Rw7G001800

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007038
Physical Location & Seq
Forward (+)
1459433 .. 1463640
4208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001800.1

Sequence Viewer

Length: 1152 bp
ATGTCGGTCGACGGCCGGGACGGCCCAGCTCCGGCGGCCGGAAACGGACTGGGGAATTCGAACAATTCGGCGGCGGCGTTCGTGACGATGTCGAGCCAGCGGCTGCGGCTGAACCCTAACAAGGAGCACAAGCCGGAGAGCTACGACGACATGCAATTGGATTTCAGCCCGTCGATCTTCAGCTCTCTGGAACGGTACTTGCCGCCGAACATGCTCGGAATGCCGAGATATGATAAGGTCAAGTTCATGAGGGAGATTCTGCTCAAGTATTTGCCTCATGGAGAGCGCAACAGGGTTCAGAAGCATAGGGATTACAGACAGAAGATCATATTGAATTACCAGCCTTTGCATAAAGAGTTATACCTGCTGGATCCTGCCACCACCTTTGTTCCTGCATTTCTAGAAGCAGTTAATGAGAATACTGAGGAAAGCTTCAAAAGGATATGTTCTGAACCCTCACCTGGAATATATACTTTTGAGATTTTTCAGCCACGGTTCTGTGAATTGTTATTAGCCGAGGTGGAGAATTTTGAAAAATGGGTCGGTGACTCCAAATATAGAATCATGCGACCAAATACAATGAATAGGTATGGTGCGGTTCTTGATGATTTTGGCCTTGAAACCATGCTTGACAAGCTTTTGGAGGGTTTTGTTCGCCCTATAGCTAAAGAGCTGTTTCCTGAGGTTGGTGGATCAACTCTGGATTCTCATCATGGGTTTGTGGTTGAATATGGTAACAATAGGGATCTTGACTTGGGTTTTCATGTTGATGACTCAGAAGTAACTTTGAATGTGTGCTTGGGTAAACAATTTTCTGGAGGAGAGTTGTATTTTCGGGGAATACGTTGTGATAAACATGTAAATAGCGGAAGCCGTCCAGAGGAAGTCTTTGATTATTCTCATACCCCAGGATATGCTGTGCTTCATCGTGGTCGTCACAGGCATGGTGCTAAAGCCACGACATCAGGTCATCGTGTCAATTTACTTCTATGGTGCAGGAGTTCTGTCTTCAGAGAGATGAGGAAGTATCAGAAAGATTTCTCTAACTGGTGTGGGGAATGCTTGCATGAGAAGAAAGAGAGGCAACGGATGTCAATTACAGCTACGAAACAGGAGTTGTTGCTCAGGAAGGATGATGAGTCAACCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

383

Amino Acids

44.1

Weight (kDa)

