Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005853

Physical Location & Seq

Genomic Coordinates Reverse (-)

35379827 .. 35385248

Gene Structure 5422 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1222200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGGGAGCCCTGCATTAAGCAACCGAAATAACGGGCAGCGGCTAGGCATTACAGAGCCCATTTCTCTGGGCGGGCCGACGGAGTATGATGTGATCAAAACTCGAGAACTCGAAAAGTATTTGCAAGATGCTGGATTGTATGAGAGTCAGGAAGAGGCTATTGTGAAGACGTGGGTTAAAACAATTAGCCGGGCAAAGGGCTTGAATGAGCAACTGGTGCATGAAGCAAATGCTAAGATTTTCACCTTTGGTTCATATCGGCTAGGGGTTCATGGCCCTGGAGCAGATATAGACACACTCTGCGTTGGACCTAGACATGCAACTCGAGAAGGAGATTTCTTTGGTGAGCTTCATAGGATGCTGTCTGAGATGTCTGAAGTGACAGAGTTACATCCTGTTCCTGATGCTCACGTCCCAGTTATGAAATTCAAGTTCAGTGGGGTTTCAATAGATCTTCTTTATGCACAGCTAGCACTTTGGGTTGTTCCTGAAGACTTGGATATATCACAAGATTCAATACTACAGAATGCAGATGAACAGACAGTTCGTAGTCTCAATGGCTGTAGAGTTACAGACCAAATTCTGCGTTTAGTCCCTAACATTCAGAGTTTCCGCACTACATTGAGATGCATGAGGTTATGGGCAAAGCATCGTGGTGTTTATTCAAATGTTACAGGTTTTCTTGGTGGTATAAACTGGGCAATTCTTGTTGCTCGCATATGCCAACTTTACCCGAATGCACTGCCTAATATGCTAGTTTCTCGGTTCTTCAGGGTATATACTCAGTGGCGTTGGCCAAATCCAGTCATGCTTTGTGCTATTGAAGATGGGTTTCTTGGACTTCAGGTTTGGGATCCTAGAAAATATATAAAGGACAAATATCATTTGATGCCTATAATTACTCCGGCTTATCCCAGCATGAACTCTAGCTACAATGTGTCTGCAAGTACTTTGCGTATCATGTTAGAGGAGTTTCAGAGGGGTAATGAAATTTGTGAGGCTATGGAAGCAAATAAGGCTGATTGGGATACCCTTTTTGAGTCTTATGCTTTCTTTGAAGCGTATAAGAATTATTTACAGATAGACATCGGTGCAGAAAATGCTGATGACTTTAGACAATGGAAAGGTTGGGTTGAGTCTCGTCTCCGTCAACTAACCCTGAAGATTGAGAGGTACACAGACATGCTGCAGTGTCACCCACATCCAGGTGATTTTTCAGACAGATCGAGACCTTTTCATAGTTCTTTCTTTATGGGCCTGCAACGCAAACAAGGAGTTCCAGTCACTGAAGGCAAACAGTTTGACATAAGAGGAACTGTCGAGGGATTTAAGAATGCTGTCAACCTTTACTCATTATTGAAGCCTGGAATGGCGATCCGTGTATACCATGTAAAACGCAGGAACATTCCTAATTTTGTGTTTCCTGGTGGTGTTCGACGTTTGCGCCCATCTAAATTTACTTGGGACAGCAGGCGGAGTTCAGAACTGAAGGCTTCTGGCAATGCCCAGGCAGAGAAATCGTGCGAAGGAAATGGATCAGATAGTGGACAGAAGAGAAAGCGGGTGGATGGTAATTTAGAGACTGACTCAAGGGATGCCAAGTCTTTACGTCTCTGCAGTGGGGAAGGTAAAACTGATTTAGATGGATCAGACAGTGGACAGAAGAGAAAGCGGGTGGATGATAATGTAGAAACTGACTCAAGGCATGCCAAGTCTTTACATCTCTGCAGCGAGGAAGTTCGTGAGGCTAGCCCTCCTATCAGCAATGTCAGTTCGCGTTCCACAAAATGTGACAGTATCGATGCAAACAAAAACCACAATAATAACGTGAAATTTAGCATAGGGAAGATGGAAAACCCCGCAGACATTCCTTGTCAGAATGGTCAAACTGAAGTATCATCGAGGTGCAATCCACCAGTCGATAGTCTATCTTCTGCTGTGAATACATCAAGTTCTAAAGAAGAGGCACTAGAGAAGATTATGGTTGGTCCATATGTTTCCCACCAAGCTTTGCCGGAAGTAGATGAGCTTGAAGATGATTTGGAGTACAGACTTCAAGTGAAAGACTTTTCAGGAAATATGAAAGGCAGTCGATTGGAGGCTCTGGAGGAGAGTGTGTCAATTGCAACACCAGTAAATTATAGCAACGGAGCTGGTCCTTCCACCGATTTATATAATGGGGGATTAGAGGAACTTGAGCCTGCTGAACTTATGGCGCCACTCTCAAACGGAACCCCTTCTCTGCCTGTGGCTCAGAGAAAGCCGATCATCAGGTTGAATTTCACTTCATTGGCCAAACCTGCCGGTAAAAGCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

773

Amino Acids

86.48

Weight (kDa)

6.51

Isoelectric Point (pI)

46.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	16 - 198	2.1e-70	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	74 - 152	2.7e-10	Nucleotidyltransferase domain
PAP_central	PF04928	203 - 347	1.4e-68	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	351 - 408	9e-13	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17980 AT1G17980
fragaria_vesca	FvH4_5g16820 FvH4_5g16820 FvH4_5g16820 FvH4_5g16820
malus_domestica	MD06G1222200.v1.1 MD09G1233900.v1.1 MD14G1231300.v1.1 MD17G1263100.v1.1
prunus_persica	Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1
pyrus_communis	pycom06g19770 pycom14g19250 pycom17g26340
rosa_chinensis	RchiOBHm_Chr7g0179251
rosa_laevigata	RLG00000005369
rosa_multiflora	Rmu_sc0006939.1_g000005
rosa_roxburghii	Rroxscaffold_3G00274180
rosa_rugosa	Rorug06G0422700 Rorug06G0422800 Rorug06G0422800
rosa_samantha	Rh7AG022000 Rh7BG021900 Rh7CG023200 Rh7DG022500
rosa_wichuraiana	Rw7G001810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2308
AccB1I	GGYRCC	1 cut(s)	2215
AccI	GTMKAC	1 cut(s)	1383
AccII	CGCG	1 cut(s)	1777
AciI	CCGC	7 cut(s)	40, 72, 613, 1474, 1561, 1672, 1860
AclWI	GGATC	5 cut(s)	848, 861, 1369, 1543, 1654
AcoI	YGGCCR	2 cut(s)	794, 2292
AcsI	RAATTY	6 cut(s)	425, 579, 990, 1454, 1832, 2278
AcuI	CTGAAG	8 cut(s)	396, 510, 754, 827, 1181, 1308, 1508, 1911
AcyI	GRCGYC	1 cut(s)	2216
AfaI	GTAC	3 cut(s)	949, 1175, 2048
AfiI	CCNNNNNNNGG	2 cut(s)	196, 1205
AjiI	CACGTC	2 cut(s)	171, 412
AjnI	CCWGG	5 cut(s)	277, 1204, 1363, 1423, 1506
AluBI	AGCT	7 cut(s)	349, 469, 930, 2009, 2029, 2153, 2313
