MD14G1231400.v1.1

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007038
Physical Location & Seq
Reverse (-)
31196087 .. 31199617
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1231400.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGTCGCTTGAACCCCGCACGCCGGCGGCCGCGAACGGCGTCGTGTCGGAGGAACCGAAGCGTCCGGCGTTCGCGTCCCCGTCACCCAGGCTGAGGCTGAACCCGAACAATGACCACAATCCGGATAACTACGACGACATGCAATTGGATTTCAGCCCCGAGATTTTCACCTCGCTCGAGCGGTACTTGCCGGCGAGCATGCTCGGGAAGCCCCGGTACGATAAGTTCAAGTTCATGAGGGAGATTCTGCTCAAGTACTTTCCTCCCGGAGAGCGCAACAGAGTTCAGAAACATAGAGAGTACAGGCAGAAGATTATATCAAATTATCAGCCTTTACATAGAGGGTTATACAGTATGCAACCTGCAGCCACCTTCGTTACGGCATTTCTGAAGGCAGTTAGTGAGAATACTGAGGAAAGCTTCAGAAGGATAATGTCTGAACCCTCACCAGGGATCTTTACATTTGAGATGTTTCAGCCGCGGTTCTGTGAACTTCTGTTGTCTGAGGTGGAGCATTTTGAAAATTGGGTCAATGAAGCCAAATTTAAAATCATGCGCCCAAATACAATGAACAAATATGGCGCCGTTCTTGATGATTTTGGCCTTGAAACCATGCTTGACAAGCTTGTGGAGTCTTTTATTCGTCCTATATCTAAAGTGTTCTATCCTGAAGTTGGTGGAGCAACTTTGGATTCTCATCATGGTTTTGTTGTTGAATATGGGGATAATAGGGATGTTGACTTGGGTTTTCATGTGGATGACTCTGAAGTGACCTTGAATGTGTGCTTGGGCAAGCAATTTTCTGGAGGAGAATTGTATTTTCGGGGCACACGATGTGATAAACATGTAAATAGTGGAAGCCAGTCAGAGGAGATCTTTGATTATGCCCATGTTCCAGGATATGCTGTGCTTCATCGTGGCCGTCACCGGCACGGTGCTAGAGCTACAACATCTGGTCATCGCATCAATTTAATTCTATGGTGCAGAAGTTCCGTCTTCAGAGAGATGAAGAAATATCAGAAAGATTTCTCCAGCTGGTGTGGAGAATGCTTGCTTGAGAAGAAAGAAAGGCAACGCAAGTCAATCGCAGCTACCAAACAGGAATTGCTCAGGAGGGACGGTGAATCCGCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

43.7

Weight (kDa)

8.12

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 105 - 227 2.8e-55 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 370
AccB1I GGYRCC 1 cut(s) 581
AccBSI CCGCTC 1 cut(s) 181
AccII CGCG 3 cut(s) 32, 74, 481
AccIII TCCGGA 1 cut(s) 121
AciI CCGC 7 cut(s) 16, 26, 30, 181, 479, 481, 1128
AclWI GGATC 1 cut(s) 461
AcoI YGGCCR 2 cut(s) 27, 919
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 5 cut(s) 406, 410, 690, 786, 982
AcyI GRCGYC 2 cut(s) 39, 582
AdeI CACNNNGTG 1 cut(s) 836
AfaI GTAC 4 cut(s) 185, 218, 257, 302
AfiI CCNNNNNNNGG 7 cut(s) 22, 93, 121, 449, 450, 674, 868
AflIII ACRYGT 1 cut(s) 844
AgsI TTSAA 6 cut(s) 11, 229, 521, 608, 716, 778
AjnI CCWGG 3 cut(s) 86, 448, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 770, 802
AluBI AGCT 5 cut(s) 420, 625, 944, 1035, 1091
AluI AGCT 5 cut(s) 420, 625, 944, 1035, 1091
AlwI GGATC 1 cut(s) 461
Ama87I CYCGRG 3 cut(s) 158, 176, 203
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 3 cut(s) 27, 601, 919
ApeKI GCWGC 2 cut(s) 365, 1088
ApoI RAATTY 1 cut(s) 542
Asp700I GAANNNNTTC 1 cut(s) 1025