6.93

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 109 - 231 1.9e-51 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 372
AccI GTMKAC 1 cut(s) 9
AciI CCGC 9 cut(s) 35, 71, 74, 100, 106, 203, 596, 867, 1146
AclWI GGATC 4 cut(s) 365, 378, 700, 753
AcoI YGGCCR 2 cut(s) 13, 36
AcsI RAATTY 2 cut(s) 55, 526
AcuI CTGAAG 2 cut(s) 163, 994
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 6 cut(s) 31, 38, 121, 461, 686, 880
AflIII ACRYGT 1 cut(s) 856
AgsI TTSAA 6 cut(s) 334, 436, 533, 620, 728, 790
AhdI GACNNNNNGTC 1 cut(s) 966
AjnI CCWGG 2 cut(s) 460, 907
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AluBI AGCT 8 cut(s) 29, 141, 183, 432, 637, 665, 673, 1103
AluI AGCT 8 cut(s) 29, 141, 183, 432, 637, 665, 673, 1103
Alw21I GWGCWC 1 cut(s) 129
AlwI GGATC 4 cut(s) 365, 378, 700, 753
AlwNI CAGNNNCTG 1 cut(s) 103
AoxI GGCC 4 cut(s) 13, 22, 36, 613
ApeKI GCWGC 1 cut(s) 103
ApoI RAATTY 2 cut(s) 55, 526
Asp700I GAANNNNTTC 1 cut(s) 1037
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 450, 557
AsuII TTCGAA 1 cut(s) 59
AxyI CCTNAGG 1 cut(s) 681
BamHI GGATCC 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 1000
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 90
BceAI ACGGC 3 cut(s) 28, 37, 858
BciT130I CCWGG 2 cut(s) 462, 909
BcnI CCSGG 1 cut(s) 17
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 1 cut(s) 660
BfuAI ACCTGC 1 cut(s) 372
BglI GCCNNNNNGGC 1 cut(s) 21
BisI GCNGC 7 cut(s) 36, 72, 75, 101, 104, 107, 203
BlsI GCNGC 7 cut(s) 37, 73, 76, 102, 105, 108, 204
Bme1390I CCNGG 3 cut(s) 17, 462, 909
BmeRI GACNNNNNGTC 1 cut(s) 966
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 1 cut(s) 372
BmrFI CCNGG 3 cut(s) 17, 462, 909
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 1000
BpmI CTGGAG 1 cut(s) 837
Bpu10I CCTNAGC 1 cut(s) 1124
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 708
BsaJI CCNNGG 3 cut(s) 491, 516, 907
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 38, 121, 461, 686, 880
Bse1I ACTGG 2 cut(s) 54, 1052
Bse21I CCTNAGG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 708
BseBI CCWGG 2 cut(s) 462, 909
BseDI CCNNGG 3 cut(s) 491, 516, 907
BseGI GGATG 2 cut(s) 1095, 1138
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 6 cut(s) 31, 38, 121, 461, 686, 880
BseMII CTCAG 4 cut(s) 414, 672, 789, 1138
BseNI ACTGG 2 cut(s) 54, 1052
BseRI GAGGAG 1 cut(s) 834
BseX3I CGGCCG 2 cut(s) 13, 36
BseXI GCAGC 1 cut(s) 90
BseYI CCCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 1015
Bsh1285I CGRYCG 3 cut(s) 9, 16, 39
BshFI GGCC 4 cut(s) 15, 24, 38, 615
BsiEI CGRYCG 3 cut(s) 9, 16, 39
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 4 cut(s) 16, 32, 39, 134
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 6 cut(s) 31, 38, 121, 461, 686, 880
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 2 cut(s) 225, 1064
BsnI GGCC 4 cut(s) 15, 24, 38, 615
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 5 cut(s) 174, 324, 370, 692, 745
BspACI CCGC 9 cut(s) 35, 71, 74, 100, 106, 203, 596, 867, 1146
BspANI GGCC 4 cut(s) 15, 24, 38, 615
BspCNI CTCAG 4 cut(s) 415, 673, 788, 1137
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 372
BspMI ACCTGC 1 cut(s) 372
BspPI GGATC 4 cut(s) 365, 378, 700, 753
BspT104I TTCGAA 1 cut(s) 59
BsrI ACTGG 2 cut(s) 54, 1052
BssECI CCNNGG 3 cut(s) 491, 516, 907
BssMI GATC 5 cut(s) 174, 324, 370, 692, 745
Bst2UI CCWGG 2 cut(s) 462, 909
Bst4CI ACNGT 2 cut(s) 195, 495
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 98, 1064
BstDEI CTNAG 4 cut(s) 423, 681, 775, 1124
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 2 cut(s) 1095, 1138
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 5 cut(s) 177, 327, 373, 695, 748
BstMBI GATC 5 cut(s) 174, 324, 370, 692, 745
BstMCI CGRYCG 3 cut(s) 9, 16, 39
BstMWI GCNNNNNNNGC 6 cut(s) 21, 35, 106, 211, 220, 634
BstNI CCWGG 2 cut(s) 462, 909
BstNSI RCATGY 3 cut(s) 154, 214, 860
BstSCI CCNGG 3 cut(s) 15, 460, 907
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 1 cut(s) 90
BstV2I GAAGAC 1 cut(s) 1000
BstX2I RGATCY 2 cut(s) 370, 745
BstYI RGATCY 2 cut(s) 370, 745
BstZI CGGCCG 2 cut(s) 13, 36
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 4 cut(s) 15, 24, 38, 615
BtgI CCRYGG 1 cut(s) 491
BtsCI GGATG 2 cut(s) 1095, 1138
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 2 cut(s) 98, 1064
CaiI CAGNNNCTG 1 cut(s) 103
CciI TCATGA 1 cut(s) 246
CfoI GCGC 1 cut(s) 288
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 196
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 4 cut(s) 423, 681, 775, 1124
DpnI GATC 5 cut(s) 176, 326, 372, 694, 747
DpnII GATC 5 cut(s) 174, 324, 370, 692, 745
