Rh7DG022400

PKHD-type hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007038
Physical Location & Seq
Forward (+)
1763953 .. 1768335
4383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG022400.1

Sequence Viewer

Length: 813 bp
ATGTCGGTCGACGGCCGGGACGGCCCAGCTCCGGCGCCCGGAAACGGACTGGGGAATTCGAACAATTCGGCGGCGGCGTTCGTGACGATGTCGAGCCAGCGGCTGCGGCTGAACCCTAACAAGGAGCACAAGCCGGAGAGCTACGAGGACATGCAATTGGATTTCAGCCCGTCGATCTTCAGCTCTCTGGAACGGTACTTGCCGCCGAACATGCTCGGAATGCCGAGATATGATAAGGTCAAGTTCATGAGGGAGATTCTGCTCAAGTATTTGCCTCATGGAGAGCGCAACAGGGTTCAGAAGCATAGGGATTACAGACAGAAGATCATATTGAATTACCAGCCTTTGCATAAAGAGTTATACCTGCTGGATCCTGCCACCACCTTTGTTCCTGCATTTCTAGAAGCAGTTAATGAGAATACTGAGGAAAGCTTCAAAAGGATATGTTCTGAACCCTCACCTGGAATATATACTTTTGAGATTTTTCAGCCACGGTTCTGTGAATTGTTATTAGCCGAGGTGGAGAATTTTGAAAAATGGGTCGGTGACTCCAAATATAGAATCATGCGACCAAATACAATGAATAGGTATGGTGCGGTTCTTGATGATTTTGGCCTTGAAACCATGCTTGACAAGCTTTTGGAGGGTTTTGTTCGCCCTATAGCTAAAGAGCTGTTTCCTGAGGTTGGTGGATCAACTCTGGATTCTCATCATGGGTTTGTGGTTGAATATGGTAACAATAGGGATCTTGACTTGGGTAAGTATTTTGTTTTATGGATATATAGTTTGAAGTTTATCCTTTTTTGCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

31.21

Weight (kDa)

