pycom14g19260

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007038
Physical Location & Seq
Reverse (-)
20658000 .. 20661187
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g19260.3

Sequence Viewer

Length: 1137 bp
ATGTCGCTCGAACCCCGCACGCCGGCGGCCGCGAACGGCGTCGTGTTGGAGGAACCGAAGCGTCCGGCGTTCGCGTCCCCGTCACACAGGCTGAGGCTGAACCCGAACAATGACCACAATCCGGATAACTACGACGACATGCAATTGGATTTCAGCCCCGAGATTTTCACCTCGCTCGAGCGGTACTTGCCGGCGAGCATGCTCGGGAAGCCCCGATACGATAAGTTCAAGTTCATGAGGGAGATTCTGCTCAAGTACTTTCCTCCCGGGGAGCGCAACAGAATTCAGAAACATAGAGAGTACAGGCAGAAGATTATATCAAATTATCAGCCTTTACATAGAGGGTTATACAGTATGCAACCTGCAGCCACCTTTGTTACGGCATTTCTGAAGGCAGTTAGTGAGAATACTCAGGAAAGCTTCAGAAGGATAATGTCTGAACCCTCACCAGGGATCTTTGCATTTGAGATGTTTCAGCCACGGTTCTGTGAACTTCTGTTGTCTGAGGTGGAGAATTTTGAAAATTGGGTCAATGAAGCCAAATTTAAAATCATGCGCCCAAATACAATGAACAAATATGGTGCCGTTCTTGATGATTTTGGCCTTGAAACCATGCTTGACAAGCTTATGGAGTCTTTTATTCGTCCTATATCTAAAGTGTTCTTTCCTGAAGTTGGTGGAGCAACTTTGGATTCTCATCATGGTTTTGTTGTTGAATATGGGGATAATAGGGATGTTGACTTGGGTTTTCATGTGGATGACTCTGAAGTGACCTTGAATGTGTGCTTGGGCAAGCAATTTACTGGAGGAGAATTGTATTTTCGGGGCACACGATGTGATAAACATGTAAATAGTGGAAGCCAGTCAGAGGAGATCTTTGATTATGCCCATGTTCCAGGATATGCGGTGCTTCATCGTGGCCGTCACCGGCACGGTGCTAGAGCTACAACATCTGGTCATCGCATCAATTTAATTCTATGGTGCAGAAGTTCCGTCTTCAGAGAGATGAAGAAATATCGGAAAGATTTCTCCAGCTGGTGTGGAGAATGCTTGCATGAGAAGAAAGAAAGGCAACGCAAGTCTATCGCAGCTACCAAACAGGAATTGCTCAGGAGGGACGGTGAATCTGCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

43.81

Weight (kDa)

