FvH4_5g19770

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005420
Physical Location & Seq
Reverse (-)
11605914 .. 11611042
5129 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19770.t6

Sequence Viewer

Length: 705 bp
ATGCGGTTGTTCCCTTTGAACTCGACCCCGAGCTCGAGCTCCAGCTCCACCGTAGAGAAGGAGCAGACTGATCGGAAAAGGACTCGCGCCCTTTTGTACCTCAATGTCTACGACCTCACCCCTGTAAATAACTACCTTTACTGGTTCGGATTCGGGGTCTTTCACTCCGGCATCGAAGTGCATGGCTTGGAGTATGGCTTTGGAGCCCATGAGTATCCCAGCAGTGGAGTTTTTGAGGTGGAACCAAGGAGTTGTCCTGGCTTCATATTCAGAAAGTCACTCCTACTGGGCAGCACTGATATGTCTCGTACAGAATTTCGGTCATTTATGGAGCACCTTTCTGGAAAGTATCATGGTGATACCTATCATTTAATTGCCAAGAATTGCAACCATTTCACTGATGAAGTCTGTTTGCGGCTAACCGGAAAACCTATCCCTGGATGGGTTAATCGGTTGGCACGAGTAGTTTCAGGTTCTTTCTGTAATTGTCTTCTGCCAGAAAACATTCAATTATCAGCAGTCAGACATCTTCCTGATCATCCGCCATATTCTGAAGAAGAAGAAGATGATGATGGATCAGAGTCAATTGCTTCATCTGGAACAGGAAGTGATGAGGATTCAAATCATCACCTGTTGGCTGCACCAATTGGTGATGTAGCTTTCGTGAAGGAAACACCAGTGAGGTTAGCGAGGGAGCTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

235

Amino Acids

26.21

Weight (kDa)

