Prupe.5G226700_v2.0.a1

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0007038
Physical Location & Seq
Reverse (-)
17466203 .. 17470698
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G226700.6

Sequence Viewer

Length: 1164 bp
ATGTCGCTTGACGGCCGCCCCGGCGACCGCCTTGAAGGGCCCTTGCCGGTGACCGGGAACGGTGTCGTATTGGAGGACTCGAACAGGCCGGCGCTCGCACCCATGTCGAGCCAGAGGCTGAGATTGATCCCAAACAAGGATCACAATCCGGATAACTACGATGACATGGAATTGGATTTCAGTCCCTCGATTTTCACCTCGCTCGAGCGGTTCCTGCCTCCAAGCATGCTCGGGATGCCTCGACATGATAAGGTTAAGTTCATGACGGAGATTCTGCTCAAGTACTTGCCTCACGGAGAGCGTAACAGAGTTCAGAAACATAGAGACTACAGGCAGAAGATTATATCAAATTATCAGCCTTTACATAGAGAGTTATACAGTATGCACCCAGCAACCACCTTTGTGGCTCCATTTCTGAAAGCAGTTAGTGATAATACTGAGGAAAGCTTCAGAAGGATAATGTCTGAACCCTCGCCTGGGGTCTTTACATTTGAGATGTTTCAGCCACAGTTCTGTGAATTATTGTTGTCTGAGGTGGAGAATTTTGAAAAATGGGTTAATGAAGCCAAATTTAGAATCATGCGGCCAAATACAATGAATAAATATGGTGCTGTTCTTGATGATTTCGGCCTTGAAACCATGCTTGACAAGCTTATGGAGAATTTTATTCGTCCTATATCTAAAGTGTTCTTTGCTGAAGTTGGTGGATCAACTTTGGATTCTCACCATGGTTTTGTTGTTGAATATGGTGAAGATAGGGATGCTGACTTGGGTTTTCATGTGGACGACTCTGAAGTAACTTTGAATGTGTGCTTGGGTAAGCAATTTTCTGGAGGAGAATTGTTTTTTCGAGGCACGCGGTGTGATAAACATGTAAATAGTGGAAGCCATTCAGAGGAGATCTTTGATTATTCACATGTCCCAGGATGTGCTGTGCTTCATCGTGGCCGTCACCGGCATGGTGCTAGAGCAACGACATCAGGTCATCGCATCAATTTACTTCTATGGTGCAGAAGTTCTGTATTTAGAGAGATGAAGAAGTATCAGAAAGATTTCTCCAGCTGGTGTGGGGAATGCTTGCGTGAGAAGAAAGAGAGGCAATGCATATCAATCAGAGCTACCAAAAGGGAGTTGCTCAGGAGGGAAGGTGAATCCACGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

388

Amino Acids

44.62

Weight (kDa)

