Rroxscaffold_3G00274190

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007038
Physical Location & Seq
Reverse (-)
65855713 .. 65859919
4207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274190.1

Sequence Viewer

Length: 1158 bp
ATGTCGGTCGACGGCCGGGACGGCCCAGCTCCGGCGGCCGGAAACGGACTGGGGAATTCGAACAATTCGGCTGCGGCGGCGGCGTTCGTGACGATCTCGAGCCAGCGGCTGCGGCTGAACCCTAACAAGGAGCACAAGCCGGAGAGCTACGACGACATGCAATTGGACTTCAGCCCGTCGATCTTCAGCTCTCTGGAACGGTACTTGCCGCCGAACATGCTCGGAATGCCGAGATATGATAAGGTCAAGTTCATGAGGGAGATTCTGCTCAAGTATTTGCCTCATGGAGAGCGCAACAGAGTTCAGAAGCATAGGGATTACAGACAGAAGATCATATTGAATTACCAGCCTTTGCATAAAGAGTTATACCAGCTGGATCCTGCCACCACCTTTGTTCCTGCATTTCTAGAAGCAGTTAATGAGAATACTGAGGAAAGCTTCAAAAGGATATGTTCTGAACCCTCACCTGGAATATATACCTTTGAGATTTTTCAGCCACGGTTCTGTGAATTGTTATTAGCCGAGGTGGAGAATTTTGAAAAATGGGTCGGTGACTCCAAATATAGAATCATGCGACCAAATACAATGAATAGGTATGGTGCTGTTCTTGATGATTTTGGCCTTGAAACCATGCTTGACAAGCTTTTGGAGGTTTTTGTTCGTCCTATAGCTAAAGAGCTGTTTCCTGAGGTTGGTGGATCAACTCTGGATTCTCATCATGGGTTTGTGGTTGAATATGGTAACAATAGGGATCTAGACTTGGGTTTTCATGTTGATGACTCAGAAGTAACTTTGAATGTGTGCTTGGGTAAACAATTTTCTGGAGGAGAGTTGTATTTTCGGGGAATACGTTGTGATAAACATGTAAATAGCGGAAGCCGTCCAGAGGAAGTCTTTGATTATTCTCATACCCCAGGATATGCTGTGCTTCATCGTGGTCGTCACAGGCATGGTGCTAAAGCCACGACATCAGGTCATCGTGTCAATTTACTTCTATGGTGCAGGAGTTCTGTCTTCAGAGAGATGAGGAAGTATCAGAAAGATTTCTCTAACTGGTGTGGGGAATGCTTGCATGAGAAGAAAGAGAGGCAACGGATGTCAATTACAGCTACAAAACAGGAGTTGTTGCTCAGGAAGGATGATGAGTCAACAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

385

Amino Acids

44.28

Weight (kDa)