5.93

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OGFOD2-like PF25238 109 - 231 9e-52 OGFOD2-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 34
AccI GTMKAC 1 cut(s) 9
AciI CCGC 6 cut(s) 71, 74, 100, 106, 203, 596
AclWI GGATC 4 cut(s) 365, 378, 700, 753
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 2 cut(s) 55, 526
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 6 cut(s) 31, 38, 44, 121, 461, 686
AgsI TTSAA 6 cut(s) 334, 436, 533, 620, 728, 790
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 7 cut(s) 29, 141, 183, 432, 637, 665, 673
AluI AGCT 7 cut(s) 29, 141, 183, 432, 637, 665, 673
Alw21I GWGCWC 1 cut(s) 129
AlwI GGATC 4 cut(s) 365, 378, 700, 753
AlwNI CAGNNNCTG 1 cut(s) 103
AoxI GGCC 3 cut(s) 13, 22, 613
ApeKI GCWGC 1 cut(s) 103
ApoI RAATTY 2 cut(s) 55, 526
AspLEI GCGC 2 cut(s) 37, 288
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 2 cut(s) 17, 39
AsuHPI GGTGA 2 cut(s) 450, 557
AsuII TTCGAA 1 cut(s) 59
AxyI CCTNAGG 1 cut(s) 681
BamHI GGATCC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 90
BceAI ACGGC 2 cut(s) 28, 37
BciT130I CCWGG 1 cut(s) 462
BcnI CCSGG 2 cut(s) 17, 39
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 38
BfuAI ACCTGC 1 cut(s) 372
BglI GCCNNNNNGGC 1 cut(s) 21
BisI GCNGC 6 cut(s) 72, 75, 101, 104, 107, 203
BlsI GCNGC 6 cut(s) 73, 76, 102, 105, 108, 204
Bme1390I CCNGG 3 cut(s) 17, 39, 462
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 2 cut(s) 36, 372
BmrFI CCNGG 3 cut(s) 17, 39, 462
BmrI ACTGGG 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 59
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 17, 39
BsaBI GATNNNNATC 1 cut(s) 708
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 491, 516
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 38, 44, 121, 461, 686
Bse1I ACTGG 1 cut(s) 54
Bse21I CCTNAGG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 708
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 491, 516
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 6 cut(s) 31, 38, 44, 121, 461, 686
BseMII CTCAG 2 cut(s) 414, 672
BseNI ACTGG 1 cut(s) 54
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 90
BseYI CCCAGC 1 cut(s) 25
Bsh1285I CGRYCG 2 cut(s) 9, 16
BshFI GGCC 3 cut(s) 15, 24, 615
BshNI GGYRCC 1 cut(s) 34
BsiEI CGRYCG 2 cut(s) 9, 16
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 4 cut(s) 16, 32, 39, 134
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 6 cut(s) 31, 38, 44, 121, 461, 686
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 225
BsnI GGCC 3 cut(s) 15, 24, 615
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 5 cut(s) 174, 324, 370, 692, 745
BspACI CCGC 6 cut(s) 71, 74, 100, 106, 203, 596
BspANI GGCC 3 cut(s) 15, 24, 615
BspCNI CTCAG 2 cut(s) 415, 673
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 36, 372
BspMI ACCTGC 1 cut(s) 372
BspPI GGATC 4 cut(s) 365, 378, 700, 753
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 34
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 2 cut(s) 491, 516
BssMI GATC 5 cut(s) 174, 324, 370, 692, 745
BssNI GRCGYC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 195, 495
BstACI GRCGYC 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 98
BstDEI CTNAG 2 cut(s) 423, 681
BstDSI CCRYGG 1 cut(s) 491
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 2 cut(s) 37, 288
BstKTI GATC 5 cut(s) 177, 327, 373, 695, 748
BstMBI GATC 5 cut(s) 174, 324, 370, 692, 745
BstMCI CGRYCG 2 cut(s) 9, 16
BstMWI GCNNNNNNNGC 5 cut(s) 21, 106, 211, 220, 634
BstNI CCWGG 1 cut(s) 462
BstNSI RCATGY 2 cut(s) 154, 214
BstSCI CCNGG 3 cut(s) 15, 37, 460
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 1 cut(s) 90
BstX2I RGATCY 2 cut(s) 370, 745
BstYI RGATCY 2 cut(s) 370, 745
BstZI CGGCCG 1 cut(s) 13
Bsu36I CCTNAGG 1 cut(s) 681
BsuRI GGCC 3 cut(s) 15, 24, 615
BtgI CCRYGG 1 cut(s) 491
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 98
CaiI CAGNNNCTG 1 cut(s) 103
CciI TCATGA 1 cut(s) 246
CfoI GCGC 2 cut(s) 37, 288
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 196
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 7 cut(s) 151, 211, 247, 278, 565, 625, 713
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 423, 681
DinI GGCGCC 1 cut(s) 36
DpnI GATC 5 cut(s) 176, 326, 372, 694, 747
DpnII GATC 5 cut(s) 174, 324, 370, 692, 745
EaeI YGGCCR 1 cut(s) 13
EagI CGGCCG 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 13
