RLG00000003490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015400
Physical Location & Seq
Forward (+)
49126234 .. 49128217
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003490

Sequence Viewer

Length: 1479 bp
ATGAACCTCTTAACCTCAAAACCTACCACCCTCCGATCATGGCACCCCAAAAGGCAACCCTCCCAAAGGTCCCCACTCCTCTACCTCACCCTCCTCTTCTCCCTCCTCATCTTCCTCTTCTACTTCTTCTCCCTCAGCCCCAATTCCCTCCTCACCGCCCAACTTTCCCATTCCCCAAAATGCCCTTCTCTGGGTGGAGACAAGTTCCTCTGGTACGCCCCCCACAGTGGCTTCAGCAACCAGCTCATGGAGTTAAAAAACGGAATCTTGATGGCCGGCATTTTGAACCGGACGCTGGTTGTGCCGCCGGTTCTCGATCACCACGCTGTGGCTCTCGGGAGTTGCCCCAAGTTTCGGGTTTCGGCTCCGAATGAGATCAGGGGTGAGGTCTGGGAGCATGTGATTGAGCTCATTAGGAGTGGGAGGTATGTATCCATGGCTGACATTGTTGATATATCATCATTAGTTTCTTCTTCGTTAGTTCGAGTTATAGAGTTCAGGGTTTTTATATCCTCATGGTGCAATGTGAACTTAGATTTTGCTTGCCATAATGAGTTCAGTGCACAGCCTTATTTGCTGGATAAACTAAAGGAATGTGGATCTGTACTAAGTGGTGTCAAAGGTAATGTTAAGTGTTTATATGCTGTTAATGAAGACTGCAGAACCACTGTTTGGACATACCAGAATGGTAATGAGGATGGGGCATTAGATTCTTTTCAACCTGATGACAAACTCAAGAAAAAGAAGATTTCATATGTTAGGAAACGACGAGATGTATACAAGACTCTTGGACCGGGTTCTGAAGCTGAATCAGCTTCTGTGCTGGCATTTGGAAGCCTTTTCACTGCTCCTTACAAAGGTTCTGAACTACTTATTGATATCCATGAATCTCCAAGAGATCAAAGGATAGGAAGTCTAATGGAGAAGATTGAGTTTCTTCCATTTGCTCCTGAAATTGTGAGTGCAGGAAAGAAGTTTGCTTTGGACACCATAAAGGCTCCTTTTCTATGTGCACAGCTTCGATTATTAGATGGGCAGTTCAAGAATCACCGGAAGGCTACTTTTCAAGGGCTAAAACAAAAGTTGGATGCTTTGATGCAGAGCCCTCTCCCCATTCATATCTTTGTGATGACTGATCTTCCTGAAAGTAATTGGACCAGAAATTACTTGGGGGATTTGGCTAGAGATTCAAATCAGTTTAAACTGTTTTTCCTGAAAGAAAGTGATGATTTGGTAATACAAACAGCTAAAAGAATTGTTGATGCAGGCCATGGCTTGAAGTTTGGGTCTGTCGCAAATATTCATGATGGAACTGATCAGATTAAGAAGGATTGTCCTTCTGCAGTAATACCCGATGTTCTTCTATACATAGAGCAAACTGTTTGCAGTTGTGCTTCTCTTGGATTTGTTGGGACTGCAGGATCAACTATTGCAGGGAGCATAGAGCTAATGAGAAAGTTTGGTGCATGTTCAAGCTAA

Protein Analysis

493

Amino Acids

54.7

Weight (kDa)

