RLG00000005370

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007038
Physical Location & Seq
Reverse (-)
67154180 .. 67158021
3842 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005370

Sequence Viewer

Length: 1164 bp
ATGTCGGTCGACGGCCGGGACGGCCCAGCTCCGGCGGCCGGAAACGGACTGGGGAATTCGAACAATTCGGCGGCGGCGGCGGCTGCTGCGTTCGTGACGATGTCGAGCCAGCGGCTGCGGCTGAACCCTAACAAGGAGCACAAGCCGGAGAGCTACGACGACATGCAATTGGATTTCAGCCCGTCGATCTTCAGCTCTCTGGAACGGTACTTGCCGCCGAACATGCTCGGAATGCCGAGATATGATAAGGTCAAGTTCATGAGGGAGATTCTGCTCAAGTATTTGCCTCATGGAGAGCGCAACAGAGTTCAGAAGCATAGGGATTACAGACAGAAGATCATATTGAATTACCAGCCTTTGCATAAAGAGTTATACCAGCTGGATCCTGCCACCACCTTTGTTCCTGCATTTCTAGAAGCAGTTAATGAGAATACTGAGGAAAGCTTCAAAAGGATATGTTCTGAACCCTCACCTGGAATATATACTTTTGAGATTTTTCAGCCACGGTTCTGTGAATTGTTATTAGCCGAGGTGGAGAATTTTGAAAAATGGGTTGGTGACTCCAAATATAGAATCATGCGACCAAATACAATGAATAGGTATGGTGCTGTTCTTGATGATTTTGGCCTTGAAACCATGCTTGACAAGCTTTTGGAGGGTTTTGTTCGTCCTATAGCTAAAGAGCTGTTTCCTGAGGTTGGTGGATCAACTCTGGATTCTCATCATGGGTTTGTGGTTGAATATGGTAACAATAGGGATCTTGACTTGGGTTTTCATGTTGATGACTCAGAAGTAACTTTGAATGTGTGCTTGGGTAAACAATTTTCTGGAGGAGAGTTGTATTTTCGGGGAATACGTTGTGATAAACATGTAAATAGCGGAAGCCGTCCGGAGGAAGTTTTTGATTATTCTCATACCCCAGGATATGCTGTCCTTCATCGTGGTCGTCACAGGCATGGTGCTAAAGCCACGACATCAGGTCATCGTGTCAATTTACTTCTATGGTGCAGGAGTTCTGTCTTCAGAGAGATGAGGAAGTATCAGAAAGATTTCTCTAACTGGTGTGGGGAATGCTTGCATGAGAAGAAAGAGCGGCAACGGATGTCAATTACAGCTACGAAACAGGAGTTGTTGCTCAGGAAGGATGATGAGTCAACAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

388

Amino Acids

44.4

Weight (kDa)