8.6

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 581
AccBSI CCGCTC 1 cut(s) 181
AccII CGCG 2 cut(s) 32, 74
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 5 cut(s) 16, 26, 30, 181, 905
AclWI GGATC 1 cut(s) 461
AcoI YGGCCR 2 cut(s) 27, 919
AcsI RAATTY 3 cut(s) 282, 514, 542
AcuI CTGAAG 5 cut(s) 406, 410, 690, 786, 982
AcyI GRCGYC 1 cut(s) 39
AdeI CACNNNGTG 1 cut(s) 836
AfaI GTAC 3 cut(s) 185, 257, 302
AfiI CCNNNNNNNGG 6 cut(s) 22, 121, 449, 450, 674, 868
AflIII ACRYGT 1 cut(s) 844
AgsI TTSAA 5 cut(s) 229, 521, 608, 716, 778
AjnI CCWGG 2 cut(s) 448, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AluBI AGCT 5 cut(s) 420, 625, 944, 1035, 1091
AluI AGCT 5 cut(s) 420, 625, 944, 1035, 1091
AlwI GGATC 1 cut(s) 461
Ama87I CYCGRG 4 cut(s) 158, 176, 203, 266
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 3 cut(s) 27, 601, 919
ApeKI GCWGC 2 cut(s) 365, 1088
ApoI RAATTY 3 cut(s) 282, 514, 542
Asp700I GAANNNNTTC 1 cut(s) 1025
AspLEI GCGC 2 cut(s) 276, 558
AsuC2I CCSGG 2 cut(s) 267, 268
AsuHPI GGTGA 4 cut(s) 160, 438, 917, 1133
AvaI CYCGRG 4 cut(s) 158, 176, 203, 266
BaeGI GKGCMC 1 cut(s) 830
BanI GGYRCC 1 cut(s) 581
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
BbsI GAAGAC 1 cut(s) 988
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 377, 1100
BceAI ACGGC 4 cut(s) 52, 396, 569, 906
BcgI CGANNNNNNTGC 2 cut(s) 1066, 1100
BciT130I CCWGG 2 cut(s) 450, 897
BcnI CCSGG 2 cut(s) 267, 268
BfaI CTAG 1 cut(s) 939
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 370
BglII AGATCT 1 cut(s) 873
BisI GCNGC 4 cut(s) 27, 30, 366, 1089
BlsI GCNGC 4 cut(s) 28, 31, 367, 1090
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 4 cut(s) 267, 268, 450, 897
BmeT110I CYCGRG 4 cut(s) 158, 176, 203, 266
BmiI GGNNCC 2 cut(s) 54, 583
BmrFI CCNGG 4 cut(s) 267, 268, 450, 897
BmsI GCATC 1 cut(s) 972
BpiI GAAGAC 1 cut(s) 988
BpmI CTGGAG 2 cut(s) 825, 1015
Bpu10I CCTNAGC 2 cut(s) 92, 1109
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 2 cut(s) 267, 268
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 266, 267, 449, 479
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 1105, 1135
Bsc4I CCNNNNNNNGG 6 cut(s) 22, 121, 449, 450, 674, 868
Bse118I RCCGGY 3 cut(s) 22, 190, 927
Bse1I ACTGG 2 cut(s) 808, 862
Bse8I GATNNNNATC 1 cut(s) 696
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 2 cut(s) 450, 897
BseDI CCNNGG 4 cut(s) 266, 267, 449, 479
BseGI GGATG 2 cut(s) 739, 763
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 6 cut(s) 22, 121, 449, 450, 674, 868
BseMII CTCAG 4 cut(s) 83, 425, 495, 1123
BseNI ACTGG 2 cut(s) 808, 862
BseRI GAGGAG 2 cut(s) 822, 884
BseSI GKGCMC 1 cut(s) 830
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 377, 1100
BsgI GTGCAG 2 cut(s) 1003, 1113
Bsh1236I CGCG 2 cut(s) 32, 74
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 3 cut(s) 29, 603, 921
BshNI GGYRCC 1 cut(s) 581
BsiEI CGRYCG 1 cut(s) 30
BsiHKCI CYCGRG 4 cut(s) 158, 176, 203, 266
BsiSI CCGG 6 cut(s) 23, 65, 122, 191, 267, 928
BslFI GGGAC 2 cut(s) 61, 1130
BslI CCNNNNNNNGG 6 cut(s) 22, 121, 449, 450, 674, 868
BsmFI GGGAC 2 cut(s) 61, 1130
BsmI GAATGC 1 cut(s) 1052
BsnI GGCC 3 cut(s) 29, 603, 921
BsoBI CYCGRG 4 cut(s) 158, 176, 203, 266
Bsp1286I GDGCHC 1 cut(s) 830
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 2 cut(s) 453, 873
BspACI CCGC 5 cut(s) 16, 26, 30, 181, 905