8.69

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015400)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17430
fragaria_vesca FvH4_5g19490
malus_domestica MD04G1060600.v1.1
prunus_persica Prupe.5G065000_v2.0.a1
pyrus_communis pycom04g05440 pycom06g04540
rosa_chinensis RchiOBHm_Chr7g0204071
rosa_laevigata RLG00000003490
rosa_multiflora Rmu_co8337455.1_g000001
rosa_roxburghii Rroxscaffold_3G00253380
rosa_rugosa Rorug07G0080400
rosa_samantha Rh7AG210400 Rh7BG209000 Rh7CG222800 Rh7DG217100
rosa_wichuraiana Rw7G018320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 42
AccB7I CCANNNNNTGG 3 cut(s) 247, 328, 672
AccI GTMKAC 1 cut(s) 777
AciI CCGC 2 cut(s) 156, 305
AclWI GGATC 2 cut(s) 607, 1429
AcoI YGGCCR 1 cut(s) 273
AcuI CTGAAG 2 cut(s) 217, 822
AdeI CACNNNGTG 1 cut(s) 328
AfaI GTAC 2 cut(s) 215, 606
AgsI TTSAA 7 cut(s) 286, 719, 1042, 1067, 1191, 1279, 1473
AjuI GAANNNNNNNTTGG 6 cut(s) 169, 201, 655, 687, 965, 997
AluBI AGCT 8 cut(s) 244, 409, 806, 815, 1018, 1247, 1447, 1476
AluI AGCT 8 cut(s) 244, 409, 806, 815, 1018, 1247, 1447, 1476
Alw21I GWGCWC 3 cut(s) 411, 565, 1015
Alw26I GTCTC 1 cut(s) 192
Alw44I GTGCAC 2 cut(s) 561, 1011
AlwI GGATC 2 cut(s) 607, 1429
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 2 cut(s) 273, 1267
ApaLI GTGCAC 2 cut(s) 561, 1011
AspS9I GGNCC 3 cut(s) 69, 791, 1155
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 5 cut(s) 79, 145, 311, 395, 1040
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 3 cut(s) 69, 791, 1155
BaeGI GKGCMC 2 cut(s) 565, 1015
BanI GGYRCC 1 cut(s) 42
BanII GRGCYC 2 cut(s) 411, 1106
BbsI GAAGAC 1 cut(s) 660
Bbv12I GWGCWC 3 cut(s) 411, 565, 1015
BbvCI CCTCAGC 1 cut(s) 134
BccI CCATC 4 cut(s) 265, 692, 1025, 1301
BciVI GTATCC 1 cut(s) 442
BclI TGATCA 1 cut(s) 1315
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 1 cut(s) 192
BfaI CTAG 1 cut(s) 1182
BfmI CTRYAG 3 cut(s) 658, 1341, 1416
BfuI GTATCC 1 cut(s) 442
BisI GCNGC 1 cut(s) 305
BlsI GCNGC 1 cut(s) 306
Bme1390I CCNGG 1 cut(s) 795
Bme18I GGWCC 3 cut(s) 69, 791, 1155
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 3 cut(s) 69, 791, 1155
BmiI GGNNCC 4 cut(s) 44, 71, 366, 999
BmrFI CCNGG 1 cut(s) 795
BmsI GCATC 3 cut(s) 1078, 1086, 1252
BpiI GAAGAC 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 719
BpuMI CCSGG 1 cut(s) 795
BsaBI GATNNNNATC 1 cut(s) 1191
BsaJI CCNNGG 2 cut(s) 435, 1270
BsaWI WCCGGW 2 cut(s) 288, 1050
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bse118I RCCGGY 2 cut(s) 275, 307
Bse3DI GCAATG 1 cut(s) 529
Bse8I GATNNNNATC 1 cut(s) 1191
BseDI CCNNGG 2 cut(s) 435, 1270
BseGI GGATG 2 cut(s) 703, 1093
BseJI GATNNNNATC 1 cut(s) 1191
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 1 cut(s) 148
BseRI GAGGAG 4 cut(s) 68, 83, 95, 140
BseSI GKGCMC 2 cut(s) 565, 1015
BsgI GTGCAG 1 cut(s) 984
BshFI GGCC 2 cut(s) 275, 1269
BshNI GGYRCC 1 cut(s) 42
BsiHKAI GWGCWC 3 cut(s) 411, 565, 1015
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 5 cut(s) 276, 289, 308, 794, 1051
BslFI GGGAC 2 cut(s) 55, 1426
BsmAI GTCTC 1 cut(s) 192
BsmFI GGGAC 2 cut(s) 55, 1426
BsnI GGCC 2 cut(s) 275, 1269
BsoBI CYCGRG 1 cut(s) 335
Bsp1286I GDGCHC 4 cut(s) 411, 565, 1015, 1106
Bsp143I GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
Bsp19I CCATGG 2 cut(s) 435, 1270
BspACI CCGC 2 cut(s) 156, 305
BspANI GGCC 2 cut(s) 275, 1269
