pycom04g05440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015400
Physical Location & Seq
Forward (+)
5230522 .. 5232367
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05440.1

Sequence Viewer

Length: 1482 bp
ATGAATCTCTTCACCAGAAACAGACCCACAACGCGACCATGGCCTCTCAGAAGACACTCCTCCTCCGCCTCCCCTCTCCTCTACCTGACCCTCCTCTTCTCCCTCCTCCTCTTCCTCTCCTACTTCTTCTCCCTCACTCCCGATTCCCTCTTCATCAAGACCTCACTTTCCATTTCGCCAAATTGCCCCTCCTTCGGAGGACAAAAGTTCATCTGGTACGCTCCCCACAGTGGCTTCAGCAACCAGCTTACTGAGTTCAAGAACGCCCTTCTCATGGCCGCCATTCTGAACCGGACCTTGGTCGTCCCTCCAGTTCTCGATCACCACGCGGTTGCTCTCGGCAGTTGCCCAAAGTTTCGGGTTTTGAGCCCTGATGAGATCAGGATTTCGGTCTGGAATCATACGATTGAGCTCATTCGTAGTGGGAGGTATGTTTCCGTGGCTGACATTGTTGATATATCATCATTAGTTTCTTCTTCGTTAGTTCGAGTTGTAGATTTACGGGTTTTCTTATCCTCATGGTGCAATGTGAATGTAGATTTTACTTGCTACAATGAGTTGGATGCACGGGCCTCTGTGTTTGATAGACTACAGCAATGTAGGTCTCTTCTATCTGGGATGGATGGTGACGCAAAGTGTTCATATGCTGTTAACGAAGACTGCAGAACTACCGTTTGGACATACCAAAATGGTAATGAGGACGGGGCATTGGATTCTTTCCAGCCGGATGAACAACTAAAGAGGAAAAAGAAAATTTCATATGTTAGGAAGCAGCGAGATGTATATAACGCTCTTGGACCTGGTTCTGAAGCTGAATCGGCTATTCTGCTGGCATTTGGGAGTCTTTTCACTGCTCCTTACAAAGGTTCGCAGCTACACATTAGTATCCCTGAAGCTTCGAGTGATCGAAGGATACAAACGTTGATTGACAAGATTGAATTCCTTCGATTTGCCCCAGAAATCATAAGTGCAGGAAAGAAGTATGCTTATGATACTATAAAGGCTCCTTTTCTTTGTGCACAGCTCAGATTGCTAGACGGGCAGTTCAAGAACCACTGGAATGCTACTTTTTTGAAGCTAAAACAAACACTGGATGCTTTGACACGGAGCCCTCCCCCTATTCATATTTTTGTGATGACTGATCTTCCTGAAAGTAACTGGACAGGAACTTATTTGAGGGAATTGGTTGAAGATTTGCGTCAGTTTAAACTGTTCTTTCTGAAAGAAAAAGACGAGTTGATAATACAAACTGCTAAACAAATTGTGGATGCAAGTCATGGCTTGAAGCTTGGGACTGTCCCAAAGAATCATGATGGAACCGATCAGTTTAAGAAGGTTTGCCCTCCAGGATTGCCTGATGTTCTTCTATACATAGAGCAAACAATTTGTAGTTGTGCTTCTCTTGGATTTGTTGGGACTGCAGGGTCAACTATTGCAGACATTATAGAGTCGATGAGGAAGTTTGGTGTATGTTCTAGCTAA

Protein Analysis

494

Amino Acids

55.34

Weight (kDa)

