Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0005853

Physical Location & Seq

Genomic Coordinates Reverse (-)

65849337 .. 65854828

Gene Structure 5492 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00274180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2253 bp

ATGGTAATGGGGAGCCCCGGATTAAGCAACCGGAATAATGGCCAGCAGCGGTTAGGCATTACTGAGCCGATTTCGTTGGGCGGTCCGTCGGAGGAGGATGTGATCAAAAGCCGTGAGCTTGAGAAGTATTTGCATGATGCTGGATTGTATGAGAGTCAGGAAGAGGCTGTAAGTAGGGAAGAGGTGCTTGGAAGGCTCGATCAGATTGTGAAGATATGGGTTAAGACCATTAGCCGGGCGAAGGGTTTGAACGATGAACTGGTGCATCAAGCAAATGCTAAGATCTTCACGTTTGGTTCATATAGGCTAGGGGTTCATGGCCCTGGTGCAGATATAGACACACTCTGTGTTGGACCCAGACATGCAACTCGAGAAGAAGATTTCTTTGGTGAGCTATACAGGATGCTATCTGAAATGTCTGAAGTAACGGAGTTGCTTCCAGTTCCTGATGCCCATGTTCCCGTTATGAGGTTCAAGTTTAACGGAGTATCAATTGATCTTCTTTATGCAAAACTATCTCTTTATATTATTCCTGAGGACTTAGATGTATCACAAGATGCAATACTACATAGTGCAGATGAACAGACAGTTCGTAGTCTCAATGGCTGTAGAGTTACAGACCAAATTCTGCGTCTAGTCCCAAGCATTCAGAATTTCCGCACAACACTAAGATGCATGAAGTTATGGGCAAAGCGTCGTGGTGTTTATTCAAATGTTGCAGGATTTCTTGGTGGTATAAACTGGGCACTGCTTGTTGCTCGCATATGCCAACTTTACCCGAATGCATTGCCTAATATGCTAGTATCTCGGTTCTTCAGGGTCTATACTCAGTGGCGTTGGCCAAATCCAGTAATGCTCTGTGCTATTGAAGAAGGGTCTCTAGGACTTCCGGTTTGGGATCCCAGAAGAAATCCAAGGGACAGATATCATTTGATGCCTATAATTACTCCAGCTTATCCTTGCATGAACTCTAGCTACAATGTGTCCTCAAGTACTCTGCGTATCATGTTAGAGGAGTTTCAGAGGGGAAATGAAATTTGTGAGGCTATGGAAGCAAATAAGGCTGATTGGGATACCCTTTTTGAGTCGTATGCTTTCTTTGAAGCATATAAGAATTATTTACAGATAGACATCAGTGCAGCAAATGCTGATGGCTTTAGAAAATGGAAAGGCTGGGTTGAGTCCCGTCTCCGTCAACTAACATTGAAGATTGAGAGGAAAACTTATGAAATGCTGCAGTGTCACCCACACCCAGGTGATTTTCCAGATAAATCCAGACCTTTTCATAGCTCATACTTCATGGGCCTACAACGAAAACAAGGAGTTCCAGTCAAGGAAGGTGAGCACTTTGATTTAAGTAAAGCTGTTGATGACTTCAAGCTTGCTGTCTACCAGTATACATCAAGGGAAATTGGAATGGAGATCCGTGTATCCCATATAAAACGCAGGGAGATCCCTAATTTTGTATTTCCTGGAGGCGTTCGGCCTTCGCGCCCATCTAAAGTAAGTTGGGTTAGAAAGCGACGTTCAGAACTGGAGGTTTCTGGCAGCACTCAACCAGATAGGTCAGGTGAAGATAGAACTGTTTCAGATGGAGCAGATAGTGGTAGAAAGAGAAAATTGGAGAATGATAATGTAGAGACTGGCCATGCCAAGTCTATGCGTCCCTGCGTTGGGGAAGTTTGTGAGGCTAGCCCTCCTTTTAGTAATGTCAGCCCATCCTCTGCAAAATATGAGAATAATGTAGAAGTTAGCACAGGAAAGATAGAAAGCCCAGCACAGATTCCTTATCAGAATGGTCAGGCTGAAGTATGGTCAAGGTGCAATCCATTAATCAACAGTCTATCTGTTGCTGGTGATGCATCAAGTTCTAAAGAAGCAGAGAAACTGGCCTTAGAGAAGATTATGGCTGGTCCATATGTTTCACATCAAGCTTTGCCAGAAGAACTGGATGAGCTCGAAGGTGATTCTGAAGACAGGCATCAAGTCAAAGACTTTTCTGGGAAAGACAGTCGCATCCAGCCTTCAATCGAGAAATCTAACAATGGAGCCGAAACTTCCACTGGTTCATATAATGGAGGCTTAGAGGAACTTGAGTTGTTTCCTTCTTTCCATGTATGTTTTGATCGCCCGAAGCCTGCTGAACTTATGGCACCATCCTTGAATGGAACCCCCGAACCTCTGGCACAGAGGAAGCCAATCATCAGGCTGAACTTCACTTCCTTGCCCAATGCTTCTGGAAAGAGCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004652 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005847 GO:0005849 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008285 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0022414 GO:0031123 GO:0031124 GO:0031347 GO:0031348 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045824 GO:0046483 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048444 GO:0048446 GO:0048449 GO:0048451 GO:0048465 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050776 GO:0050777 GO:0050789 GO:0050794 GO:0061458 GO:0065007 GO:0070013 GO:0070566 GO:0071704 GO:0080134 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901360 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

