MD06G1052200.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005420
Physical Location & Seq
Forward (+)
7515868 .. 7523692
7825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1052200.v1.1.491

Sequence Viewer

Length: 675 bp
ATGCGGTTGTTTCCTTTGAGCTCAAGCCCGGGCTCGACCTCCAGCGCAGACAAGGAGCTCATCGATCGGGAAAAAAATCGCGCCCTTTTGTACCTCAATGTCTACGATCTCACGCCTGTGAACAACTACCTTTACTGGGTTGGATTCGGGATCTTCCACTCCGGCATAGAAGTTCATGGCATGGAGTATGGCTTTGGAGCCCATGAGTATCCCAGCAGTGGGGTTTTTGAGGTGGAACCAAGAAGTTGTCCTGGCTTCATCTTCAGAAGGTCAGTCCTGTTGGGAAGCACTGAAATGTCTCGCTCAGAATTTCGGTCATTTATGGAGCATCTTTCTGGAAAGTATCATGGAGATACCTATCATTTAATTGCCAAGAATTGCAACCATTTCACTGATGAAGTTTGTATGCAGCTAACTGGAAAACCTATACCTGGATGGGTAAATCGGCTGGCCCAAGTAGGTTCTTTCTGCAACTGTCTTCTCCCAGAAAACATTCAAATTTCAGCAGTGAGACATCTTCCTGACCATCCAGCATATTCTGACGAAGATGACGACGATGGATCAGAATCTATTGCATCATCTGGAACAGGAGCAAGTGAGGAGGGGCAAAATCATCATCTCCTGACTGTACCAAGTGGTGATGTAGCTTTCGTGAAGGAAAAACCTCCGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

225

Amino Acids

24.85

Weight (kDa)

