FvH4_5g16830

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007038
Physical Location & Seq
Reverse (-)
9619278 .. 9623634
4357 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16830.t1

Sequence Viewer

Length: 1119 bp
ATGTCGGCGATCGGAAACGGATTGGGGAATTCGAATGATTCGGCGGCGGCGTTTGTGACGATGGCGAGCCAGCGGCTGCGGCTGAACCCTAACAAGGAGCACAAGCCGGAAAGCTACGACGACATGCAATTGGATTTCAGCCCGTCGATTTTCAGCTCGCTGGAGCGGTACTTGCCGCCGAACATGCTCGGAATGCCGAGACATGATAAGGTCAAGTTCATGAGGGAGATTCTCCTCAAGTACTTGCCCCATGGAGAACGCAACAGAGTTCAGAAGCACAGAGATTACAGGCAGAAGATTATATTGAATTACCAGCCTTTGCATAAAGAGTTATACCAGCTCGATCCCACCATCACCTTTGTCCCGACATTTCTAGAAGCAGTGAATGAGAATACTGAGGAAAGCTTCAGAAGGATATGTTCTGAACCCTCACCTGGAATATATACTTTTGAGATTTTTCAGCCACGTTTCTGTGAATTGTTATTAGCCGAGGTGGAGAATTTTGAAAAATGGGTTGGTGACTCCAAATATAGAATCATGCGACCCAATACAATGAATAAGTATGGTGCTGTTCTTGATGATTTTGGCCTTGAAACCATGCTGGACAAGCTTATGGAGGGTTTTGTTCGTCCTATAGCTAAAGAGCTGTTTCCTGAGGTTGGTGGAGCAACTTTGGATTCTCATCATGGGTTTGTGGTTGAATATGGTAACAATAGGGATCTTGACTTGGGTTTTCATGTTGATGACTCAGAAGTAACTTTGAATGTGTGCTTGGGAAAACAATTTACTGGAGGAGAGTTGTATTTCCGGGGAATACGTTGTGATAAACATGTAAATAGCGGAAGCCGTCCAGAGGAAGTCTTTGATTATTCTCATACCCCAGGATATGCTGTGCTTCATCGTGGTCGTCACAGGCATGGTGCTAAAGCCACGACATCAGGTCATCGTGTCAATTTACTTCTATGGTGCAGGAGTTCTGTTTTCAGAGAGATGAGGAAGTATCAGAAAGATTTCTCTACCTGGTGTGGGGAATGCTTGCATGAGAAGAAAGAGAGGCAACGAATGTCAATTACAGCTACCAAACAGGAGTTGTTGCTCAGGAAGGATGATGAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

373

Amino Acids

43.16

Weight (kDa)

