Prupe.5G065000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0015400
Physical Location & Seq
Forward (+)
7912225 .. 7914172
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G065000.1

Sequence Viewer

Length: 1485 bp
ATGAACCTCCTCACCAGAAACAGACCCACAGCGAGACCATGGCCCACCAGAAAACAACCCTCGTACAGGTCCCCACTCATCTACCTCACTCTCCTCTTTTCCCTTCTCTTCTTCTTCTCCTACTTCTTCTCTCTCACTCCCAATTCTCTCCTCTCCAACAAGACCTCAATTTCCGATTCCCCAAAATGCCCCTCCCTTGGAGGAGGAAAGTTCCTCTGGTACGCTCCCCACAGTGGCTTCAGCAACCAGCTTTCCGAGTTCAAGAACGCCGTTTTGATGGCCGCCATTTTGAACCGGACCTTGGTTGTCCCTCCGGTTCTCGATCACCATGCTGTTGCTCTCGGTAGTTGCCCCAAGTTTCGGGTTTTGAGTGCCAATGAGATCCGAATTTCGGTCTGGGATCATATCGTTGAGCTCATTCGGAGTGGGAGGTATGTTTCCATGGCTGACATTGTTGACGTATCATCATTAGTTTCTTCTTCTTTAGTTCGAGTCATAGATTTCAGGGTTTTTATATCCTTATGGTGCAATGTGAACGAAGATTTTGCTTGCTACAATGAGTTGGATAAGCATGCCTCTTTGCTTGAAAGACTTAAGCAATGTGGATCTCTACTATCTGGGCTCAATGGTGATGTAAAGTGTTTATATGCTGTTAATGAAGACTGCAGAACTACTGTTTGGACATACCAAAGTGGTAATTTGGATGGGGCATTGGATTCTTTCCAACCTGATGAACAACTCAAGAAGAAAAAGAAAATTTCATATGTTAGGAAACGCCGAGATGTATATAATACTCTCGGACCTGGTTCTGAAGCTGAATCAGCTACTGTACTGGCATTTGGGAGCCTTTTCACTCTTCCTTACAAACGTTCGGAGCTATACATTGATATTCATGATGCTCCAAGAGATCAAGGGATAAAAACTCTGATTGAGAAGATTGAGTTTCTTCCATTTGCCCCTGAAATTCTGAGCGCTGGGAAGAAGTTTGCTTATGGTACCATAAAGACTCCTTTTCTTTGTGCACAGCTCAGATTGCTAGATGGGCAGTTCAAGAACCATTGGAAGGCTACTTTTCTGAAGTTCAAACAAACAGTGGATGCTTTGATGCAAGGCCCTCTTCCTATTCATATTTTCGTGATGACCGATCTTCCTAAAAATAATTGGACAGGAAGTTACTTGGGGGAATTGGTTAGAGATTCACGTCAGTTTAAACTGTTTTTCCTGAAAGAAAGAGATGAGCTGATAATACAAACTGCTAAAAGAATCGTGGATGCAGGTCATGGCTTGAAGTTTGGGACTGTTCCAAAGAAACATGATGGCACTGGTCAGATTGAGAAGGATTGTCCTCCAGGACTACCTGATGTTCTTCTATACATAGAGCAAACTGTTTGCAGTTGTGCTTCCCTTGGATTTGTTGGGACAGCAGGGTCAACTATTGCAGACATTATAGAGTCGATGAGGAAGTTTGGTGCATGTTCTAGCTAG

Protein Analysis

495

Amino Acids

55.37

Weight (kDa)

