MD04G1058600.v1.1

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005420
Physical Location & Seq
Forward (+)
7642458 .. 7648166
5709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1058600.v1.1.491

Sequence Viewer

Length: 678 bp
ATGCGGTTGTTTCCTTTGAGCTCAAGCCCAAGCTCGACCGCCAGAGCAGACAAGGAGCTCACTGATCGGAAAAAAAATCGCGCCCTTTTGTACCTCAATGTCTACGATCTCACGCCTGTGAACAACTACCTTTACTGGGTTGGATTCGGGATCTTCCACTCCGGCATAGAAGTTCATGGCTTGGAGTATGGCTTTGGAGCCCATGAGTACCCCAGCAGTGGGGTTTTTGAGGTAGAACCAAGAAGTTGTCCTGGCTTCATCTTCAGAAAGTCCGTCCTGGTGGGCAGCACTGATATGTCTCGCTCAGAATTCCGGTCGTTTATGGAGAACCTTTCTGGAAAGTATCATGGAGATACCTATCATTTAATTGCCAAGAATTGCAACCATTTCACTGATGAAGTTTGTATGCGGCTAACTGGAAAAACTATACCTGGATGGGTAAATCGGCTAGCCCGAGTAGGTTCTTTCTGCAACTGTCTTCTTCCAGTTAACATTCAAATTTCAGCAGTGAGACATCTTCCTGACCACCCGACATATTCTGACGAGGACGGTGACGACGGATCAGAATCTATTGTATCATCTGGAACAGCAGCAAGTGAGGAGGGGCCAAATCATCATCTCCTGAATGTACCGAGTGGTGATGTAGCTTTCGTGAAGGAAAAACCTCCGAGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

226

Amino Acids

25.02

Weight (kDa)

