FvH4_5g19490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015400
Physical Location & Seq
Reverse (-)
11330278 .. 11333127
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19490.t1

Sequence Viewer

Length: 1464 bp
ATGAACCTCCAAACCCTAAAACCCACCTTCCTCCGATCACAGCACCCCAGAAAGCACCCTTCCTCCAAAAGATCCCCACTCCTCTACGTCACCCTCATCTCCTCCCTCCTCCTCTTCCTCCTTTACTTCTTCTCCCTCAACCCCACCTCCCTCCTCCTCTCCAATTCCCCAAAATGCCCTTCTCACAGGTTCCTCTGGTACGCCCCCCACAGCGGGTTCAGCAACCAGCTCATGGAGCTCAAGAACGGAATCCTCATGGCCGGCATTCTCAACCGGACGCTAATTGTGCCGCCGGTGCTGGATCACCACGCCGTGGCTCTCGGGAGTTGCCCCAAGTTTCGGGTTTCGGCTCCGAATGAGATCAGGGGGCAGGTGTGGGAGCATGTGGTTGAGCTCATAAGGAGTGGGAGGTATGTATCCATGGCTGACATTGTTGATCTATCGTCGTTAGTTTCTTCTTCATTAGTTCGGGTTATAGATTTCAGGGATTTTATGTCCTTGTGGTGCGATGTGAACTTAGATTTTGCTTGCCCTAATGAGTTCAATGCACAGCCTCATTTGCTGGATAAACTGAAGGAATGTGGATCAGTACTAACTGGGGTGAAAGGTAATGTTAAGTGTTTACATGCTGTTAATGAAGACTGCAGAACCACTGTTTGGACATACCAGAATGGTAATGAGGATGGGGCATTAGATTCTTTTCAACCCGATGAAAAGCTCAAGAAAAAGAAAATTTCATATGTTAGGAAACGACGAGATGTATATAAGACTCTTGGACCCGGTTCTGAATCTGAATCAGCTTCTGTGCTGGCATTTGGAAGCCTTTTCACTGCTCCTTACAAAGGTTCCGAGTTACTCATTGATATCCGTGAATCTCCAAGAGATCAAAGGATAGGTACTCTATTGGAGAAGATTGAATTTCTTCCCTTTGCCCCTGAAATTACGAGTGCAGGAAAGAAGTTTGCTTTGGAAACCATAAAGGCTCCTTTTCTTTGCGCACAGCTTAGATTATTAGATGGGCAGTTCAAGAATCATTGGAAAACTACTTTTCAAGGGTTAAAACAAAAGCTAGATGCTTTGACGCAGAGCCCTCTACCCATTCATATCTTTGTGATGACTGATCTTCCTGAAACTAATTGGACTGGAAATTACTTGGGGGATTTGTCTAGAGATCCTAGTCAGTTTAAACTGTTTTTCCTGAAAGAAAGTGATGAGGTGGTAATACAAACGGCTAAACGAATTGTTGATGCAGACCATAGCTTGAAGTTTGGGTCTTCTGCAAATATTCATGATGGAACTGATCAGATTAAGAAGGATTGTCCTTCTGAAGTAATACCCGACGTGCTTCTATACATAGAGCAAACTGTATGCAGTTGTGCTTCTCTTGGATTTGTTGGGACTGCAGGATCAACTATTGCAGGGAGTATAGAGCTAATGAGAAAGTTCCGTGCATGTTCTAGCTAA

Protein Analysis

488

Amino Acids

54.46

Weight (kDa)