6.77

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 208
AccII CGCG 1 cut(s) 859
AccIII TCCGGA 1 cut(s) 148
AciI CCGC 5 cut(s) 16, 28, 208, 583, 859
AclWI GGATC 3 cut(s) 121, 147, 715
AcoI YGGCCR 3 cut(s) 13, 584, 946
AcsI RAATTY 3 cut(s) 541, 569, 661
AcuI CTGAAG 3 cut(s) 433, 717, 813
AdeI CACNNNGTG 1 cut(s) 861
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 5 cut(s) 53, 136, 476, 477, 895
AflIII ACRYGT 2 cut(s) 871, 916
AgsI TTSAA 5 cut(s) 35, 548, 635, 743, 805
AhdI GACNNNNNGTC 1 cut(s) 981
AjnI CCWGG 2 cut(s) 475, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 4 cut(s) 447, 652, 1062, 1118
AluI AGCT 4 cut(s) 447, 652, 1062, 1118
Alw26I GTCTC 1 cut(s) 318
AlwI GGATC 3 cut(s) 121, 147, 715
AlwNI CAGNNNCTG 1 cut(s) 118
Ama87I CYCGRG 2 cut(s) 203, 230
Aor13HI TCCGGA 1 cut(s) 148
AoxI GGCC 6 cut(s) 13, 38, 86, 584, 628, 946
ApaI GGGCCC 1 cut(s) 42
ApoI RAATTY 3 cut(s) 541, 569, 661
Asp700I GAANNNNTTC 2 cut(s) 889, 1052
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 2 cut(s) 38, 39
AsuC2I CCSGG 2 cut(s) 21, 55
AsuHPI GGTGA 6 cut(s) 61, 187, 716, 761, 944, 1160
AvaI CYCGRG 2 cut(s) 203, 230
BaeGI GKGCMC 1 cut(s) 42
BanII GRGCYC 1 cut(s) 42
BceAI ACGGC 2 cut(s) 28, 933
BcgI CGANNNNNNTGC 2 cut(s) 87, 121
BciT130I CCWGG 2 cut(s) 477, 924
BcnI CCSGG 2 cut(s) 21, 55
BcoDI GTCTC 1 cut(s) 318
BfaI CTAG 1 cut(s) 966
BfmI CTRYAG 1 cut(s) 328
BfoI RGCGCY 1 cut(s) 95
BglI GCCNNNNNGGC 1 cut(s) 21
BglII AGATCT 1 cut(s) 900
BisI GCNGC 2 cut(s) 16, 584
BlsI GCNGC 2 cut(s) 17, 585
BmcAI AGTACT 1 cut(s) 284
Bme1390I CCNGG 4 cut(s) 21, 55, 477, 924
BmeRI GACNNNNNGTC 1 cut(s) 981
BmeT110I CYCGRG 2 cut(s) 203, 230
BmgT120I GGNCC 2 cut(s) 38, 39
BmiI GGNNCC 3 cut(s) 40, 212, 408
BmrFI CCNGG 4 cut(s) 21, 55, 477, 924
BmsI GCATC 3 cut(s) 225, 751, 999
BpmI CTGGAG 2 cut(s) 852, 1042
Bpu10I CCTNAGC 1 cut(s) 1136
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 21, 55
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 5 cut(s) 19, 476, 727, 922, 1155
BsaWI WCCGGW 1 cut(s) 148
BsaXI ACNNNNNCTCC 2 cut(s) 1132, 1162
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 136, 476, 477, 895
Bse118I RCCGGY 3 cut(s) 46, 88, 954
Bse3DI GCAATG 1 cut(s) 1106
Bse8I GATNNNNATC 1 cut(s) 144
BseAI TCCGGA 1 cut(s) 148
BseBI CCWGG 2 cut(s) 477, 924
BseDI CCNNGG 5 cut(s) 19, 476, 727, 922, 1155
BseGI GGATG 3 cut(s) 240, 766, 932
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 5 cut(s) 53, 136, 476, 477, 895
BseMI GCAATG 1 cut(s) 1106
BseMII CTCAG 4 cut(s) 110, 429, 522, 1150
BseRI GAGGAG 2 cut(s) 849, 911
BseSI GKGCMC 1 cut(s) 42
BseX3I CGGCCG 1 cut(s) 13
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 1030
Bsh1236I CGCG 1 cut(s) 859
Bsh1285I CGRYCG 2 cut(s) 16, 28
BshFI GGCC 6 cut(s) 15, 40, 88, 586, 630, 948
BsiEI CGRYCG 2 cut(s) 16, 28
BsiHKCI CYCGRG 2 cut(s) 203, 230
BsiSI CCGG 6 cut(s) 21, 47, 54, 89, 149, 955
BslFI GGGAC 2 cut(s) 168, 905
BslI CCNNNNNNNGG 5 cut(s) 53, 136, 476, 477, 895
BsmAI GTCTC 1 cut(s) 318
BsmFI GGGAC 2 cut(s) 168, 905
BsmI GAATGC 1 cut(s) 1079
BsnI GGCC 6 cut(s) 15, 40, 88, 586, 630, 948
BsoBI CYCGRG 2 cut(s) 203, 230
Bsp120I GGGCCC 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 148
Bsp143I GATC 4 cut(s) 126, 139, 707, 900
Bsp19I CCATGG 1 cut(s) 727
BspACI CCGC 5 cut(s) 16, 28, 208, 583, 859
BspANI GGCC 6 cut(s) 15, 40, 88, 586, 630, 948
BspCNI CTCAG 4 cut(s) 111, 430, 523, 1149
BspEI TCCGGA 1 cut(s) 148