BspCNI CTCAG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 4 cut(s) 44, 71, 366, 999
BspMAI CTGCAG 3 cut(s) 662, 1345, 1420
BspPI GGATC 2 cut(s) 607, 1429
BspT107I GGYRCC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 529
BsrFI RCCGGY 2 cut(s) 275, 307
BssAI RCCGGY 2 cut(s) 275, 307
BssECI CCNNGG 2 cut(s) 435, 1270
BssMI GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
BssNAI GTATAC 1 cut(s) 778
BssT1I CCWWGG 2 cut(s) 435, 1270
Bst1107I GTATAC 1 cut(s) 778
Bst4CI ACNGT 4 cut(s) 227, 670, 1206, 1381
Bst6I CTCTTC 2 cut(s) 101, 122
BstC8I GCNNGC 4 cut(s) 277, 544, 825, 1267
BstDEI CTNAG 3 cut(s) 134, 532, 608
BstDSI CCRYGG 2 cut(s) 435, 1270
BstENI CCTNNNNNAGG 2 cut(s) 64, 855
BstF5I GGATG 2 cut(s) 703, 1093
BstKTI GATC 8 cut(s) 38, 319, 378, 602, 901, 1138, 1318, 1424
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
BstMWI GCNNNNNNNGC 3 cut(s) 301, 574, 812
BstNSI RCATGY 2 cut(s) 401, 1470
BstSCI CCNGG 1 cut(s) 793
BstSFI CTRYAG 3 cut(s) 658, 1341, 1416
BstSLI GKGCMC 2 cut(s) 565, 1015
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 1 cut(s) 599
BstYI RGATCY 1 cut(s) 599
BstZ17I GTATAC 1 cut(s) 778
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 2 cut(s) 275, 1269
BtgI CCRYGG 2 cut(s) 435, 1270
BtsCI GGATG 2 cut(s) 703, 1093
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 4 cut(s) 232, 565, 666, 843
Cac8I GCNNGC 4 cut(s) 277, 544, 825, 1267
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 1 cut(s) 1303
Cfr10I RCCGGY 2 cut(s) 275, 307
Cfr13I GGNCC 3 cut(s) 69, 791, 1155
CseI GACGC 1 cut(s) 301
Csp6I GTAC 2 cut(s) 214, 605
CviAII CATG 9 cut(s) 39, 247, 398, 436, 516, 884, 1271, 1304, 1467
CviQI GTAC 2 cut(s) 214, 605
DdeI CTNAG 3 cut(s) 134, 532, 608
DpnI GATC 8 cut(s) 37, 318, 377, 601, 900, 1137, 1317, 1423
DpnII GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
DraI TTTAAA 1 cut(s) 1201
DraIII CACNNNGTG 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 101, 122
EarI CTCTTC 2 cut(s) 101, 122
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 2 cut(s) 435, 1270
Eco24I GRGCYC 2 cut(s) 411, 1106
Eco32I GATATC 1 cut(s) 880
Eco47I GGWCC 3 cut(s) 69, 791, 1155
Eco53kI GAGCTC 1 cut(s) 409
Eco57I CTGAAG 2 cut(s) 217, 822
Eco88I CYCGRG 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 409
EcoNI CCTNNNNNAGG 2 cut(s) 64, 855
EcoO109I RGGNCCY 1 cut(s) 69
EcoRV GATATC 1 cut(s) 880
EcoT14I CCWWGG 2 cut(s) 435, 1270
EcoT38I GRGCYC 2 cut(s) 411, 1106
ErhI CCWWGG 2 cut(s) 435, 1270
FaeI CATG 9 cut(s) 42, 250, 401, 439, 519, 887, 1274, 1307, 1470
FaqI GGGAC 2 cut(s) 55, 1426
FatI CATG 9 cut(s) 38, 246, 397, 435, 515, 883, 1270, 1303, 1466
FauNDI CATATG 1 cut(s) 754
FbaI TGATCA 1 cut(s) 1315
FblI GTMKAC 1 cut(s) 777
Fnu4HI GCNGC 1 cut(s) 305
FokI GGATG 2 cut(s) 710, 1100
FriOI GRGCYC 2 cut(s) 411, 1106
Fsp4HI GCNGC 1 cut(s) 305
FspBI CTAG 1 cut(s) 1182
GluI GCNGC 1 cut(s) 305
HaeIII GGCC 2 cut(s) 275, 1269
HapII CCGG 5 cut(s) 276, 289, 308, 794, 1051
HgaI GACGC 1 cut(s) 301
Hin1II CATG 9 cut(s) 42, 250, 401, 439, 519, 887, 1274, 1307, 1470
HinfI GANTC 7 cut(s) 264, 710, 784, 809, 887, 1045, 1187
HpaII CCGG 5 cut(s) 276, 289, 308, 794, 1051
HphI GGTGA 5 cut(s) 79, 145, 311, 395, 1040
Hpy166II GTNNAC 4 cut(s) 529, 563, 778, 1013
Hpy188I TCNGA 5 cut(s) 35, 369, 802, 865, 1320
Hpy188III TCNNGA 9 cut(s) 268, 314, 337, 736, 950, 1042, 1142, 1213, 1304
Hpy8I GTNNAC 4 cut(s) 529, 563, 778, 1013
Hpy99I CGWCG 1 cut(s) 771