NTP_transf_2 PAP_RNA-bind PAP_central

Protein Analysis

750

Amino Acids

84.39

Weight (kDa)

6.22

Isoelectric Point (pI)

45.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP_NTPase	PF20750	19 - 213	2.4e-77	Poly(A) polymerase nucleotidyltransferase domain
NTP_transf_2	PF01909	88 - 168	2.4e-10	Nucleotidyltransferase domain
PAP_central	PF04928	218 - 362	2.8e-70	Poly(A) polymerase central domain
PAP_RNA-bind	PF04926	366 - 424	4.5e-12	Poly(A) polymerase predicted RNA binding domain
PAP_RNA-bind	PF04926	425 - 497	5.5e-06	Poly(A) polymerase predicted RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17980 AT1G17980
fragaria_vesca	FvH4_5g16820 FvH4_5g16820 FvH4_5g16820 FvH4_5g16820
malus_domestica	MD06G1222200.v1.1 MD09G1233900.v1.1 MD14G1231300.v1.1 MD17G1263100.v1.1
prunus_persica	Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1 Prupe.5G226600_v2.0.a1
pyrus_communis	pycom06g19770 pycom14g19250 pycom17g26340
rosa_chinensis	RchiOBHm_Chr7g0179251
rosa_laevigata	RLG00000005369
rosa_multiflora	Rmu_sc0006939.1_g000005
rosa_roxburghii	Rroxscaffold_3G00274180
rosa_rugosa	Rorug06G0422700 Rorug06G0422800 Rorug06G0422800
rosa_samantha	Rh7AG022000 Rh7BG021900 Rh7CG023200 Rh7DG022500
rosa_wichuraiana	Rw7G001810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2152
AccI	GTMKAC	2 cut(s)	1389, 1397
AccII	CGCG	1 cut(s)	1492
AciI	CCGC	3 cut(s)	49, 81, 658
AclWI	GGATC	4 cut(s)	893, 906, 1417, 1447
AcoI	YGGCCR	3 cut(s)	40, 839, 1645
AcsI	RAATTY	3 cut(s)	624, 652, 1035
AcuI	CTGAAG	4 cut(s)	441, 799, 1827, 1992
AdeI	CACNNNGTG	1 cut(s)	347
AfaI	GTAC	1 cut(s)	994
AfiI	CCNNNNNNNGG	6 cut(s)	234, 241, 468, 1253, 1673, 1674
AgsI	TTSAA	9 cut(s)	250, 475, 711, 869, 1103, 1207, 1378, 2028, 2164
AjnI	CCWGG	3 cut(s)	322, 1252, 1471
AjuI	GAANNNNNNNTTGG	4 cut(s)	171, 203, 369, 401
AleI	CACNNNNGTG	1 cut(s)	1254
AloI	GAACNNNNNNTCC	2 cut(s)	2204, 2236
Alw21I	GWGCWC	3 cut(s)	1347, 1959, 2249
Alw26I	GTCTC	4 cut(s)	602, 882, 1193, 1634
AlwI	GGATC	4 cut(s)	893, 906, 1417, 1447