8.61

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17430
fragaria_vesca FvH4_5g19490
malus_domestica MD04G1060600.v1.1
prunus_persica Prupe.5G065000_v2.0.a1
pyrus_communis pycom04g05440 pycom06g04540
rosa_chinensis RchiOBHm_Chr7g0204071
rosa_laevigata RLG00000003490
rosa_multiflora Rmu_co8337455.1_g000001
rosa_roxburghii Rroxscaffold_3G00253380
rosa_rugosa Rorug07G0080400
rosa_samantha Rh7AG210400 Rh7BG209000 Rh7CG222800 Rh7DG217100
rosa_wichuraiana Rw7G018320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1423
AccII CGCG 2 cut(s) 34, 329
AciI CCGC 3 cut(s) 66, 279, 329
AclI AACGTT 1 cut(s) 920
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 2 cut(s) 753, 938
AcuI CTGAAG 3 cut(s) 220, 828, 912
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 5 cut(s) 194, 230, 274, 298, 863
AgsI TTSAA 6 cut(s) 259, 938, 1048, 1075, 1190, 1285
AjnI CCWGG 2 cut(s) 799, 1345
AjuI GAANNNNNNNTTGG 4 cut(s) 281, 313, 658, 690
AluBI AGCT 9 cut(s) 247, 412, 812, 874, 896, 1024, 1078, 1288, 1479
AluI AGCT 9 cut(s) 247, 412, 812, 874, 896, 1024, 1078, 1288, 1479
Alw21I GWGCWC 2 cut(s) 414, 1021
Alw26I GTCTC 1 cut(s) 609
Alw44I GTGCAC 1 cut(s) 1017
AoxI GGCC 3 cut(s) 41, 276, 570
ApaLI GTGCAC 1 cut(s) 1017
ApeKI GCWGC 2 cut(s) 772, 871
ApoI RAATTY 2 cut(s) 753, 938
Asp700I GAANNNNTTC 2 cut(s) 8, 942
AspS9I GGNCC 3 cut(s) 294, 570, 797
AsuHPI GGTGA 3 cut(s) 4, 314, 638
AvaII GGWCC 2 cut(s) 294, 797
BaeGI GKGCMC 1 cut(s) 1021
BaeI ACNNNNGTAYC 4 cut(s) 868, 901, 905, 938
BanII GRGCYC 3 cut(s) 371, 414, 1112
BarI GAAGNNNNNNTAC 2 cut(s) 1381, 1413
BbsI GAAGAC 2 cut(s) 58, 663
Bbv12I GWGCWC 2 cut(s) 414, 1021
BbvI GCAGC 2 cut(s) 784, 883
BccI CCATC 3 cut(s) 613, 617, 1307
BciT130I CCWGG 2 cut(s) 801, 1347
BciVI GTATCC 2 cut(s) 896, 906
BcoDI GTCTC 1 cut(s) 609
BfaI CTAG 2 cut(s) 1034, 1476
BfmI CTRYAG 3 cut(s) 590, 661, 1419
BfuI GTATCC 2 cut(s) 896, 906
BisI GCNGC 3 cut(s) 279, 773, 872
BlsI GCNGC 3 cut(s) 280, 774, 873
Bme1390I CCNGG 2 cut(s) 801, 1347
Bme18I GGWCC 2 cut(s) 294, 797
BmgT120I GGNCC 3 cut(s) 294, 570, 797
BmiI GGNNCC 3 cut(s) 1005, 1109, 1318
BmrFI CCNGG 2 cut(s) 801, 1347
BmsI GCATC 3 cut(s) 553, 1084, 1258
BoxI GACNNNNGTC 1 cut(s) 299
BpiI GAAGAC 2 cut(s) 58, 663
BpmI CTGGAG 2 cut(s) 294, 1329
BsaI GGTCTC 1 cut(s) 609
BsaJI CCNNGG 3 cut(s) 38, 297, 438
BsaWI WCCGGW 1 cut(s) 291
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 113, 143
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 230, 274, 298, 863
Bse1I ACTGG 4 cut(s) 311, 1061, 1095, 1163
Bse3DI GCAATG 2 cut(s) 532, 602
BseBI CCWGG 2 cut(s) 801, 1347
BseDI CCNNGG 3 cut(s) 38, 297, 438
BseGI GGATG 6 cut(s) 568, 624, 628, 733, 1099, 1273