6.93

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 111 - 233 6.6e-52 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AciI CCGC 8 cut(s) 35, 74, 77, 80, 106, 112, 209, 873
AclWI GGATC 4 cut(s) 371, 384, 706, 759
AcoI YGGCCR 2 cut(s) 13, 36
AcsI RAATTY 2 cut(s) 55, 532
AcuI CTGAAG 3 cut(s) 154, 169, 1000
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 6 cut(s) 31, 38, 127, 467, 692, 886
AflIII ACRYGT 1 cut(s) 862
AgsI TTSAA 6 cut(s) 340, 442, 539, 626, 734, 796
AhdI GACNNNNNGTC 1 cut(s) 972
AjnI CCWGG 2 cut(s) 466, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
AluBI AGCT 9 cut(s) 29, 147, 189, 373, 438, 643, 671, 679, 1109
AluI AGCT 9 cut(s) 29, 147, 189, 373, 438, 643, 671, 679, 1109
Alw21I GWGCWC 1 cut(s) 135
AlwI GGATC 4 cut(s) 371, 384, 706, 759
AlwNI CAGNNNCTG 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 97
AoxI GGCC 4 cut(s) 13, 22, 36, 619
ApeKI GCWGC 2 cut(s) 71, 109
ApoI RAATTY 2 cut(s) 55, 532
Asp700I GAANNNNTTC 1 cut(s) 1043
AspLEI GCGC 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 2 cut(s) 456, 563
AsuII TTCGAA 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 97
AxyI CCTNAGG 1 cut(s) 687
BamHI GGATCC 1 cut(s) 376
BbsI GAAGAC 1 cut(s) 1006
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 58, 96
BceAI ACGGC 3 cut(s) 28, 37, 864
BciT130I CCWGG 2 cut(s) 468, 915
BcnI CCSGG 1 cut(s) 17
BfaI CTAG 2 cut(s) 407, 755
BfmI CTRYAG 1 cut(s) 666
BglI GCCNNNNNGGC 1 cut(s) 21
BisI GCNGC 9 cut(s) 36, 72, 75, 78, 81, 107, 110, 113, 209
BlsI GCNGC 9 cut(s) 37, 73, 76, 79, 82, 108, 111, 114, 210
Bme1390I CCNGG 3 cut(s) 17, 468, 915
BmeRI GACNNNNNGTC 1 cut(s) 972
BmeT110I CYCGRG 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 3 cut(s) 17, 468, 915
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 1006
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 1130
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 714
BsaJI CCNNGG 3 cut(s) 497, 522, 913
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 38, 127, 467, 692, 886
Bse1I ACTGG 2 cut(s) 54, 1058
Bse21I CCTNAGG 1 cut(s) 687
Bse8I GATNNNNATC 1 cut(s) 714
BseBI CCWGG 2 cut(s) 468, 915
BseDI CCNNGG 3 cut(s) 497, 522, 913
BseGI GGATG 2 cut(s) 1101, 1144
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 6 cut(s) 31, 38, 127, 467, 692, 886
BseMII CTCAG 4 cut(s) 420, 678, 795, 1144
BseNI ACTGG 2 cut(s) 54, 1058
BseRI GAGGAG 1 cut(s) 840
BseX3I CGGCCG 2 cut(s) 13, 36
BseXI GCAGC 2 cut(s) 58, 96
BseYI CCCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 1021
Bsh1285I CGRYCG 3 cut(s) 9, 16, 39
BshFI GGCC 4 cut(s) 15, 24, 38, 621
BsiEI CGRYCG 3 cut(s) 9, 16, 39
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 4 cut(s) 16, 32, 39, 140
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 6 cut(s) 31, 38, 127, 467, 692, 886
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 2 cut(s) 231, 1070
BsnI GGCC 4 cut(s) 15, 24, 38, 621
BsoBI CYCGRG 1 cut(s) 97
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 6 cut(s) 93, 180, 330, 376, 698, 751
BspACI CCGC 8 cut(s) 35, 74, 77, 80, 106, 112, 209, 873