AluI	AGCT	7 cut(s)	349, 469, 930, 2009, 2029, 2153, 2313
Alw26I	GTCTC	6 cut(s)	557, 1143, 1148, 1222, 1574, 1616
AlwI	GGATC	5 cut(s)	848, 861, 1369, 1543, 1654
AlwNI	CAGNNNCTG	1 cut(s)	1286
Ama87I	CYCGRG	2 cut(s)	102, 324
AoxI	GGCC	5 cut(s)	74, 274, 794, 1255, 2292
ApeKI	GCWGC	3 cut(s)	37, 1186, 1728
ApoI	RAATTY	6 cut(s)	425, 579, 990, 1454, 1832, 2278
ArsI	GACNNNNNNTTYG	2 cut(s)	831, 863
Asp700I	GAANNNNTTC	1 cut(s)	2236
AspLEI	GCGC	2 cut(s)	1446, 2218
AspS9I	GGNCC	6 cut(s)	74, 275, 308, 1255, 1988, 2156
AsuC2I	CCSGG	1 cut(s)	191
AsuHPI	GGTGA	4 cut(s)	235, 356, 1187, 1220
AsuNHI	GCTAGC	2 cut(s)	469, 1748
AvaI	CYCGRG	2 cut(s)	102, 324
AvaII	GGWCC	3 cut(s)	308, 1988, 2156
BaeI	ACNNNNGTAYC	2 cut(s)	1878, 1911
BalI	TGGCCA	2 cut(s)	796, 2294
BamHI	GGATCC	1 cut(s)	853
BanI	GGYRCC	1 cut(s)	2215
BanII	GRGCYC	2 cut(s)	11, 60
BbsI	GAAGAC	2 cut(s)	173, 498
BbvI	GCAGC	3 cut(s)	49, 1173, 1740
BccI	CCATC	5 cut(s)	821, 1456, 1562, 1637, 1843
BcgI	CGANNNNNNTGC	4 cut(s)	724, 758, 1500, 1534
BciT130I	CCWGG	5 cut(s)	279, 1206, 1365, 1425, 1508
BciVI	GTATCC	1 cut(s)	1021
BclI	TGATCA	1 cut(s)	93
BcnI	CCSGG	1 cut(s)	191
BcoDI	GTCTC	6 cut(s)	557, 1143, 1148, 1222, 1574, 1616
BfaI	CTAG	9 cut(s)	44, 263, 312, 470, 755, 858, 927, 1749, 1970
BfmI	CTRYAG	5 cut(s)	521, 562, 1187, 1615, 1726
BfoI	RGCGCY	1 cut(s)	2219
BfuAI	ACCTGC	1 cut(s)	2308
BfuI	GTATCC	1 cut(s)	1021
BglII	AGATCT	1 cut(s)	451
BisI	GCNGC	4 cut(s)	38, 41, 1187, 1729
BlsI	GCNGC	4 cut(s)	39, 42, 1188, 1730
BmcAI	AGTACT	1 cut(s)	949
Bme1390I	CCNGG	6 cut(s)	191, 279, 1206, 1365, 1425, 1508
Bme18I	GGWCC	3 cut(s)	308, 1988, 2156
BmeT110I	CYCGRG	2 cut(s)	102, 324
BmgBI	CACGTC	2 cut(s)	171, 412
BmgT120I	GGNCC	6 cut(s)	74, 275, 308, 1255, 1988, 2156
BmiI	GGNNCC	4 cut(s)	8, 855, 2217, 2233
BmrFI	CCNGG	6 cut(s)	191, 279, 1206, 1365, 1425, 1508
BmrI	ACTGGG	2 cut(s)	410, 706
BmsI	GCATC	8 cut(s)	118, 348, 394, 617, 658, 879, 1585, 1792
BmtI	GCTAGC	2 cut(s)	473, 1752
BmuI	ACTGGG	2 cut(s)	410, 706
BpiI	GAAGAC	2 cut(s)	173, 498
BplI	GAGNNNNNCTC	2 cut(s)	1571, 1603
BpmI	CTGGAG	2 cut(s)	300, 2126
BpuEI	CTTGAG	3 cut(s)	1573, 1684, 2216
BpuMI	CCSGG	1 cut(s)	191
Bsa29I	ATCGAT	1 cut(s)	1800
