Rmu_sc0006939.1_g000004

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006939.1
OG0007038
Physical Location & Seq
Forward (+)
3218 .. 7912
4695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006939.1_g000004.1.cds

Sequence Viewer

Length: 1557 bp
atgtcggtcgacggccgggacggcccagctccggcggccggaaacggactggggaattcgaacaattcggcggcggcgttcgtgacgatgtcgagccagcggctgcggctgaaccctaacaaggagcacaagccggagagctacgacgacatgcaattggatttcagcccgtcgatcttcagctctctggaacggtacttgccgccgaacatgctcggaatgccgagatatgataaggtcaagttcatgagggagattctgctcaagtatttgcctcatggagagcgcaacagggttcagaagcatagggattacagacagaagatcatattgaattaccagcctttgcataaagagttatacctgctggatcctgccaccacctttgttcctgcatttctagaagcagttaatgagaatactgaggaaagcttcaaaaggatatgttctgaaccctcacctggaatatatacttttgagatttttcagccacggttctgtgaattgttattagccgaggtggagaattttgaaaaatgggtcggtgactccaaatatagaatcatgcgaccaaatacaatgaataggtatggtgcggttcttgatgattttggccttgaaaccatgcttgacaagcttttggagggttttgttcgtcctatagctaaagagctgtttcctgaggttggtggatcaactctggattctcatcatgggtttgtggttgaatatggtaacaatagggatctttacttgggttttcatgttgatgactcagaagtaactttgaatgtgtgcttggtacctgacctttgccccattctaaacttcagtacctcacgttcagataatatcaaaacgatacctcaggttctgaccccgaccgaagaatgtcccaacaatccactccctcctcctgggctttgctcccttggaggacccttaacggacgccgctcgccgaccaaacggcaccatcaaccgccgtctcatgagcttcctcgacttacgctcctccgccactccctcagccccctacaaaggcgtcaccacctccgacgtcaccgtcgaccaaacctgcaagctctggttccgcttcttcgtcccttctacgacatcgtcttcccccgcctctttacccgtcgtctttttcttccacggcagcggcttcagctttctcagccccgactcctacgcctacgacgccgtctgccgcaaattggcctgcgaaatctccgtcgtcgtagtctccgtcaactaccacctctccccagagcacggctacccctgccagtacgacgacggcctcgacaaattcctcgaccaaaaccccgactccctcctcgacctcgctgactgctcccgctgcttcctctttggctcttcaatctcacttccaagattctcactctccgattcgcaaccgtcgccggagccctccgccgccgccgattcccaaacccggcgccccgtcttcttcttcttcttcttcttcccgctcggcgtcgataattctctcggttgctgcctgtcttcgtcgtccgtggttagggcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

518

Amino Acids

57.93

Weight (kDa)