AspLEI GCGC 3 cut(s) 276, 558, 584
AsuC2I CCSGG 2 cut(s) 214, 267
AsuHPI GGTGA 5 cut(s) 75, 160, 438, 917, 1133
AvaI CYCGRG 3 cut(s) 158, 176, 203
BaeGI GKGCMC 1 cut(s) 830
BanI GGYRCC 1 cut(s) 581
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
BbsI GAAGAC 1 cut(s) 988
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 377, 1100
BceAI ACGGC 4 cut(s) 52, 396, 569, 906
BcgI CGANNNNNNTGC 2 cut(s) 1066, 1100
BciT130I CCWGG 3 cut(s) 88, 450, 897
BcnI CCSGG 2 cut(s) 214, 267
BfaI CTAG 1 cut(s) 939
BfmI CTRYAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 370
BglII AGATCT 1 cut(s) 873
BisI GCNGC 5 cut(s) 27, 30, 366, 479, 1089
BlsI GCNGC 5 cut(s) 28, 31, 367, 480, 1090
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 5 cut(s) 88, 214, 267, 450, 897
BmeT110I CYCGRG 3 cut(s) 158, 176, 203
BmiI GGNNCC 2 cut(s) 54, 583
BmrFI CCNGG 5 cut(s) 88, 214, 267, 450, 897
BmsI GCATC 1 cut(s) 972
BpiI GAAGAC 1 cut(s) 988
BpmI CTGGAG 2 cut(s) 825, 1015
Bpu10I CCTNAGC 2 cut(s) 92, 1109
BpuEI CTTGAG 2 cut(s) 236, 1076
BpuMI CCSGG 2 cut(s) 214, 267
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 2 cut(s) 39, 582
BsaJI CCNNGG 4 cut(s) 86, 212, 449, 479
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 1105, 1135
Bsc4I CCNNNNNNNGG 7 cut(s) 22, 93, 121, 449, 450, 674, 868
Bse118I RCCGGY 3 cut(s) 22, 190, 927
Bse1I ACTGG 1 cut(s) 862
Bse8I GATNNNNATC 1 cut(s) 696
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 3 cut(s) 88, 450, 897
BseDI CCNNGG 4 cut(s) 86, 212, 449, 479
BseGI GGATG 2 cut(s) 739, 763
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 7 cut(s) 22, 93, 121, 449, 450, 674, 868
BseMII CTCAG 4 cut(s) 83, 402, 495, 1123
BseNI ACTGG 1 cut(s) 862
BseRI GAGGAG 2 cut(s) 822, 884
BseSI GKGCMC 1 cut(s) 830
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 377, 1100
BsgI GTGCAG 1 cut(s) 1003
Bsh1236I CGCG 3 cut(s) 32, 74, 481
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 3 cut(s) 29, 603, 921
BshNI GGYRCC 1 cut(s) 581
BsiEI CGRYCG 1 cut(s) 30
BsiHKCI CYCGRG 3 cut(s) 158, 176, 203
BsiSI CCGG 7 cut(s) 23, 65, 122, 191, 214, 267, 928
BslFI GGGAC 2 cut(s) 61, 1130
BslI CCNNNNNNNGG 7 cut(s) 22, 93, 121, 449, 450, 674, 868
BsmFI GGGAC 2 cut(s) 61, 1130
BsmI GAATGC 1 cut(s) 1052
BsnI GGCC 3 cut(s) 29, 603, 921
BsoBI CYCGRG 3 cut(s) 158, 176, 203
Bsp1286I GDGCHC 1 cut(s) 830
Bsp13I TCCGGA 1 cut(s) 121
Bsp143I GATC 2 cut(s) 453, 873
BspACI CCGC 7 cut(s) 16, 26, 30, 181, 479, 481, 1128
BspANI GGCC 3 cut(s) 29, 603, 921
BspCNI CTCAG 4 cut(s) 84, 403, 496, 1122
BspEI TCCGGA 1 cut(s) 121