BspANI GGCC 3 cut(s) 29, 603, 921
BspCNI CTCAG 4 cut(s) 84, 424, 496, 1122
BspEI TCCGGA 1 cut(s) 121
BspFNI CGCG 2 cut(s) 32, 74
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 54, 583
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 370
BspPI GGATC 1 cut(s) 461
BspT107I GGYRCC 1 cut(s) 581
BsrBI CCGCTC 1 cut(s) 181
BsrFI RCCGGY 3 cut(s) 22, 190, 927
BsrI ACTGG 2 cut(s) 808, 862
BssAI RCCGGY 3 cut(s) 22, 190, 927
BssECI CCNNGG 4 cut(s) 266, 267, 449, 479
BssMI GATC 2 cut(s) 453, 873
BssNI GRCGYC 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 450, 897
Bst4CI ACNGT 4 cut(s) 353, 483, 935, 1121
BstACI GRCGYC 1 cut(s) 39
BstC8I GCNNGC 7 cut(s) 20, 24, 192, 196, 200, 794, 1052
BstDEI CTNAG 4 cut(s) 92, 411, 504, 1109
BstDSI CCRYGG 1 cut(s) 479
BstF5I GGATG 2 cut(s) 739, 763
BstFNI CGCG 2 cut(s) 32, 74
BstHHI GCGC 2 cut(s) 276, 558
BstKTI GATC 2 cut(s) 456, 876
BstMBI GATC 2 cut(s) 453, 873
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 187, 208, 622
BstNI CCWGG 2 cut(s) 450, 897
BstNSI RCATGY 3 cut(s) 142, 202, 848
BstSCI CCNGG 4 cut(s) 265, 266, 448, 895
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 830
BstUI CGCG 2 cut(s) 32, 74
BstV1I GCAGC 2 cut(s) 377, 1100
BstV2I GAAGAC 1 cut(s) 988
BstX2I RGATCY 2 cut(s) 453, 873
BstYI RGATCY 2 cut(s) 453, 873
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 3 cut(s) 29, 603, 921
BtgI CCRYGG 1 cut(s) 479
BtgZI GCGATG 1 cut(s) 944
BtsCI GGATG 2 cut(s) 739, 763
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 7 cut(s) 20, 24, 192, 196, 200, 794, 1052
CciI TCATGA 1 cut(s) 234
CciNI GCGGCCGC 1 cut(s) 27
CfoI GCGC 2 cut(s) 276, 558
Cfr10I RCCGGY 3 cut(s) 22, 190, 927
Cfr9I CCCGGG 1 cut(s) 266
CseI GACGC 3 cut(s) 28, 50, 63
Csp6I GTAC 3 cut(s) 184, 256, 301
CviQI GTAC 3 cut(s) 184, 256, 301
DdeI CTNAG 4 cut(s) 92, 411, 504, 1109
DpnI GATC 2 cut(s) 455, 875
DpnII GATC 2 cut(s) 453, 873
DraI TTTAAA 1 cut(s) 547
DraIII CACNNNGTG 1 cut(s) 836
EaeI YGGCCR 2 cut(s) 27, 919
EagI CGGCCG 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 27
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 5 cut(s) 406, 410, 690, 786, 982
Eco88I CYCGRG 4 cut(s) 158, 176, 203, 266
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 2 cut(s) 448, 895
FaqI GGGAC 2 cut(s) 61, 1130
FauI CCCGC 1 cut(s) 23
Fnu4HI GCNGC 4 cut(s) 27, 30, 366, 1089
FokI GGATG 2 cut(s) 746, 770
Fsp4HI GCNGC 4 cut(s) 27, 30, 366, 1089
FspBI CTAG 1 cut(s) 939
GlaI GCGC 2 cut(s) 275, 557
GluI GCNGC 4 cut(s) 27, 30, 366, 1089
GsuI CTGGAG 2 cut(s) 825, 1015
HaeIII GGCC 3 cut(s) 29, 603, 921
HapII CCGG 6 cut(s) 23, 65, 122, 191, 267, 928
HgaI GACGC 3 cut(s) 28, 50, 63
HhaI GCGC 2 cut(s) 276, 558
Hin1I GRCGYC 1 cut(s) 39
Hin6I GCGC 2 cut(s) 274, 556
HinP1I GCGC 2 cut(s) 274, 556
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 418, 623
HinfI GANTC 5 cut(s) 244, 632, 692, 761, 1124
HpaII CCGG 6 cut(s) 23, 65, 122, 191, 267, 928
HphI GGTGA 4 cut(s) 160, 438, 917, 1133
Hpy166II GTNNAC 2 cut(s) 491, 739
Hpy188III TCNNGA 7 cut(s) 122, 205, 235, 413, 590, 668, 1111
Hpy8I GTNNAC 2 cut(s) 491, 739
Hpy99I CGWCG 2 cut(s) 44, 137
HpyAV CCTTC 2 cut(s) 385, 420
HpyCH4III ACNGT 4 cut(s) 353, 483, 935, 1121
HpyCH4V TGCA 7 cut(s) 142, 358, 365, 461, 984, 1054, 1130
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 208, 622
HpyF3I CTNAG 4 cut(s) 92, 411, 504, 1109
