Rw7G018320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015400
Physical Location & Seq
Reverse (-)
19661872 .. 19663849
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018320.1

Sequence Viewer

Length: 1428 bp
ATGAACCTCTTAACCTCAAAACCCACCACCCTCCGATCGAAAAGGCAACCCTCCCAAAGGTCCCCACTCCTCTACCTCACCCTCCTCTTCTCCCTCCTCATCTTCCTCTTCTACTTCTTCTCCCTCAGCCCCAATTCCCTCCTCACCGCCCAACTTTCCCATCCCCCAAAATGCCCTTCTCTGGGTGGAGACAAGTTCCTCTGGTACGCCCCCCACAGTGGCTTCAGCAACCAGCTCATGGAGTTGAAGAACGGAATCTTGATGGCCGGCATTTTGAACCGGACGCTGGTTGTGCCGCCGGTTCTCGATCACCACGCTGTGGCTCTCGGGAGTTGCCCCAAGTTTCGGGTTTCGGCTCCGAATGAGATCAGGGGTCAGGTCTGGGAGCATGTGATTGAGCTCATTGGGAGTGGGAGTTTCTTCTTCGTTAGTTCGAGTTTAGATTTCAGGGTTTTTATATCCTCATGGTGCAATGTGAACTTAGATTTTGCTTGCCATAATGACTTCAATGCACAGCCTTATTTGCTGGATAAATTAAAGGAATGTGGATCTGTACTAAGTGGTGTCAAAGGTAATGTTAAGTGTTTATATGCTGTTAATGAAGACTGCAGAACCACTGTTTGGACATACCAGAATGGTAATGAGGATGGGGCATTAGATTCTTTTCAACCTGATGAAAAACTCAAGAAAAAGAAGATTTCATATGTTAGGAAACGACGAGATGTATACAAGACTCTCGGACCGGGTTCTGAAGCTGAATCAGCTTCTGTGCTGGCATTTGGAAGCCTTTTCACTGCTCCTTACAAAGGTTCTGAGCTACTTATTGATATCCATGAATCTCCAAGAGATCAAAGGATAGGAAGTCTAATGGAGAAGATTGAGTTTCTTCCATTTGCTCCTGAAATTGTGAGTGCAGGAAAGAAGTTTGCTTTGGATACCATAAAGGCTCCTTTTCTATGTGCACAGCTTCGATTATTAGATGGGCAGTTCAAGAATCACCGGAAGGCTACTTTTCAAGGGCTAAAACAAAAGTTGGATGCTTTGATGCAGAGCCCTCTCCCCATTCATATCTTTGTGATGACTGATCTTCCTGAAAGTAATTGGACTGAAAATTACTTGGGGGATTTGGCTAGAGATTCAAATCAGTTTAAACTGTTTTTCCTGAAAGAAAGAGATGATTTGGTAATACAAACAGCTACAAGAATTGTTGATGCAGGCCATGGCTTGAAGTTTGGGTCTGTTGCAAATATTCATGATGGAACTGATCAGATTAAGAAGGATTGCCCTTCTGCAGTAATGCCCGATGTTCTTCTATACATAGAGCAAACTGTTTGCAGTTGTGCGTCTCTTGGATTTGTTGGGACTGCAGGATCAACTATTGCAGGGAGCATAGAGCTAATGAGAAAGTTTGGTGCATGTTCAAGCTAA

Protein Analysis

475

Amino Acids

52.74

Weight (kDa)