9.03

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17430
fragaria_vesca FvH4_5g19490
malus_domestica MD04G1060600.v1.1
prunus_persica Prupe.5G065000_v2.0.a1
pyrus_communis pycom04g05440 pycom06g04540
rosa_chinensis RchiOBHm_Chr7g0204071
rosa_laevigata RLG00000003490
rosa_multiflora Rmu_co8337455.1_g000001
rosa_roxburghii Rroxscaffold_3G00253380
rosa_rugosa Rorug07G0080400
rosa_samantha Rh7AG210400 Rh7BG209000 Rh7CG222800 Rh7DG217100
rosa_wichuraiana Rw7G018320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1426
Acc36I ACCTGC 1 cut(s) 1265
Acc65I GGTACC 1 cut(s) 995
AccB1I GGYRCC 1 cut(s) 995
AciI CCGC 1 cut(s) 282
AclI AACGTT 1 cut(s) 868
AclWI GGATC 3 cut(s) 376, 408, 613
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 3 cut(s) 387, 756, 963
AcuI CTGAAG 3 cut(s) 223, 831, 1097
AfaI GTAC 4 cut(s) 65, 221, 831, 997
AfeI AGCGCT 1 cut(s) 973
AfiI CCNNNNNNNGG 7 cut(s) 66, 197, 233, 301, 360, 391, 1063
AflII CTTAAG 1 cut(s) 593
AgsI TTSAA 6 cut(s) 262, 292, 587, 1051, 1084, 1288
AjiI CACGTC 1 cut(s) 1202
AjnI CCWGG 2 cut(s) 802, 1348
AjuI GAANNNNNNNTTGG 4 cut(s) 284, 316, 661, 693
AluBI AGCT 8 cut(s) 250, 415, 815, 824, 877, 1027, 1240, 1482
AluI AGCT 8 cut(s) 250, 415, 815, 824, 877, 1027, 1240, 1482
Alw21I GWGCWC 2 cut(s) 417, 1024
Alw26I GTCTC 1 cut(s) 28
Alw44I GTGCAC 1 cut(s) 1020
AlwI GGATC 3 cut(s) 376, 408, 613
AlwNI CAGNNNCTG 1 cut(s) 827
Aor51HI AGCGCT 1 cut(s) 973
AoxI GGCC 3 cut(s) 41, 279, 1111
ApaLI GTGCAC 1 cut(s) 1020
ApoI RAATTY 3 cut(s) 387, 756, 963
Asp718I GGTACC 1 cut(s) 995
AspLEI GCGC 1 cut(s) 974
AspS9I GGNCC 5 cut(s) 42, 69, 297, 800, 1112
AsuHPI GGTGA 3 cut(s) 4, 317, 641
AvaII GGWCC 3 cut(s) 69, 297, 800
BaeGI GKGCMC 1 cut(s) 1024
BanI GGYRCC 1 cut(s) 995
BanII GRGCYC 2 cut(s) 417, 624
BbsI GAAGAC 1 cut(s) 666
Bbv12I GWGCWC 2 cut(s) 417, 1024
BccI CCATC 4 cut(s) 271, 698, 1034, 1310
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 4 cut(s) 311, 345, 527, 561
BciT130I CCWGG 2 cut(s) 804, 1350
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 3 cut(s) 1037, 1479, 1483
BfmI CTRYAG 1 cut(s) 664
BfoI RGCGCY 1 cut(s) 975
BfrI CTTAAG 1 cut(s) 593
BfuAI ACCTGC 1 cut(s) 1265
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 2 cut(s) 804, 1350
Bme18I GGWCC 3 cut(s) 69, 297, 800
BmgBI CACGTC 1 cut(s) 1202
BmgT120I GGNCC 5 cut(s) 42, 69, 297, 800, 1112
BmiI GGNNCC 3 cut(s) 71, 845, 997
BmrFI CCNGG 2 cut(s) 804, 1350
BmsI GCATC 4 cut(s) 886, 1087, 1095, 1261
BpiI GAAGAC 1 cut(s) 666
BpmI CTGGAG 1 cut(s) 1332
BpuEI CTTGAG 1 cut(s) 725
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 5 cut(s) 38, 196, 300, 441, 1405
BsaWI WCCGGW 2 cut(s) 294, 313
Bsc4I CCNNNNNNNGG 7 cut(s) 66, 197, 233, 301, 360, 391, 1063
Bse1I ACTGG 2 cut(s) 837, 1327
Bse3DI GCAATG 2 cut(s) 535, 605
BseBI CCWGG 2 cut(s) 804, 1350