BspFNI CGCG 1 cut(s) 859
BspHI TCATGA 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 40, 212, 408
BspPI GGATC 3 cut(s) 121, 147, 715
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 1106
BsrFI RCCGGY 3 cut(s) 46, 88, 954
BssAI RCCGGY 3 cut(s) 46, 88, 954
BssECI CCNNGG 5 cut(s) 19, 476, 727, 922, 1155
BssMI GATC 4 cut(s) 126, 139, 707, 900
BssT1I CCWWGG 1 cut(s) 727
Bst2UI CCWGG 2 cut(s) 477, 924
Bst4CI ACNGT 3 cut(s) 62, 380, 510
BstC8I GCNNGC 5 cut(s) 90, 96, 227, 857, 1079
BstDEI CTNAG 4 cut(s) 119, 438, 531, 1136
BstDSI CCRYGG 2 cut(s) 727, 1155
BstEII GGTNACC 1 cut(s) 49
BstF5I GGATG 3 cut(s) 240, 766, 932
BstFNI CGCG 1 cut(s) 859
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 4 cut(s) 129, 142, 710, 903
BstMAI GTCTC 1 cut(s) 318
BstMBI GATC 4 cut(s) 126, 139, 707, 900
BstMCI CGRYCG 2 cut(s) 16, 28
BstMWI GCNNNNNNNGC 4 cut(s) 21, 214, 235, 649
BstNI CCWGG 2 cut(s) 477, 924
BstNSI RCATGY 3 cut(s) 229, 875, 920
BstPI GGTNACC 1 cut(s) 49
BstSCI CCNGG 4 cut(s) 19, 53, 475, 922
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 42
BstUI CGCG 1 cut(s) 859
BstX2I RGATCY 1 cut(s) 900
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 1 cut(s) 900
BstZI CGGCCG 1 cut(s) 13
BsuRI GGCC 6 cut(s) 15, 40, 88, 586, 630, 948
BtgI CCRYGG 2 cut(s) 727, 1155
BtgZI GCGATG 1 cut(s) 971
BtsCI GGATG 3 cut(s) 240, 766, 932
Cac8I GCNNGC 5 cut(s) 90, 96, 227, 857, 1079
CaiI CAGNNNCTG 1 cut(s) 118
CciI TCATGA 1 cut(s) 261
CfoI GCGC 1 cut(s) 94
Cfr10I RCCGGY 3 cut(s) 46, 88, 954
Cfr13I GGNCC 2 cut(s) 38, 39
Csp6I GTAC 1 cut(s) 283
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 4 cut(s) 119, 438, 531, 1136
DpnI GATC 4 cut(s) 128, 141, 709, 902
DpnII GATC 4 cut(s) 126, 139, 707, 900
DraIII CACNNNGTG 1 cut(s) 861
DriI GACNNNNNGTC 1 cut(s) 981
EaeI YGGCCR 3 cut(s) 13, 584, 946
EagI CGGCCG 1 cut(s) 13
Eam1105I GACNNNNNGTC 1 cut(s) 981
EclXI CGGCCG 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 727
Eco24I GRGCYC 1 cut(s) 42
Eco52I CGGCCG 1 cut(s) 13
Eco57I CTGAAG 3 cut(s) 433, 717, 813
Eco88I CYCGRG 2 cut(s) 203, 230
Eco91I GGTNACC 1 cut(s) 49
EcoO109I RGGNCCY 2 cut(s) 38, 39
EcoO65I GGTNACC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 475, 922
EcoT14I CCWWGG 1 cut(s) 727
EcoT22I ATGCAT 1 cut(s) 1106
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 727
FaqI GGGAC 2 cut(s) 168, 905
Fnu4HI GCNGC 2 cut(s) 16, 584
FokI GGATG 3 cut(s) 247, 773, 939
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 2 cut(s) 16, 584
FspBI CTAG 1 cut(s) 966
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 16, 584
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 2 cut(s) 852, 1042
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 6 cut(s) 15, 40, 88, 586, 630, 948
HapII CCGG 6 cut(s) 21, 47, 54, 89, 149, 955
HhaI GCGC 1 cut(s) 94
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HindIII AAGCTT 2 cut(s) 445, 650
HinfI GANTC 6 cut(s) 77, 271, 576, 719, 788, 1151
HpaII CCGG 6 cut(s) 21, 47, 54, 89, 149, 955
HphI GGTGA 6 cut(s) 61, 187, 716, 761, 944, 1160
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 9 cut(s) 315, 417, 452, 466, 532, 793, 895, 1047, 1115
Hpy188III TCNNGA 6 cut(s) 149, 232, 262, 617, 831, 1138
Hpy8I GTNNAC 1 cut(s) 784
HpyAV CCTTC 3 cut(s) 29, 447, 1139
HpyCH4III ACNGT 3 cut(s) 62, 380, 510
HpyCH4V TGCA 3 cut(s) 385, 1011, 1104
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 214, 235, 649