8.48

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17430
fragaria_vesca FvH4_5g19490
malus_domestica MD04G1060600.v1.1
prunus_persica Prupe.5G065000_v2.0.a1
pyrus_communis pycom04g05440 pycom06g04540
rosa_chinensis RchiOBHm_Chr7g0204071
rosa_laevigata RLG00000003490
rosa_multiflora Rmu_co8337455.1_g000001
rosa_roxburghii Rroxscaffold_3G00253380
rosa_rugosa Rorug07G0080400
rosa_samantha Rh7AG210400 Rh7BG209000 Rh7CG222800 Rh7DG217100
rosa_wichuraiana Rw7G018320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 238, 319, 621
AccI GTMKAC 1 cut(s) 726
AciI CCGC 2 cut(s) 147, 296
AclWI GGATC 2 cut(s) 556, 1378
AcoI YGGCCR 1 cut(s) 264
AcuI CTGAAG 2 cut(s) 208, 771
AdeI CACNNNGTG 1 cut(s) 319
AfaI GTAC 2 cut(s) 206, 555
AgsI TTSAA 9 cut(s) 247, 277, 508, 668, 991, 1016, 1140, 1228, 1422
AjuI GAANNNNNNNTTGG 6 cut(s) 160, 192, 604, 636, 914, 946
AluBI AGCT 9 cut(s) 235, 400, 755, 764, 817, 967, 1196, 1396, 1425
AluI AGCT 9 cut(s) 235, 400, 755, 764, 817, 967, 1196, 1396, 1425
Alw21I GWGCWC 2 cut(s) 402, 964
Alw26I GTCTC 2 cut(s) 183, 1350
Alw44I GTGCAC 1 cut(s) 960
AlwI GGATC 2 cut(s) 556, 1378
AlwNI CAGNNNCTG 1 cut(s) 767
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 264, 1216
ApaLI GTGCAC 1 cut(s) 960
AspS9I GGNCC 2 cut(s) 60, 740
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 4 cut(s) 70, 136, 302, 989
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 2 cut(s) 60, 740
BaeGI GKGCMC 1 cut(s) 964
BanII GRGCYC 2 cut(s) 402, 1055
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 2 cut(s) 402, 964
BbvCI CCTCAGC 1 cut(s) 125
BccI CCATC 5 cut(s) 168, 256, 641, 974, 1250
BciVI GTATCC 1 cut(s) 928
BclI TGATCA 1 cut(s) 1264
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 2 cut(s) 183, 1350
BfaI CTAG 1 cut(s) 1131
BfmI CTRYAG 3 cut(s) 607, 1290, 1365
BfuI GTATCC 1 cut(s) 928
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 2 cut(s) 60, 740
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 60, 740
BmiI GGNNCC 3 cut(s) 62, 357, 948
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 3 cut(s) 1027, 1035, 1201
BpiI GAAGAC 1 cut(s) 609
Bpu10I CCTNAGC 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 1140
BsaJI CCNNGG 1 cut(s) 1219
BsaWI WCCGGW 2 cut(s) 279, 999
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 400, 430
Bse118I RCCGGY 2 cut(s) 266, 298
Bse3DI GCAATG 1 cut(s) 478
Bse8I GATNNNNATC 1 cut(s) 1140
BseDI CCNNGG 1 cut(s) 1219
BseGI GGATG 3 cut(s) 160, 652, 1042
BseJI GATNNNNATC 1 cut(s) 1140
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 2 cut(s) 139, 804
BseRI GAGGAG 4 cut(s) 59, 74, 86, 131
BseSI GKGCMC 1 cut(s) 964
BsgI GTGCAG 1 cut(s) 933
Bsh1285I CGRYCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 266, 1218
BsiEI CGRYCG 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 402, 964
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 5 cut(s) 267, 280, 299, 743, 1000
BslFI GGGAC 2 cut(s) 46, 1375
BsmAI GTCTC 2 cut(s) 183, 1350
BsmBI CGTCTC 1 cut(s) 1350
BsmFI GGGAC 2 cut(s) 46, 1375
BsnI GGCC 2 cut(s) 266, 1218
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 3 cut(s) 402, 964, 1055
Bsp143I GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
Bsp19I CCATGG 1 cut(s) 1219
BspACI CCGC 2 cut(s) 147, 296
BspANI GGCC 2 cut(s) 266, 1218
BspCNI CTCAG 2 cut(s) 138, 805
BspHI TCATGA 1 cut(s) 1252
BspLI GGNNCC 3 cut(s) 62, 357, 948