BsaBI	GATNNNNATC	1 cut(s)	1085
BsaHI	GRCGYC	1 cut(s)	2216
BsaI	GGTCTC	1 cut(s)	1222
BsaJI	CCNNGG	2 cut(s)	277, 1506
BsaXI	ACNNNNNCTCC	4 cut(s)	1266, 1296, 2102, 2132
Bsc4I	CCNNNNNNNGG	2 cut(s)	196, 1205
Bse118I	RCCGGY	1 cut(s)	2303
Bse1I	ACTGG	7 cut(s)	219, 416, 701, 803, 1280, 1916, 2132
Bse3DI	GCAATG	2 cut(s)	1507, 1771
Bse8I	GATNNNNATC	1 cut(s)	1085
BseBI	CCWGG	5 cut(s)	279, 1206, 1365, 1425, 1508
BseCI	ATCGAT	1 cut(s)	1800
BseDI	CCNNGG	2 cut(s)	277, 1506
BseGI	GGATG	6 cut(s)	363, 391, 1201, 1573, 1600, 1684
BseJI	GATNNNNATC	1 cut(s)	1085
BseLI	CCNNNNNNNGG	2 cut(s)	196, 1205
BseMI	GCAATG	2 cut(s)	1507, 1771
BseMII	CTCAG	3 cut(s)	357, 797, 2267
BseNI	ACTGG	7 cut(s)	219, 416, 701, 803, 1280, 1916, 2132
BseRI	GAGGAG	2 cut(s)	983, 2123
BseXI	GCAGC	3 cut(s)	49, 1173, 1740
BseYI	CCCAGC	1 cut(s)	914
BsgI	GTGCAG	1 cut(s)	1113
Bsh1236I	CGCG	1 cut(s)	1777
BshFI	GGCC	5 cut(s)	76, 276, 796, 1257, 2294
BshNI	GGYRCC	1 cut(s)	2215
BshVI	ATCGAT	1 cut(s)	1800
BsiHKCI	CYCGRG	2 cut(s)	102, 324
BsiSI	CCGG	4 cut(s)	190, 905, 2015, 2304
BslFI	GGGAC	3 cut(s)	398, 578, 1478
BslI	CCNNNNNNNGG	2 cut(s)	196, 1205
BsmAI	GTCTC	6 cut(s)	557, 1143, 1148, 1222, 1574, 1616
BsmBI	CGTCTC	2 cut(s)	1148, 1616
BsmFI	GGGAC	3 cut(s)	398, 578, 1478
BsmI	GAATGC	3 cut(s)	532, 742, 1339
BsnI	GGCC	5 cut(s)	76, 276, 796, 1257, 2294
Bso31I	GGTCTC	1 cut(s)	1222
BsoBI	CYCGRG	2 cut(s)	102, 324
Bsp1286I	GDGCHC	2 cut(s)	11, 60
Bsp143I	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
BspACI	CCGC	7 cut(s)	40, 72, 613, 1474, 1561, 1672, 1860
BspANI	GGCC	5 cut(s)	76, 276, 796, 1257, 2294
BspCNI	CTCAG	3 cut(s)	358, 796, 2266
BspDI	ATCGAT	1 cut(s)	1800
BspFNI	CGCG	1 cut(s)	1777
BspLI	GGNNCC	4 cut(s)	8, 855, 2217, 2233
BspMAI	CTGCAG	3 cut(s)	1191, 1619, 1730
BspMI	ACCTGC	1 cut(s)	2308
BspOI	GCTAGC	2 cut(s)	473, 1752
BspPI	GGATC	5 cut(s)	848, 861, 1369, 1543, 1654
BspT107I	GGYRCC	1 cut(s)	2215
BspTNI	GGTCTC	1 cut(s)	1222
BsrDI	GCAATG	2 cut(s)	1507, 1771
BsrFI	RCCGGY	1 cut(s)	2303
BsrI	ACTGG	7 cut(s)	219, 416, 701, 803, 1280, 1916, 2132
BssAI	RCCGGY	1 cut(s)	2303
BssECI	CCNNGG	2 cut(s)	277, 1506
BssMI	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