BspFNI CGCG 3 cut(s) 32, 74, 481
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 54, 583
BspMAI CTGCAG 1 cut(s) 367
BspMI ACCTGC 1 cut(s) 370
BspPI GGATC 1 cut(s) 461
BspT107I GGYRCC 1 cut(s) 581
BsrBI CCGCTC 1 cut(s) 181
BsrFI RCCGGY 3 cut(s) 22, 190, 927
BsrI ACTGG 1 cut(s) 862
BssAI RCCGGY 3 cut(s) 22, 190, 927
BssECI CCNNGG 4 cut(s) 86, 212, 449, 479
BssMI GATC 2 cut(s) 453, 873
BssNI GRCGYC 2 cut(s) 39, 582
Bst2UI CCWGG 3 cut(s) 88, 450, 897
Bst4CI ACNGT 3 cut(s) 353, 935, 1121
BstACI GRCGYC 2 cut(s) 39, 582
BstC8I GCNNGC 7 cut(s) 20, 24, 192, 196, 200, 794, 1052
BstDEI CTNAG 4 cut(s) 92, 411, 504, 1109
BstDSI CCRYGG 1 cut(s) 479
BstF5I GGATG 2 cut(s) 739, 763
BstFNI CGCG 3 cut(s) 32, 74, 481
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 3 cut(s) 276, 558, 584
BstKTI GATC 2 cut(s) 456, 876
BstMBI GATC 2 cut(s) 453, 873
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 187, 208, 622
BstNI CCWGG 3 cut(s) 88, 450, 897
BstNSI RCATGY 3 cut(s) 142, 202, 848
BstSCI CCNGG 5 cut(s) 86, 212, 265, 448, 895
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 830
BstUI CGCG 3 cut(s) 32, 74, 481
BstV1I GCAGC 2 cut(s) 377, 1100
BstV2I GAAGAC 1 cut(s) 988
BstX2I RGATCY 2 cut(s) 453, 873
BstYI RGATCY 2 cut(s) 453, 873
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 3 cut(s) 29, 603, 921
BtgI CCRYGG 1 cut(s) 479
BtgZI GCGATG 1 cut(s) 944
BtsCI GGATG 2 cut(s) 739, 763
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 7 cut(s) 20, 24, 192, 196, 200, 794, 1052
CciI TCATGA 1 cut(s) 234
CciNI GCGGCCGC 1 cut(s) 27
CfoI GCGC 3 cut(s) 276, 558, 584
Cfr10I RCCGGY 3 cut(s) 22, 190, 927
Cfr42I CCGCGG 1 cut(s) 482
CseI GACGC 3 cut(s) 28, 50, 63
Csp6I GTAC 4 cut(s) 184, 217, 256, 301
CviAII CATG 9 cut(s) 139, 199, 235, 553, 613, 701, 752, 845, 890
CviQI GTAC 4 cut(s) 184, 217, 256, 301
DdeI CTNAG 4 cut(s) 92, 411, 504, 1109
DinI GGCGCC 1 cut(s) 583
DpnI GATC 2 cut(s) 455, 875
DpnII GATC 2 cut(s) 453, 873
DraI TTTAAA 1 cut(s) 547
DraIII CACNNNGTG 1 cut(s) 836
EaeI YGGCCR 2 cut(s) 27, 919
EagI CGGCCG 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 27
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 5 cut(s) 406, 410, 690, 786, 982
Eco88I CYCGRG 3 cut(s) 158, 176, 203
EcoRII CCWGG 3 cut(s) 86, 448, 895
EgeI GGCGCC 1 cut(s) 583
EheI GGCGCC 1 cut(s) 583
FaeI CATG 9 cut(s) 142, 202, 238, 556, 616, 704, 755, 848, 893
FaqI GGGAC 2 cut(s) 61, 1130
FatI CATG 9 cut(s) 138, 198, 234, 552, 612, 700, 751, 844, 889
FauI CCCGC 1 cut(s) 23
Fnu4HI GCNGC 5 cut(s) 27, 30, 366, 479, 1089