8.6

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17430
fragaria_vesca FvH4_5g19490
malus_domestica MD04G1060600.v1.1
prunus_persica Prupe.5G065000_v2.0.a1
pyrus_communis pycom04g05440 pycom06g04540
rosa_chinensis RchiOBHm_Chr7g0204071
rosa_laevigata RLG00000003490
rosa_multiflora Rmu_co8337455.1_g000001
rosa_roxburghii Rroxscaffold_3G00253380
rosa_rugosa Rorug07G0080400
rosa_samantha Rh7AG210400 Rh7BG209000 Rh7CG222800 Rh7DG217100
rosa_wichuraiana Rw7G018320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 361
Acc16I TGCGCA 1 cut(s) 997
Acc36I ACCTGC 1 cut(s) 361
AccB7I CCANNNNNTGG 3 cut(s) 232, 313, 657
AciI CCGC 2 cut(s) 213, 290
AclWI GGATC 5 cut(s) 66, 309, 592, 1166, 1414
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 2 cut(s) 732, 917
AcuI CTGAAG 2 cut(s) 593, 1347
AdeI CACNNNGTG 1 cut(s) 313
AfaI GTAC 3 cut(s) 200, 591, 898
AfiI CCNNNNNNNGG 7 cut(s) 212, 213, 232, 313, 339, 657, 842
AgsI TTSAA 6 cut(s) 544, 704, 917, 1027, 1052, 1264
AjiI CACGTC 1 cut(s) 1342
AjuI GAANNNNNNNTTGG 6 cut(s) 163, 195, 640, 672, 950, 982
Alw21I GWGCWC 2 cut(s) 240, 396
AlwI GGATC 5 cut(s) 66, 309, 592, 1166, 1414
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 1 cut(s) 258
ApoI RAATTY 2 cut(s) 732, 917
Asp700I GAANNNNTTC 1 cut(s) 921
AspLEI GCGC 1 cut(s) 998
AspS9I GGNCC 1 cut(s) 776
AsuC2I CCSGG 1 cut(s) 780
AsuHPI GGTGA 3 cut(s) 82, 296, 613
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 1 cut(s) 776
BanII GRGCYC 3 cut(s) 240, 396, 1091
BbsI GAAGAC 2 cut(s) 645, 1266
Bbv12I GWGCWC 2 cut(s) 240, 396
BccI CCATC 3 cut(s) 677, 1010, 1286
BceAI ACGGC 2 cut(s) 296, 1245
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 1300
BcnI CCSGG 1 cut(s) 780
BfaI CTAG 4 cut(s) 1070, 1167, 1176, 1458
BfmI CTRYAG 2 cut(s) 643, 1401
BfuAI ACCTGC 1 cut(s) 361
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
BmcAI AGTACT 1 cut(s) 591
Bme1390I CCNGG 1 cut(s) 780
Bme18I GGWCC 1 cut(s) 776
BmeT110I CYCGRG 1 cut(s) 320
BmgBI CACGTC 1 cut(s) 1342
BmgT120I GGNCC 1 cut(s) 776
BmiI GGNNCC 5 cut(s) 191, 351, 778, 847, 984
BmrFI CCNGG 1 cut(s) 780
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 2 cut(s) 1063, 1237
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 2 cut(s) 645, 1266
BpuEI CTTGAG 2 cut(s) 224, 704
BpuMI CCSGG 1 cut(s) 780
BsaJI CCNNGG 2 cut(s) 312, 420
BsaWI WCCGGW 1 cut(s) 273
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 116, 131, 146, 161
Bsc4I CCNNNNNNNGG 7 cut(s) 212, 213, 232, 313, 339, 657, 842
Bse118I RCCGGY 2 cut(s) 260, 292
Bse1I ACTGG 2 cut(s) 601, 1147
BseDI CCNNGG 2 cut(s) 312, 420
BseGI GGATG 1 cut(s) 688
BseLI CCNNNNNNNGG 7 cut(s) 212, 213, 232, 313, 339, 657, 842
BseNI ACTGG 2 cut(s) 601, 1147
BseRI GAGGAG 6 cut(s) 71, 91, 98, 101, 143, 146
BsgI GTGCAG 1 cut(s) 969
BshFI GGCC 1 cut(s) 260
BsiHKAI GWGCWC 2 cut(s) 240, 396
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 4 cut(s) 261, 274, 293, 780
BslFI GGGAC 1 cut(s) 1411
BslI CCNNNNNNNGG 7 cut(s) 212, 213, 232, 313, 339, 657, 842
BsmFI GGGAC 1 cut(s) 1411
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 3 cut(s) 240, 396, 1091
Bsp19I CCATGG 1 cut(s) 420
BspACI CCGC 2 cut(s) 213, 290
BspANI GGCC 1 cut(s) 260
BspHI TCATGA 1 cut(s) 1288
BspLI GGNNCC 5 cut(s) 191, 351, 778, 847, 984
BspMAI CTGCAG 2 cut(s) 647, 1405
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 5 cut(s) 66, 309, 592, 1166, 1414