5.33

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1064
AatII GACGTC 1 cut(s) 1062
Acc36I ACCTGC 2 cut(s) 372, 1085
Acc65I GGTACC 1 cut(s) 802
AccB1I GGYRCC 3 cut(s) 802, 971, 1464
AccBSI CCGCTC 2 cut(s) 956, 1498
AccI GTMKAC 2 cut(s) 9, 1068
AclWI GGATC 4 cut(s) 365, 378, 700, 753
AcoI YGGCCR 2 cut(s) 13, 36
AcsI RAATTY 3 cut(s) 55, 526, 1313
AcuI CTGAAG 3 cut(s) 163, 814, 1153
AcyI GRCGYC 6 cut(s) 951, 1044, 1059, 1203, 1465, 1503
AfaI GTAC 4 cut(s) 197, 804, 835, 1295
AgsI TTSAA 7 cut(s) 334, 436, 533, 620, 728, 790, 1386
AjnI CCWGG 2 cut(s) 460, 916
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
Alw21I GWGCWC 2 cut(s) 129, 1278
Alw26I GTCTC 2 cut(s) 992, 1252
AlwI GGATC 4 cut(s) 365, 378, 700, 753
AlwNI CAGNNNCTG 2 cut(s) 103, 874
AoxI GGCC 6 cut(s) 13, 22, 36, 613, 1221, 1303
ApeKI GCWGC 4 cut(s) 103, 1161, 1365, 1524
ApoI RAATTY 3 cut(s) 55, 526, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 1221, 1253
Asp718I GGTACC 1 cut(s) 802
AspLEI GCGC 2 cut(s) 288, 1467
AspS9I GGNCC 2 cut(s) 23, 938
AsuC2I CCSGG 2 cut(s) 17, 1462
AsuHPI GGTGA 4 cut(s) 450, 557, 1039, 1054
AsuII TTCGAA 1 cut(s) 59
AvaII GGWCC 1 cut(s) 938
AxyI CCTNAGG 2 cut(s) 681, 867
BaeI ACNNNNGTAYC 2 cut(s) 817, 850
BamHI GGATCC 1 cut(s) 370
BanI GGYRCC 3 cut(s) 802, 971, 1464
BanII GRGCYC 1 cut(s) 1437
BbsI GAAGAC 3 cut(s) 1113, 1465, 1524
Bbv12I GWGCWC 2 cut(s) 129, 1278
BbvCI CCTCAGC 1 cut(s) 1027
BbvI GCAGC 4 cut(s) 90, 1173, 1352, 1511
BccI CCATC 1 cut(s) 983
BceAI ACGGC 8 cut(s) 28, 37, 969, 985, 1174, 1190, 1294, 1318
BciT130I CCWGG 2 cut(s) 462, 918
BcnI CCSGG 2 cut(s) 17, 1462
BcoDI GTCTC 2 cut(s) 992, 1252
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 1468
BfuAI ACCTGC 2 cut(s) 372, 1085
BglI GCCNNNNNGGC 1 cut(s) 21
Bme1390I CCNGG 4 cut(s) 17, 462, 918, 1462
Bme18I GGWCC 1 cut(s) 938
BmgT120I GGNCC 2 cut(s) 23, 938
BmiI GGNNCC 7 cut(s) 372, 804, 940, 973, 1091, 1434, 1466
BmrFI CCNGG 4 cut(s) 17, 462, 918, 1462
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 3 cut(s) 1113, 1465, 1524
Bpu10I CCTNAGC 1 cut(s) 1027
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 17, 1462
BsaBI GATNNNNATC 1 cut(s) 708
BsaHI GRCGYC 6 cut(s) 951, 1044, 1059, 1203, 1465, 1503
BsaJI CCNNGG 6 cut(s) 491, 516, 917, 931, 1156, 1542
BsaXI ACNNNNNCTCC 6 cut(s) 995, 1025, 1250, 1280, 1319, 1349
Bse1I ACTGG 2 cut(s) 54, 1291
Bse21I CCTNAGG 2 cut(s) 681, 867
Bse8I GATNNNNATC 1 cut(s) 708
BseBI CCWGG 2 cut(s) 462, 918
BseDI CCNNGG 6 cut(s) 491, 516, 917, 931, 1156, 1542
BseJI GATNNNNATC 1 cut(s) 708
BseMII CTCAG 6 cut(s) 414, 672, 789, 881, 1041, 1192
BseNI ACTGG 2 cut(s) 54, 1291
BseRI GAGGAG 3 cut(s) 903, 1003, 1331
BseX3I CGGCCG 2 cut(s) 13, 36
BseXI GCAGC 4 cut(s) 90, 1173, 1352, 1511
BseYI CCCAGC 1 cut(s) 25
Bsh1285I CGRYCG 4 cut(s) 9, 16, 39, 885
BshFI GGCC 6 cut(s) 15, 24, 38, 615, 1223, 1305