AlwNI	CAGNNNCTG	1 cut(s)	446
Ama87I	CYCGRG	1 cut(s)	369
AoxI	GGCC	7 cut(s)	40, 319, 839, 1303, 1484, 1645, 1890
ApeKI	GCWGC	4 cut(s)	46, 1139, 1234, 1548
ApoI	RAATTY	3 cut(s)	624, 652, 1035
ArsI	GACNNNNNNTTYG	4 cut(s)	616, 648, 876, 908
AseI	ATTAAT	1 cut(s)	1832
Asp700I	GAANNNNTTC	1 cut(s)	1585
AspLEI	GCGC	1 cut(s)	1494
AspS9I	GGNCC	5 cut(s)	83, 320, 353, 1303, 1913
AsuC2I	CCSGG	2 cut(s)	18, 236
AsuHPI	GGTGA	7 cut(s)	401, 1235, 1268, 1352, 1583, 1868, 1976
AsuNHI	GCTAGC	1 cut(s)	1691
AvaI	CYCGRG	1 cut(s)	369
AvaII	GGWCC	3 cut(s)	83, 353, 1913
AxyI	CCTNAGG	1 cut(s)	534
BaeGI	GKGCMC	1 cut(s)	748
BalI	TGGCCA	3 cut(s)	42, 841, 1647
BamHI	GGATCC	1 cut(s)	898
BanI	GGYRCC	1 cut(s)	2152
BanII	GRGCYC	3 cut(s)	17, 1959, 2249
BbsI	GAAGAC	1 cut(s)	1980
Bbv12I	GWGCWC	3 cut(s)	1347, 1959, 2249
BbvI	GCAGC	4 cut(s)	58, 1151, 1221, 1560
BccI	CCATC	5 cut(s)	1145, 1504, 1586, 1726, 2164
BceAI	ACGGC	1 cut(s)	96
BcgI	CGANNNNNNTGC	2 cut(s)	769, 803
BciT130I	CCWGG	3 cut(s)	324, 1254, 1473
BciVI	GTATCC	2 cut(s)	1066, 1441
BclI	TGATCA	1 cut(s)	102
BcnI	CCSGG	2 cut(s)	18, 236
BcoDI	GTCTC	4 cut(s)	602, 882, 1193, 1634
BfaI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1692
BfmI	CTRYAG	2 cut(s)	607, 1235
BfuI	GTATCC	2 cut(s)	1066, 1441
BglII	AGATCT	1 cut(s)	282
BisI	GCNGC	4 cut(s)	47, 1140, 1235, 1549
BlsI	GCNGC	4 cut(s)	48, 1141, 1236, 1550
BmcAI	AGTACT	1 cut(s)	994
Bme1390I	CCNGG	5 cut(s)	18, 236, 324, 1254, 1473
Bme18I	GGWCC	3 cut(s)	83, 353, 1913
BmeT110I	CYCGRG	1 cut(s)	369
BmgT120I	GGNCC	5 cut(s)	83, 320, 353, 1303, 1913
BmiI	GGNNCC	6 cut(s)	14, 355, 900, 2050, 2154, 2170
BmrFI	CCNGG	5 cut(s)	18, 236, 324, 1254, 1473
BmrI	ACTGGG	1 cut(s)	751
BmtI	GCTAGC	1 cut(s)	1695
BmuI	ACTGGG	1 cut(s)	751
BpiI	GAAGAC	1 cut(s)	1980
BpmI	CTGGAG	3 cut(s)	933, 1494, 1556
BpuEI	CTTGAG	3 cut(s)	140, 973, 2114
BpuMI	CCSGG	2 cut(s)	18, 236
BsaBI	GATNNNNATC	1 cut(s)	1130
BsaI	GGTCTC	1 cut(s)	882
BsaJI	CCNNGG	4 cut(s)	16, 322, 914, 1252
BsaWI	WCCGGW	2 cut(s)	30, 889
BsaXI	ACNNNNNCTCC	2 cut(s)	1314, 1344