BssNAI	GTATAC	1 cut(s)	1384
BssNI	GRCGYC	1 cut(s)	2216
Bst1107I	GTATAC	1 cut(s)	1384
Bst2UI	CCWGG	5 cut(s)	279, 1206, 1365, 1425, 1508
Bst4CI	ACNGT	5 cut(s)	544, 1299, 1318, 1655, 1796
Bst6I	CTCTTC	4 cut(s)	147, 1547, 1658, 1956
BstACI	GRCGYC	1 cut(s)	2216
BstAPI	GCANNNNNTGC	2 cut(s)	217, 1100
BstC8I	GCNNGC	8 cut(s)	74, 471, 715, 1259, 1472, 1707, 1750, 2202
BstDEI	CTNAG	4 cut(s)	234, 366, 783, 2253
BstF5I	GGATG	6 cut(s)	363, 391, 1201, 1573, 1600, 1684
BstFNI	CGCG	1 cut(s)	1777
BstH2I	RGCGCY	1 cut(s)	2219
BstHHI	GCGC	2 cut(s)	1446, 2218
BstKTI	GATC	8 cut(s)	96, 454, 856, 1226, 1377, 1538, 1649, 2268
BstMAI	GTCTC	6 cut(s)	557, 1143, 1148, 1222, 1574, 1616
BstMBI	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
BstMWI	GCNNNNNNNGC	8 cut(s)	217, 470, 751, 1007, 1016, 1100, 1263, 2300
BstNI	CCWGG	5 cut(s)	279, 1206, 1365, 1425, 1508
BstNSI	RCATGY	3 cut(s)	320, 1186, 1709
BstSCI	CCNGG	6 cut(s)	189, 277, 1204, 1363, 1423, 1506
BstSFI	CTRYAG	5 cut(s)	521, 562, 1187, 1615, 1726
BstUI	CGCG	1 cut(s)	1777
BstV1I	GCAGC	3 cut(s)	49, 1173, 1740
BstV2I	GAAGAC	2 cut(s)	173, 498
BstX2I	RGATCY	2 cut(s)	451, 853
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstYI	RGATCY	2 cut(s)	451, 853
BstZ17I	GTATAC	1 cut(s)	1384
Bsu15I	ATCGAT	1 cut(s)	1800
BsuI	GTATCC	1 cut(s)	1021
BsuRI	GGCC	5 cut(s)	76, 276, 796, 1257, 2294
BsuTUI	ATCGAT	1 cut(s)	1800
BtrI	CACGTC	2 cut(s)	171, 412
BtsCI	GGATG	6 cut(s)	363, 391, 1201, 1573, 1600, 1684
BtsI	GCAGTG	3 cut(s)	740, 1196, 1624
BtsIMutI	CAGTG	7 cut(s)	442, 740, 791, 1196, 1284, 1624, 1660
BveI	ACCTGC	1 cut(s)	2308
Cac8I	GCNNGC	8 cut(s)	74, 471, 715, 1259, 1472, 1707, 1750, 2202
CaiI	CAGNNNCTG	1 cut(s)	1286
CfoI	GCGC	2 cut(s)	1446, 2218
Cfr10I	RCCGGY	1 cut(s)	2303
Cfr13I	GGNCC	6 cut(s)	74, 275, 308, 1255, 1988, 2156
ClaI	ATCGAT	1 cut(s)	1800
Csp6I	GTAC	3 cut(s)	948, 1174, 2047
CspCI	CAANNNNNGTGG	2 cut(s)	418, 453
CviQI	GTAC	3 cut(s)	948, 1174, 2047
DdeI	CTNAG	4 cut(s)	234, 366, 783, 2253
DinI	GGCGCC	1 cut(s)	2217
DpnI	GATC	8 cut(s)	95, 453, 855, 1225, 1376, 1537, 1648, 2267
DpnII	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
EaeI	YGGCCR	2 cut(s)	794, 2292