Eco52I CGGCCG 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 163
Eco81I CCTNAGG 1 cut(s) 681
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 460
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
FaeI CATG 7 cut(s) 154, 214, 250, 281, 568, 628, 716
FaqI GGGAC 1 cut(s) 32
FatI CATG 7 cut(s) 150, 210, 246, 277, 564, 624, 712
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 6 cut(s) 72, 75, 101, 104, 107, 203
Fsp4HI GCNGC 6 cut(s) 72, 75, 101, 104, 107, 203
FspBI CTAG 1 cut(s) 401
GlaI GCGC 2 cut(s) 36, 287
GluI GCNGC 6 cut(s) 72, 75, 101, 104, 107, 203
GsaI CCCAGC 1 cut(s) 29
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 3 cut(s) 15, 24, 615
HapII CCGG 4 cut(s) 16, 32, 39, 134
HhaI GCGC 2 cut(s) 37, 288
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 7 cut(s) 154, 214, 250, 281, 568, 628, 716
Hin6I GCGC 2 cut(s) 35, 286
HinP1I GCGC 2 cut(s) 35, 286
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 2 cut(s) 430, 635
HinfI GANTC 4 cut(s) 256, 548, 561, 704
HpaII CCGG 4 cut(s) 16, 32, 39, 134
HphI GGTGA 2 cut(s) 450, 557
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 3 cut(s) 218, 300, 451
Hpy188III TCNNGA 8 cut(s) 82, 188, 247, 401, 602, 680, 701, 749
Hpy8I GTNNAC 1 cut(s) 10
Hpy99I CGWCG 2 cut(s) 14, 175
HpyCH4III ACNGT 2 cut(s) 195, 495
HpyCH4V TGCA 4 cut(s) 154, 349, 395, 807
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 106, 211, 220, 634
HpyF3I CTNAG 2 cut(s) 423, 681
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 7 cut(s) 154, 214, 250, 281, 568, 628, 716
HspAI GCGC 2 cut(s) 35, 286
KasI GGCGCC 1 cut(s) 34
Kzo9I GATC 5 cut(s) 174, 324, 370, 692, 745
LmnI GCTCC 2 cut(s) 34, 124
Lsp1109I GCAGC 1 cut(s) 90
MaeI CTAG 1 cut(s) 401
MaeIII GTNAC 3 cut(s) 82, 545, 734
MalI GATC 5 cut(s) 176, 326, 372, 694, 747
MboI GATC 5 cut(s) 174, 324, 370, 692, 745
MboII GAAGA 2 cut(s) 169, 334
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 2 cut(s) 370, 745
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 6 cut(s) 55, 64, 155, 334, 503, 526
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 8 cut(s) 139, 243, 285, 418, 466, 511, 637, 676
MseI TTAA 2 cut(s) 411, 811
MspA1I CMGCKG 1 cut(s) 100
MspI CCGG 4 cut(s) 16, 32, 39, 134
MspR9I CCNGG 3 cut(s) 17, 39, 462
MunI CAATTG 1 cut(s) 155
Mva1269I GAATGC 1 cut(s) 225
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 5 cut(s) 21, 106, 211, 220, 634
NarI GGCGCC 1 cut(s) 35
NciI CCSGG 2 cut(s) 17, 39
NdeII GATC 5 cut(s) 174, 324, 370, 692, 745
NlaIII CATG 7 cut(s) 154, 214, 250, 281, 568, 628, 716
NlaIV GGNNCC 2 cut(s) 36, 372
NmeAIII GCCGAG 2 cut(s) 249, 541
NmuCI GTSAC 2 cut(s) 82, 545
NspI RCATGY 2 cut(s) 154, 214
NspV TTCGAA 1 cut(s) 59
PagI TCATGA 1 cut(s) 246
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 1 cut(s) 225
PfeI GAWTC 3 cut(s) 256, 561, 704
PflFI GACNNNGTC 1 cut(s) 88
PkrI GCNGC 6 cut(s) 73, 76, 102, 105, 108, 204
PleI GAGTC 1 cut(s) 542
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 460
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 2 cut(s) 36, 372
PspPI GGNCC 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 103
PsuI RGATCY 2 cut(s) 370, 745
PsyI GACNNNGTC 1 cut(s) 88
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 411, 811
SatI GCNGC 6 cut(s) 72, 75, 101, 104, 107, 203
Sau3AI GATC 5 cut(s) 174, 324, 370, 692, 745
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 3 cut(s) 17, 39, 462
SduI GDGCHC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 660
SfiI GGCCNNNNNGGCC 1 cut(s) 21
SfoI GGCGCC 1 cut(s) 36
SfuI TTCGAA 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 6 cut(s) 55, 64, 155, 334, 503, 526
SsiI CCGC 6 cut(s) 71, 74, 100, 106, 203, 596
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 3 cut(s) 15, 37, 460
TaaI ACNGT 2 cut(s) 195, 495
TaqI TCGA 4 cut(s) 9, 59, 92, 173
TasI AATT 6 cut(s) 55, 64, 155, 334, 503, 526
TauI GCSGC 5 cut(s) 74, 77, 103, 109, 205
TfiI GAWTC 3 cut(s) 256, 561, 704
Tru1I TTAA 2 cut(s) 411, 811
Tru9I TTAA 2 cut(s) 411, 811
TseFI GTSAC 2 cut(s) 82, 545
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 2 cut(s) 82, 545
TspDTI ATGAA 2 cut(s) 235, 596
TspGWI ACGGA 1 cut(s) 60
Tth111I GACNNNGTC 1 cut(s) 88
XapI RAATTY 2 cut(s) 55, 526
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 2 cut(s) 154, 214
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.