Bsc4I	CCNNNNNNNGG	6 cut(s)	234, 241, 468, 1253, 1673, 1674
Bse21I	CCTNAGG	1 cut(s)	534
Bse3DI	GCAATG	1 cut(s)	785
Bse8I	GATNNNNATC	1 cut(s)	1130
BseBI	CCWGG	3 cut(s)	324, 1254, 1473
BseDI	CCNNGG	4 cut(s)	16, 322, 914, 1252
BseGI	GGATG	6 cut(s)	103, 408, 1718, 1957, 2016, 2156
BseJI	GATNNNNATC	1 cut(s)	1130
BseLI	CCNNNNNNNGG	6 cut(s)	234, 241, 468, 1253, 1673, 1674
BseMI	GCAATG	1 cut(s)	785
BseMII	CTCAG	3 cut(s)	54, 525, 842
BseRI	GAGGAG	2 cut(s)	107, 1028
BseSI	GKGCMC	1 cut(s)	748
BseXI	GCAGC	4 cut(s)	58, 1151, 1221, 1560
BseYI	CCCAGC	2 cut(s)	1173, 1774
BsgI	GTGCAG	3 cut(s)	348, 594, 1158
Bsh1236I	CGCG	1 cut(s)	1492
BshFI	GGCC	7 cut(s)	42, 321, 841, 1305, 1486, 1647, 1892
BshNI	GGYRCC	1 cut(s)	2152
BsiHKAI	GWGCWC	3 cut(s)	1347, 1959, 2249
BsiHKCI	CYCGRG	1 cut(s)	369
BsiSI	CCGG	4 cut(s)	18, 31, 235, 890
BslFI	GGGAC	4 cut(s)	623, 932, 1168, 1650
BslI	CCNNNNNNNGG	6 cut(s)	234, 241, 468, 1253, 1673, 1674
BsmAI	GTCTC	4 cut(s)	602, 882, 1193, 1634
BsmBI	CGTCTC	1 cut(s)	1193
BsmFI	GGGAC	4 cut(s)	623, 932, 1168, 1650
BsmI	GAATGC	2 cut(s)	645, 787
BsnI	GGCC	7 cut(s)	42, 321, 841, 1305, 1486, 1647, 1892
Bso31I	GGTCTC	1 cut(s)	882
BsoBI	CYCGRG	1 cut(s)	369
Bsp1286I	GDGCHC	5 cut(s)	17, 748, 1347, 1959, 2249
Bsp143I	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
BspACI	CCGC	3 cut(s)	49, 81, 658
BspANI	GGCC	7 cut(s)	42, 321, 841, 1305, 1486, 1647, 1892
BspCNI	CTCAG	3 cut(s)	55, 526, 841
BspFNI	CGCG	1 cut(s)	1492
BspLI	GGNNCC	6 cut(s)	14, 355, 900, 2050, 2154, 2170
BspMAI	CTGCAG	1 cut(s)	1239
BspOI	GCTAGC	1 cut(s)	1695
BspPI	GGATC	4 cut(s)	893, 906, 1417, 1447
BspT107I	GGYRCC	1 cut(s)	2152
BspTNI	GGTCTC	1 cut(s)	882
BsrDI	GCAATG	1 cut(s)	785
BssECI	CCNNGG	4 cut(s)	16, 322, 914, 1252
BssMI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
BssNAI	GTATAC	1 cut(s)	1398
BssT1I	CCWWGG	1 cut(s)	914
Bst1107I	GTATAC	1 cut(s)	1398
Bst2UI	CCWGG	3 cut(s)	324, 1254, 1473
Bst4CI	ACNGT	4 cut(s)	589, 1585, 1841, 2012
Bst6I	CTCTTC	2 cut(s)	156, 174
BstAPI	GCANNNNNTGC	1 cut(s)	1145