6.19

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 31 - 163 4.3e-52 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 102
AccII CGCG 1 cut(s) 81
AciI CCGC 3 cut(s) 4, 39, 409
AclWI GGATC 2 cut(s) 158, 568
AcsI RAATTY 2 cut(s) 308, 498
AcuI CTGAAG 1 cut(s) 247
AfaI GTAC 3 cut(s) 92, 209, 630
AfiI CCNNNNNNNGG 3 cut(s) 136, 218, 219
AgsI TTSAA 1 cut(s) 497
AjnI CCWGG 3 cut(s) 250, 276, 430
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 4 cut(s) 21, 33, 58, 647
AluI AGCT 4 cut(s) 21, 33, 58, 647
Alw21I GWGCWC 2 cut(s) 23, 60
Alw26I GTCTC 2 cut(s) 303, 505
AlwI GGATC 2 cut(s) 158, 568
Ama87I CYCGRG 1 cut(s) 453
AoxI GGCC 1 cut(s) 605
ApeKI GCWGC 2 cut(s) 285, 590
ApoI RAATTY 2 cut(s) 308, 498
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 605
AsuHPI GGTGA 2 cut(s) 563, 650
AsuNHI GCTAGC 1 cut(s) 448
AvaI CYCGRG 1 cut(s) 453
BanII GRGCYC 3 cut(s) 23, 60, 202
BbsI GAAGAC 1 cut(s) 470
Bbv12I GWGCWC 2 cut(s) 23, 60
BbvI GCAGC 2 cut(s) 297, 602
BccI CCATC 1 cut(s) 429
BciT130I CCWGG 3 cut(s) 252, 278, 432
BcoDI GTCTC 2 cut(s) 303, 505
BfaI CTAG 1 cut(s) 449
BisI GCNGC 3 cut(s) 286, 410, 591
BlsI GCNGC 3 cut(s) 287, 411, 592
Bme1390I CCNGG 3 cut(s) 252, 278, 432
BmeT110I CYCGRG 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 2 cut(s) 199, 606
BmrFI CCNGG 3 cut(s) 252, 278, 432
BmrI ACTGGG 1 cut(s) 145
BmtI GCTAGC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 145
BpiI GAAGAC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 7
BsaBI GATNNNNATC 2 cut(s) 357, 565
BsaJI CCNNGG 1 cut(s) 668
BsaWI WCCGGW 1 cut(s) 312
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 218, 219
Bse1I ACTGG 3 cut(s) 140, 421, 485
Bse8I GATNNNNATC 2 cut(s) 357, 565
BseBI CCWGG 3 cut(s) 252, 278, 432
BseDI CCNNGG 1 cut(s) 668
BseGI GGATG 1 cut(s) 440
BseJI GATNNNNATC 2 cut(s) 357, 565
BseLI CCNNNNNNNGG 3 cut(s) 136, 218, 219
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 3 cut(s) 140, 421, 485
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 2 cut(s) 297, 602
BseYI CCCAGC 1 cut(s) 212
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 2 cut(s) 39, 317
BshFI GGCC 1 cut(s) 607
BsiEI CGRYCG 2 cut(s) 39, 317
BsiHKAI GWGCWC 2 cut(s) 23, 60
BsiHKCI CYCGRG 1 cut(s) 453
BsiSI CCGG 2 cut(s) 162, 313
BslI CCNNNNNNNGG 3 cut(s) 136, 218, 219
BsmAI GTCTC 2 cut(s) 303, 505
BsnI GGCC 1 cut(s) 607
BsoBI CYCGRG 1 cut(s) 453
Bsp1286I GDGCHC 3 cut(s) 23, 60, 202
Bsp143I GATC 4 cut(s) 64, 106, 150, 560
BspACI CCGC 3 cut(s) 4, 39, 409
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 1 cut(s) 317
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 199, 606
BspOI GCTAGC 1 cut(s) 452
BspPI GGATC 2 cut(s) 158, 568
BsrI ACTGG 3 cut(s) 140, 421, 485
BssECI CCNNGG 1 cut(s) 668
BssMI GATC 4 cut(s) 64, 106, 150, 560
Bst2UI CCWGG 3 cut(s) 252, 278, 432
Bst4CI ACNGT 2 cut(s) 476, 551
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 304
BstF5I GGATG 1 cut(s) 440
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 4 cut(s) 67, 109, 153, 563
BstMAI GTCTC 2 cut(s) 303, 505
BstMBI GATC 4 cut(s) 64, 106, 150, 560
BstMCI CGRYCG 2 cut(s) 39, 317
BstNI CCWGG 3 cut(s) 252, 278, 432
BstSCI CCNGG 3 cut(s) 250, 276, 430
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 2 cut(s) 297, 602
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 1 cut(s) 607
BtsCI GGATG 1 cut(s) 440
BtsI GCAGTG 2 cut(s) 223, 513
BtsIMutI CAGTG 5 cut(s) 60, 223, 288, 390, 513
Cac8I GCNNGC 1 cut(s) 450
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 605
Csp6I GTAC 3 cut(s) 91, 208, 629
CviAII CATG 3 cut(s) 176, 203, 347
CviQI GTAC 3 cut(s) 91, 208, 629
DdeI CTNAG 1 cut(s) 304
DpnI GATC 4 cut(s) 66, 108, 152, 562
DpnII GATC 4 cut(s) 64, 106, 150, 560