BspANI GGCC 4 cut(s) 15, 24, 38, 621
BspCNI CTCAG 4 cut(s) 421, 679, 794, 1143
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 378
BspPI GGATC 4 cut(s) 371, 384, 706, 759
BspT104I TTCGAA 1 cut(s) 59
BsrI ACTGG 2 cut(s) 54, 1058
BssECI CCNNGG 3 cut(s) 497, 522, 913
BssMI GATC 6 cut(s) 93, 180, 330, 376, 698, 751
Bst2UI CCWGG 2 cut(s) 468, 915
Bst4CI ACNGT 2 cut(s) 201, 501
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 104, 1070
BstDEI CTNAG 4 cut(s) 429, 687, 781, 1130
BstDSI CCRYGG 1 cut(s) 497
BstF5I GGATG 2 cut(s) 1101, 1144
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 6 cut(s) 96, 183, 333, 379, 701, 754
BstMBI GATC 6 cut(s) 93, 180, 330, 376, 698, 751
BstMCI CGRYCG 3 cut(s) 9, 16, 39
BstMWI GCNNNNNNNGC 8 cut(s) 21, 35, 77, 80, 112, 217, 226, 640
BstNI CCWGG 2 cut(s) 468, 915
BstNSI RCATGY 3 cut(s) 160, 220, 866
BstSCI CCNGG 3 cut(s) 15, 466, 913
BstSFI CTRYAG 1 cut(s) 666
BstV1I GCAGC 2 cut(s) 58, 96
BstV2I GAAGAC 1 cut(s) 1006
BstX2I RGATCY 2 cut(s) 376, 751
BstYI RGATCY 2 cut(s) 376, 751
BstZI CGGCCG 2 cut(s) 13, 36
Bsu36I CCTNAGG 1 cut(s) 687
BsuRI GGCC 4 cut(s) 15, 24, 38, 621
BtgI CCRYGG 1 cut(s) 497
BtsCI GGATG 2 cut(s) 1101, 1144
Cac8I GCNNGC 2 cut(s) 104, 1070
CaiI CAGNNNCTG 1 cut(s) 109
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 202
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 4 cut(s) 429, 687, 781, 1130
DpnI GATC 6 cut(s) 95, 182, 332, 378, 700, 753
DpnII GATC 6 cut(s) 93, 180, 330, 376, 698, 751
DriI GACNNNNNGTC 1 cut(s) 972
EaeI YGGCCR 2 cut(s) 13, 36
EagI CGGCCG 2 cut(s) 13, 36
Eam1105I GACNNNNNGTC 1 cut(s) 972
EclXI CGGCCG 2 cut(s) 13, 36
Eco52I CGGCCG 2 cut(s) 13, 36
Eco57I CTGAAG 3 cut(s) 154, 169, 1000
Eco81I CCTNAGG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 466, 913
FaqI GGGAC 1 cut(s) 32
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 9 cut(s) 36, 72, 75, 78, 81, 107, 110, 113, 209
FokI GGATG 2 cut(s) 1108, 1151
Fsp4HI GCNGC 9 cut(s) 36, 72, 75, 78, 81, 107, 110, 113, 209
FspBI CTAG 2 cut(s) 407, 755
GlaI GCGC 1 cut(s) 293
GluI GCNGC 9 cut(s) 36, 72, 75, 78, 81, 107, 110, 113, 209
GsaI CCCAGC 1 cut(s) 29
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 4 cut(s) 15, 24, 38, 621
HapII CCGG 4 cut(s) 16, 32, 39, 140
HhaI GCGC 1 cut(s) 294
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 2 cut(s) 10, 1149
HindII GTYRAC 2 cut(s) 10, 1149
HindIII AAGCTT 2 cut(s) 436, 641
HinfI GANTC 6 cut(s) 262, 554, 567, 710, 779, 1145
HpaII CCGG 4 cut(s) 16, 32, 39, 140
HphI GGTGA 2 cut(s) 456, 563
Hpy166II GTNNAC 3 cut(s) 10, 812, 1149
Hpy188I TCNGA 6 cut(s) 224, 306, 457, 784, 1019, 1038
Hpy8I GTNNAC 3 cut(s) 10, 812, 1149
Hpy99I CGWCG 3 cut(s) 14, 155, 181
HpyAV CCTTC 1 cut(s) 1129
HpyCH4III ACNGT 2 cut(s) 201, 501
HpyCH4IV ACGT 1 cut(s) 850
HpyCH4V TGCA 5 cut(s) 160, 355, 401, 1002, 1072
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 35, 77, 80, 112, 217, 226, 640
HpyF3I CTNAG 4 cut(s) 429, 687, 781, 1130
HpySE526I ACGT 1 cut(s) 850
HspAI GCGC 1 cut(s) 292
Kzo9I GATC 6 cut(s) 93, 180, 330, 376, 698, 751
LmnI GCTCC 2 cut(s) 34, 130