6.93

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 113 - 235 4.3e-52 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1093
AccI GTMKAC 1 cut(s) 9
AccIII TCCGGA 1 cut(s) 889
AclWI GGATC 4 cut(s) 377, 390, 712, 765
AcoI YGGCCR 2 cut(s) 13, 36
AcsI RAATTY 2 cut(s) 55, 538
AcuI CTGAAG 2 cut(s) 175, 1006
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 6 cut(s) 31, 38, 133, 473, 698, 892
AflIII ACRYGT 1 cut(s) 868
AgsI TTSAA 6 cut(s) 346, 448, 545, 632, 740, 802
AhdI GACNNNNNGTC 1 cut(s) 978
AjnI CCWGG 2 cut(s) 472, 919
AjuI GAANNNNNNNTTGG 4 cut(s) 537, 569, 794, 826
AluBI AGCT 9 cut(s) 29, 153, 195, 379, 444, 649, 677, 685, 1115
AluI AGCT 9 cut(s) 29, 153, 195, 379, 444, 649, 677, 685, 1115
Alw21I GWGCWC 1 cut(s) 141
AlwI GGATC 4 cut(s) 377, 390, 712, 765
AlwNI CAGNNNCTG 1 cut(s) 115
Aor13HI TCCGGA 1 cut(s) 889
AoxI GGCC 4 cut(s) 13, 22, 36, 625
ApeKI GCWGC 3 cut(s) 83, 86, 115
ApoI RAATTY 2 cut(s) 55, 538
Asp700I GAANNNNTTC 1 cut(s) 1049
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 462, 569
AsuII TTCGAA 1 cut(s) 59
AxyI CCTNAGG 1 cut(s) 693
BamHI GGATCC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 1012
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 70, 73, 102
BceAI ACGGC 3 cut(s) 28, 37, 870
BciT130I CCWGG 2 cut(s) 474, 921
BcnI CCSGG 1 cut(s) 17
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 672
BglI GCCNNNNNGGC 1 cut(s) 21
Bme1390I CCNGG 3 cut(s) 17, 474, 921
BmeRI GACNNNNNGTC 1 cut(s) 978
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 1 cut(s) 384
BmrFI CCNGG 3 cut(s) 17, 474, 921
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 1012
BpmI CTGGAG 1 cut(s) 849
Bpu10I CCTNAGC 1 cut(s) 1136
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 720
BsaJI CCNNGG 3 cut(s) 503, 528, 919
BsaWI WCCGGW 1 cut(s) 889
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 38, 133, 473, 698, 892
Bse1I ACTGG 2 cut(s) 54, 1064
Bse21I CCTNAGG 1 cut(s) 693
Bse8I GATNNNNATC 1 cut(s) 720
BseAI TCCGGA 1 cut(s) 889
BseBI CCWGG 2 cut(s) 474, 921
BseDI CCNNGG 3 cut(s) 503, 528, 919
BseGI GGATG 2 cut(s) 1107, 1150
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 6 cut(s) 31, 38, 133, 473, 698, 892
BseMII CTCAG 4 cut(s) 426, 684, 801, 1150
BseNI ACTGG 2 cut(s) 54, 1064
BseRI GAGGAG 1 cut(s) 846
BseX3I CGGCCG 2 cut(s) 13, 36
BseXI GCAGC 3 cut(s) 70, 73, 102
BseYI CCCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 1027
Bsh1285I CGRYCG 3 cut(s) 9, 16, 39
BshFI GGCC 4 cut(s) 15, 24, 38, 627
BsiEI CGRYCG 3 cut(s) 9, 16, 39
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 5 cut(s) 16, 32, 39, 146, 890
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 6 cut(s) 31, 38, 133, 473, 698, 892
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 2 cut(s) 237, 1076
BsnI GGCC 4 cut(s) 15, 24, 38, 627
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 889
Bsp143I GATC 5 cut(s) 186, 336, 382, 704, 757
BspANI GGCC 4 cut(s) 15, 24, 38, 627
BspCNI CTCAG 4 cut(s) 427, 685, 800, 1149
BspEI TCCGGA 1 cut(s) 889
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 384
BspPI GGATC 4 cut(s) 377, 390, 712, 765
BspT104I TTCGAA 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 1093
BsrI ACTGG 2 cut(s) 54, 1064
BssECI CCNNGG 3 cut(s) 503, 528, 919
BssMI GATC 5 cut(s) 186, 336, 382, 704, 757
Bst2UI CCWGG 2 cut(s) 474, 921
Bst4CI ACNGT 2 cut(s) 207, 507
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 110, 1076
BstDEI CTNAG 4 cut(s) 435, 693, 787, 1136
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 2 cut(s) 1107, 1150
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 5 cut(s) 189, 339, 385, 707, 760
BstMBI GATC 5 cut(s) 186, 336, 382, 704, 757
BstMCI CGRYCG 3 cut(s) 9, 16, 39
BstNI CCWGG 2 cut(s) 474, 921
BstNSI RCATGY 3 cut(s) 166, 226, 872
BstSCI CCNGG 3 cut(s) 15, 472, 919
BstSFI CTRYAG 1 cut(s) 672
BstV1I GCAGC 3 cut(s) 70, 73, 102
BstV2I GAAGAC 1 cut(s) 1012
BstX2I RGATCY 2 cut(s) 382, 757
BstYI RGATCY 2 cut(s) 382, 757
BstZI CGGCCG 2 cut(s) 13, 36
Bsu36I CCTNAGG 1 cut(s) 693
BsuRI GGCC 4 cut(s) 15, 24, 38, 627
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 2 cut(s) 1107, 1150
Cac8I GCNNGC 2 cut(s) 110, 1076
CaiI CAGNNNCTG 1 cut(s) 115
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 208
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 4 cut(s) 435, 693, 787, 1136
DpnI GATC 5 cut(s) 188, 338, 384, 706, 759