Ecl136II GAGCTC 2 cut(s) 21, 58
Eco24I GRGCYC 3 cut(s) 23, 60, 202
Eco53kI GAGCTC 2 cut(s) 21, 58
Eco57I CTGAAG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 453
EcoICRI GAGCTC 2 cut(s) 21, 58
EcoRI GAATTC 1 cut(s) 308
EcoRII CCWGG 3 cut(s) 250, 276, 430
EcoT38I GRGCYC 3 cut(s) 23, 60, 202
FaeI CATG 3 cut(s) 179, 206, 350
FatI CATG 3 cut(s) 175, 202, 346
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 286, 410, 591
FokI GGATG 1 cut(s) 447
FriOI GRGCYC 3 cut(s) 23, 60, 202
Fsp4HI GCNGC 3 cut(s) 286, 410, 591
FspBI CTAG 1 cut(s) 449
GlaI GCGC 1 cut(s) 82
GluI GCNGC 3 cut(s) 286, 410, 591
GsaI CCCAGC 1 cut(s) 216
HaeIII GGCC 1 cut(s) 607
HapII CCGG 2 cut(s) 162, 313
HhaI GCGC 1 cut(s) 83
Hin1II CATG 3 cut(s) 179, 206, 350
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 2 cut(s) 144, 566
HpaI GTTAAC 1 cut(s) 490
HpaII CCGG 2 cut(s) 162, 313
HphI GGTGA 2 cut(s) 563, 650
Hpy166II GTNNAC 3 cut(s) 103, 121, 490
Hpy188I TCNGA 6 cut(s) 69, 266, 307, 541, 565, 669
Hpy188III TCNNGA 6 cut(s) 148, 336, 521, 582, 622, 652
Hpy8I GTNNAC 3 cut(s) 103, 121, 490
Hpy99I CGWCG 1 cut(s) 560
HpyAV CCTTC 1 cut(s) 649
HpyCH4III ACNGT 2 cut(s) 476, 551
HpyCH4V TGCA 2 cut(s) 381, 471
HpyF3I CTNAG 1 cut(s) 304
Hsp92II CATG 3 cut(s) 179, 206, 350
HspAI GCGC 1 cut(s) 81
KspAI GTTAAC 1 cut(s) 490
Kzo9I GATC 4 cut(s) 64, 106, 150, 560
LmnI GCTCC 2 cut(s) 55, 197
Lsp1109I GCAGC 2 cut(s) 297, 602
MaeI CTAG 1 cut(s) 449
MaeIII GTNAC 1 cut(s) 551
MalI GATC 4 cut(s) 66, 108, 152, 562
MboI GATC 4 cut(s) 64, 106, 150, 560
MboII GAAGA 5 cut(s) 145, 253, 470, 473, 509
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 3 cut(s) 23, 60, 202
MluCI AATT 4 cut(s) 308, 366, 376, 498
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 7 cut(s) 104, 223, 538, 592, 595, 663, 675
MseI TTAA 2 cut(s) 365, 489
MslI CAYNNNNRTG 2 cut(s) 116, 293
MspI CCGG 2 cut(s) 162, 313
MspR9I CCNGG 3 cut(s) 252, 278, 432
MvaI CCWGG 3 cut(s) 252, 278, 432
MvnI CGCG 1 cut(s) 81
NdeII GATC 4 cut(s) 64, 106, 150, 560
NheI GCTAGC 1 cut(s) 448
NlaIII CATG 3 cut(s) 179, 206, 350
NlaIV GGNNCC 2 cut(s) 199, 606
NmuCI GTSAC 1 cut(s) 551
OliI CACNNNNGTG 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 451
PfeI GAWTC 2 cut(s) 144, 566
PkrI GCNGC 3 cut(s) 287, 411, 592
Psp124BI GAGCTC 2 cut(s) 23, 60
Psp6I CCWGG 3 cut(s) 250, 276, 430
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 3 cut(s) 250, 276, 430
PspN4I GGNNCC 2 cut(s) 199, 606
PspPI GGNCC 1 cut(s) 605
PsuI RGATCY 1 cut(s) 150
RsaI GTAC 3 cut(s) 92, 209, 630
RsaNI GTAC 3 cut(s) 91, 208, 629
RseI CAYNNNNRTG 2 cut(s) 116, 293
SacI GAGCTC 2 cut(s) 23, 60
SaqAI TTAA 2 cut(s) 365, 489
SatI GCNGC 3 cut(s) 286, 410, 591
Sau3AI GATC 4 cut(s) 64, 106, 150, 560
Sau96I GGNCC 1 cut(s) 605
ScrFI CCNGG 3 cut(s) 252, 278, 432
SduI GDGCHC 3 cut(s) 23, 60, 202
SmiMI CAYNNNNRTG 2 cut(s) 116, 293
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
Sse9I AATT 4 cut(s) 308, 366, 376, 498
SsiI CCGC 3 cut(s) 4, 39, 409
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 2 cut(s) 23, 60
StyD4I CCNGG 3 cut(s) 250, 276, 430
TaaI ACNGT 2 cut(s) 476, 551
TaqI TCGA 1 cut(s) 35
TasI AATT 4 cut(s) 308, 366, 376, 498
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 2 cut(s) 144, 566
Tru1I TTAA 2 cut(s) 365, 489
Tru9I TTAA 2 cut(s) 365, 489
TscAI CASTG 5 cut(s) 67, 223, 295, 397, 513
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 2 cut(s) 285, 590
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 3 cut(s) 164, 247, 411
TspGWI ACGGA 2 cut(s) 262, 573
TspRI CASTG 5 cut(s) 67, 223, 295, 397, 513
XapI RAATTY 2 cut(s) 308, 498
XmiI GTMKAC 1 cut(s) 102
XspI CTAG 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.