BseDI CCNNGG 5 cut(s) 38, 196, 300, 441, 1405
BseGI GGATG 3 cut(s) 709, 1102, 1276
BseLI CCNNNNNNNGG 7 cut(s) 66, 197, 233, 301, 360, 391, 1063
BseMI GCAATG 2 cut(s) 535, 605
BseMII CTCAG 2 cut(s) 959, 1042
BseNI ACTGG 2 cut(s) 837, 1327
BseRI GAGGAG 3 cut(s) 83, 140, 216
BseSI GKGCMC 1 cut(s) 1024
BseYI CCCAGC 1 cut(s) 974
BshFI GGCC 3 cut(s) 43, 281, 1113
BshNI GGYRCC 1 cut(s) 995
BsiHKAI GWGCWC 2 cut(s) 417, 1024
BsiSI CCGG 2 cut(s) 295, 314
BslFI GGGAC 4 cut(s) 55, 293, 1309, 1432
BslI CCNNNNNNNGG 7 cut(s) 66, 197, 233, 301, 360, 391, 1063
BsmAI GTCTC 1 cut(s) 28
BsmFI GGGAC 4 cut(s) 55, 293, 1309, 1432
BsnI GGCC 3 cut(s) 43, 281, 1113
Bso31I GGTCTC 1 cut(s) 28
Bsp1286I GDGCHC 3 cut(s) 417, 624, 1024
Bsp143I GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
Bsp19I CCATGG 2 cut(s) 38, 441
BspACI CCGC 1 cut(s) 282
BspANI GGCC 3 cut(s) 43, 281, 1113
BspCNI CTCAG 2 cut(s) 960, 1041
BspHI TCATGA 1 cut(s) 892
BspLI GGNNCC 3 cut(s) 71, 845, 997
BspMAI CTGCAG 1 cut(s) 668
BspMI ACCTGC 1 cut(s) 1265
BspPI GGATC 3 cut(s) 376, 408, 613
BspT107I GGYRCC 1 cut(s) 995
BspTI CTTAAG 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 28
BsrDI GCAATG 2 cut(s) 535, 605
BsrI ACTGG 2 cut(s) 837, 1327
BssECI CCNNGG 5 cut(s) 38, 196, 300, 441, 1405
BssMI GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
BssT1I CCWWGG 5 cut(s) 38, 196, 300, 441, 1405
Bst2UI CCWGG 2 cut(s) 804, 1350
Bst4CI ACNGT 7 cut(s) 233, 676, 829, 1093, 1215, 1300, 1387
Bst6I CTCTTC 3 cut(s) 113, 861, 1122
BstAFI CTTAAG 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 550, 573
BstDEI CTNAG 2 cut(s) 968, 1028
BstDSI CCRYGG 2 cut(s) 38, 441
BstENI CCTNNNNNAGG 1 cut(s) 64
BstF5I GGATG 3 cut(s) 709, 1102, 1276
BstH2I RGCGCY 1 cut(s) 975
BstHHI GCGC 1 cut(s) 974
BstKTI GATC 6 cut(s) 325, 384, 403, 608, 910, 1147
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
BstMWI GCNNNNNNNGC 3 cut(s) 821, 1033, 1042
BstNI CCWGG 2 cut(s) 804, 1350
BstNSI RCATGY 2 cut(s) 575, 1476
BstSCI CCNGG 2 cut(s) 802, 1348
BstSFI CTRYAG 1 cut(s) 664
BstSLI GKGCMC 1 cut(s) 1024
BstV2I GAAGAC 1 cut(s) 666
BstX2I RGATCY 2 cut(s) 381, 605
BstYI RGATCY 2 cut(s) 381, 605
BsuRI GGCC 3 cut(s) 43, 281, 1113
BtgI CCRYGG 2 cut(s) 38, 441
BtrI CACGTC 1 cut(s) 1202
BtsCI GGATG 3 cut(s) 709, 1102, 1276
BtsIMutI CAGTG 3 cut(s) 238, 1098, 1320
BveI ACCTGC 1 cut(s) 1265
Cac8I GCNNGC 2 cut(s) 550, 573
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 892
CfoI GCGC 1 cut(s) 974
Cfr13I GGNCC 5 cut(s) 42, 69, 297, 800, 1112
CsiI ACCWGGT 1 cut(s) 802
Csp6I GTAC 4 cut(s) 64, 220, 830, 996
CviAII CATG 8 cut(s) 39, 329, 442, 572, 893, 1280, 1313, 1473
CviQI GTAC 4 cut(s) 64, 220, 830, 996
DdeI CTNAG 2 cut(s) 968, 1028
DpnI GATC 6 cut(s) 324, 383, 402, 607, 909, 1146
DpnII GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
DraI TTTAAA 1 cut(s) 1210