6.95

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 100 - 222 4.4e-53 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 166
AciI CCGC 7 cut(s) 44, 47, 73, 79, 166, 176, 840
AclWI GGATC 2 cut(s) 338, 726
AcsI RAATTY 2 cut(s) 28, 499
AcuI CTGAAG 1 cut(s) 391
AfaI GTAC 2 cut(s) 170, 242
AfiI CCNNNNNNNGG 5 cut(s) 94, 434, 659, 853, 1026
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 5 cut(s) 307, 506, 593, 701, 763
AhdI GACNNNNNGTC 1 cut(s) 939
AjnI CCWGG 3 cut(s) 433, 880, 1019
AjuI GAANNNNNNNTTGG 4 cut(s) 498, 530, 755, 787
AluBI AGCT 8 cut(s) 114, 156, 340, 405, 610, 638, 646, 1076
AluI AGCT 8 cut(s) 114, 156, 340, 405, 610, 638, 646, 1076
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 2 cut(s) 338, 726
AlwNI CAGNNNCTG 1 cut(s) 76
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 1 cut(s) 76
ApoI RAATTY 2 cut(s) 28, 499
Asp700I GAANNNNTTC 1 cut(s) 1010
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 3 cut(s) 346, 423, 530
AsuII TTCGAA 1 cut(s) 32
AxyI CCTNAGG 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 63
BccI CCATC 2 cut(s) 55, 359
BceAI ACGGC 1 cut(s) 831
BciT130I CCWGG 3 cut(s) 435, 882, 1021
BcnI CCSGG 1 cut(s) 809
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 374
BfmI CTRYAG 1 cut(s) 633
BisI GCNGC 6 cut(s) 45, 48, 74, 77, 80, 176
BlsI GCNGC 6 cut(s) 46, 49, 75, 78, 81, 177
BmcAI AGTACT 1 cut(s) 242
Bme1390I CCNGG 4 cut(s) 435, 809, 882, 1021
BmeRI GACNNNNNGTC 1 cut(s) 939
BmrFI CCNGG 4 cut(s) 435, 809, 882, 1021
BpmI CTGGAG 2 cut(s) 182, 810
Bpu10I CCTNAGC 1 cut(s) 1097
Bpu14I TTCGAA 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 681
BsaJI CCNNGG 4 cut(s) 250, 489, 808, 880
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 434, 659, 853, 1026
Bse1I ACTGG 1 cut(s) 793
Bse21I CCTNAGG 1 cut(s) 654
Bse8I GATNNNNATC 1 cut(s) 681
BseBI CCWGG 3 cut(s) 435, 882, 1021
BseDI CCNNGG 4 cut(s) 250, 489, 808, 880
BseGI GGATG 1 cut(s) 1111
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 5 cut(s) 94, 434, 659, 853, 1026
BseMII CTCAG 4 cut(s) 387, 645, 762, 1111
BseNI ACTGG 1 cut(s) 793
BseRI GAGGAG 2 cut(s) 224, 807
BseXI GCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 988
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 588
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 2 cut(s) 107, 808
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 5 cut(s) 94, 434, 659, 853, 1026
BsmAI GTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 347
BsmI GAATGC 2 cut(s) 198, 1037
BsnI GGCC 1 cut(s) 588
Bsp119I TTCGAA 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 3 cut(s) 9, 343, 718
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 7 cut(s) 44, 47, 73, 79, 166, 176, 840
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 4 cut(s) 388, 646, 761, 1110
BspHI TCATGA 1 cut(s) 219
BspPI GGATC 2 cut(s) 338, 726
BspT104I TTCGAA 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 166
BsrI ACTGG 1 cut(s) 793
BssECI CCNNGG 4 cut(s) 250, 489, 808, 880
BssMI GATC 3 cut(s) 9, 343, 718
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 435, 882, 1021
BstBI TTCGAA 1 cut(s) 32
BstC8I GCNNGC 4 cut(s) 67, 71, 158, 1037
BstDEI CTNAG 4 cut(s) 396, 654, 748, 1097
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 1111
BstKTI GATC 3 cut(s) 12, 346, 721
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 3 cut(s) 9, 343, 718
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 5 cut(s) 79, 172, 184, 193, 607
BstNI CCWGG 3 cut(s) 435, 882, 1021
BstNSI RCATGY 3 cut(s) 127, 187, 833
BstSCI CCNGG 4 cut(s) 433, 807, 880, 1019
BstSFI CTRYAG 1 cut(s) 633
BstV1I GCAGC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
Bsu36I CCTNAGG 1 cut(s) 654
BsuRI GGCC 1 cut(s) 588
BtgI CCRYGG 1 cut(s) 250
BtsCI GGATG 1 cut(s) 1111
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 387
Cac8I GCNNGC 4 cut(s) 67, 71, 158, 1037
CaiI CAGNNNCTG 1 cut(s) 76
CciI TCATGA 1 cut(s) 219
CsiI ACCWGGT 1 cut(s) 1019
Csp6I GTAC 2 cut(s) 169, 241
CviQI GTAC 2 cut(s) 169, 241
DdeI CTNAG 4 cut(s) 396, 654, 748, 1097
DpnI GATC 3 cut(s) 11, 345, 720