FokI GGATG 2 cut(s) 746, 770
Fsp4HI GCNGC 5 cut(s) 27, 30, 366, 479, 1089
FspBI CTAG 1 cut(s) 939
GlaI GCGC 3 cut(s) 275, 557, 583
GluI GCNGC 5 cut(s) 27, 30, 366, 479, 1089
GsuI CTGGAG 2 cut(s) 825, 1015
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 3 cut(s) 29, 603, 921
HapII CCGG 7 cut(s) 23, 65, 122, 191, 214, 267, 928
HgaI GACGC 3 cut(s) 28, 50, 63
HhaI GCGC 3 cut(s) 276, 558, 584
Hin1I GRCGYC 2 cut(s) 39, 582
Hin1II CATG 9 cut(s) 142, 202, 238, 556, 616, 704, 755, 848, 893
Hin6I GCGC 3 cut(s) 274, 556, 582
HinP1I GCGC 3 cut(s) 274, 556, 582
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 2 cut(s) 418, 623
HinfI GANTC 5 cut(s) 244, 632, 692, 761, 1124
HpaII CCGG 7 cut(s) 23, 65, 122, 191, 214, 267, 928
HphI GGTGA 5 cut(s) 75, 160, 438, 917, 1133
Hpy166II GTNNAC 2 cut(s) 491, 739
Hpy188III TCNNGA 7 cut(s) 122, 205, 235, 590, 668, 804, 1111
Hpy8I GTNNAC 2 cut(s) 491, 739
Hpy99I CGWCG 2 cut(s) 44, 137
HpyAV CCTTC 3 cut(s) 382, 385, 420
HpyCH4III ACNGT 3 cut(s) 353, 935, 1121
HpyCH4V TGCA 4 cut(s) 142, 358, 365, 984
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 208, 622
HpyF3I CTNAG 4 cut(s) 92, 411, 504, 1109
Hsp92I GRCGYC 2 cut(s) 39, 582
Hsp92II CATG 9 cut(s) 142, 202, 238, 556, 616, 704, 755, 848, 893
HspAI GCGC 3 cut(s) 274, 556, 582
KasI GGCGCC 1 cut(s) 581
Kpn2I TCCGGA 1 cut(s) 121
KroI GCCGGC 2 cut(s) 22, 190
KroNI GCCGGC 2 cut(s) 24, 192
KspI CCGCGG 1 cut(s) 482
Kzo9I GATC 2 cut(s) 453, 873
LmnI GCTCC 2 cut(s) 511, 680
Lsp1109I GCAGC 2 cut(s) 377, 1100
LweI GCATC 1 cut(s) 972
MaeI CTAG 1 cut(s) 939
MaeIII GTNAC 4 cut(s) 81, 376, 769, 923
MalI GATC 2 cut(s) 455, 875
MbiI CCGCTC 1 cut(s) 181
MboI GATC 2 cut(s) 453, 873
MboII GAAGA 4 cut(s) 322, 988, 1021, 1072
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 2 cut(s) 453, 873
MhlI GDGCHC 1 cut(s) 830
MluCI AATT 9 cut(s) 143, 322, 523, 542, 797, 812, 967, 972, 1103
Mly113I GGCGCC 1 cut(s) 582
MlyI GAGTC 2 cut(s) 641, 755
MmeI TCCRAC 1 cut(s) 27
MreI CGCCGGCG 1 cut(s) 22
MroI TCCGGA 1 cut(s) 121
MroNI GCCGGC 2 cut(s) 22, 190
MroXI GAANNNNTTC 1 cut(s) 1025
MseI TTAA 2 cut(s) 546, 971
MslI CAYNNNNRTG 1 cut(s) 756
MspA1I CMGCKG 2 cut(s) 481, 1035
MspI CCGG 7 cut(s) 23, 65, 122, 191, 214, 267, 928
MspR9I CCNGG 5 cut(s) 88, 214, 267, 450, 897
MunI CAATTG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 1052
MvaI CCWGG 3 cut(s) 88, 450, 897
MvnI CGCG 3 cut(s) 32, 74, 481
MwoI GCNNNNNNNGC 3 cut(s) 187, 208, 622
NaeI GCCGGC 2 cut(s) 24, 192
NarI GGCGCC 1 cut(s) 582
NciI CCSGG 2 cut(s) 214, 267
NdeII GATC 2 cut(s) 453, 873