BsrFI RCCGGY 2 cut(s) 260, 292
BsrI ACTGG 2 cut(s) 601, 1147
BssAI RCCGGY 2 cut(s) 260, 292
BssECI CCNNGG 2 cut(s) 312, 420
BssT1I CCWWGG 1 cut(s) 420
Bst4CI ACNGT 3 cut(s) 655, 1191, 1366
Bst6I CTCTTC 1 cut(s) 119
BstC8I GCNNGC 3 cut(s) 262, 529, 810
BstDEI CTNAG 2 cut(s) 517, 1004
BstDSI CCRYGG 2 cut(s) 312, 420
BstENI CCTNNNNNAGG 1 cut(s) 840
BstF5I GGATG 1 cut(s) 688
BstHHI GCGC 1 cut(s) 998
BstMWI GCNNNNNNNGC 5 cut(s) 219, 235, 286, 295, 559
BstNSI RCATGY 3 cut(s) 386, 629, 1455
BstSCI CCNGG 1 cut(s) 778
BstSFI CTRYAG 2 cut(s) 643, 1401
BstV2I GAAGAC 2 cut(s) 645, 1266
BstX2I RGATCY 2 cut(s) 71, 1171
BstYI RGATCY 2 cut(s) 71, 1171
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 1 cut(s) 260
BtgI CCRYGG 2 cut(s) 312, 420
BtgZI GCGATG 1 cut(s) 522
BtrI CACGTC 1 cut(s) 1342
BtsCI GGATG 1 cut(s) 688
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 651, 828
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 3 cut(s) 262, 529, 810
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 1 cut(s) 1288
CfoI GCGC 1 cut(s) 998
Cfr10I RCCGGY 2 cut(s) 260, 292
Cfr13I GGNCC 1 cut(s) 776
CseI GACGC 2 cut(s) 286, 1090
Csp6I GTAC 3 cut(s) 199, 590, 897
CviAII CATG 7 cut(s) 232, 256, 383, 421, 626, 1289, 1452
CviQI GTAC 3 cut(s) 199, 590, 897
DdeI CTNAG 2 cut(s) 517, 1004
DraI TTTAAA 1 cut(s) 1186
DraIII CACNNNGTG 1 cut(s) 313
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Ecl136II GAGCTC 2 cut(s) 238, 394
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 3 cut(s) 240, 396, 1091
Eco32I GATATC 1 cut(s) 865
Eco47I GGWCC 1 cut(s) 776
Eco53kI GAGCTC 2 cut(s) 238, 394
Eco57I CTGAAG 2 cut(s) 593, 1347
Eco88I CYCGRG 1 cut(s) 320
EcoICRI GAGCTC 2 cut(s) 238, 394
EcoNI CCTNNNNNAGG 1 cut(s) 840
EcoRV GATATC 1 cut(s) 865
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 3 cut(s) 240, 396, 1091
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 7 cut(s) 235, 259, 386, 424, 629, 1292, 1455
FaqI GGGAC 1 cut(s) 1411
FatI CATG 7 cut(s) 231, 255, 382, 420, 625, 1288, 1451
FauI CCCGC 1 cut(s) 206
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 1 cut(s) 1300
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 1 cut(s) 695
FriOI GRGCYC 3 cut(s) 240, 396, 1091
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 4 cut(s) 1070, 1167, 1176, 1458
FspI TGCGCA 1 cut(s) 997
GlaI GCGC 1 cut(s) 997
GluI GCNGC 1 cut(s) 290
HaeIII GGCC 1 cut(s) 260
HapII CCGG 4 cut(s) 261, 274, 293, 780
HgaI GACGC 2 cut(s) 286, 1090
HhaI GCGC 1 cut(s) 998
Hin1II CATG 7 cut(s) 235, 259, 386, 424, 629, 1292, 1455
Hin6I GCGC 1 cut(s) 996
HinP1I GCGC 1 cut(s) 996
HinfI GANTC 7 cut(s) 249, 695, 769, 788, 794, 872, 1030
HpaII CCGG 4 cut(s) 261, 274, 293, 780
HphI GGTGA 3 cut(s) 82, 296, 613
Hpy166II GTNNAC 2 cut(s) 514, 623
Hpy188I TCNGA 7 cut(s) 35, 354, 787, 793, 850, 1305, 1327
Hpy188III TCNNGA 8 cut(s) 241, 322, 721, 1027, 1127, 1167, 1198, 1289
Hpy8I GTNNAC 2 cut(s) 514, 623
Hpy99I CGWCG 3 cut(s) 448, 756, 1343
HpyAV CCTTC 6 cut(s) 37, 69, 189, 568, 1306, 1332
HpyCH4III ACNGT 3 cut(s) 655, 1191, 1366
HpyCH4IV ACGT 2 cut(s) 87, 1341
HpyCH4V TGCA 9 cut(s) 548, 645, 950, 1250, 1280, 1371, 1403, 1418, 1451
HpyF10VI GCNNNNNNNGC 5 cut(s) 219, 235, 286, 295, 559
HpyF3I CTNAG 2 cut(s) 517, 1004
HpySE526I ACGT 2 cut(s) 87, 1341