BspMAI CTGCAG 3 cut(s) 611, 1294, 1369
BspPI GGATC 2 cut(s) 556, 1378
BsrDI GCAATG 1 cut(s) 478
BsrFI RCCGGY 2 cut(s) 266, 298
BssAI RCCGGY 2 cut(s) 266, 298
BssECI CCNNGG 1 cut(s) 1219
BssMI GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
BssNAI GTATAC 1 cut(s) 727
BssT1I CCWWGG 1 cut(s) 1219
Bst1107I GTATAC 1 cut(s) 727
Bst4CI ACNGT 4 cut(s) 218, 619, 1155, 1330
Bst6I CTCTTC 2 cut(s) 92, 113
BstC8I GCNNGC 4 cut(s) 268, 493, 774, 1216
BstDEI CTNAG 4 cut(s) 125, 481, 557, 813
BstDSI CCRYGG 1 cut(s) 1219
BstENI CCTNNNNNAGG 2 cut(s) 55, 804
BstF5I GGATG 3 cut(s) 160, 652, 1042
BstKTI GATC 8 cut(s) 38, 310, 369, 551, 850, 1087, 1267, 1373
BstMAI GTCTC 2 cut(s) 183, 1350
BstMBI GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
BstMCI CGRYCG 1 cut(s) 38
BstMWI GCNNNNNNNGC 3 cut(s) 292, 523, 761
BstNSI RCATGY 2 cut(s) 392, 1419
BstSCI CCNGG 1 cut(s) 742
BstSFI CTRYAG 3 cut(s) 607, 1290, 1365
BstSLI GKGCMC 1 cut(s) 964
BstV2I GAAGAC 1 cut(s) 609
BstX2I RGATCY 1 cut(s) 548
BstYI RGATCY 1 cut(s) 548
BstZ17I GTATAC 1 cut(s) 727
BsuI GTATCC 1 cut(s) 928
BsuRI GGCC 2 cut(s) 266, 1218
BtgI CCRYGG 1 cut(s) 1219
BtsCI GGATG 3 cut(s) 160, 652, 1042
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 3 cut(s) 223, 615, 792
Cac8I GCNNGC 4 cut(s) 268, 493, 774, 1216
CaiI CAGNNNCTG 1 cut(s) 767
CciI TCATGA 1 cut(s) 1252
Cfr10I RCCGGY 2 cut(s) 266, 298
Cfr13I GGNCC 2 cut(s) 60, 740
CpoI CGGWCCG 1 cut(s) 740
CseI GACGC 2 cut(s) 292, 1332
Csp6I GTAC 2 cut(s) 205, 554
CspI CGGWCCG 1 cut(s) 740
CviAII CATG 7 cut(s) 238, 389, 465, 833, 1220, 1253, 1416
CviQI GTAC 2 cut(s) 205, 554
DdeI CTNAG 4 cut(s) 125, 481, 557, 813
DpnI GATC 8 cut(s) 37, 309, 368, 550, 849, 1086, 1266, 1372
DpnII GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
DraI TTTAAA 1 cut(s) 1150
DraIII CACNNNGTG 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 2 cut(s) 92, 113
EarI CTCTTC 2 cut(s) 92, 113
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 1219
Eco24I GRGCYC 2 cut(s) 402, 1055
Eco32I GATATC 1 cut(s) 829
Eco47I GGWCC 2 cut(s) 60, 740
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 208, 771
Eco88I CYCGRG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 400
EcoNI CCTNNNNNAGG 2 cut(s) 55, 804
EcoO109I RGGNCCY 1 cut(s) 60
EcoRV GATATC 1 cut(s) 829
EcoT14I CCWWGG 1 cut(s) 1219
EcoT38I GRGCYC 2 cut(s) 402, 1055
ErhI CCWWGG 1 cut(s) 1219
Esp3I CGTCTC 1 cut(s) 1350
FaeI CATG 7 cut(s) 241, 392, 468, 836, 1223, 1256, 1419
FaqI GGGAC 2 cut(s) 46, 1375
FatI CATG 7 cut(s) 237, 388, 464, 832, 1219, 1252, 1415
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 1 cut(s) 1264
FblI GTMKAC 1 cut(s) 726
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 3 cut(s) 147, 659, 1049
FriOI GRGCYC 2 cut(s) 402, 1055
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 1 cut(s) 1131
GluI GCNGC 1 cut(s) 296
HaeIII GGCC 2 cut(s) 266, 1218
HapII CCGG 5 cut(s) 267, 280, 299, 743, 1000
HgaI GACGC 2 cut(s) 292, 1332
Hin1II CATG 7 cut(s) 241, 392, 468, 836, 1223, 1256, 1419
HinfI GANTC 7 cut(s) 255, 659, 733, 758, 836, 994, 1136
HpaII CCGG 5 cut(s) 267, 280, 299, 743, 1000
HphI GGTGA 4 cut(s) 70, 136, 302, 989
Hpy166II GTNNAC 3 cut(s) 478, 727, 962
Hpy188I TCNGA 6 cut(s) 35, 360, 740, 751, 814, 1269
Hpy188III TCNNGA 9 cut(s) 259, 305, 328, 685, 899, 991, 1091, 1162, 1253
Hpy8I GTNNAC 3 cut(s) 478, 727, 962
Hpy99I CGWCG 1 cut(s) 720
HpyAV CCTTC 4 cut(s) 186, 997, 1270, 1296