Hsp92I GRCGYC 1 cut(s) 39
HspAI GCGC 2 cut(s) 274, 556
Kpn2I TCCGGA 1 cut(s) 121
KroI GCCGGC 2 cut(s) 22, 190
KroNI GCCGGC 2 cut(s) 24, 192
Kzo9I GATC 2 cut(s) 453, 873
LmnI GCTCC 2 cut(s) 271, 680
Lsp1109I GCAGC 2 cut(s) 377, 1100
LweI GCATC 1 cut(s) 972
MaeI CTAG 1 cut(s) 939
MaeIII GTNAC 4 cut(s) 81, 376, 769, 923
MalI GATC 2 cut(s) 455, 875
MbiI CCGCTC 1 cut(s) 181
MboI GATC 2 cut(s) 453, 873
MboII GAAGA 4 cut(s) 322, 988, 1021, 1072
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 2 cut(s) 453, 873
MhlI GDGCHC 1 cut(s) 830
MlyI GAGTC 2 cut(s) 641, 755
MmeI TCCRAC 1 cut(s) 27
MreI CGCCGGCG 1 cut(s) 22
MroI TCCGGA 1 cut(s) 121
MroNI GCCGGC 2 cut(s) 22, 190
MroXI GAANNNNTTC 1 cut(s) 1025
MseI TTAA 2 cut(s) 546, 971
MslI CAYNNNNRTG 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 1035
MspI CCGG 6 cut(s) 23, 65, 122, 191, 267, 928
MspR9I CCNGG 4 cut(s) 267, 268, 450, 897
MunI CAATTG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 1052
MvaI CCWGG 2 cut(s) 450, 897
MvnI CGCG 2 cut(s) 32, 74
MwoI GCNNNNNNNGC 3 cut(s) 187, 208, 622
NaeI GCCGGC 2 cut(s) 24, 192
NciI CCSGG 2 cut(s) 267, 268
NdeII GATC 2 cut(s) 453, 873
NgoMIV GCCGGC 2 cut(s) 22, 190
NlaIV GGNNCC 2 cut(s) 54, 583
NmuCI GTSAC 3 cut(s) 81, 769, 923
NotI GCGGCCGC 1 cut(s) 27
NspI RCATGY 3 cut(s) 142, 202, 848
PaeI GCATGC 1 cut(s) 202
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 234
PciI ACATGT 1 cut(s) 844
PcsI WCGNNNNNNNCGW 1 cut(s) 829
PctI GAATGC 1 cut(s) 1052
PdiI GCCGGC 2 cut(s) 24, 192
PdmI GAANNNNTTC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 244, 692, 1124
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 4 cut(s) 28, 31, 367, 1090
PleI GAGTC 2 cut(s) 640, 755
PpsI GAGTC 2 cut(s) 640, 755
PscI ACATGT 1 cut(s) 844
Psp6I CCWGG 2 cut(s) 448, 895
PspGI CCWGG 2 cut(s) 448, 895
PspN4I GGNNCC 2 cut(s) 54, 583
PspXI VCTCGAGB 1 cut(s) 176
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 453, 873
PvuII CAGCTG 1 cut(s) 1035
RsaI GTAC 3 cut(s) 185, 257, 302
RsaNI GTAC 3 cut(s) 184, 256, 301
RseI CAYNNNNRTG 1 cut(s) 756
SaqAI TTAA 2 cut(s) 546, 971
SatI GCNGC 4 cut(s) 27, 30, 366, 1089
Sau3AI GATC 2 cut(s) 453, 873
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 2 cut(s) 641, 755
ScrFI CCNGG 4 cut(s) 267, 268, 450, 897
SduI GDGCHC 1 cut(s) 830
SfaNI GCATC 1 cut(s) 972
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 176
SgrAI CRCCGGYG 1 cut(s) 22
SlaI CTCGAG 1 cut(s) 176
SmaI CCCGGG 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 756
SmlI CTYRAG 2 cut(s) 176, 251
SmoI CTYRAG 2 cut(s) 176, 251
SphI GCATGC 1 cut(s) 202
SsiI CCGC 5 cut(s) 16, 26, 30, 181, 905
SspMI CTAG 1 cut(s) 939
StyD4I CCNGG 4 cut(s) 265, 266, 448, 895
TaaI ACNGT 4 cut(s) 353, 483, 935, 1121
TaqI TCGA 2 cut(s) 9, 177
TatI WGTACW 2 cut(s) 255, 300
TauI GCSGC 2 cut(s) 29, 32
TfiI GAWTC 3 cut(s) 244, 692, 1124
Tru1I TTAA 2 cut(s) 546, 971
Tru9I TTAA 2 cut(s) 546, 971
TseFI GTSAC 3 cut(s) 81, 769, 923
TseI GCWGC 2 cut(s) 365, 1088
Tsp45I GTSAC 3 cut(s) 81, 769, 923
TspDTI ATGAA 6 cut(s) 223, 549, 584, 740, 902, 1022
TspGWI ACGGA 1 cut(s) 982
TspMI CCCGGG 1 cut(s) 266
XapI RAATTY 3 cut(s) 282, 514, 542
XceI RCATGY 3 cut(s) 142, 202, 848
XhoI CTCGAG 1 cut(s) 176
XmaI CCCGGG 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 1025
XspI CTAG 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.