BshNI GGYRCC 3 cut(s) 802, 971, 1464
BsiEI CGRYCG 4 cut(s) 9, 16, 39, 885
BsiHKAI GWGCWC 2 cut(s) 129, 1278
BsiSI CCGG 6 cut(s) 16, 32, 39, 134, 1430, 1462
BslFI GGGAC 3 cut(s) 32, 879, 1088
BsmAI GTCTC 2 cut(s) 992, 1252
BsmBI CGTCTC 1 cut(s) 992
BsmFI GGGAC 3 cut(s) 32, 879, 1088
BsmI GAATGC 1 cut(s) 225
BsnI GGCC 6 cut(s) 15, 24, 38, 615, 1223, 1305
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 3 cut(s) 129, 1278, 1437
Bsp143I GATC 5 cut(s) 174, 324, 370, 692, 745
BspANI GGCC 6 cut(s) 15, 24, 38, 615, 1223, 1305
BspCNI CTCAG 6 cut(s) 415, 673, 788, 880, 1040, 1191
BspHI TCATGA 2 cut(s) 246, 990
BspLI GGNNCC 7 cut(s) 372, 804, 940, 973, 1091, 1434, 1466
BspMI ACCTGC 2 cut(s) 372, 1085
BspPI GGATC 4 cut(s) 365, 378, 700, 753
BspQI GCTCTTC 1 cut(s) 1387
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 3 cut(s) 802, 971, 1464
BsrBI CCGCTC 2 cut(s) 956, 1498
BsrI ACTGG 2 cut(s) 54, 1291
BssECI CCNNGG 6 cut(s) 491, 516, 917, 931, 1156, 1542
BssMI GATC 5 cut(s) 174, 324, 370, 692, 745
BssNI GRCGYC 6 cut(s) 951, 1044, 1059, 1203, 1465, 1503
BssT1I CCWWGG 1 cut(s) 931
Bst2UI CCWGG 2 cut(s) 462, 918
Bst4CI ACNGT 4 cut(s) 195, 495, 1066, 1425
Bst6I CTCTTC 1 cut(s) 1387
BstACI GRCGYC 6 cut(s) 951, 1044, 1059, 1203, 1465, 1503
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 98, 958, 1082, 1225
BstDEI CTNAG 7 cut(s) 423, 681, 775, 867, 1027, 1178, 1554
BstDSI CCRYGG 3 cut(s) 491, 1156, 1542
BstH2I RGCGCY 1 cut(s) 1468
BstHHI GCGC 2 cut(s) 288, 1467
BstKTI GATC 5 cut(s) 177, 327, 373, 695, 748
BstMAI GTCTC 2 cut(s) 992, 1252
BstMBI GATC 5 cut(s) 174, 324, 370, 692, 745
BstMCI CGRYCG 4 cut(s) 9, 16, 39, 885
BstNI CCWGG 2 cut(s) 462, 918
BstNSI RCATGY 2 cut(s) 154, 214
BstSCI CCNGG 4 cut(s) 15, 460, 916, 1460
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 4 cut(s) 90, 1173, 1352, 1511
BstV2I GAAGAC 3 cut(s) 1113, 1465, 1524
BstX2I RGATCY 2 cut(s) 370, 745
BstYI RGATCY 2 cut(s) 370, 745
BstZI CGGCCG 2 cut(s) 13, 36
Bsu36I CCTNAGG 2 cut(s) 681, 867
BsuRI GGCC 6 cut(s) 15, 24, 38, 615, 1223, 1305
BtgI CCRYGG 3 cut(s) 491, 1156, 1542
BveI ACCTGC 2 cut(s) 372, 1085
Cac8I GCNNGC 4 cut(s) 98, 958, 1082, 1225
CaiI CAGNNNCTG 2 cut(s) 103, 874
CciI TCATGA 2 cut(s) 246, 990
CfoI GCGC 2 cut(s) 288, 1467
Cfr13I GGNCC 2 cut(s) 23, 938
CseI GACGC 4 cut(s) 959, 1033, 1211, 1492
Csp6I GTAC 4 cut(s) 196, 803, 834, 1294
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 9 cut(s) 151, 211, 247, 278, 565, 625, 713, 764, 991
CviQI GTAC 4 cut(s) 196, 803, 834, 1294
DdeI CTNAG 7 cut(s) 423, 681, 775, 867, 1027, 1178, 1554
DinI GGCGCC 1 cut(s) 1466
DpnI GATC 5 cut(s) 176, 326, 372, 694, 747
DpnII GATC 5 cut(s) 174, 324, 370, 692, 745
DrdI GACNNNNNNGTC 1 cut(s) 1064
DseDI GACNNNNNNGTC 1 cut(s) 1064
EaeI YGGCCR 2 cut(s) 13, 36
EagI CGGCCG 2 cut(s) 13, 36
Eam1104I CTCTTC 1 cut(s) 1387
