, 751, 1334, 1511, 1746, 2194, 2241
CaiI	CAGNNNCTG	1 cut(s)	1325
CfoI	GCGC	2 cut(s)	1485, 2257
Cfr13I	GGNCC	6 cut(s)	74, 311, 344, 1294, 2027, 2104
ClaI	ATCGAT	1 cut(s)	1839
CpoI	CGGWCCG	1 cut(s)	74
CseI	GACGC	1 cut(s)	674
Csp6I	GTAC	4 cut(s)	139, 984, 1210, 2006
CspI	CGGWCCG	1 cut(s)	74
CviQI	GTAC	4 cut(s)	139, 984, 1210, 2006
DdeI	CTNAG	5 cut(s)	270, 402, 819, 1879, 2292
DinI	GGCGCC	1 cut(s)	2256
DpnI	GATC	8 cut(s)	95, 489, 891, 1415, 1576, 1687, 2224, 2306
DpnII	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
DraIII	CACNNNGTG	1 cut(s)	338
DriI	GACNNNNNGTC	1 cut(s)	2096
EaeI	YGGCCR	2 cut(s)	830, 2331
Eam1104I	CTCTTC	3 cut(s)	147, 1586, 1995
Eam1105I	GACNNNNNGTC	1 cut(s)	2096
EarI	CTCTTC	3 cut(s)	147, 1586, 1995
EciI	GGCGGA	1 cut(s)	2271
Eco24I	GRGCYC	2 cut(s)	11, 60
Eco31I	GGTCTC	2 cut(s)	873, 1261
Eco47I	GGWCC	4 cut(s)	74, 344, 2027, 2104
Eco57I	CTGAAG	8 cut(s)	432, 546, 790, 863, 1217, 1347, 1547, 1950
Eco88I	CYCGRG	2 cut(s)	102, 360
EcoO109I	RGGNCCY	1 cut(s)	2104
EcoRII	CCWGG	4 cut(s)	313, 1243, 1402, 1462
EcoT22I	ATGCAT	1 cut(s)	668
EcoT38I	GRGCYC	2 cut(s)	11, 60
EgeI	GGCGCC	1 cut(s)	2256
EheI	GGCGCC	1 cut(s)	2256
Esp3I	CGTCTC	2 cut(s)	1184, 1655
FaqI	GGGAC	3 cut(s)	434, 614, 1517
FauI	CCCGC	3 cut(s)	1245, 1593, 1704
FauNDI	CATATG	2 cut(s)	755, 1216
FbaI	TGATCA	1 cut(s)	93
Fnu4HI	GCNGC	4 cut(s)	38, 598, 1226, 2340
FokI	GGATG	7 cut(s)	406, 414, 1227, 1619, 1646, 1730, 2075
FriOI	GRGCYC	2 cut(s)	11, 60
Fsp4HI	GCNGC	4 cut(s)	38, 598, 1226, 2340
FspBI	CTAG	7 cut(s)	188, 299, 348, 791, 894, 963, 2009
GlaI	GCGC	2 cut(s)	1484, 2256
GluI	GCNGC	4 cut(s)	38, 598, 1226, 2340
GsaI	CCCAGC	1 cut(s)	1168
GsuI	CTGGAG	1 cut(s)	336
HaeII	RGCGCY	1 cut(s)	2258
HaeIII	GGCC	4 cut(s)	312, 832, 1296, 2333
HapII	CCGG	3 cut(s)	226, 941, 1898
HgaI	GACGC	1 cut(s)	674
HhaI	GCGC	2 cut(s)	1485, 2257
Hin1I	GRCGYC	1 cut(s)	2255
Hin6I	GCGC	2 cut(s)	1483, 2255
HinP1I	GCGC	2 cut(s)	1483, 2255
HincII	GTYRAC	2 cut(s)	1187, 1381
HindII	GTYRAC	2 cut(s)	1187, 1381
HindIII	AAGCTT	1 cut(s)	2046
HinfI	GANTC	7 cut(s)	145, 548, 1076, 1172, 1619, 1625, 1736
HpaII	CCGG	3 cut(s)	226, 941, 1898
HphI	GGTGA	4 cut(s)	271, 1226, 1259, 1550
Hpy166II	GTNNAC	5 cut(s)	1187, 1212, 1381, 1586, 1697
Hpy188III	TCNNGA	6 cut(s)	104, 149, 362, 437, 524, 2112
Hpy8I	GTNNAC	5 cut(s)	1187, 1212, 1381, 1586, 1697
Hpy99I	CGWCG	2 cut(s)	82, 690