DpnII GATC 3 cut(s) 9, 343, 718
DriI GACNNNNNGTC 1 cut(s) 939
Eam1105I GACNNNNNGTC 1 cut(s) 939
Eco130I CCWWGG 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 391
Eco81I CCTNAGG 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 3 cut(s) 433, 880, 1019
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaqI GGGAC 1 cut(s) 347
Fnu4HI GCNGC 6 cut(s) 45, 48, 74, 77, 80, 176
Fsp4HI GCNGC 6 cut(s) 45, 48, 74, 77, 80, 176
FspBI CTAG 1 cut(s) 374
GluI GCNGC 6 cut(s) 45, 48, 74, 77, 80, 176
GsuI CTGGAG 2 cut(s) 182, 810
HaeIII GGCC 1 cut(s) 588
HapII CCGG 2 cut(s) 107, 808
HindIII AAGCTT 2 cut(s) 403, 608
HinfI GANTC 7 cut(s) 38, 229, 521, 534, 677, 746, 1112
HpaII CCGG 2 cut(s) 107, 808
HphI GGTGA 3 cut(s) 346, 423, 530
Hpy188I TCNGA 8 cut(s) 14, 191, 273, 410, 424, 751, 986, 1005
Hpy188III TCNNGA 8 cut(s) 220, 364, 374, 575, 653, 722, 851, 1099
Hpy99I CGWCG 2 cut(s) 122, 148
HpyAV CCTTC 2 cut(s) 405, 1096
HpyCH4IV ACGT 2 cut(s) 466, 817
HpyCH4V TGCA 4 cut(s) 127, 322, 969, 1039
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 172, 184, 193, 607
HpyF3I CTNAG 4 cut(s) 396, 654, 748, 1097
HpySE526I ACGT 2 cut(s) 466, 817
Kzo9I GATC 3 cut(s) 9, 343, 718
LmnI GCTCC 3 cut(s) 97, 163, 665
Lsp1109I GCAGC 1 cut(s) 63
MabI ACCWGGT 1 cut(s) 1019
MaeI CTAG 1 cut(s) 374
MaeII ACGT 2 cut(s) 466, 817
MaeIII GTNAC 5 cut(s) 55, 518, 707, 754, 908
MalI GATC 3 cut(s) 11, 345, 720
MbiI CCGCTC 1 cut(s) 166
MboI GATC 3 cut(s) 9, 343, 718
MboII GAAGA 2 cut(s) 307, 1057
MfeI CAATTG 1 cut(s) 128
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 8 cut(s) 28, 128, 307, 476, 499, 782, 952, 1068
MlyI GAGTC 2 cut(s) 515, 740
MroXI GAANNNNTTC 1 cut(s) 1010
MslI CAYNNNNRTG 2 cut(s) 741, 915
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 2 cut(s) 107, 808
MspR9I CCNGG 4 cut(s) 435, 809, 882, 1021
MunI CAATTG 1 cut(s) 128
Mva1269I GAATGC 2 cut(s) 198, 1037
MvaI CCWGG 3 cut(s) 435, 882, 1021
MwoI GCNNNNNNNGC 5 cut(s) 79, 172, 184, 193, 607
NciI CCSGG 1 cut(s) 809
NcoI CCATGG 1 cut(s) 250
NdeII GATC 3 cut(s) 9, 343, 718
NmeAIII GCCGAG 2 cut(s) 222, 514
NmuCI GTSAC 3 cut(s) 55, 518, 908
NspI RCATGY 3 cut(s) 127, 187, 833
NspV TTCGAA 1 cut(s) 32
PagI TCATGA 1 cut(s) 219
PciI ACATGT 1 cut(s) 829
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PctI GAATGC 2 cut(s) 198, 1037
PdmI GAANNNNTTC 1 cut(s) 1010
PfeI GAWTC 4 cut(s) 38, 229, 534, 677
PkrI GCNGC 6 cut(s) 46, 49, 75, 78, 81, 177
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 2 cut(s) 515, 740
PpsI GAGTC 2 cut(s) 515, 740
PscI ACATGT 1 cut(s) 829
Psp6I CCWGG 3 cut(s) 433, 880, 1019
PspGI CCWGG 3 cut(s) 433, 880, 1019
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 718
PvuI CGATCG 1 cut(s) 12
RsaI GTAC 2 cut(s) 170, 242
RsaNI GTAC 2 cut(s) 169, 241
RseI CAYNNNNRTG 2 cut(s) 741, 915
SatI GCNGC 6 cut(s) 45, 48, 74, 77, 80, 176
Sau3AI GATC 3 cut(s) 9, 343, 718
ScaI AGTACT 1 cut(s) 242
SchI GAGTC 2 cut(s) 515, 740
ScrFI CCNGG 4 cut(s) 435, 809, 882, 1021
SduI GDGCHC 1 cut(s) 102
SexAI ACCWGGT 1 cut(s) 1019
SfcI CTRYAG 1 cut(s) 633
SfuI TTCGAA 1 cut(s) 32
SmiMI CAYNNNNRTG 2 cut(s) 741, 915
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 8 cut(s) 28, 128, 307, 476, 499, 782, 952, 1068
SsiI CCGC 7 cut(s) 44, 47, 73, 79, 166, 176, 840
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 4 cut(s) 433, 807, 880, 1019
StyI CCWWGG 1 cut(s) 250
TaiI ACGT 2 cut(s) 469, 820
TaqI TCGA 3 cut(s) 32, 146, 342
TasI AATT 8 cut(s) 28, 128, 307, 476, 499, 782, 952, 1068
TatI WGTACW 1 cut(s) 240
TauI GCSGC 5 cut(s) 47, 50, 76, 82, 178
TfiI GAWTC 4 cut(s) 38, 229, 534, 677
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 3 cut(s) 55, 518, 908
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 3 cut(s) 55, 518, 908
TspDTI ATGAA 4 cut(s) 208, 569, 725, 887
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 1 cut(s) 387
XapI RAATTY 2 cut(s) 28, 499
XbaI TCTAGA 1 cut(s) 373
XceI RCATGY 3 cut(s) 127, 187, 833
XmnI GAANNNNTTC 1 cut(s) 1010
XspI CTAG 1 cut(s) 374
ZrmI AGTACT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.