NgoMIV GCCGGC 2 cut(s) 22, 190
NlaIII CATG 9 cut(s) 142, 202, 238, 556, 616, 704, 755, 848, 893
NlaIV GGNNCC 2 cut(s) 54, 583
NmuCI GTSAC 3 cut(s) 81, 769, 923
NotI GCGGCCGC 1 cut(s) 27
NspI RCATGY 3 cut(s) 142, 202, 848
PaeI GCATGC 1 cut(s) 202
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 234
PciI ACATGT 1 cut(s) 844
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 829
PctI GAATGC 1 cut(s) 1052
PdiI GCCGGC 2 cut(s) 24, 192
PdmI GAANNNNTTC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 244, 692, 1124
PfoI TCCNGGA 2 cut(s) 265, 895
PkrI GCNGC 5 cut(s) 28, 31, 367, 480, 1090
PleI GAGTC 2 cut(s) 640, 755
PluTI GGCGCC 1 cut(s) 585
PpsI GAGTC 2 cut(s) 640, 755
PscI ACATGT 1 cut(s) 844
Psp6I CCWGG 3 cut(s) 86, 448, 895
PspGI CCWGG 3 cut(s) 86, 448, 895
PspN4I GGNNCC 2 cut(s) 54, 583
PspXI VCTCGAGB 1 cut(s) 176
PsrI GAACNNNNNNTAC 2 cut(s) 209, 241
PstI CTGCAG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 453, 873
PvuII CAGCTG 1 cut(s) 1035
RsaI GTAC 4 cut(s) 185, 218, 257, 302
RsaNI GTAC 4 cut(s) 184, 217, 256, 301
RseI CAYNNNNRTG 1 cut(s) 756
SacII CCGCGG 1 cut(s) 482
SaqAI TTAA 2 cut(s) 546, 971
SatI GCNGC 5 cut(s) 27, 30, 366, 479, 1089
Sau3AI GATC 2 cut(s) 453, 873
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 2 cut(s) 641, 755
ScrFI CCNGG 5 cut(s) 88, 214, 267, 450, 897
SduI GDGCHC 1 cut(s) 830
SfaNI GCATC 1 cut(s) 972
SfcI CTRYAG 1 cut(s) 363
SfoI GGCGCC 1 cut(s) 583
Sfr274I CTCGAG 1 cut(s) 176
Sfr303I CCGCGG 1 cut(s) 482
SgrAI CRCCGGYG 1 cut(s) 22
SgrBI CCGCGG 1 cut(s) 482
SlaI CTCGAG 1 cut(s) 176
SmiMI CAYNNNNRTG 1 cut(s) 756
SmlI CTYRAG 3 cut(s) 176, 251, 1055
SmoI CTYRAG 3 cut(s) 176, 251, 1055
SphI GCATGC 1 cut(s) 202
Sse9I AATT 9 cut(s) 143, 322, 523, 542, 797, 812, 967, 972, 1103
SsiI CCGC 7 cut(s) 16, 26, 30, 181, 479, 481, 1128
SspDI GGCGCC 1 cut(s) 581
SspMI CTAG 1 cut(s) 939
StyD4I CCNGG 5 cut(s) 86, 212, 265, 448, 895
TaaI ACNGT 3 cut(s) 353, 935, 1121
TaqI TCGA 1 cut(s) 177
TasI AATT 9 cut(s) 143, 322, 523, 542, 797, 812, 967, 972, 1103
TatI WGTACW 2 cut(s) 255, 300
TauI GCSGC 3 cut(s) 29, 32, 481
TfiI GAWTC 3 cut(s) 244, 692, 1124
Tru1I TTAA 2 cut(s) 546, 971
Tru9I TTAA 2 cut(s) 546, 971
TseFI GTSAC 3 cut(s) 81, 769, 923
TseI GCWGC 2 cut(s) 365, 1088
Tsp45I GTSAC 3 cut(s) 81, 769, 923
TspDTI ATGAA 6 cut(s) 223, 549, 584, 740, 902, 1022
TspGWI ACGGA 1 cut(s) 982
XapI RAATTY 1 cut(s) 542
XceI RCATGY 3 cut(s) 142, 202, 848
XhoI CTCGAG 1 cut(s) 176
XmnI GAANNNNTTC 1 cut(s) 1025
XspI CTAG 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.