DriI GACNNNNNGTC 1 cut(s) 966
EaeI YGGCCR 2 cut(s) 13, 36
EagI CGGCCG 2 cut(s) 13, 36
Eam1105I GACNNNNNGTC 1 cut(s) 966
EclXI CGGCCG 2 cut(s) 13, 36
Eco52I CGGCCG 2 cut(s) 13, 36
Eco57I CTGAAG 2 cut(s) 163, 994
Eco81I CCTNAGG 1 cut(s) 681
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 460, 907
FaqI GGGAC 1 cut(s) 32
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 7 cut(s) 36, 72, 75, 101, 104, 107, 203
FokI GGATG 2 cut(s) 1102, 1145
Fsp4HI GCNGC 7 cut(s) 36, 72, 75, 101, 104, 107, 203
FspBI CTAG 1 cut(s) 401
GlaI GCGC 1 cut(s) 287
GluI GCNGC 7 cut(s) 36, 72, 75, 101, 104, 107, 203
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 837
HaeIII GGCC 4 cut(s) 15, 24, 38, 615
HapII CCGG 4 cut(s) 16, 32, 39, 134
HhaI GCGC 1 cut(s) 288
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 2 cut(s) 10, 1143
HindII GTYRAC 2 cut(s) 10, 1143
HindIII AAGCTT 2 cut(s) 430, 635
HinfI GANTC 6 cut(s) 256, 548, 561, 704, 773, 1139
HpaII CCGG 4 cut(s) 16, 32, 39, 134
HphI GGTGA 2 cut(s) 450, 557
Hpy166II GTNNAC 3 cut(s) 10, 806, 1143
Hpy188I TCNGA 6 cut(s) 218, 300, 451, 778, 1013, 1032
Hpy8I GTNNAC 3 cut(s) 10, 806, 1143
Hpy99I CGWCG 3 cut(s) 14, 149, 175
HpyAV CCTTC 1 cut(s) 1123
HpyCH4III ACNGT 2 cut(s) 195, 495
HpyCH4IV ACGT 1 cut(s) 844
HpyCH4V TGCA 5 cut(s) 154, 349, 395, 996, 1066
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 35, 106, 211, 220, 634
HpyF3I CTNAG 4 cut(s) 423, 681, 775, 1124
HpySE526I ACGT 1 cut(s) 844
HspAI GCGC 1 cut(s) 286
Kzo9I GATC 5 cut(s) 174, 324, 370, 692, 745
LmnI GCTCC 2 cut(s) 34, 124
Lsp1109I GCAGC 1 cut(s) 90
MaeI CTAG 1 cut(s) 401
MaeII ACGT 1 cut(s) 844
MaeIII GTNAC 5 cut(s) 82, 545, 734, 781, 935
MalI GATC 5 cut(s) 176, 326, 372, 694, 747
MboI GATC 5 cut(s) 174, 324, 370, 692, 745
MboII GAAGA 4 cut(s) 169, 334, 1000, 1084
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 2 cut(s) 370, 745
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 9 cut(s) 55, 64, 155, 334, 503, 526, 809, 979, 1095
MlyI GAGTC 3 cut(s) 542, 767, 1148
MroXI GAANNNNTTC 1 cut(s) 1037
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 2 cut(s) 768, 942
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 4 cut(s) 16, 32, 39, 134
MspR9I CCNGG 3 cut(s) 17, 462, 909
MunI CAATTG 1 cut(s) 155
Mva1269I GAATGC 2 cut(s) 225, 1064
MvaI CCWGG 2 cut(s) 462, 909
MwoI GCNNNNNNNGC 6 cut(s) 21, 35, 106, 211, 220, 634
NciI CCSGG 1 cut(s) 17
NdeII GATC 5 cut(s) 174, 324, 370, 692, 745
NlaIV GGNNCC 1 cut(s) 372
NmeAIII GCCGAG 2 cut(s) 249, 541
NmuCI GTSAC 3 cut(s) 82, 545, 935
NspI RCATGY 3 cut(s) 154, 214, 860
NspV TTCGAA 1 cut(s) 59
PagI TCATGA 1 cut(s) 246
PciI ACATGT 1 cut(s) 856
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 841
PctI GAATGC 2 cut(s) 225, 1064
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 3 cut(s) 256, 561, 704
PflFI GACNNNGTC 1 cut(s) 88
PkrI GCNGC 7 cut(s) 37, 73, 76, 102, 105, 108, 204
PleI GAGTC 3 cut(s) 542, 767, 1147
PpsI GAGTC 3 cut(s) 542, 767, 1147
PscI ACATGT 1 cut(s) 856
Psp6I CCWGG 2 cut(s) 460, 907
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 460, 907
PspN4I GGNNCC 1 cut(s) 372
PspPI GGNCC 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 103
PsuI RGATCY 2 cut(s) 370, 745
PsyI GACNNNGTC 1 cut(s) 88
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 2 cut(s) 768, 942
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 7 cut(s) 36, 72, 75, 101, 104, 107, 203
Sau3AI GATC 5 cut(s) 174, 324, 370, 692, 745
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 3 cut(s) 542, 767, 1148
ScrFI CCNGG 3 cut(s) 17, 462, 909
SduI GDGCHC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 660
SfiI GGCCNNNNNGGCC 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 768, 942
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 9 cut(s) 55, 64, 155, 334, 503, 526, 809, 979, 1095
SsiI CCGC 9 cut(s) 35, 71, 74, 100, 106, 203, 596, 867, 1146
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 3 cut(s) 15, 460, 907
TaaI ACNGT 2 cut(s) 195, 495
TaiI ACGT 1 cut(s) 847
TaqI TCGA 4 cut(s) 9, 59, 92, 173
TasI AATT 9 cut(s) 55, 64, 155, 334, 503, 526, 809, 979, 1095
TauI GCSGC 6 cut(s) 38, 74, 77, 103, 109, 205
TfiI GAWTC 3 cut(s) 256, 561, 704
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TseFI GTSAC 3 cut(s) 82, 545, 935
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 3 cut(s) 82, 545, 935
TspDTI ATGAA 4 cut(s) 235, 596, 752, 914
TspGWI ACGGA 2 cut(s) 60, 1102
Tth111I GACNNNGTC 1 cut(s) 88
XapI RAATTY 2 cut(s) 55, 526
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 3 cut(s) 154, 214, 860
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.