BstC8I	GCNNGC	5 cut(s)	44, 760, 1383, 1693, 2139
BstDEI	CTNAG	8 cut(s)	63, 279, 534, 541, 668, 828, 1894, 2083
BstF5I	GGATG	6 cut(s)	103, 408, 1718, 1957, 2016, 2156
BstFNI	CGCG	1 cut(s)	1492
BstHHI	GCGC	1 cut(s)	1494
BstKTI	GATC	8 cut(s)	105, 202, 285, 499, 901, 1425, 1455, 2128
BstMAI	GTCTC	4 cut(s)	602, 882, 1193, 1634
BstMBI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
BstMWI	GCNNNNNNNGC	6 cut(s)	193, 796, 1052, 1061, 1145, 1859
BstNI	CCWGG	3 cut(s)	324, 1254, 1473
BstNSI	RCATGY	1 cut(s)	365
BstSCI	CCNGG	5 cut(s)	16, 234, 322, 1252, 1471
BstSFI	CTRYAG	2 cut(s)	607, 1235
BstSLI	GKGCMC	1 cut(s)	748
BstUI	CGCG	1 cut(s)	1492
BstV1I	GCAGC	4 cut(s)	58, 1151, 1221, 1560
BstV2I	GAAGAC	1 cut(s)	1980
BstX2I	RGATCY	4 cut(s)	282, 898, 1422, 1452
BstYI	RGATCY	4 cut(s)	282, 898, 1422, 1452
BstZ17I	GTATAC	1 cut(s)	1398
Bsu36I	CCTNAGG	1 cut(s)	534
BsuI	GTATCC	2 cut(s)	1066, 1441
BsuRI	GGCC	7 cut(s)	42, 321, 841, 1305, 1486, 1647, 1892
BtsCI	GGATG	6 cut(s)	103, 408, 1718, 1957, 2016, 2156
BtsI	GCAGTG	2 cut(s)	746, 1244
BtsIMutI	CAGTG	5 cut(s)	746, 836, 1141, 1244, 2061
Cac8I	GCNNGC	5 cut(s)	44, 760, 1383, 1693, 2139
CaiI	CAGNNNCTG	1 cut(s)	446
CfoI	GCGC	1 cut(s)	1494
Cfr13I	GGNCC	5 cut(s)	83, 320, 353, 1303, 1913
CpoI	CGGWCCG	1 cut(s)	83
CseI	GACGC	3 cut(s)	620, 683, 1652
Csp6I	GTAC	1 cut(s)	993
CspI	CGGWCCG	1 cut(s)	83
CviQI	GTAC	1 cut(s)	993
DdeI	CTNAG	8 cut(s)	63, 279, 534, 541, 668, 828, 1894, 2083
DpnI	GATC	8 cut(s)	104, 201, 284, 498, 900, 1424, 1454, 2127
DpnII	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
DraIII	CACNNNGTG	1 cut(s)	347
EaeI	YGGCCR	3 cut(s)	40, 839, 1645
Eam1104I	CTCTTC	2 cut(s)	156, 174
EarI	CTCTTC	2 cut(s)	156, 174
Ecl136II	GAGCTC	2 cut(s)	1957, 2247
Eco130I	CCWWGG	1 cut(s)	914
Eco24I	GRGCYC	3 cut(s)	17, 1959, 2249
Eco31I	GGTCTC	1 cut(s)	882
Eco32I	GATATC	1 cut(s)	926
Eco47I	GGWCC	3 cut(s)	83, 353, 1913
Eco53kI	GAGCTC	2 cut(s)	1957, 2247
Eco57I	CTGAAG	4 cut(s)	441, 799, 1827, 1992
Eco81I	CCTNAGG	1 cut(s)	534
Eco88I	CYCGRG	1 cut(s)	369