HpyAV CCTTC 4 cut(s) 195, 1048, 1321, 1347
HpyCH4III ACNGT 4 cut(s) 227, 670, 1206, 1381
HpyF10VI GCNNNNNNNGC 3 cut(s) 301, 574, 812
HpyF3I CTNAG 3 cut(s) 134, 532, 608
Hsp92II CATG 9 cut(s) 42, 250, 401, 439, 519, 887, 1274, 1307, 1470
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
Ksp22I TGATCA 1 cut(s) 1315
Kzo9I GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
LmnI GCTCC 6 cut(s) 370, 394, 853, 952, 1003, 1437
LweI GCATC 3 cut(s) 1078, 1086, 1252
MaeI CTAG 1 cut(s) 1182
MalI GATC 8 cut(s) 37, 318, 377, 601, 900, 1137, 1317, 1423
MboI GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
MflI RGATCY 1 cut(s) 599
MhlI GDGCHC 4 cut(s) 411, 565, 1015, 1106
MluCI AATT 5 cut(s) 142, 954, 1150, 1162, 1254
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 1 cut(s) 1065
MroNI GCCGGC 1 cut(s) 275
MseI TTAA 6 cut(s) 11, 254, 630, 648, 1200, 1323
MspI CCGG 5 cut(s) 276, 289, 308, 794, 1051
MspR9I CCNGG 1 cut(s) 795
MssI GTTTAAAC 1 cut(s) 1201
MwoI GCNNNNNNNGC 3 cut(s) 301, 574, 812
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 1 cut(s) 795
NcoI CCATGG 2 cut(s) 435, 1270
NdeI CATATG 1 cut(s) 754
NdeII GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 9 cut(s) 42, 250, 401, 439, 519, 887, 1274, 1307, 1470
NlaIV GGNNCC 4 cut(s) 44, 71, 366, 999
NspI RCATGY 2 cut(s) 401, 1470
PagI TCATGA 1 cut(s) 1303
PdiI GCCGGC 1 cut(s) 277
PfeI GAWTC 6 cut(s) 264, 710, 809, 887, 1045, 1187
PflMI CCANNNNNTGG 3 cut(s) 247, 328, 672
PkrI GCNGC 1 cut(s) 306
PleI GAGTC 1 cut(s) 778
PmeI GTTTAAAC 1 cut(s) 1201
PpsI GAGTC 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 69
PspN4I GGNNCC 4 cut(s) 44, 71, 366, 999
PspPI GGNCC 3 cut(s) 69, 791, 1155
PspPPI RGGWCCY 1 cut(s) 69
PstI CTGCAG 3 cut(s) 662, 1345, 1420
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 599
RsaI GTAC 2 cut(s) 215, 606
RsaNI GTAC 2 cut(s) 214, 605
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 6 cut(s) 11, 254, 630, 648, 1200, 1323
SatI GCNGC 1 cut(s) 305
Sau3AI GATC 8 cut(s) 35, 316, 375, 599, 898, 1135, 1315, 1421
Sau96I GGNCC 3 cut(s) 69, 791, 1155
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 1 cut(s) 795
SduI GDGCHC 4 cut(s) 411, 565, 1015, 1106
SfaNI GCATC 3 cut(s) 1078, 1086, 1252
SfcI CTRYAG 3 cut(s) 658, 1341, 1416
SinI GGWCC 3 cut(s) 69, 791, 1155
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 5 cut(s) 142, 954, 1150, 1162, 1254
SsiI CCGC 2 cut(s) 156, 305
SspI AATATT 1 cut(s) 1300
SspMI CTAG 1 cut(s) 1182
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 793
StyI CCWWGG 2 cut(s) 435, 1270
TaaI ACNGT 4 cut(s) 227, 670, 1206, 1381
TaqI TCGA 3 cut(s) 315, 484, 1021
TasI AATT 5 cut(s) 142, 954, 1150, 1162, 1254
TatI WGTACW 1 cut(s) 604
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 6 cut(s) 264, 710, 809, 887, 1045, 1187
Tru1I TTAA 6 cut(s) 11, 254, 630, 648, 1200, 1323
Tru9I TTAA 6 cut(s) 11, 254, 630, 648, 1200, 1323
TscAI CASTG 4 cut(s) 232, 565, 673, 850
TspDTI ATGAA 6 cut(s) 17, 666, 741, 900, 1106, 1292
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 4 cut(s) 232, 565, 673, 850
Van91I CCANNNNNTGG 3 cut(s) 247, 328, 672
VneI GTGCAC 2 cut(s) 561, 1011
VpaK11BI GGWCC 3 cut(s) 69, 791, 1155
XagI CCTNNNNNAGG 2 cut(s) 64, 855
XceI RCATGY 2 cut(s) 401, 1470
XcmI CCANNNNNNNNNTGG 1 cut(s) 1165
XmiI GTMKAC 1 cut(s) 777
XspI CTAG 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.