BseLI CCNNNNNNNGG 5 cut(s) 194, 230, 274, 298, 863
BseMI GCAATG 2 cut(s) 532, 602
BseMII CTCAG 3 cut(s) 61, 243, 1039
BseNI ACTGG 4 cut(s) 311, 1061, 1095, 1163
BseRI GAGGAG 6 cut(s) 49, 52, 68, 83, 95, 98
BseSI GKGCMC 1 cut(s) 1021
BseXI GCAGC 2 cut(s) 784, 883
BsgI GTGCAG 1 cut(s) 990
Bsh1236I CGCG 2 cut(s) 34, 329
BshFI GGCC 3 cut(s) 43, 278, 572
BsiHKAI GWGCWC 2 cut(s) 414, 1021
BsiSI CCGG 2 cut(s) 292, 725
BslFI GGGAC 4 cut(s) 290, 1283, 1306, 1429
BslI CCNNNNNNNGG 5 cut(s) 194, 230, 274, 298, 863
BsmAI GTCTC 1 cut(s) 609
BsmFI GGGAC 4 cut(s) 290, 1283, 1306, 1429
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 3 cut(s) 43, 278, 572
Bso31I GGTCTC 1 cut(s) 609
Bsp1286I GDGCHC 4 cut(s) 371, 414, 1021, 1112
Bsp143I GATC 5 cut(s) 319, 378, 904, 1141, 1321
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 3 cut(s) 66, 279, 329
BspANI GGCC 3 cut(s) 43, 278, 572
BspCNI CTCAG 3 cut(s) 60, 244, 1038
BspFNI CGCG 2 cut(s) 34, 329
BspHI TCATGA 1 cut(s) 1309
BspLI GGNNCC 3 cut(s) 1005, 1109, 1318
BspMAI CTGCAG 2 cut(s) 665, 1423
BspTNI GGTCTC 1 cut(s) 609
BsrDI GCAATG 2 cut(s) 532, 602
BsrI ACTGG 4 cut(s) 311, 1061, 1095, 1163
BssECI CCNNGG 3 cut(s) 38, 297, 438
BssMI GATC 5 cut(s) 319, 378, 904, 1141, 1321
BssT1I CCWWGG 2 cut(s) 38, 297
Bst2UI CCWGG 2 cut(s) 801, 1347
Bst4CI ACNGT 4 cut(s) 230, 673, 1212, 1297
Bst6I CTCTTC 5 cut(s) 14, 101, 116, 155, 612
BstC8I GCNNGC 1 cut(s) 831
BstDEI CTNAG 3 cut(s) 47, 252, 1025
BstDSI CCRYGG 2 cut(s) 38, 438
BstENI CCTNNNNNAGG 1 cut(s) 861
BstF5I GGATG 6 cut(s) 568, 624, 628, 733, 1099, 1273
BstFNI CGCG 2 cut(s) 34, 329
BstKTI GATC 5 cut(s) 322, 381, 907, 1144, 1324
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 5 cut(s) 319, 378, 904, 1141, 1321
BstMWI GCNNNNNNNGC 4 cut(s) 40, 818, 1030, 1039
BstNI CCWGG 2 cut(s) 801, 1347
BstPAI GACNNNNGTC 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 799, 1345
BstSFI CTRYAG 3 cut(s) 590, 661, 1419
BstSLI GKGCMC 1 cut(s) 1021
BstUI CGCG 2 cut(s) 34, 329
BstV1I GCAGC 2 cut(s) 784, 883
BstV2I GAAGAC 2 cut(s) 58, 663
BsuI GTATCC 2 cut(s) 896, 906
BsuRI GGCC 3 cut(s) 43, 278, 572
BtgI CCRYGG 2 cut(s) 38, 438
BtsCI GGATG 6 cut(s) 568, 624, 628, 733, 1099, 1273
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 4 cut(s) 235, 849, 1054, 1088
Cac8I GCNNGC 1 cut(s) 831
CciI TCATGA 1 cut(s) 1309
Cfr13I GGNCC 3 cut(s) 294, 570, 797
CseI GACGC 2 cut(s) 638, 1187
CsiI ACCWGGT 1 cut(s) 799
Csp6I GTAC 1 cut(s) 217
CviAII CATG 5 cut(s) 39, 274, 519, 1277, 1310
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 3 cut(s) 47, 252, 1025
DpnI GATC 5 cut(s) 321, 380, 906, 1143, 1323
DpnII GATC 5 cut(s) 319, 378, 904, 1141, 1321
DraI TTTAAA 1 cut(s) 1207