Eam1104I	CTCTTC	4 cut(s)	147, 1547, 1658, 1956
EarI	CTCTTC	4 cut(s)	147, 1547, 1658, 1956
EciI	GGCGGA	1 cut(s)	1489
Eco24I	GRGCYC	2 cut(s)	11, 60
Eco31I	GGTCTC	1 cut(s)	1222
Eco47I	GGWCC	3 cut(s)	308, 1988, 2156
Eco57I	CTGAAG	8 cut(s)	396, 510, 754, 827, 1181, 1308, 1508, 1911
Eco88I	CYCGRG	2 cut(s)	102, 324
EcoRII	CCWGG	5 cut(s)	277, 1204, 1363, 1423, 1506
EcoT22I	ATGCAT	1 cut(s)	632
EcoT38I	GRGCYC	2 cut(s)	11, 60
EgeI	GGCGCC	1 cut(s)	2217
EheI	GGCGCC	1 cut(s)	2217
Esp3I	CGTCTC	2 cut(s)	1148, 1616
FaqI	GGGAC	3 cut(s)	398, 578, 1478
FauI	CCCGC	4 cut(s)	65, 1554, 1665, 1867
FauNDI	CATATG	2 cut(s)	719, 1993
FbaI	TGATCA	1 cut(s)	93
FblI	GTMKAC	1 cut(s)	1383
Fnu4HI	GCNGC	4 cut(s)	38, 41, 1187, 1729
FokI	GGATG	6 cut(s)	370, 378, 1188, 1580, 1607, 1691
FriOI	GRGCYC	2 cut(s)	11, 60
Fsp4HI	GCNGC	4 cut(s)	38, 41, 1187, 1729
FspBI	CTAG	9 cut(s)	44, 263, 312, 470, 755, 858, 927, 1749, 1970
GlaI	GCGC	2 cut(s)	1445, 2217
GluI	GCNGC	4 cut(s)	38, 41, 1187, 1729
GsaI	CCCAGC	1 cut(s)	918
GsuI	CTGGAG	2 cut(s)	300, 2126
HaeII	RGCGCY	1 cut(s)	2219
HaeIII	GGCC	5 cut(s)	76, 276, 796, 1257, 2294
HapII	CCGG	4 cut(s)	190, 905, 2015, 2304
HhaI	GCGC	2 cut(s)	1446, 2218
Hin1I	GRCGYC	1 cut(s)	2216
Hin6I	GCGC	2 cut(s)	1444, 2216
HinP1I	GCGC	2 cut(s)	1444, 2216
HincII	GTYRAC	2 cut(s)	1151, 1342
HindII	GTYRAC	2 cut(s)	1151, 1342
HindIII	AAGCTT	1 cut(s)	2007
HinfI	GANTC	6 cut(s)	145, 512, 1040, 1136, 1586, 1697
HpaII	CCGG	4 cut(s)	190, 905, 2015, 2304
HphI	GGTGA	4 cut(s)	235, 356, 1187, 1220
Hpy166II	GTNNAC	6 cut(s)	1151, 1176, 1342, 1384, 1547, 1658
Hpy188III	TCNNGA	9 cut(s)	104, 149, 326, 401, 488, 1227, 1742, 2073, 2105
Hpy8I	GTNNAC	6 cut(s)	1151, 1176, 1342, 1384, 1547, 1658
Hpy99I	CGWCG	2 cut(s)	82, 1440
HpyAV	CCTTC	7 cut(s)	323, 1283, 1483, 1520, 1619, 2169, 2247
HpyCH4III	ACNGT	5 cut(s)	544, 1299, 1318, 1655, 1796
HpyCH4IV	ACGT	5 cut(s)	170, 411, 1438, 1609, 1827
HpyF10VI	GCNNNNNNNGC	8 cut(s)	217, 470, 751, 1007, 1016, 1100, 1263, 2300
HpyF3I	CTNAG	4 cut(s)	234, 366, 783, 2253
HpySE526I	ACGT	5 cut(s)	170, 411, 1438, 1609, 1827
Hsp92I	GRCGYC	1 cut(s)	2216
HspAI	GCGC	2 cut(s)	1444, 2216
KasI	GGCGCC	1 cut(s)	2215
Ksp22I	TGATCA	1 cut(s)	93