EcoICRI	GAGCTC	2 cut(s)	1957, 2247
EcoRII	CCWGG	3 cut(s)	322, 1252, 1471
EcoRV	GATATC	1 cut(s)	926
EcoT14I	CCWWGG	1 cut(s)	914
EcoT22I	ATGCAT	3 cut(s)	677, 787, 1864
EcoT38I	GRGCYC	3 cut(s)	17, 1959, 2249
ErhI	CCWWGG	1 cut(s)	914
Esp3I	CGTCTC	1 cut(s)	1193
FalI	AAGNNNNNCTT	2 cut(s)	171, 203
FaqI	GGGAC	4 cut(s)	623, 932, 1168, 1650
FauNDI	CATATG	2 cut(s)	764, 1918
FbaI	TGATCA	1 cut(s)	102
FblI	GTMKAC	2 cut(s)	1389, 1397
Fnu4HI	GCNGC	4 cut(s)	47, 1140, 1235, 1549
FokI	GGATG	6 cut(s)	110, 415, 1705, 1964, 2003, 2143
FriOI	GRGCYC	3 cut(s)	17, 1959, 2249
Fsp4HI	GCNGC	4 cut(s)	47, 1140, 1235, 1549
FspBI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1692
GlaI	GCGC	1 cut(s)	1493
GluI	GCNGC	4 cut(s)	47, 1140, 1235, 1549
GsaI	CCCAGC	2 cut(s)	1177, 1778
GsuI	CTGGAG	3 cut(s)	933, 1494, 1556
HaeIII	GGCC	7 cut(s)	42, 321, 841, 1305, 1486, 1647, 1892
HapII	CCGG	4 cut(s)	18, 31, 235, 890
HgaI	GACGC	3 cut(s)	620, 683, 1652
HhaI	GCGC	1 cut(s)	1494
Hin6I	GCGC	1 cut(s)	1492
HinP1I	GCGC	1 cut(s)	1492
HincII	GTYRAC	1 cut(s)	1196
HindII	GTYRAC	1 cut(s)	1196
HindIII	AAGCTT	2 cut(s)	1379, 1932
HinfI	GANTC	5 cut(s)	154, 1085, 1181, 1783, 1967
HpaII	CCGG	4 cut(s)	18, 31, 235, 890
HphI	GGTGA	7 cut(s)	401, 1235, 1268, 1352, 1583, 1868, 1976
Hpy166II	GTNNAC	3 cut(s)	1196, 1390, 1398
Hpy188III	TCNNGA	8 cut(s)	158, 371, 446, 533, 1265, 1275, 2032, 2238
Hpy8I	GTNNAC	3 cut(s)	1196, 1390, 1398
Hpy99I	CGWCG	3 cut(s)	91, 699, 1527
HpyAV	CCTTC	8 cut(s)	186, 235, 866, 1331, 1497, 1955, 2034, 2115
HpyCH4III	ACNGT	4 cut(s)	589, 1585, 1841, 2012
HpyCH4IV	ACGT	2 cut(s)	290, 1525
HpyF10VI	GCNNNNNNNGC	6 cut(s)	193, 796, 1052, 1061, 1145, 1859
HpyF3I	CTNAG	8 cut(s)	63, 279, 534, 541, 668, 828, 1894, 2083
HpySE526I	ACGT	2 cut(s)	290, 1525
HspAI	GCGC	1 cut(s)	1492
Ksp22I	TGATCA	1 cut(s)	102
Kzo9I	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
LmnI	GCTCC	3 cut(s)	12, 1595, 2048
Lsp1109I	GCAGC	4 cut(s)	58, 1151, 1221, 1560
MaeI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1692