Lsp1109I GCAGC 2 cut(s) 58, 96
MaeI CTAG 2 cut(s) 407, 755
MaeII ACGT 1 cut(s) 850
MaeIII GTNAC 5 cut(s) 88, 551, 740, 787, 941
MalI GATC 6 cut(s) 95, 182, 332, 378, 700, 753
MboI GATC 6 cut(s) 93, 180, 330, 376, 698, 751
MboII GAAGA 4 cut(s) 175, 340, 1006, 1090
MfeI CAATTG 1 cut(s) 161
MflI RGATCY 2 cut(s) 376, 751
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 9 cut(s) 55, 64, 161, 340, 509, 532, 815, 985, 1101
MlyI GAGTC 3 cut(s) 548, 773, 1154
MroXI GAANNNNTTC 1 cut(s) 1043
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 2 cut(s) 774, 948
MspA1I CMGCKG 2 cut(s) 106, 373
MspI CCGG 4 cut(s) 16, 32, 39, 140
MspR9I CCNGG 3 cut(s) 17, 468, 915
MunI CAATTG 1 cut(s) 161
Mva1269I GAATGC 2 cut(s) 231, 1070
MvaI CCWGG 2 cut(s) 468, 915
MwoI GCNNNNNNNGC 8 cut(s) 21, 35, 77, 80, 112, 217, 226, 640
NciI CCSGG 1 cut(s) 17
NdeII GATC 6 cut(s) 93, 180, 330, 376, 698, 751
NlaIV GGNNCC 1 cut(s) 378
NmeAIII GCCGAG 2 cut(s) 255, 547
NmuCI GTSAC 3 cut(s) 88, 551, 941
NspI RCATGY 3 cut(s) 160, 220, 866
NspV TTCGAA 1 cut(s) 59
PaeR7I CTCGAG 1 cut(s) 97
PagI TCATGA 1 cut(s) 252
PciI ACATGT 1 cut(s) 862
PcsI WCGNNNNNNNCGW 1 cut(s) 847
PctI GAATGC 2 cut(s) 231, 1070
PdmI GAANNNNTTC 1 cut(s) 1043
PfeI GAWTC 3 cut(s) 262, 567, 710
PkrI GCNGC 9 cut(s) 37, 73, 76, 79, 82, 108, 111, 114, 210
PleI GAGTC 3 cut(s) 548, 773, 1153
PpsI GAGTC 3 cut(s) 548, 773, 1153
PscI ACATGT 1 cut(s) 862
Psp6I CCWGG 2 cut(s) 466, 913
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 466, 913
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 2 cut(s) 376, 751
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 2 cut(s) 774, 948
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 9 cut(s) 36, 72, 75, 78, 81, 107, 110, 113, 209
Sau3AI GATC 6 cut(s) 93, 180, 330, 376, 698, 751
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 3 cut(s) 548, 773, 1154
ScrFI CCNGG 3 cut(s) 17, 468, 915
SduI GDGCHC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 666
SfiI GGCCNNNNNGGCC 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 97
SfuI TTCGAA 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 97
SmiMI CAYNNNNRTG 2 cut(s) 774, 948
SmlI CTYRAG 2 cut(s) 97, 269
SmoI CTYRAG 2 cut(s) 97, 269
Sse9I AATT 9 cut(s) 55, 64, 161, 340, 509, 532, 815, 985, 1101
SsiI CCGC 8 cut(s) 35, 74, 77, 80, 106, 112, 209, 873
SspMI CTAG 2 cut(s) 407, 755
StyD4I CCNGG 3 cut(s) 15, 466, 913
TaaI ACNGT 2 cut(s) 201, 501
TaiI ACGT 1 cut(s) 853
TaqI TCGA 4 cut(s) 9, 59, 98, 179
TasI AATT 9 cut(s) 55, 64, 161, 340, 509, 532, 815, 985, 1101
TauI GCSGC 7 cut(s) 38, 77, 80, 83, 109, 115, 211
TfiI GAWTC 3 cut(s) 262, 567, 710
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseFI GTSAC 3 cut(s) 88, 551, 941
TseI GCWGC 2 cut(s) 71, 109
Tsp45I GTSAC 3 cut(s) 88, 551, 941
TspDTI ATGAA 4 cut(s) 241, 602, 758, 920
TspGWI ACGGA 2 cut(s) 60, 1108
XapI RAATTY 2 cut(s) 55, 532
XbaI TCTAGA 2 cut(s) 406, 754
XceI RCATGY 3 cut(s) 160, 220, 866
XhoI CTCGAG 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 1043
XspI CTAG 2 cut(s) 407, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.