EarI CTCTTC 1 cut(s) 1387
EciI GGCGGA 2 cut(s) 1006, 1429
EclXI CGGCCG 2 cut(s) 13, 36
Eco130I CCWWGG 1 cut(s) 931
Eco24I GRGCYC 1 cut(s) 1437
Eco47I GGWCC 1 cut(s) 938
Eco52I CGGCCG 2 cut(s) 13, 36
Eco57I CTGAAG 3 cut(s) 163, 814, 1153
Eco81I CCTNAGG 2 cut(s) 681, 867
EcoO109I RGGNCCY 1 cut(s) 938
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 460, 916
EcoT14I CCWWGG 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 1437
EgeI GGCGCC 1 cut(s) 1466
EheI GGCGCC 1 cut(s) 1466
ErhI CCWWGG 1 cut(s) 931
Esp3I CGTCTC 1 cut(s) 992
FaeI CATG 9 cut(s) 154, 214, 250, 281, 568, 628, 716, 767, 994
FaqI GGGAC 3 cut(s) 32, 879, 1088
FatI CATG 9 cut(s) 150, 210, 246, 277, 564, 624, 712, 763, 990
FauI CCCGC 3 cut(s) 1135, 1370, 1503
FblI GTMKAC 2 cut(s) 9, 1068
FriOI GRGCYC 1 cut(s) 1437
FspBI CTAG 1 cut(s) 401
GlaI GCGC 2 cut(s) 287, 1466
GsaI CCCAGC 1 cut(s) 29
HaeII RGCGCY 1 cut(s) 1468
HaeIII GGCC 6 cut(s) 15, 24, 38, 615, 1223, 1305
HapII CCGG 6 cut(s) 16, 32, 39, 134, 1430, 1462
HgaI GACGC 4 cut(s) 959, 1033, 1211, 1492
HhaI GCGC 2 cut(s) 288, 1467
Hin1I GRCGYC 6 cut(s) 951, 1044, 1059, 1203, 1465, 1503
Hin1II CATG 9 cut(s) 154, 214, 250, 281, 568, 628, 716, 767, 994
Hin6I GCGC 2 cut(s) 286, 1465
HinP1I GCGC 2 cut(s) 286, 1465
HincII GTYRAC 3 cut(s) 10, 1069, 1255
HindII GTYRAC 3 cut(s) 10, 1069, 1255
HindIII AAGCTT 2 cut(s) 430, 635
HpaII CCGG 6 cut(s) 16, 32, 39, 134, 1430, 1462
HphI GGTGA 4 cut(s) 450, 557, 1039, 1054
Hpy166II GTNNAC 3 cut(s) 10, 1069, 1255
Hpy188I TCNGA 8 cut(s) 218, 300, 451, 778, 847, 876, 1057, 1414
Hpy188III TCNNGA 8 cut(s) 82, 188, 247, 401, 602, 680, 701, 991
Hpy8I GTNNAC 3 cut(s) 10, 1069, 1255
HpyAV CCTTC 1 cut(s) 1116
HpyCH4III ACNGT 4 cut(s) 195, 495, 1066, 1425
HpyCH4IV ACGT 2 cut(s) 841, 1059
HpyCH4V TGCA 4 cut(s) 154, 349, 395, 1080
HpyF3I CTNAG 7 cut(s) 423, 681, 775, 867, 1027, 1178, 1554
HpySE526I ACGT 2 cut(s) 841, 1059
Hsp92I GRCGYC 6 cut(s) 951, 1044, 1059, 1203, 1465, 1503
Hsp92II CATG 9 cut(s) 154, 214, 250, 281, 568, 628, 716, 767, 994
HspAI GCGC 2 cut(s) 286, 1465
KasI GGCGCC 1 cut(s) 1464
KpnI GGTACC 1 cut(s) 806
Kzo9I GATC 5 cut(s) 174, 324, 370, 692, 745
LguI GCTCTTC 1 cut(s) 1387
LmnI GCTCC 6 cut(s) 34, 124, 932, 1016, 1364, 1432
Lsp1109I GCAGC 4 cut(s) 90, 1173, 1352, 1511
MaeI CTAG 1 cut(s) 401
MaeII ACGT 2 cut(s) 841, 1059
MaeIII GTNAC 6 cut(s) 82, 545, 734, 781, 1045, 1060
MalI GATC 5 cut(s) 176, 326, 372, 694, 747
MbiI CCGCTC 2 cut(s) 956, 1498
MboI GATC 5 cut(s) 174, 324, 370, 692, 745
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 2 cut(s) 370, 745
MhlI GDGCHC 3 cut(s) 129, 1278, 1437
MluCI AATT 9 cut(s) 55, 64, 155, 334, 503, 526, 1217, 1313, 1510
Mly113I GGCGCC 1 cut(s) 1465
MlyI GAGTC 4 cut(s) 542, 767, 1181, 1328
MmeI TCCRAC 1 cut(s) 1080
MseI TTAA 2 cut(s) 411, 944
MslI CAYNNNNRTG 1 cut(s) 768
MspA1I CMGCKG 3 cut(s) 100, 1164, 1365