DrdI GACNNNNNNGTC 1 cut(s) 1426
DseDI GACNNNNNNGTC 1 cut(s) 1426
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 3 cut(s) 113, 861, 1122
EarI CTCTTC 3 cut(s) 113, 861, 1122
Ecl136II GAGCTC 1 cut(s) 415
Eco130I CCWWGG 5 cut(s) 38, 196, 300, 441, 1405
Eco24I GRGCYC 2 cut(s) 417, 624
Eco31I GGTCTC 1 cut(s) 28
Eco47I GGWCC 3 cut(s) 69, 297, 800
Eco47III AGCGCT 1 cut(s) 973
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 3 cut(s) 223, 831, 1097
EcoICRI GAGCTC 1 cut(s) 415
EcoNI CCTNNNNNAGG 1 cut(s) 64
EcoO109I RGGNCCY 2 cut(s) 69, 1112
EcoRII CCWGG 2 cut(s) 802, 1348
EcoT14I CCWWGG 5 cut(s) 38, 196, 300, 441, 1405
EcoT38I GRGCYC 2 cut(s) 417, 624
ErhI CCWWGG 5 cut(s) 38, 196, 300, 441, 1405
FaeI CATG 8 cut(s) 42, 332, 445, 575, 896, 1283, 1316, 1476
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FaqI GGGAC 4 cut(s) 55, 293, 1309, 1432
FatI CATG 8 cut(s) 38, 328, 441, 571, 892, 1279, 1312, 1472
FauNDI CATATG 1 cut(s) 763
Fnu4HI GCNGC 1 cut(s) 282
FokI GGATG 3 cut(s) 716, 1109, 1283
FriOI GRGCYC 2 cut(s) 417, 624
Fsp4HI GCNGC 1 cut(s) 282
FspBI CTAG 3 cut(s) 1037, 1479, 1483
GlaI GCGC 1 cut(s) 973
GluI GCNGC 1 cut(s) 282
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 1 cut(s) 1332
HaeII RGCGCY 1 cut(s) 975
HaeIII GGCC 3 cut(s) 43, 281, 1113
HapII CCGG 2 cut(s) 295, 314
HhaI GCGC 1 cut(s) 974
Hin1II CATG 8 cut(s) 42, 332, 445, 575, 896, 1283, 1316, 1476
Hin6I GCGC 1 cut(s) 972
HinP1I GCGC 1 cut(s) 972
HincII GTYRAC 2 cut(s) 457, 1431
HindII GTYRAC 2 cut(s) 457, 1431
HinfI GANTC 8 cut(s) 176, 492, 716, 818, 1006, 1196, 1263, 1451
HpaII CCGG 2 cut(s) 295, 314
HphI GGTGA 3 cut(s) 4, 317, 641
Hpy166II GTNNAC 4 cut(s) 457, 535, 1022, 1431
Hpy188III TCNNGA 7 cut(s) 262, 320, 742, 893, 1051, 1135, 1222
Hpy8I GTNNAC 4 cut(s) 457, 535, 1022, 1431
HpyAV CCTTC 3 cut(s) 113, 1057, 1330
HpyCH4III ACNGT 7 cut(s) 233, 676, 829, 1093, 1215, 1300, 1387
HpyCH4IV ACGT 3 cut(s) 459, 868, 1201
HpyCH4V TGCA 8 cut(s) 528, 666, 1022, 1108, 1274, 1392, 1439, 1472
HpyF10VI GCNNNNNNNGC 3 cut(s) 821, 1033, 1042
HpyF3I CTNAG 2 cut(s) 968, 1028
HpySE526I ACGT 3 cut(s) 459, 868, 1201
Hsp92II CATG 8 cut(s) 42, 332, 445, 575, 896, 1283, 1316, 1476
HspAI GCGC 1 cut(s) 972
KpnI GGTACC 1 cut(s) 999
Kzo9I GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
LmnI GCTCC 4 cut(s) 229, 843, 874, 904
LweI GCATC 4 cut(s) 886, 1087, 1095, 1261
MabI ACCWGGT 1 cut(s) 802
MaeI CTAG 3 cut(s) 1037, 1479, 1483
MaeII ACGT 3 cut(s) 459, 868, 1201
MaeIII GTNAC 1 cut(s) 1172
MalI GATC 6 cut(s) 324, 383, 402, 607, 909, 1146
MboI GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
MflI RGATCY 2 cut(s) 381, 605
MhlI GDGCHC 3 cut(s) 417, 624, 1024
MluCI AATT 8 cut(s) 142, 168, 387, 697, 756, 963, 1159, 1184
MlyI GAGTC 3 cut(s) 501, 1000, 1460
MmeI TCCRAC 3 cut(s) 180, 543, 748
MseI TTAA 3 cut(s) 594, 654, 1209