DrdI GACNNNNNNGTC 1 cut(s) 1423
DseDI GACNNNNNNGTC 1 cut(s) 1423
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 5 cut(s) 14, 101, 116, 155, 612
EarI CTCTTC 5 cut(s) 14, 101, 116, 155, 612
EciI GGCGGA 1 cut(s) 55
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 2 cut(s) 38, 297
Eco24I GRGCYC 3 cut(s) 371, 414, 1112
Eco31I GGTCTC 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 294, 797
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 3 cut(s) 220, 828, 912
EcoICRI GAGCTC 1 cut(s) 412
EcoNI CCTNNNNNAGG 1 cut(s) 861
EcoRI GAATTC 1 cut(s) 938
EcoRII CCWGG 2 cut(s) 799, 1345
EcoT14I CCWWGG 2 cut(s) 38, 297
EcoT38I GRGCYC 3 cut(s) 371, 414, 1112
ErhI CCWWGG 2 cut(s) 38, 297
FaeI CATG 5 cut(s) 42, 277, 522, 1280, 1313
FaqI GGGAC 4 cut(s) 290, 1283, 1306, 1429
FatI CATG 5 cut(s) 38, 273, 518, 1276, 1309
FauNDI CATATG 2 cut(s) 643, 760
Fnu4HI GCNGC 3 cut(s) 279, 773, 872
FokI GGATG 6 cut(s) 575, 631, 635, 740, 1106, 1280
FriOI GRGCYC 3 cut(s) 371, 414, 1112
Fsp4HI GCNGC 3 cut(s) 279, 773, 872
FspBI CTAG 2 cut(s) 1034, 1476
GluI GCNGC 3 cut(s) 279, 773, 872
GsuI CTGGAG 2 cut(s) 294, 1329
HaeIII GGCC 3 cut(s) 43, 278, 572
HapII CCGG 2 cut(s) 292, 725
HgaI GACGC 2 cut(s) 638, 1187
Hin1II CATG 5 cut(s) 42, 277, 522, 1280, 1313
HincII GTYRAC 2 cut(s) 652, 1428
HindII GTYRAC 2 cut(s) 652, 1428
HindIII AAGCTT 2 cut(s) 894, 1286
HinfI GANTC 8 cut(s) 4, 143, 397, 713, 815, 841, 1306, 1448
HpaI GTTAAC 1 cut(s) 652
HpaII CCGG 2 cut(s) 292, 725
HphI GGTGA 3 cut(s) 4, 314, 638
Hpy166II GTNNAC 3 cut(s) 652, 1019, 1428
Hpy188I TCNGA 6 cut(s) 50, 197, 288, 808, 1028, 1221
Hpy188III TCNNGA 9 cut(s) 140, 157, 259, 317, 382, 394, 1048, 1148, 1310
Hpy8I GTNNAC 3 cut(s) 652, 1019, 1428
HpyAV CCTTC 5 cut(s) 202, 278, 903, 953, 1327
HpyCH4III ACNGT 4 cut(s) 230, 673, 1212, 1297
HpyCH4IV ACGT 1 cut(s) 920
HpyCH4V TGCA 8 cut(s) 525, 566, 663, 971, 1019, 1271, 1421, 1436
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 818, 1030, 1039
HpyF3I CTNAG 3 cut(s) 47, 252, 1025
HpySE526I ACGT 1 cut(s) 920
Hsp92II CATG 5 cut(s) 42, 277, 522, 1280, 1313
KspAI GTTAAC 1 cut(s) 652
Kzo9I GATC 5 cut(s) 319, 378, 904, 1141, 1321
LmnI GCTCC 4 cut(s) 226, 859, 1009, 1107
Lsp1109I GCAGC 2 cut(s) 784, 883
LweI GCATC 3 cut(s) 553, 1084, 1258
MabI ACCWGGT 1 cut(s) 799
MaeI CTAG 2 cut(s) 1034, 1476
MaeII ACGT 1 cut(s) 920
MaeIII GTNAC 2 cut(s) 626, 1154
MalI GATC 5 cut(s) 321, 380, 906, 1143, 1323
MboI GATC 5 cut(s) 319, 378, 904, 1141, 1321
MhlI GDGCHC 4 cut(s) 371, 414, 1021, 1112
MluCI AATT 6 cut(s) 181, 753, 938, 1181, 1260, 1383
MlyI GAGTC 2 cut(s) 850, 1457
MmeI TCCRAC 1 cut(s) 540
MroXI GAANNNNTTC 2 cut(s) 8, 942
MseI TTAA 3 cut(s) 651, 1206, 1329