HpyAV	CCTTC	9 cut(s)	226, 292, 359, 1322, 1522, 1559, 1658, 2116, 2286
HpyCH4III	ACNGT	5 cut(s)	580, 1357, 1583, 1694, 1835
HpyCH4IV	ACGT	5 cut(s)	206, 447, 1391, 1648, 1866
HpyF10VI	GCNNNNNNNGC	7 cut(s)	253, 506, 787, 1043, 1052, 1136, 2339
HpyF3I	CTNAG	5 cut(s)	270, 402, 819, 1879, 2292
HpySE526I	ACGT	5 cut(s)	206, 447, 1391, 1648, 1866
Hsp92I	GRCGYC	1 cut(s)	2255
HspAI	GCGC	2 cut(s)	1483, 2255
KasI	GGCGCC	1 cut(s)	2254
Ksp22I	TGATCA	1 cut(s)	93
Kzo9I	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
LmnI	GCTCC	3 cut(s)	6, 317, 2189
Lsp1109I	GCAGC	4 cut(s)	49, 584, 1212, 2326
MaeI	CTAG	7 cut(s)	188, 299, 348, 791, 894, 963, 2009
MaeII	ACGT	5 cut(s)	206, 447, 1391, 1648, 1866
MaeIII	GTNAC	8 cut(s)	384, 415, 604, 706, 1232, 1321, 1495, 1829
MalI	GATC	8 cut(s)	95, 489, 891, 1415, 1576, 1687, 2224, 2306
MboI	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
MfeI	CAATTG	1 cut(s)	248
MflI	RGATCY	3 cut(s)	487, 889, 1413
MhlI	GDGCHC	2 cut(s)	11, 60
MlsI	TGGCCA	2 cut(s)	832, 2333
MluNI	TGGCCA	2 cut(s)	832, 2333
Mly113I	GGCGCC	1 cut(s)	2255
MlyI	GAGTC	7 cut(s)	154, 542, 1085, 1181, 1619, 1628, 1730
MmeI	TCCRAC	2 cut(s)	101, 322
Mox20I	TGGCCA	2 cut(s)	832, 2333
Mph1103I	ATGCAT	1 cut(s)	668
MroXI	GAANNNNTTC	1 cut(s)	2275
MscI	TGGCCA	2 cut(s)	832, 2333
MseI	TTAA	5 cut(s)	17, 213, 465, 659, 1368
MslI	CAYNNNNRTG	2 cut(s)	661, 1245
Msp20I	TGGCCA	2 cut(s)	832, 2333
MspA1I	CMGCKG	1 cut(s)	40
MspI	CCGG	3 cut(s)	226, 941, 1898
MspR9I	CCNGG	5 cut(s)	315, 1245, 1404, 1464, 1898
MunI	CAATTG	1 cut(s)	248
Mva1269I	GAATGC	3 cut(s)	568, 778, 1378
MvaI	CCWGG	4 cut(s)	315, 1245, 1404, 1464
MwoI	GCNNNNNNNGC	7 cut(s)	253, 506, 787, 1043, 1052, 1136, 2339
NarI	GGCGCC	1 cut(s)	2255
NciI	CCSGG	1 cut(s)	1898
NdeI	CATATG	2 cut(s)	755, 1216
NdeII	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
NlaIV	GGNNCC	4 cut(s)	8, 891, 2256, 2272
NmuCI	GTSAC	4 cut(s)	415, 1232, 1321, 1829
NsiI	ATGCAT	1 cut(s)	668
NspI	RCATGY	3 cut(s)	356, 1225, 1748
PaeI	GCATGC	1 cut(s)	1748
PaeR7I	CTCGAG	2 cut(s)	102, 360
PctI	GAATGC	3 cut(s)	568, 778, 1378
PdmI	GAANNNNTTC	1 cut(s)	2275
PkrI	GCNGC	4 cut(s)	39, 599, 1227, 2341
PleI	GAGTC	7 cut(s)	153, 542, 1084, 1180, 1619, 1627, 1730
PluTI	GGCGCC	1 cut(s)	2258
PpsI	GAGTC	7 cut(s)	153, 542, 1084, 1180, 1619, 1627, 1730
PpuMI	RGGWCCY	1 cut(s)	2104
Psp5II	RGGWCCY	1 cut(s)	2104
Psp6I	CCWGG	4 cut(s)	313, 1243, 1402, 1462