Kzo9I	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
LmnI	GCTCC	3 cut(s)	6, 281, 2150
Lsp1109I	GCAGC	3 cut(s)	49, 1173, 1740
LweI	GCATC	8 cut(s)	118, 348, 394, 617, 658, 879, 1585, 1792
MaeI	CTAG	9 cut(s)	44, 263, 312, 470, 755, 858, 927, 1749, 1970
MaeII	ACGT	5 cut(s)	170, 411, 1438, 1609, 1827
MaeIII	GTNAC	7 cut(s)	379, 387, 568, 670, 1193, 1282, 1790
MalI	GATC	8 cut(s)	95, 453, 855, 1225, 1376, 1537, 1648, 2267
MboI	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
MfeI	CAATTG	1 cut(s)	2121
MflI	RGATCY	2 cut(s)	451, 853
MhlI	GDGCHC	2 cut(s)	11, 60
MlsI	TGGCCA	2 cut(s)	796, 2294
MluNI	TGGCCA	2 cut(s)	796, 2294
Mly113I	GGCGCC	1 cut(s)	2216
MlyI	GAGTC	5 cut(s)	154, 1049, 1145, 1580, 1691
MmeI	TCCRAC	1 cut(s)	286
Mox20I	TGGCCA	2 cut(s)	796, 2294
Mph1103I	ATGCAT	1 cut(s)	632
MroXI	GAANNNNTTC	1 cut(s)	2236
MscI	TGGCCA	2 cut(s)	796, 2294
MseI	TTAA	3 cut(s)	17, 177, 1329
MslI	CAYNNNNRTG	5 cut(s)	625, 654, 1181, 1206, 1903
Msp20I	TGGCCA	2 cut(s)	796, 2294
MspA1I	CMGCKG	1 cut(s)	40
MspI	CCGG	4 cut(s)	190, 905, 2015, 2304
MspR9I	CCNGG	6 cut(s)	191, 279, 1206, 1365, 1425, 1508
MunI	CAATTG	1 cut(s)	2121
Mva1269I	GAATGC	3 cut(s)	532, 742, 1339
MvaI	CCWGG	5 cut(s)	279, 1206, 1365, 1425, 1508
MvnI	CGCG	1 cut(s)	1777
MwoI	GCNNNNNNNGC	8 cut(s)	217, 470, 751, 1007, 1016, 1100, 1263, 2300
NarI	GGCGCC	1 cut(s)	2216
NciI	CCSGG	1 cut(s)	191
NdeI	CATATG	2 cut(s)	719, 1993
NdeII	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
NheI	GCTAGC	2 cut(s)	469, 1748
NlaIV	GGNNCC	4 cut(s)	8, 855, 2217, 2233
NmuCI	GTSAC	4 cut(s)	379, 1193, 1282, 1790
NsiI	ATGCAT	1 cut(s)	632
NspI	RCATGY	3 cut(s)	320, 1186, 1709
PaeI	GCATGC	1 cut(s)	1709
PaeR7I	CTCGAG	2 cut(s)	102, 324
PctI	GAATGC	3 cut(s)	532, 742, 1339
PdmI	GAANNNNTTC	1 cut(s)	2236
PfeI	GAWTC	1 cut(s)	512
PkrI	GCNGC	4 cut(s)	39, 42, 1188, 1730
PleI	GAGTC	5 cut(s)	153, 1048, 1144, 1580, 1691
PluTI	GGCGCC	1 cut(s)	2219
PpsI	GAGTC	5 cut(s)	153, 1048, 1144, 1580, 1691
Psp6I	CCWGG	5 cut(s)	277, 1204, 1363, 1423, 1506
PspFI	CCCAGC	1 cut(s)	914
PspGI	CCWGG	5 cut(s)	277, 1204, 1363, 1423, 1506
PspN4I	GGNNCC	4 cut(s)	8, 855, 2217, 2233
PspPI	GGNCC	6 cut(s)	74, 275, 308, 1255, 1988, 2156