MslI CAYNNNNRTG 1 cut(s) 1059
MspI CCGG 2 cut(s) 292, 725
MspR9I CCNGG 2 cut(s) 801, 1347
MssI GTTTAAAC 1 cut(s) 1207
Mva1269I GAATGC 1 cut(s) 1066
MvaI CCWGG 2 cut(s) 801, 1347
MvnI CGCG 2 cut(s) 34, 329
MwoI GCNNNNNNNGC 4 cut(s) 40, 818, 1030, 1039
NcoI CCATGG 1 cut(s) 38
NdeI CATATG 2 cut(s) 643, 760
NdeII GATC 5 cut(s) 319, 378, 904, 1141, 1321
NlaIII CATG 5 cut(s) 42, 277, 522, 1280, 1313
NlaIV GGNNCC 3 cut(s) 1005, 1109, 1318
NmeAIII GCCGAG 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 626
PagI TCATGA 1 cut(s) 1309
PctI GAATGC 1 cut(s) 1066
PdmI GAANNNNTTC 2 cut(s) 8, 942
PfeI GAWTC 6 cut(s) 4, 143, 397, 713, 815, 1306
PfoI TCCNGGA 1 cut(s) 1345
PkrI GCNGC 3 cut(s) 280, 774, 873
PleI GAGTC 2 cut(s) 849, 1456
PmeI GTTTAAAC 1 cut(s) 1207
PpsI GAGTC 2 cut(s) 849, 1456
PshAI GACNNNNGTC 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 414
Psp1406I AACGTT 1 cut(s) 920
Psp6I CCWGG 2 cut(s) 799, 1345
PspGI CCWGG 2 cut(s) 799, 1345
PspN4I GGNNCC 3 cut(s) 1005, 1109, 1318
PspPI GGNCC 3 cut(s) 294, 570, 797
PstI CTGCAG 2 cut(s) 665, 1423
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 1059
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 3 cut(s) 651, 1206, 1329
SatI GCNGC 3 cut(s) 279, 773, 872
Sau3AI GATC 5 cut(s) 319, 378, 904, 1141, 1321
Sau96I GGNCC 3 cut(s) 294, 570, 797
SchI GAGTC 2 cut(s) 850, 1457
ScrFI CCNGG 2 cut(s) 801, 1347
SduI GDGCHC 4 cut(s) 371, 414, 1021, 1112
SexAI ACCWGGT 1 cut(s) 799
SfaNI GCATC 3 cut(s) 553, 1084, 1258
SfcI CTRYAG 3 cut(s) 590, 661, 1419
SinI GGWCC 2 cut(s) 294, 797
SmiMI CAYNNNNRTG 1 cut(s) 1059
Sse9I AATT 6 cut(s) 181, 753, 938, 1181, 1260, 1383
SsiI CCGC 3 cut(s) 66, 279, 329
SspMI CTAG 2 cut(s) 1034, 1476
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 2 cut(s) 799, 1345
StyI CCWWGG 2 cut(s) 38, 297
TaaI ACNGT 4 cut(s) 230, 673, 1212, 1297
TaiI ACGT 1 cut(s) 923
TaqI TCGA 6 cut(s) 318, 487, 899, 907, 946, 1451
TaqII GACCGA 1 cut(s) 379
TasI AATT 6 cut(s) 181, 753, 938, 1181, 1260, 1383
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 6 cut(s) 4, 143, 397, 713, 815, 1306
Tru1I TTAA 3 cut(s) 651, 1206, 1329
Tru9I TTAA 3 cut(s) 651, 1206, 1329
TscAI CASTG 4 cut(s) 235, 856, 1061, 1095
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 2 cut(s) 772, 871
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 7 cut(s) 17, 142, 199, 630, 744, 747, 1112
TspGWI ACGGA 2 cut(s) 427, 1120
TspRI CASTG 4 cut(s) 235, 856, 1061, 1095
VneI GTGCAC 1 cut(s) 1017
VpaK11BI GGWCC 2 cut(s) 294, 797
XagI CCTNNNNNAGG 1 cut(s) 861
XapI RAATTY 2 cut(s) 753, 938
XmnI GAANNNNTTC 2 cut(s) 8, 942
XspI CTAG 2 cut(s) 1034, 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.