PspFI	CCCAGC	1 cut(s)	1164
PspGI	CCWGG	4 cut(s)	313, 1243, 1402, 1462
PspN4I	GGNNCC	4 cut(s)	8, 891, 2256, 2272
PspPI	GGNCC	6 cut(s)	74, 311, 344, 1294, 2027, 2104
PspPPI	RGGWCCY	1 cut(s)	2104
PsrI	GAACNNNNNNTAC	2 cut(s)	950, 982
PstI	CTGCAG	3 cut(s)	602, 1230, 1769
PstNI	CAGNNNCTG	1 cut(s)	1325
PsuI	RGATCY	3 cut(s)	487, 889, 1413
RsaI	GTAC	4 cut(s)	140, 985, 1211, 2007
RsaNI	GTAC	4 cut(s)	139, 984, 1210, 2006
RseI	CAYNNNNRTG	2 cut(s)	661, 1245
Rsr2I	CGGWCCG	1 cut(s)	74
RsrII	CGGWCCG	1 cut(s)	74
SaqAI	TTAA	5 cut(s)	17, 213, 465, 659, 1368
SatI	GCNGC	4 cut(s)	38, 598, 1226, 2340
Sau3AI	GATC	8 cut(s)	93, 487, 889, 1413, 1574, 1685, 2222, 2304
Sau96I	GGNCC	6 cut(s)	74, 311, 344, 1294, 2027, 2104
ScaI	AGTACT	1 cut(s)	985
SchI	GAGTC	7 cut(s)	154, 542, 1085, 1181, 1619, 1628, 1730
ScrFI	CCNGG	5 cut(s)	315, 1245, 1404, 1464, 1898
SduI	GDGCHC	2 cut(s)	11, 60
SfcI	CTRYAG	4 cut(s)	557, 598, 1226, 1765
SfoI	GGCGCC	1 cut(s)	2256
Sfr274I	CTCGAG	2 cut(s)	102, 360
SinI	GGWCC	4 cut(s)	74, 344, 2027, 2104
SlaI	CTCGAG	2 cut(s)	102, 360
SmiMI	CAYNNNNRTG	2 cut(s)	661, 1245
SmlI	CTYRAG	6 cut(s)	102, 360, 1545, 1627, 1738, 2234
SmoI	CTYRAG	6 cut(s)	102, 360, 1545, 1627, 1738, 2234
SphI	GCATGC	1 cut(s)	1748
SsiI	CCGC	8 cut(s)	40, 72, 649, 978, 1238, 1600, 1711, 2282
SspDI	GGCGCC	1 cut(s)	2254
SspMI	CTAG	7 cut(s)	188, 299, 348, 791, 894, 963, 2009
StyD4I	CCNGG	5 cut(s)	313, 1243, 1402, 1462, 1896
TaaI	ACNGT	5 cut(s)	580, 1357, 1583, 1694, 1835
TaiI	ACGT	5 cut(s)	209, 450, 1394, 1651, 1869
TaqI	TCGA	5 cut(s)	103, 111, 361, 1474, 1839
TaqII	GACCGA	1 cut(s)	2015
TatI	WGTACW	1 cut(s)	983
Tru1I	TTAA	5 cut(s)	17, 213, 465, 659, 1368
Tru9I	TTAA	5 cut(s)	17, 213, 465, 659, 1368
TscAI	CASTG	9 cut(s)	478, 783, 827, 1132, 1235, 1330, 1588, 1699, 1774
TseFI	GTSAC	4 cut(s)	415, 1232, 1321, 1829
TseI	GCWGC	4 cut(s)	37, 597, 1225, 2339
Tsp45I	GTSAC	4 cut(s)	415, 1232, 1321, 1829
TspGWI	ACGGA	4 cut(s)	95, 1172, 1406, 2283
TspRI	CASTG	9 cut(s)	478, 783, 827, 1132, 1235, 1330, 1588, 1699, 1774
VpaK11BI	GGWCC	4 cut(s)	74, 344, 2027, 2104
XapI	RAATTY	4 cut(s)	461, 615, 1364, 2317
XceI	RCATGY	3 cut(s)	356, 1225, 1748
XhoI	CTCGAG	2 cut(s)	102, 360
XmnI	GAANNNNTTC	1 cut(s)	2275
XspI	CTAG	7 cut(s)	188, 299, 348, 791, 894, 963, 2009
ZrmI	AGTACT	1 cut(s)	985
Zsp2I	ATGCAT	1 cut(s)	668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.