PsrI	GAACNNNNNNTAC	2 cut(s)	914, 946
PstI	CTGCAG	3 cut(s)	1191, 1619, 1730
PstNI	CAGNNNCTG	1 cut(s)	1286
PsuI	RGATCY	2 cut(s)	451, 853
RsaI	GTAC	3 cut(s)	949, 1175, 2048
RsaNI	GTAC	3 cut(s)	948, 1174, 2047
RseI	CAYNNNNRTG	5 cut(s)	625, 654, 1181, 1206, 1903
SaqAI	TTAA	3 cut(s)	17, 177, 1329
SatI	GCNGC	4 cut(s)	38, 41, 1187, 1729
Sau3AI	GATC	8 cut(s)	93, 451, 853, 1223, 1374, 1535, 1646, 2265
Sau96I	GGNCC	6 cut(s)	74, 275, 308, 1255, 1988, 2156
ScaI	AGTACT	1 cut(s)	949
SchI	GAGTC	5 cut(s)	154, 1049, 1145, 1580, 1691
ScrFI	CCNGG	6 cut(s)	191, 279, 1206, 1365, 1425, 1508
SduI	GDGCHC	2 cut(s)	11, 60
SfaNI	GCATC	8 cut(s)	118, 348, 394, 617, 658, 879, 1585, 1792
SfcI	CTRYAG	5 cut(s)	521, 562, 1187, 1615, 1726
SfoI	GGCGCC	1 cut(s)	2217
Sfr274I	CTCGAG	2 cut(s)	102, 324
SinI	GGWCC	3 cut(s)	308, 1988, 2156
SlaI	CTCGAG	2 cut(s)	102, 324
SmiMI	CAYNNNNRTG	5 cut(s)	625, 654, 1181, 1206, 1903
SmlI	CTYRAG	5 cut(s)	102, 324, 1588, 1699, 2195
SmoI	CTYRAG	5 cut(s)	102, 324, 1588, 1699, 2195
SphI	GCATGC	1 cut(s)	1709
SsiI	CCGC	7 cut(s)	40, 72, 613, 1474, 1561, 1672, 1860
SspDI	GGCGCC	1 cut(s)	2215
SspMI	CTAG	9 cut(s)	44, 263, 312, 470, 755, 858, 927, 1749, 1970
StyD4I	CCNGG	6 cut(s)	189, 277, 1204, 1363, 1423, 1506
TaaI	ACNGT	5 cut(s)	544, 1299, 1318, 1655, 1796
TaiI	ACGT	5 cut(s)	173, 414, 1441, 1612, 1830
TatI	WGTACW	2 cut(s)	947, 2046
TauI	GCSGC	1 cut(s)	43
TfiI	GAWTC	1 cut(s)	512
Tru1I	TTAA	3 cut(s)	17, 177, 1329
Tru9I	TTAA	3 cut(s)	17, 177, 1329
TscAI	CASTG	7 cut(s)	442, 747, 791, 1196, 1291, 1624, 1660
TseFI	GTSAC	4 cut(s)	379, 1193, 1282, 1790
TseI	GCWGC	3 cut(s)	37, 1186, 1728
Tsp45I	GTSAC	4 cut(s)	379, 1193, 1282, 1790
TspGWI	ACGGA	5 cut(s)	95, 1136, 1367, 2163, 2244
TspRI	CASTG	7 cut(s)	442, 747, 791, 1196, 1291, 1624, 1660
VpaK11BI	GGWCC	3 cut(s)	308, 1988, 2156
XapI	RAATTY	6 cut(s)	425, 579, 990, 1454, 1832, 2278
XceI	RCATGY	3 cut(s)	320, 1186, 1709
XhoI	CTCGAG	2 cut(s)	102, 324
XmiI	GTMKAC	1 cut(s)	1383
XmnI	GAANNNNTTC	1 cut(s)	2236
XspI	CTAG	9 cut(s)	44, 263, 312, 470, 755, 858, 927, 1749, 1970
ZrmI	AGTACT	1 cut(s)	949
Zsp2I	ATGCAT	1 cut(s)	632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.