5.28

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 31 - 164 7.6e-53 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 81
AciI CCGC 1 cut(s) 4
AclWI GGATC 2 cut(s) 158, 568
AcsI RAATTY 2 cut(s) 308, 498
AcuI CTGAAG 1 cut(s) 247
AfaI GTAC 2 cut(s) 92, 630
AfiI CCNNNNNNNGG 4 cut(s) 136, 218, 219, 431
AgsI TTSAA 1 cut(s) 497
AjiI CACGTC 1 cut(s) 672
AjnI CCWGG 2 cut(s) 250, 430
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 4 cut(s) 21, 58, 412, 647
AluI AGCT 4 cut(s) 21, 58, 412, 647
Alw21I GWGCWC 2 cut(s) 23, 60
Alw26I GTCTC 2 cut(s) 303, 505
AlwI GGATC 2 cut(s) 158, 568
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 1 cut(s) 450
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 2 cut(s) 308, 498
Asp700I GAANNNNTTC 1 cut(s) 492
AspLEI GCGC 2 cut(s) 47, 83
AspS9I GGNCC 1 cut(s) 451
AsuC2I CCSGG 2 cut(s) 29, 30
AsuHPI GGTGA 1 cut(s) 650
AvaI CYCGRG 1 cut(s) 28
BanII GRGCYC 4 cut(s) 23, 35, 60, 202
BbsI GAAGAC 1 cut(s) 470
Bbv12I GWGCWC 2 cut(s) 23, 60
BbvI GCAGC 1 cut(s) 421
BccI CCATC 3 cut(s) 429, 534, 551
BciT130I CCWGG 2 cut(s) 252, 432
BciVI GTATCC 1 cut(s) 219
BcnI CCSGG 2 cut(s) 29, 30
BcoDI GTCTC 2 cut(s) 303, 505
BfuI GTATCC 1 cut(s) 219
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
Bme1390I CCNGG 4 cut(s) 29, 30, 252, 432
BmeT110I CYCGRG 1 cut(s) 28
BmgBI CACGTC 1 cut(s) 672
BmgT120I GGNCC 1 cut(s) 451
BmiI GGNNCC 2 cut(s) 199, 237
BmrFI CCNGG 4 cut(s) 29, 30, 252, 432
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 2 cut(s) 337, 584
BmuI ACTGGG 1 cut(s) 145
BpiI GAAGAC 1 cut(s) 470
BpmI CTGGAG 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 7
BpuMI CCSGG 2 cut(s) 29, 30
Bsa29I ATCGAT 1 cut(s) 63
BsaBI GATNNNNATC 2 cut(s) 357, 565
BsaJI CCNNGG 1 cut(s) 28
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 218, 219, 431
Bse1I ACTGG 2 cut(s) 140, 421
Bse8I GATNNNNATC 2 cut(s) 357, 565
BseBI CCWGG 2 cut(s) 252, 432
BseCI ATCGAT 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 2 cut(s) 440, 526
BseJI GATNNNNATC 2 cut(s) 357, 565
BseLI CCNNNNNNNGG 4 cut(s) 136, 218, 219, 431
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 2 cut(s) 140, 421
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 1 cut(s) 421
BseYI CCCAGC 1 cut(s) 212
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 1 cut(s) 452
BshVI ATCGAT 1 cut(s) 63
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 2 cut(s) 23, 60
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 2 cut(s) 29, 162
BslI CCNNNNNNNGG 4 cut(s) 136, 218, 219, 431
BsmAI GTCTC 2 cut(s) 303, 505
BsnI GGCC 1 cut(s) 452
BsoBI CYCGRG 1 cut(s) 28
Bsp1286I GDGCHC 4 cut(s) 23, 35, 60, 202
Bsp143I GATC 4 cut(s) 64, 106, 150, 560
BspACI CCGC 1 cut(s) 4
BspANI GGCC 1 cut(s) 452
BspCNI CTCAG 1 cut(s) 317
BspDI ATCGAT 1 cut(s) 63
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 199, 237
BspPI GGATC 2 cut(s) 158, 568
BsrI ACTGG 2 cut(s) 140, 421
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 4 cut(s) 64, 106, 150, 560
Bst2UI CCWGG 2 cut(s) 252, 432
Bst4CI ACNGT 2 cut(s) 476, 628
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 304
BstF5I GGATG 2 cut(s) 440, 526
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 2 cut(s) 47, 83
BstKTI GATC 4 cut(s) 67, 109, 153, 563
BstMAI GTCTC 2 cut(s) 303, 505
BstMBI GATC 4 cut(s) 64, 106, 150, 560
BstMCI CGRYCG 1 cut(s) 67
BstNI CCWGG 2 cut(s) 252, 432
BstSCI CCNGG 4 cut(s) 27, 28, 250, 430
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
Bsu15I ATCGAT 1 cut(s) 63
BsuI GTATCC 1 cut(s) 219
BsuRI GGCC 1 cut(s) 452
BsuTUI ATCGAT 1 cut(s) 63
BtrI CACGTC 1 cut(s) 672
BtsCI GGATG 2 cut(s) 440, 526
BtsI GCAGTG 2 cut(s) 223, 513
BtsIMutI CAGTG 4 cut(s) 223, 288, 390, 513
Cac8I GCNNGC 1 cut(s) 450
CfoI GCGC 2 cut(s) 47, 83
Cfr13I GGNCC 1 cut(s) 451
Cfr9I CCCGGG 1 cut(s) 28
ClaI ATCGAT 1 cut(s) 63
Csp6I GTAC 2 cut(s) 91, 629
CviAII CATG 4 cut(s) 176, 181, 203, 347
CviQI GTAC 2 cut(s) 91, 629
DdeI CTNAG 1 cut(s) 304
DpnI GATC 4 cut(s) 66, 108, 152, 562
DpnII GATC 4 cut(s) 64, 106, 150, 560
Ecl136II GAGCTC 2 cut(s) 21, 58
Eco24I GRGCYC 4 cut(s) 23, 35, 60, 202
Eco53kI GAGCTC 2 cut(s) 21, 58
Eco57I CTGAAG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 28
EcoICRI GAGCTC 2 cut(s) 21, 58
EcoRII CCWGG 2 cut(s) 250, 430
EcoT38I GRGCYC 4 cut(s) 23, 35, 60, 202
FaeI CATG 4 cut(s) 179, 184, 206, 350
FatI CATG 4 cut(s) 175, 180, 202, 346
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 2 cut(s) 447, 513
FriOI GRGCYC 4 cut(s) 23, 35, 60, 202
Fsp4HI GCNGC 1 cut(s) 410
GlaI GCGC 2 cut(s) 46, 82
GluI GCNGC 1 cut(s) 410
GsaI CCCAGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 25
HaeIII GGCC 1 cut(s) 452
HapII CCGG 2 cut(s) 29, 162
HhaI GCGC 2 cut(s) 47, 83
Hin1II CATG 4 cut(s) 179, 184, 206, 350
Hin6I GCGC 2 cut(s) 45, 81
HinP1I GCGC 2 cut(s) 45, 81
HinfI GANTC 2 cut(s) 144, 566
HpaII CCGG 2 cut(s) 29, 162
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 2 cut(s) 103, 121
Hpy188I TCNGA 5 cut(s) 266, 307, 541, 565, 669
Hpy188III TCNNGA 7 cut(s) 68, 148, 336, 521, 582, 622, 652
Hpy8I GTNNAC 2 cut(s) 103, 121
Hpy99I CGWCG 2 cut(s) 557, 673
HpyAV CCTTC 2 cut(s) 261, 649
HpyCH4III ACNGT 2 cut(s) 476, 628
HpyCH4IV ACGT 1 cut(s) 671
HpyCH4V TGCA 4 cut(s) 381, 409, 471, 575
HpyF3I CTNAG 1 cut(s) 304
HpySE526I ACGT 1 cut(s) 671
Hsp92II CATG 4 cut(s) 179, 184, 206, 350
HspAI GCGC 2 cut(s) 45, 81
Kzo9I GATC 4 cut(s) 64, 106, 150, 560
LmnI GCTCC 4 cut(s) 55, 197, 325, 590
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 2 cut(s) 337, 584
MaeII ACGT 1 cut(s) 671
MalI GATC 4 cut(s) 66, 108, 152, 562
MboI GATC 4 cut(s) 64, 106, 150, 560
MboII GAAGA 5 cut(s) 145, 253, 470, 509, 557
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 4 cut(s) 23, 35, 60, 202
MluCI AATT 4 cut(s) 308, 366, 376, 498
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 6 cut(s) 49, 104, 223, 592, 595, 675
MroXI GAANNNNTTC 1 cut(s) 492
MseI TTAA 1 cut(s) 365
MslI CAYNNNNRTG 2 cut(s) 116, 293
MspI CCGG 2 cut(s) 29, 162
MspR9I CCNGG 4 cut(s) 29, 30, 252, 432
MvaI CCWGG 2 cut(s) 252, 432
MvnI CGCG 1 cut(s) 81
NciI CCSGG 2 cut(s) 29, 30
NdeII GATC 4 cut(s) 64, 106, 150, 560
NlaIII CATG 4 cut(s) 179, 184, 206, 350
NlaIV GGNNCC 2 cut(s) 199, 237
OliI CACNNNNGTG 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 2 cut(s) 144, 566
PkrI GCNGC 1 cut(s) 411
Ple19I CGATCG 1 cut(s) 67
Psp124BI GAGCTC 2 cut(s) 23, 60
Psp6I CCWGG 2 cut(s) 250, 430
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 2 cut(s) 250, 430
PspN4I GGNNCC 2 cut(s) 199, 237
PspPI GGNCC 1 cut(s) 451
PsuI RGATCY 1 cut(s) 150
PvuI CGATCG 1 cut(s) 67
RsaI GTAC 2 cut(s) 92, 630
RsaNI GTAC 2 cut(s) 91, 629
RseI CAYNNNNRTG 2 cut(s) 116, 293
SacI GAGCTC 2 cut(s) 23, 60
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 4 cut(s) 64, 106, 150, 560
Sau96I GGNCC 1 cut(s) 451
ScrFI CCNGG 4 cut(s) 29, 30, 252, 432
SduI GDGCHC 4 cut(s) 23, 35, 60, 202
SfaNI GCATC 2 cut(s) 337, 584
SmaI CCCGGG 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 116, 293
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
SrfI GCCCGGGC 1 cut(s) 30
Sse9I AATT 4 cut(s) 308, 366, 376, 498
SsiI CCGC 1 cut(s) 4
SstI GAGCTC 2 cut(s) 23, 60
StyD4I CCNGG 4 cut(s) 27, 28, 250, 430
TaaI ACNGT 2 cut(s) 476, 628
TaiI ACGT 1 cut(s) 674
TaqI TCGA 2 cut(s) 35, 63
TaqII GACCGA 1 cut(s) 303
TasI AATT 4 cut(s) 308, 366, 376, 498
TfiI GAWTC 2 cut(s) 144, 566
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TscAI CASTG 4 cut(s) 223, 295, 397, 513
TseI GCWGC 1 cut(s) 409
TspDTI ATGAA 3 cut(s) 164, 247, 411
TspMI CCCGGG 1 cut(s) 28
TspRI CASTG 4 cut(s) 223, 295, 397, 513
XapI RAATTY 2 cut(s) 308, 498
XmaI CCCGGG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.