MspI CCGG 6 cut(s) 16, 32, 39, 134, 1430, 1462
MspR9I CCNGG 4 cut(s) 17, 462, 918, 1462
MunI CAATTG 1 cut(s) 155
Mva1269I GAATGC 1 cut(s) 225
MvaI CCWGG 2 cut(s) 462, 918
NarI GGCGCC 1 cut(s) 1465
NciI CCSGG 2 cut(s) 17, 1462
NdeII GATC 5 cut(s) 174, 324, 370, 692, 745
NlaIII CATG 9 cut(s) 154, 214, 250, 281, 568, 628, 716, 767, 994
NlaIV GGNNCC 7 cut(s) 372, 804, 940, 973, 1091, 1434, 1466
NmeAIII GCCGAG 3 cut(s) 249, 541, 1479
NmuCI GTSAC 4 cut(s) 82, 545, 1045, 1060
NspI RCATGY 2 cut(s) 154, 214
NspV TTCGAA 1 cut(s) 59
PagI TCATGA 2 cut(s) 246, 990
PciSI GCTCTTC 1 cut(s) 1387
PcsI WCGNNNNNNNCGW 4 cut(s) 74, 1065, 1248, 1305
PctI GAATGC 1 cut(s) 225
PfeI GAWTC 6 cut(s) 256, 561, 704, 1401, 1415, 1451
PflFI GACNNNGTC 3 cut(s) 88, 1117, 1205
PleI GAGTC 4 cut(s) 542, 767, 1181, 1328
PluTI GGCGCC 1 cut(s) 1468
PpsI GAGTC 4 cut(s) 542, 767, 1181, 1328
PpuMI RGGWCCY 1 cut(s) 938
Psp5II RGGWCCY 1 cut(s) 938
Psp6I CCWGG 2 cut(s) 460, 916
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 460, 916
PspN4I GGNNCC 7 cut(s) 372, 804, 940, 973, 1091, 1434, 1466
PspPI GGNCC 2 cut(s) 23, 938
PspPPI RGGWCCY 1 cut(s) 938
PsrI GAACNNNNNNTAC 2 cut(s) 826, 858
PstNI CAGNNNCTG 2 cut(s) 103, 874
PsuI RGATCY 2 cut(s) 370, 745
PsyI GACNNNGTC 3 cut(s) 88, 1117, 1205
RsaI GTAC 4 cut(s) 197, 804, 835, 1295
RsaNI GTAC 4 cut(s) 196, 803, 834, 1294
RseI CAYNNNNRTG 1 cut(s) 768
SalI GTCGAC 2 cut(s) 8, 1067
SapI GCTCTTC 1 cut(s) 1387
SaqAI TTAA 2 cut(s) 411, 944
Sau3AI GATC 5 cut(s) 174, 324, 370, 692, 745
Sau96I GGNCC 2 cut(s) 23, 938
SchI GAGTC 4 cut(s) 542, 767, 1181, 1328
ScrFI CCNGG 4 cut(s) 17, 462, 918, 1462
SduI GDGCHC 3 cut(s) 129, 1278, 1437
SfcI CTRYAG 1 cut(s) 660
SfiI GGCCNNNNNGGCC 1 cut(s) 21
SfoI GGCGCC 1 cut(s) 1466
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 1 cut(s) 938
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 9 cut(s) 55, 64, 155, 334, 503, 526, 1217, 1313, 1510
SspDI GGCGCC 1 cut(s) 1464
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 4 cut(s) 15, 460, 916, 1460
StyI CCWWGG 1 cut(s) 931
TaaI ACNGT 4 cut(s) 195, 495, 1066, 1425
TaiI ACGT 2 cut(s) 844, 1062
TaqII GACCGA 1 cut(s) 899
TasI AATT 9 cut(s) 55, 64, 155, 334, 503, 526, 1217, 1313, 1510
TfiI GAWTC 6 cut(s) 256, 561, 704, 1401, 1415, 1451
Tru1I TTAA 2 cut(s) 411, 944
Tru9I TTAA 2 cut(s) 411, 944
TseFI GTSAC 4 cut(s) 82, 545, 1045, 1060
TseI GCWGC 4 cut(s) 103, 1161, 1365, 1524
Tsp45I GTSAC 4 cut(s) 82, 545, 1045, 1060
TspDTI ATGAA 3 cut(s) 235, 596, 752
TspGWI ACGGA 5 cut(s) 60, 962, 1225, 1240, 1531
Tth111I GACNNNGTC 3 cut(s) 88, 1117, 1205
VpaK11BI GGWCC 1 cut(s) 938
XapI RAATTY 3 cut(s) 55, 526, 1313
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 2 cut(s) 154, 214
XmiI GTMKAC 2 cut(s) 9, 1068
XspI CTAG 1 cut(s) 401
ZraI GACGTC 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.