Hsp92II CATG 7 cut(s) 235, 259, 386, 424, 629, 1292, 1455
HspAI GCGC 1 cut(s) 996
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 1300
LmnI GCTCC 5 cut(s) 235, 355, 379, 838, 988
LweI GCATC 2 cut(s) 1063, 1237
MaeI CTAG 4 cut(s) 1070, 1167, 1176, 1458
MaeII ACGT 2 cut(s) 87, 1341
MaeIII GTNAC 2 cut(s) 88, 852
MboII GAAGA 9 cut(s) 106, 121, 447, 450, 650, 914, 922, 1115, 1266
MflI RGATCY 2 cut(s) 71, 1171
MhlI GDGCHC 3 cut(s) 240, 396, 1091
MluCI AATT 8 cut(s) 163, 282, 732, 917, 939, 1135, 1147, 1239
MlyI GAGTC 1 cut(s) 763
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 921
MseI TTAA 5 cut(s) 615, 633, 1058, 1185, 1308
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 4 cut(s) 261, 274, 293, 780
MspR9I CCNGG 1 cut(s) 780
MssI GTTTAAAC 1 cut(s) 1186
Mva1269I GAATGC 1 cut(s) 264
MwoI GCNNNNNNNGC 5 cut(s) 219, 235, 286, 295, 559
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 780
NcoI CCATGG 1 cut(s) 420
NdeI CATATG 1 cut(s) 739
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 7 cut(s) 235, 259, 386, 424, 629, 1292, 1455
NlaIV GGNNCC 5 cut(s) 191, 351, 778, 847, 984
NmuCI GTSAC 1 cut(s) 88
NsbI TGCGCA 1 cut(s) 997
NspI RCATGY 3 cut(s) 386, 629, 1455
PagI TCATGA 1 cut(s) 1288
PaqCI CACCTGC 1 cut(s) 361
PctI GAATGC 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 921
PfeI GAWTC 6 cut(s) 249, 695, 788, 794, 872, 1030
PflMI CCANNNNNTGG 3 cut(s) 232, 313, 657
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 763
PmeI GTTTAAAC 1 cut(s) 1186
PpsI GAGTC 1 cut(s) 763
Psp124BI GAGCTC 2 cut(s) 240, 396
PspN4I GGNNCC 5 cut(s) 191, 351, 778, 847, 984
PspPI GGNCC 1 cut(s) 776
PstI CTGCAG 2 cut(s) 647, 1405
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 2 cut(s) 71, 1171
RsaI GTAC 3 cut(s) 200, 591, 898
RsaNI GTAC 3 cut(s) 199, 590, 897
SacI GAGCTC 2 cut(s) 240, 396
SaqAI TTAA 5 cut(s) 615, 633, 1058, 1185, 1308
SatI GCNGC 1 cut(s) 290
Sau96I GGNCC 1 cut(s) 776
ScaI AGTACT 1 cut(s) 591
SchI GAGTC 1 cut(s) 763
ScrFI CCNGG 1 cut(s) 780
SduI GDGCHC 3 cut(s) 240, 396, 1091
SfaNI GCATC 2 cut(s) 1063, 1237
SfcI CTRYAG 2 cut(s) 643, 1401
SgrAI CRCCGGYG 1 cut(s) 292
SinI GGWCC 1 cut(s) 776
SmlI CTYRAG 2 cut(s) 239, 719
SmoI CTYRAG 2 cut(s) 239, 719
Sse9I AATT 8 cut(s) 163, 282, 732, 917, 939, 1135, 1147, 1239
SsiI CCGC 2 cut(s) 213, 290
SspI AATATT 1 cut(s) 1285
SspMI CTAG 4 cut(s) 1070, 1167, 1176, 1458
SstI GAGCTC 2 cut(s) 240, 396
StyD4I CCNGG 1 cut(s) 778
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 3 cut(s) 655, 1191, 1366
TaiI ACGT 2 cut(s) 90, 1344
TasI AATT 8 cut(s) 163, 282, 732, 917, 939, 1135, 1147, 1239
TatI WGTACW 1 cut(s) 589
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 6 cut(s) 249, 695, 788, 794, 872, 1030
Tru1I TTAA 5 cut(s) 615, 633, 1058, 1185, 1308
Tru9I TTAA 5 cut(s) 615, 633, 1058, 1185, 1308
TscAI CASTG 2 cut(s) 658, 835
TseFI GTSAC 1 cut(s) 88
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 7 cut(s) 17, 450, 651, 726, 726, 1091, 1277
TspGWI ACGGA 3 cut(s) 261, 857, 1436
TspRI CASTG 2 cut(s) 658, 835
Van91I CCANNNNNTGG 3 cut(s) 232, 313, 657
VpaK11BI GGWCC 1 cut(s) 776
XagI CCTNNNNNAGG 1 cut(s) 840
XapI RAATTY 2 cut(s) 732, 917
XbaI TCTAGA 1 cut(s) 1166
XceI RCATGY 3 cut(s) 386, 629, 1455
XmnI GAANNNNTTC 1 cut(s) 921
XspI CTAG 4 cut(s) 1070, 1167, 1176, 1458
ZrmI AGTACT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.