MaeII	ACGT	2 cut(s)	290, 1525
MaeIII	GTNAC	3 cut(s)	424, 613, 1241
MalI	GATC	8 cut(s)	104, 201, 284, 498, 900, 1424, 1454, 2127
MboI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
MfeI	CAATTG	1 cut(s)	492
MflI	RGATCY	4 cut(s)	282, 898, 1422, 1452
MhlI	GDGCHC	5 cut(s)	17, 748, 1347, 1959, 2249
MlsI	TGGCCA	3 cut(s)	42, 841, 1647
MluCI	AATT	9 cut(s)	492, 624, 652, 942, 1035, 1114, 1410, 1459, 1619
MluNI	TGGCCA	3 cut(s)	42, 841, 1647
MlyI	GAGTC	3 cut(s)	163, 1094, 1190
MmeI	TCCRAC	2 cut(s)	69, 331
Mox20I	TGGCCA	3 cut(s)	42, 841, 1647
Mph1103I	ATGCAT	3 cut(s)	677, 787, 1864
MroXI	GAANNNNTTC	1 cut(s)	1585
MscI	TGGCCA	3 cut(s)	42, 841, 1647
MseI	TTAA	5 cut(s)	23, 222, 480, 1355, 1832
MslI	CAYNNNNRTG	2 cut(s)	670, 1254
Msp20I	TGGCCA	3 cut(s)	42, 841, 1647
MspA1I	CMGCKG	1 cut(s)	49
MspI	CCGG	4 cut(s)	18, 31, 235, 890
MspR9I	CCNGG	5 cut(s)	18, 236, 324, 1254, 1473
MunI	CAATTG	1 cut(s)	492
Mva1269I	GAATGC	2 cut(s)	645, 787
MvaI	CCWGG	3 cut(s)	324, 1254, 1473
MvnI	CGCG	1 cut(s)	1492
MwoI	GCNNNNNNNGC	6 cut(s)	193, 796, 1052, 1061, 1145, 1859
NciI	CCSGG	2 cut(s)	18, 236
NdeI	CATATG	2 cut(s)	764, 1918
NdeII	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
NheI	GCTAGC	1 cut(s)	1691
NlaIV	GGNNCC	6 cut(s)	14, 355, 900, 2050, 2154, 2170
NmuCI	GTSAC	1 cut(s)	1241
NsiI	ATGCAT	3 cut(s)	677, 787, 1864
NspI	RCATGY	1 cut(s)	365
OliI	CACNNNNGTG	1 cut(s)	1254
PaeR7I	CTCGAG	1 cut(s)	369
PctI	GAATGC	2 cut(s)	645, 787
PdmI	GAANNNNTTC	1 cut(s)	1585
PfeI	GAWTC	2 cut(s)	1783, 1967
PfoI	TCCNGGA	1 cut(s)	1471
PkrI	GCNGC	4 cut(s)	48, 1141, 1236, 1550
PleI	GAGTC	3 cut(s)	162, 1093, 1189
PpsI	GAGTC	3 cut(s)	162, 1093, 1189
PshBI	ATTAAT	1 cut(s)	1832
Psp124BI	GAGCTC	2 cut(s)	1959, 2249
Psp6I	CCWGG	3 cut(s)	322, 1252, 1471
PspFI	CCCAGC	2 cut(s)	1173, 1774
PspGI	CCWGG	3 cut(s)	322, 1252, 1471
PspN4I	GGNNCC	6 cut(s)	14, 355, 900, 2050, 2154, 2170
PspPI	GGNCC	5 cut(s)	83, 320, 353, 1303, 1913
PsrI	GAACNNNNNNTAC	2 cut(s)	959, 991
PstI	CTGCAG	1 cut(s)	1239
PstNI	CAGNNNCTG	1 cut(s)	446