HpyCH4III ACNGT 4 cut(s) 218, 619, 1155, 1330
HpyF10VI GCNNNNNNNGC 3 cut(s) 292, 523, 761
HpyF3I CTNAG 4 cut(s) 125, 481, 557, 813
Hsp92II CATG 7 cut(s) 241, 392, 468, 836, 1223, 1256, 1419
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 1264
Kzo9I GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
LmnI GCTCC 6 cut(s) 361, 385, 802, 901, 952, 1386
LweI GCATC 3 cut(s) 1027, 1035, 1201
MaeI CTAG 1 cut(s) 1131
MalI GATC 8 cut(s) 37, 309, 368, 550, 849, 1086, 1266, 1372
MboI GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
MflI RGATCY 1 cut(s) 548
MhlI GDGCHC 3 cut(s) 402, 964, 1055
MluCI AATT 6 cut(s) 133, 533, 903, 1099, 1111, 1203
MlyI GAGTC 1 cut(s) 727
MmeI TCCRAC 1 cut(s) 1014
MroNI GCCGGC 1 cut(s) 266
MseI TTAA 6 cut(s) 11, 536, 579, 597, 1149, 1272
MspI CCGG 5 cut(s) 267, 280, 299, 743, 1000
MspR9I CCNGG 1 cut(s) 744
MssI GTTTAAAC 1 cut(s) 1150
MwoI GCNNNNNNNGC 3 cut(s) 292, 523, 761
NaeI GCCGGC 1 cut(s) 268
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 1219
NdeI CATATG 1 cut(s) 703
NdeII GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
NgoMIV GCCGGC 1 cut(s) 266
NlaIII CATG 7 cut(s) 241, 392, 468, 836, 1223, 1256, 1419
NlaIV GGNNCC 3 cut(s) 62, 357, 948
NspI RCATGY 2 cut(s) 392, 1419
PagI TCATGA 1 cut(s) 1252
PdiI GCCGGC 1 cut(s) 268
PfeI GAWTC 6 cut(s) 255, 659, 758, 836, 994, 1136
PflMI CCANNNNNTGG 3 cut(s) 238, 319, 621
PkrI GCNGC 1 cut(s) 297
Ple19I CGATCG 1 cut(s) 38
PleI GAGTC 1 cut(s) 727
PmeI GTTTAAAC 1 cut(s) 1150
PpsI GAGTC 1 cut(s) 727
PpuMI RGGWCCY 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 60
PspN4I GGNNCC 3 cut(s) 62, 357, 948
PspPI GGNCC 2 cut(s) 60, 740
PspPPI RGGWCCY 1 cut(s) 60
PstI CTGCAG 3 cut(s) 611, 1294, 1369
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 1 cut(s) 548
PvuI CGATCG 1 cut(s) 38
RsaI GTAC 2 cut(s) 206, 555
RsaNI GTAC 2 cut(s) 205, 554
Rsr2I CGGWCCG 1 cut(s) 740
RsrII CGGWCCG 1 cut(s) 740
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 6 cut(s) 11, 536, 579, 597, 1149, 1272
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 8 cut(s) 35, 307, 366, 548, 847, 1084, 1264, 1370
Sau96I GGNCC 2 cut(s) 60, 740
SchI GAGTC 1 cut(s) 727
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 3 cut(s) 402, 964, 1055
SfaNI GCATC 3 cut(s) 1027, 1035, 1201
SfcI CTRYAG 3 cut(s) 607, 1290, 1365
SinI GGWCC 2 cut(s) 60, 740
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 6 cut(s) 133, 533, 903, 1099, 1111, 1203
SsiI CCGC 2 cut(s) 147, 296
SspI AATATT 1 cut(s) 1249
SspMI CTAG 1 cut(s) 1131
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 1 cut(s) 1219
TaaI ACNGT 4 cut(s) 218, 619, 1155, 1330
TaqI TCGA 4 cut(s) 38, 306, 434, 970
TasI AATT 6 cut(s) 133, 533, 903, 1099, 1111, 1203
TatI WGTACW 1 cut(s) 553
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 6 cut(s) 255, 659, 758, 836, 994, 1136
Tru1I TTAA 6 cut(s) 11, 536, 579, 597, 1149, 1272
Tru9I TTAA 6 cut(s) 11, 536, 579, 597, 1149, 1272
TscAI CASTG 3 cut(s) 223, 622, 799
TspDTI ATGAA 7 cut(s) 17, 615, 690, 690, 849, 1055, 1241
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 3 cut(s) 223, 622, 799
Van91I CCANNNNNTGG 3 cut(s) 238, 319, 621
VneI GTGCAC 1 cut(s) 960
VpaK11BI GGWCC 2 cut(s) 60, 740
XagI CCTNNNNNAGG 2 cut(s) 55, 804
XceI RCATGY 2 cut(s) 392, 1419
XmiI GTMKAC 1 cut(s) 726
XspI CTAG 1 cut(s) 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.