5.52

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 33 - 166 5.2e-52 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 87
AciI CCGC 3 cut(s) 4, 415, 542
AclWI GGATC 1 cut(s) 583
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 573
AfaI GTAC 2 cut(s) 98, 310
AfiI CCNNNNNNNGG 3 cut(s) 224, 437, 442
AgsI TTSAA 3 cut(s) 19, 509, 621
AjnI CCWGG 2 cut(s) 256, 436
AluBI AGCT 5 cut(s) 33, 39, 45, 659, 697
AluI AGCT 5 cut(s) 33, 39, 45, 659, 697
Alw21I GWGCWC 4 cut(s) 35, 41, 336, 699
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 1 cut(s) 583
Ama87I CYCGRG 2 cut(s) 28, 34
ApeKI GCWGC 2 cut(s) 291, 638
ApoI RAATTY 1 cut(s) 314
Asp700I GAANNNNTTC 1 cut(s) 504
AspLEI GCGC 1 cut(s) 89
AsuHPI GGTGA 4 cut(s) 109, 368, 620, 662
AvaI CYCGRG 2 cut(s) 28, 34
BanII GRGCYC 4 cut(s) 35, 41, 208, 699
BauI CACGAG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 482
Bbv12I GWGCWC 4 cut(s) 35, 41, 336, 699
BbvI GCAGC 2 cut(s) 303, 625
BccI CCATC 2 cut(s) 435, 566
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 2 cut(s) 258, 438
BciVI GTATCC 1 cut(s) 225
BclI TGATCA 1 cut(s) 535
BcoDI GTCTC 1 cut(s) 309
BfuI GTATCC 1 cut(s) 225
BisI GCNGC 3 cut(s) 292, 416, 639
BlsI GCNGC 3 cut(s) 293, 417, 640
Bme1390I CCNGG 2 cut(s) 258, 438
BmeT110I CYCGRG 2 cut(s) 28, 34
BmiI GGNNCC 2 cut(s) 205, 243
BmrFI CCNGG 2 cut(s) 258, 438
BmrI ACTGGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 296
BpiI GAAGAC 1 cut(s) 482
BpmI CTGGAG 1 cut(s) 25
BsaBI GATNNNNATC 2 cut(s) 363, 621
BsaJI CCNNGG 2 cut(s) 245, 436
BsaWI WCCGGW 1 cut(s) 422
BsaXI ACNNNNNCTCC 1 cut(s) 686
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 437, 442
Bse1I ACTGG 3 cut(s) 146, 291, 677
Bse8I GATNNNNATC 2 cut(s) 363, 621
BseBI CCWGG 2 cut(s) 258, 438
BseDI CCNNGG 2 cut(s) 245, 436
BseGI GGATG 2 cut(s) 446, 538
BseJI GATNNNNATC 2 cut(s) 363, 621
BseLI CCNNNNNNNGG 3 cut(s) 224, 437, 442
BseNI ACTGG 3 cut(s) 146, 291, 677
BseXI GCAGC 2 cut(s) 303, 625
BseYI CCCAGC 1 cut(s) 218
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 1 cut(s) 87
BsiHKAI GWGCWC 4 cut(s) 35, 41, 336, 699
BsiHKCI CYCGRG 2 cut(s) 28, 34
BsiSI CCGG 2 cut(s) 168, 423
BslI CCNNNNNNNGG 3 cut(s) 224, 437, 442
BsmAI GTCTC 1 cut(s) 309
BsoBI CYCGRG 2 cut(s) 28, 34
Bsp1286I GDGCHC 5 cut(s) 35, 41, 208, 336, 699
Bsp143I GATC 3 cut(s) 70, 535, 575
BspACI CCGC 3 cut(s) 4, 415, 542
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 2 cut(s) 205, 243
BspPI GGATC 1 cut(s) 583
BsrI ACTGG 3 cut(s) 146, 291, 677
BssECI CCNNGG 2 cut(s) 245, 436
BssMI GATC 3 cut(s) 70, 535, 575
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 459
Bst2UI CCWGG 2 cut(s) 258, 438
Bst4CI ACNGT 1 cut(s) 52
BstF5I GGATG 2 cut(s) 446, 538
BstFNI CGCG 1 cut(s) 87
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 3 cut(s) 73, 538, 578
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 3 cut(s) 70, 535, 575
BstNI CCWGG 2 cut(s) 258, 438
BstSCI CCNGG 2 cut(s) 256, 436
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 303, 625
BstV2I GAAGAC 1 cut(s) 482
BsuI GTATCC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 446, 538
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 4 cut(s) 229, 294, 396, 684
CfoI GCGC 1 cut(s) 89
Csp6I GTAC 2 cut(s) 97, 309
CviAII CATG 4 cut(s) 182, 209, 353, 700
CviQI GTAC 2 cut(s) 97, 309
DpnI GATC 3 cut(s) 72, 537, 577
DpnII GATC 3 cut(s) 70, 535, 575
EciI GGCGGA 1 cut(s) 531
Ecl136II GAGCTC 3 cut(s) 33, 39, 697
Eco130I CCWWGG 1 cut(s) 245
Eco24I GRGCYC 4 cut(s) 35, 41, 208, 699
Eco53kI GAGCTC 3 cut(s) 33, 39, 697
Eco57I CTGAAG 1 cut(s) 573
Eco88I CYCGRG 2 cut(s) 28, 34
EcoICRI GAGCTC 3 cut(s) 33, 39, 697
EcoRII CCWGG 2 cut(s) 256, 436