PsuI	RGATCY	4 cut(s)	282, 898, 1422, 1452
RsaI	GTAC	1 cut(s)	994
RsaNI	GTAC	1 cut(s)	993
RseI	CAYNNNNRTG	2 cut(s)	670, 1254
Rsr2I	CGGWCCG	1 cut(s)	83
RsrII	CGGWCCG	1 cut(s)	83
SacI	GAGCTC	2 cut(s)	1959, 2249
SaqAI	TTAA	5 cut(s)	23, 222, 480, 1355, 1832
SatI	GCNGC	4 cut(s)	47, 1140, 1235, 1549
Sau3AI	GATC	8 cut(s)	102, 199, 282, 496, 898, 1422, 1452, 2125
Sau96I	GGNCC	5 cut(s)	83, 320, 353, 1303, 1913
ScaI	AGTACT	1 cut(s)	994
SchI	GAGTC	3 cut(s)	163, 1094, 1190
ScrFI	CCNGG	5 cut(s)	18, 236, 324, 1254, 1473
SduI	GDGCHC	5 cut(s)	17, 748, 1347, 1959, 2249
SfcI	CTRYAG	2 cut(s)	607, 1235
Sfr274I	CTCGAG	1 cut(s)	369
SinI	GGWCC	3 cut(s)	83, 353, 1913
SlaI	CTCGAG	1 cut(s)	369
SmiMI	CAYNNNNRTG	2 cut(s)	670, 1254
SmlI	CTYRAG	4 cut(s)	119, 369, 988, 2093
SmoI	CTYRAG	4 cut(s)	119, 369, 988, 2093
Sse9I	AATT	9 cut(s)	492, 624, 652, 942, 1035, 1114, 1410, 1459, 1619
SsiI	CCGC	3 cut(s)	49, 81, 658
SspMI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1692
SstI	GAGCTC	2 cut(s)	1959, 2249
StyD4I	CCNGG	5 cut(s)	16, 234, 322, 1252, 1471
StyI	CCWWGG	1 cut(s)	914
TaaI	ACNGT	4 cut(s)	589, 1585, 1841, 2012
TaiI	ACGT	2 cut(s)	293, 1528
TaqI	TCGA	4 cut(s)	198, 370, 1959, 2031
TasI	AATT	9 cut(s)	492, 624, 652, 942, 1035, 1114, 1410, 1459, 1619
TatI	WGTACW	1 cut(s)	992
TfiI	GAWTC	2 cut(s)	1783, 1967
Tru1I	TTAA	5 cut(s)	23, 222, 480, 1355, 1832
Tru9I	TTAA	5 cut(s)	23, 222, 480, 1355, 1832
TscAI	CASTG	5 cut(s)	753, 836, 1141, 1244, 2068
TseFI	GTSAC	1 cut(s)	1241
TseI	GCWGC	4 cut(s)	46, 1139, 1234, 1548
Tsp45I	GTSAC	1 cut(s)	1241
TspGWI	ACGGA	5 cut(s)	75, 443, 498, 1181, 1415
TspRI	CASTG	5 cut(s)	753, 836, 1141, 1244, 2068
VpaK11BI	GGWCC	3 cut(s)	83, 353, 1913
VspI	ATTAAT	1 cut(s)	1832
XapI	RAATTY	3 cut(s)	624, 652, 1035
XceI	RCATGY	1 cut(s)	365
XhoI	CTCGAG	1 cut(s)	369
XmiI	GTMKAC	2 cut(s)	1389, 1397
XmnI	GAANNNNTTC	1 cut(s)	1585
XspI	CTAG	6 cut(s)	308, 635, 800, 881, 972, 1692
ZrmI	AGTACT	1 cut(s)	994
Zsp2I	ATGCAT	3 cut(s)	677, 787, 1864

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.