DpnII GATC 5 cut(s) 186, 336, 382, 704, 757
DriI GACNNNNNGTC 1 cut(s) 978
EaeI YGGCCR 2 cut(s) 13, 36
EagI CGGCCG 2 cut(s) 13, 36
Eam1105I GACNNNNNGTC 1 cut(s) 978
EclXI CGGCCG 2 cut(s) 13, 36
Eco52I CGGCCG 2 cut(s) 13, 36
Eco57I CTGAAG 2 cut(s) 175, 1006
Eco81I CCTNAGG 1 cut(s) 693
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 472, 919
FaqI GGGAC 1 cut(s) 32
FblI GTMKAC 1 cut(s) 9
FokI GGATG 2 cut(s) 1114, 1157
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 299
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 849
HaeIII GGCC 4 cut(s) 15, 24, 38, 627
HapII CCGG 5 cut(s) 16, 32, 39, 146, 890
HhaI GCGC 1 cut(s) 300
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 2 cut(s) 10, 1155
HindII GTYRAC 2 cut(s) 10, 1155
HindIII AAGCTT 2 cut(s) 442, 647
HinfI GANTC 6 cut(s) 268, 560, 573, 716, 785, 1151
HpaII CCGG 5 cut(s) 16, 32, 39, 146, 890
HphI GGTGA 2 cut(s) 462, 569
Hpy166II GTNNAC 3 cut(s) 10, 818, 1155
Hpy188I TCNGA 6 cut(s) 230, 312, 463, 790, 1025, 1044
Hpy8I GTNNAC 3 cut(s) 10, 818, 1155
Hpy99I CGWCG 3 cut(s) 14, 161, 187
HpyAV CCTTC 2 cut(s) 944, 1135
HpyCH4III ACNGT 2 cut(s) 207, 507
HpyCH4IV ACGT 1 cut(s) 856
HpyCH4V TGCA 5 cut(s) 166, 361, 407, 1008, 1078
HpyF3I CTNAG 4 cut(s) 435, 693, 787, 1136
HpySE526I ACGT 1 cut(s) 856
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 1 cut(s) 889
Kzo9I GATC 5 cut(s) 186, 336, 382, 704, 757
LmnI GCTCC 2 cut(s) 34, 136
Lsp1109I GCAGC 3 cut(s) 70, 73, 102
MaeI CTAG 1 cut(s) 413
MaeII ACGT 1 cut(s) 856
MaeIII GTNAC 5 cut(s) 94, 557, 746, 793, 947
MalI GATC 5 cut(s) 188, 338, 384, 706, 759
MbiI CCGCTC 1 cut(s) 1093
MboI GATC 5 cut(s) 186, 336, 382, 704, 757
MboII GAAGA 4 cut(s) 181, 346, 1012, 1096
MfeI CAATTG 1 cut(s) 167
MflI RGATCY 2 cut(s) 382, 757
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 9 cut(s) 55, 64, 167, 346, 515, 538, 821, 991, 1107
MlyI GAGTC 3 cut(s) 554, 779, 1160
MroI TCCGGA 1 cut(s) 889
MroXI GAANNNNTTC 1 cut(s) 1049
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 2 cut(s) 780, 954
MspA1I CMGCKG 2 cut(s) 112, 379
MspI CCGG 5 cut(s) 16, 32, 39, 146, 890
MspR9I CCNGG 3 cut(s) 17, 474, 921
MunI CAATTG 1 cut(s) 167
Mva1269I GAATGC 2 cut(s) 237, 1076
MvaI CCWGG 2 cut(s) 474, 921
NciI CCSGG 1 cut(s) 17
NdeII GATC 5 cut(s) 186, 336, 382, 704, 757
NlaIV GGNNCC 1 cut(s) 384
NmeAIII GCCGAG 2 cut(s) 261, 553
NmuCI GTSAC 3 cut(s) 94, 557, 947
NspI RCATGY 3 cut(s) 166, 226, 872
NspV TTCGAA 1 cut(s) 59
PagI TCATGA 1 cut(s) 258
PciI ACATGT 1 cut(s) 868
PcsI WCGNNNNNNNCGW 1 cut(s) 853
PctI GAATGC 2 cut(s) 237, 1076
PdmI GAANNNNTTC 1 cut(s) 1049
PfeI GAWTC 3 cut(s) 268, 573, 716
PflFI GACNNNGTC 1 cut(s) 100
PleI GAGTC 3 cut(s) 554, 779, 1159
PpsI GAGTC 3 cut(s) 554, 779, 1159
PscI ACATGT 1 cut(s) 868
Psp6I CCWGG 2 cut(s) 472, 919
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 472, 919
PspN4I GGNNCC 1 cut(s) 384
PspPI GGNCC 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 2 cut(s) 382, 757
PsyI GACNNNGTC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 379
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 2 cut(s) 780, 954
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 423
Sau3AI GATC 5 cut(s) 186, 336, 382, 704, 757
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 3 cut(s) 554, 779, 1160
ScrFI CCNGG 3 cut(s) 17, 474, 921
SduI GDGCHC 1 cut(s) 141
SfcI CTRYAG 1 cut(s) 672
SfiI GGCCNNNNNGGCC 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 59
SmiMI CAYNNNNRTG 2 cut(s) 780, 954
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 9 cut(s) 55, 64, 167, 346, 515, 538, 821, 991, 1107
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 3 cut(s) 15, 472, 919
TaaI ACNGT 2 cut(s) 207, 507
TaiI ACGT 1 cut(s) 859
TaqI TCGA 4 cut(s) 9, 59, 104, 185
TasI AATT 9 cut(s) 55, 64, 167, 346, 515, 538, 821, 991, 1107
TauI GCSGC 9 cut(s) 38, 74, 77, 80, 83, 115, 121, 217, 1096
TfiI GAWTC 3 cut(s) 268, 573, 716
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseFI GTSAC 3 cut(s) 94, 557, 947
TseI GCWGC 3 cut(s) 83, 86, 115
Tsp45I GTSAC 3 cut(s) 94, 557, 947
TspDTI ATGAA 4 cut(s) 247, 608, 764, 926
TspGWI ACGGA 2 cut(s) 60, 1114
Tth111I GACNNNGTC 1 cut(s) 100
XapI RAATTY 2 cut(s) 55, 538
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 3 cut(s) 166, 226, 872
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 1049
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.