MspCI CTTAAG 1 cut(s) 593
MspI CCGG 2 cut(s) 295, 314
MspR9I CCNGG 2 cut(s) 804, 1350
MssI GTTTAAAC 1 cut(s) 1210
MvaI CCWGG 2 cut(s) 804, 1350
MwoI GCNNNNNNNGC 3 cut(s) 821, 1033, 1042
NcoI CCATGG 2 cut(s) 38, 441
NdeI CATATG 1 cut(s) 763
NdeII GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
NlaIII CATG 8 cut(s) 42, 332, 445, 575, 896, 1283, 1316, 1476
NlaIV GGNNCC 3 cut(s) 71, 845, 997
NmeAIII GCCGAG 1 cut(s) 803
NspI RCATGY 2 cut(s) 575, 1476
PaeI GCATGC 1 cut(s) 575
PagI TCATGA 1 cut(s) 892
PcsI WCGNNNNNNNCGW 1 cut(s) 1140
PfeI GAWTC 5 cut(s) 176, 716, 818, 1196, 1263
PfoI TCCNGGA 1 cut(s) 1348
PkrI GCNGC 1 cut(s) 283
PleI GAGTC 3 cut(s) 500, 1000, 1459
PmeI GTTTAAAC 1 cut(s) 1210
PpsI GAGTC 3 cut(s) 500, 1000, 1459
PpuMI RGGWCCY 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 417
Psp1406I AACGTT 1 cut(s) 868
Psp5II RGGWCCY 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 802, 1348
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 2 cut(s) 802, 1348
PspN4I GGNNCC 3 cut(s) 71, 845, 997
PspPI GGNCC 5 cut(s) 42, 69, 297, 800, 1112
PspPPI RGGWCCY 1 cut(s) 69
PstI CTGCAG 1 cut(s) 668
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 381, 605
RsaI GTAC 4 cut(s) 65, 221, 831, 997
RsaNI GTAC 4 cut(s) 64, 220, 830, 996
SacI GAGCTC 1 cut(s) 417
SaqAI TTAA 3 cut(s) 594, 654, 1209
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 6 cut(s) 322, 381, 400, 605, 907, 1144
Sau96I GGNCC 5 cut(s) 42, 69, 297, 800, 1112
SchI GAGTC 3 cut(s) 501, 1000, 1460
ScrFI CCNGG 2 cut(s) 804, 1350
SduI GDGCHC 3 cut(s) 417, 624, 1024
SexAI ACCWGGT 1 cut(s) 802
SfaNI GCATC 4 cut(s) 886, 1087, 1095, 1261
SfcI CTRYAG 1 cut(s) 664
SinI GGWCC 3 cut(s) 69, 297, 800
SmlI CTYRAG 2 cut(s) 593, 740
SmoI CTYRAG 2 cut(s) 593, 740
SphI GCATGC 1 cut(s) 575
Sse9I AATT 8 cut(s) 142, 168, 387, 697, 756, 963, 1159, 1184
SsiI CCGC 1 cut(s) 282
SspMI CTAG 3 cut(s) 1037, 1479, 1483
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 2 cut(s) 802, 1348
StyI CCWWGG 5 cut(s) 38, 196, 300, 441, 1405
TaaI ACNGT 7 cut(s) 233, 676, 829, 1093, 1215, 1300, 1387
TaiI ACGT 3 cut(s) 462, 871, 1204
TaqI TCGA 3 cut(s) 321, 490, 1454
TaqII GACCGA 2 cut(s) 382, 1157
TasI AATT 8 cut(s) 142, 168, 387, 697, 756, 963, 1159, 1184
TatI WGTACW 1 cut(s) 829
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 5 cut(s) 176, 716, 818, 1196, 1263
Tru1I TTAA 3 cut(s) 594, 654, 1209
Tru9I TTAA 3 cut(s) 594, 654, 1209
TscAI CASTG 3 cut(s) 238, 1098, 1327
TspDTI ATGAA 6 cut(s) 17, 672, 747, 750, 881, 1115
TspRI CASTG 3 cut(s) 238, 1098, 1327
Vha464I CTTAAG 1 cut(s) 593
VneI GTGCAC 1 cut(s) 1020
VpaK11BI GGWCC 3 cut(s) 69, 297, 800
XagI CCTNNNNNAGG 1 cut(s) 64
XapI RAATTY 3 cut(s) 387, 756, 963
XceI RCATGY 2 cut(s) 575, 1476
XspI CTAG 3 cut(s) 1037, 1479, 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.