EcoT14I CCWWGG 1 cut(s) 245
EcoT38I GRGCYC 4 cut(s) 35, 41, 208, 699
ErhI CCWWGG 1 cut(s) 245
FaeI CATG 4 cut(s) 185, 212, 356, 703
FaiI YATR 9 cut(s) 183, 195, 210, 266, 302, 329, 354, 547, 701
FatI CATG 4 cut(s) 181, 208, 352, 699
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 3 cut(s) 292, 416, 639
FokI GGATG 2 cut(s) 453, 525
FriOI GRGCYC 4 cut(s) 35, 41, 208, 699
Fsp4HI GCNGC 3 cut(s) 292, 416, 639
GlaI GCGC 1 cut(s) 88
GluI GCNGC 3 cut(s) 292, 416, 639
GsaI CCCAGC 1 cut(s) 222
GsuI CTGGAG 1 cut(s) 25
HapII CCGG 2 cut(s) 168, 423
HhaI GCGC 1 cut(s) 89
Hin1II CATG 4 cut(s) 185, 212, 356, 703
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HinfI GANTC 4 cut(s) 82, 150, 581, 617
HpaII CCGG 2 cut(s) 168, 423
HphI GGTGA 4 cut(s) 109, 368, 620, 662
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 6 cut(s) 75, 149, 272, 524, 553, 580
Hpy188III TCNNGA 4 cut(s) 342, 533, 597, 664
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 52, 661
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4V TGCA 3 cut(s) 181, 387, 641
Hsp92II CATG 4 cut(s) 185, 212, 356, 703
HspAI GCGC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 3 cut(s) 70, 535, 575
LmnI GCTCC 6 cut(s) 44, 50, 61, 203, 331, 694
Lsp1109I GCAGC 2 cut(s) 303, 625
LweI GCATC 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 276
MalI GATC 3 cut(s) 72, 537, 577
MboI GATC 3 cut(s) 70, 535, 575
MboII GAAGA 6 cut(s) 482, 521, 566, 569, 572, 575
MfeI CAATTG 2 cut(s) 585, 645
MhlI GDGCHC 5 cut(s) 35, 41, 208, 336, 699
MluCI AATT 7 cut(s) 314, 372, 382, 484, 509, 585, 645
MlyI GAGTC 2 cut(s) 76, 590
MnlI CCTC 6 cut(s) 110, 125, 229, 607, 675, 684
MroXI GAANNNNTTC 1 cut(s) 504
MseI TTAA 2 cut(s) 371, 447
MslI CAYNNNNRTG 2 cut(s) 176, 299
MspI CCGG 2 cut(s) 168, 423
MspR9I CCNGG 2 cut(s) 258, 438
MunI CAATTG 2 cut(s) 585, 645
MvaI CCWGG 2 cut(s) 258, 438
MvnI CGCG 1 cut(s) 87
NdeII GATC 3 cut(s) 70, 535, 575
NlaIII CATG 4 cut(s) 185, 212, 356, 703
NlaIV GGNNCC 2 cut(s) 205, 243
NmuCI GTSAC 1 cut(s) 276
PaeR7I CTCGAG 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 457
PdmI GAANNNNTTC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 150, 617
PkrI GCNGC 3 cut(s) 293, 417, 640
PleI GAGTC 2 cut(s) 76, 589
PpsI GAGTC 2 cut(s) 76, 589
Psp124BI GAGCTC 3 cut(s) 35, 41, 699
Psp6I CCWGG 2 cut(s) 256, 436
PspFI CCCAGC 1 cut(s) 218
PspGI CCWGG 2 cut(s) 256, 436
PspN4I GGNNCC 2 cut(s) 205, 243
PspXI VCTCGAGB 1 cut(s) 34
RsaI GTAC 2 cut(s) 98, 310
RsaNI GTAC 2 cut(s) 97, 309
RseI CAYNNNNRTG 2 cut(s) 176, 299
SacI GAGCTC 3 cut(s) 35, 41, 699
SaqAI TTAA 2 cut(s) 371, 447
SatI GCNGC 3 cut(s) 292, 416, 639
Sau3AI GATC 3 cut(s) 70, 535, 575
SchI GAGTC 2 cut(s) 76, 590
ScrFI CCNGG 2 cut(s) 258, 438
SduI GDGCHC 5 cut(s) 35, 41, 208, 336, 699
SfaNI GCATC 1 cut(s) 180
Sfr274I CTCGAG 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 34
SmiMI CAYNNNNRTG 2 cut(s) 176, 299
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 7 cut(s) 314, 372, 382, 484, 509, 585, 645
SsiI CCGC 3 cut(s) 4, 415, 542
SstI GAGCTC 3 cut(s) 35, 41, 699
StyD4I CCNGG 2 cut(s) 256, 436
StyI CCWWGG 1 cut(s) 245
TaaI ACNGT 1 cut(s) 52
TaqI TCGA 3 cut(s) 23, 35, 174
TaqII GACCGA 1 cut(s) 309
TasI AATT 7 cut(s) 314, 372, 382, 484, 509, 585, 645
TauI GCSGC 1 cut(s) 418
TfiI GAWTC 2 cut(s) 150, 617
Tru1I TTAA 2 cut(s) 371, 447
Tru9I TTAA 2 cut(s) 371, 447
TscAI CASTG 4 cut(s) 229, 301, 403, 684
TseFI GTSAC 1 cut(s) 276
TseI GCWGC 2 cut(s) 291, 638
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 3 cut(s) 253, 417, 582
TspRI CASTG 4 cut(s) 229, 301, 403, 684
XapI RAATTY 1 cut(s) 314
XhoI CTCGAG 1 cut(s) 34
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.