HpyF3I CTNAG 4 cut(s) 119, 438, 531, 1136
HspAI GCGC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 148
KroI GCCGGC 1 cut(s) 88
KroNI GCCGGC 1 cut(s) 90
Kzo9I GATC 4 cut(s) 126, 139, 707, 900
LmnI GCTCC 1 cut(s) 412
LweI GCATC 3 cut(s) 225, 751, 999
MaeI CTAG 1 cut(s) 966
MaeIII GTNAC 4 cut(s) 49, 302, 796, 950
MalI GATC 4 cut(s) 128, 141, 709, 902
MbiI CCGCTC 1 cut(s) 208
MboI GATC 4 cut(s) 126, 139, 707, 900
MboII GAAGA 4 cut(s) 349, 764, 1048, 1099
MflI RGATCY 1 cut(s) 900
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 9 cut(s) 170, 349, 518, 541, 569, 661, 824, 839, 994
MlyI GAGTC 2 cut(s) 71, 782
Mph1103I ATGCAT 1 cut(s) 1106
MroI TCCGGA 1 cut(s) 148
MroNI GCCGGC 1 cut(s) 88
MroXI GAANNNNTTC 2 cut(s) 889, 1052
MseI TTAA 2 cut(s) 255, 558
MslI CAYNNNNRTG 2 cut(s) 401, 957
MspA1I CMGCKG 1 cut(s) 1062
MspI CCGG 6 cut(s) 21, 47, 54, 89, 149, 955
MspR9I CCNGG 4 cut(s) 21, 55, 477, 924
Mva1269I GAATGC 1 cut(s) 1079
MvaI CCWGG 2 cut(s) 477, 924
MvnI CGCG 1 cut(s) 859
MwoI GCNNNNNNNGC 4 cut(s) 21, 214, 235, 649
NaeI GCCGGC 1 cut(s) 90
NciI CCSGG 2 cut(s) 21, 55
NcoI CCATGG 1 cut(s) 727
NdeII GATC 4 cut(s) 126, 139, 707, 900
NgoMIV GCCGGC 1 cut(s) 88
NlaIV GGNNCC 3 cut(s) 40, 212, 408
NmuCI GTSAC 2 cut(s) 49, 950
NsiI ATGCAT 1 cut(s) 1106
NspI RCATGY 3 cut(s) 229, 875, 920
OliI CACNNNNGTG 1 cut(s) 401
PaeI GCATGC 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 261
PciI ACATGT 2 cut(s) 871, 916
PctI GAATGC 1 cut(s) 1079
PdiI GCCGGC 1 cut(s) 90
PdmI GAANNNNTTC 2 cut(s) 889, 1052
PfeI GAWTC 4 cut(s) 271, 576, 719, 1151
PkrI GCNGC 2 cut(s) 17, 585
PleI GAGTC 2 cut(s) 71, 782
PpsI GAGTC 2 cut(s) 71, 782
PscI ACATGT 2 cut(s) 871, 916
Psp6I CCWGG 2 cut(s) 475, 922
PspEI GGTNACC 1 cut(s) 49
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 2 cut(s) 475, 922
PspN4I GGNNCC 3 cut(s) 40, 212, 408
PspOMI GGGCCC 1 cut(s) 38
PspPI GGNCC 2 cut(s) 38, 39
PspXI VCTCGAGB 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 900
PvuII CAGCTG 1 cut(s) 1062
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 2 cut(s) 401, 957
SaqAI TTAA 2 cut(s) 255, 558
SatI GCNGC 2 cut(s) 16, 584
Sau3AI GATC 4 cut(s) 126, 139, 707, 900
Sau96I GGNCC 2 cut(s) 38, 39
ScaI AGTACT 1 cut(s) 284
SchI GAGTC 2 cut(s) 71, 782
ScrFI CCNGG 4 cut(s) 21, 55, 477, 924
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 3 cut(s) 225, 751, 999
SfcI CTRYAG 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 2 cut(s) 401, 957
SmlI CTYRAG 2 cut(s) 203, 278
SmoI CTYRAG 2 cut(s) 203, 278
SphI GCATGC 1 cut(s) 229
Sse9I AATT 9 cut(s) 170, 349, 518, 541, 569, 661, 824, 839, 994
SsiI CCGC 5 cut(s) 16, 28, 208, 583, 859
SspMI CTAG 1 cut(s) 966
StyD4I CCNGG 4 cut(s) 19, 53, 475, 922
StyI CCWWGG 1 cut(s) 727
TaaI ACNGT 3 cut(s) 62, 380, 510
TaqI TCGA 6 cut(s) 80, 107, 188, 204, 241, 850
TasI AATT 9 cut(s) 170, 349, 518, 541, 569, 661, 824, 839, 994
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 18, 586
TfiI GAWTC 4 cut(s) 271, 576, 719, 1151
Tru1I TTAA 2 cut(s) 255, 558
Tru9I TTAA 2 cut(s) 255, 558
TseFI GTSAC 2 cut(s) 49, 950
Tsp45I GTSAC 2 cut(s) 49, 950
TspDTI ATGAA 6 cut(s) 250, 576, 611, 767, 929, 1049
TspGWI ACGGA 2 cut(s) 281, 309
XapI RAATTY 3 cut(s) 541, 569, 661
XceI RCATGY 3 cut(s) 229, 875, 920
XhoI CTCGAG 1 cut(s) 203
XmnI GAANNNNTTC 2 cut(s) 889, 1052
XspI CTAG 1 cut(s) 966
ZrmI AGTACT 1 cut(s) 284
Zsp2I ATGCAT 1 cut(s) 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.