FvH4_5g20161

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016345
Physical Location & Seq
Forward (+)
11903020 .. 11903534
515 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20161.t1

Sequence Viewer

Length: 456 bp
ATGTATTTTGAGAAACGTTCATTCGAGAGCGAAGTGTGGCTCACAAGCGACCCCAATCGATGGGAAGGCGAGGACTCGGTTGAGATCAAGATAAAGATCTACACTAAGATGGGAGTCTCCTTGAAACCTGGTAAAACCATGTCTGATGGCATACCTTACCACCATAAAGGTGACGAACTCGTCATCTCTGATCTTCCTCGAAGCGAGCTATTATCCAACAGGAGTGAGTATACATGGAGAATGGTCGACTTCTTTTCACTCCGATTTAAGGAGGAGCCACTCCCCGATCATGTACGACGATCTTGTATTGAAGAAGTATTCCGGGTGGCCGAAAAAGAAGATCCAGCAGTGGCCTCAACAGCTTCTGTCATTCATGTCGACATAGTGGAAGTGAAGGAGTGCTTGTTCACATCTGCTTGCGATGCTTTTCTAGACGATGCGATATCATCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.37

Weight (kDa)

4.87

Isoelectric Point (pI)

64.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 60
AccI GTMKAC 3 cut(s) 230, 246, 378
AclI AACGTT 1 cut(s) 16
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 327
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 60, 268
AgsI TTSAA 2 cut(s) 124, 311
AjnI CCWGG 1 cut(s) 127
AloI GAACNNNNNNTCC 2 cut(s) 389, 421
AluBI AGCT 2 cut(s) 208, 362
AluI AGCT 2 cut(s) 208, 362
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 2 cut(s) 327, 351
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 182
BccI CCATC 3 cut(s) 54, 103, 140
BciT130I CCWGG 1 cut(s) 129
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 1 cut(s) 431
BglII AGATCT 1 cut(s) 96
Bme1390I CCNGG 2 cut(s) 129, 323
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 2 cut(s) 129, 323
BmsI GCATC 2 cut(s) 412, 427
BpuMI CCSGG 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 268
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 1 cut(s) 129
BseCI ATCGAT 1 cut(s) 58
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 60, 268
BseRI GAGGAG 1 cut(s) 287
BshFI GGCC 2 cut(s) 329, 353
BshVI ATCGAT 1 cut(s) 58
BsiSI CCGG 1 cut(s) 322
BslI CCNNNNNNNGG 2 cut(s) 60, 268
BsmAI GTCTC 1 cut(s) 121
BsnI GGCC 2 cut(s) 329, 353
Bsp143I GATC 6 cut(s) 84, 96, 190, 286, 299, 340
BspANI GGCC 2 cut(s) 329, 353
BspDI ATCGAT 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 276
BspPI GGATC 1 cut(s) 335
BssMI GATC 6 cut(s) 84, 96, 190, 286, 299, 340
BssNAI GTATAC 1 cut(s) 231
Bst1107I GTATAC 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 206, 418
BstDEI CTNAG 1 cut(s) 105
BstKTI GATC 6 cut(s) 87, 99, 193, 289, 302, 343
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 6 cut(s) 84, 96, 190, 286, 299, 340
BstMWI GCNNNNNNNGC 2 cut(s) 359, 422
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 2 cut(s) 127, 321
BstX2I RGATCY 2 cut(s) 96, 340
BstYI RGATCY 2 cut(s) 96, 340
BstZ17I GTATAC 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 58
BsuRI GGCC 2 cut(s) 329, 353
BsuTUI ATCGAT 1 cut(s) 58
BtgZI GCGATG 1 cut(s) 435
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 2 cut(s) 206, 418
CaiI CAGNNNCTG 1 cut(s) 365
ClaI ATCGAT 1 cut(s) 58
CsiI ACCWGGT 1 cut(s) 127
Csp6I GTAC 1 cut(s) 293
CviAII CATG 4 cut(s) 139, 234, 290, 374
CviJI RGCY 6 cut(s) 40, 208, 277, 329, 353, 362
CviKI_1 RGCY 6 cut(s) 40, 208, 277, 329, 353, 362
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 1 cut(s) 105
DpnI GATC 6 cut(s) 86, 98, 192, 288, 301, 342
DpnII GATC 6 cut(s) 84, 96, 190, 286, 299, 340
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 327
Eco32I GATATC 1 cut(s) 444
EcoRII CCWGG 1 cut(s) 127
EcoRV GATATC 1 cut(s) 444
FaeI CATG 4 cut(s) 142, 237, 293, 377
FaiI YATR 8 cut(s) 140, 152, 165, 231, 235, 291, 375, 383
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FatI CATG 4 cut(s) 138, 233, 289, 373
FblI GTMKAC 3 cut(s) 230, 246, 378
FspBI CTAG 1 cut(s) 431
HaeIII GGCC 2 cut(s) 329, 353
HapII CCGG 1 cut(s) 322
Hin1II CATG 4 cut(s) 142, 237, 293, 377
HincII GTYRAC 2 cut(s) 247, 379
HindII GTYRAC 2 cut(s) 247, 379
HinfI GANTC 2 cut(s) 74, 114
HpaII CCGG 1 cut(s) 322
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 4 cut(s) 231, 247, 379, 408
Hpy188I TCNGA 3 cut(s) 145, 190, 263
Hpy188III TCNNGA 3 cut(s) 25, 88, 431
Hpy8I GTNNAC 4 cut(s) 231, 247, 379, 408
Hpy99I CGWCG 1 cut(s) 300
HpyAV CCTTC 2 cut(s) 59, 388
HpyCH4IV ACGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 422
HpyF3I CTNAG 1 cut(s) 105
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 4 cut(s) 142, 237, 293, 377
Kzo9I GATC 6 cut(s) 84, 96, 190, 286, 299, 340
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 5 cut(s) 114, 141, 205, 335, 357
LweI GCATC 2 cut(s) 412, 427
MabI ACCWGGT 1 cut(s) 127
MaeI CTAG 1 cut(s) 431
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 170
MalI GATC 6 cut(s) 86, 98, 192, 288, 301, 342
MboI GATC 6 cut(s) 84, 96, 190, 286, 299, 340
MboII GAAGA 3 cut(s) 185, 323, 350
MflI RGATCY 2 cut(s) 96, 340
MlyI GAGTC 2 cut(s) 68, 123
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 4 cut(s) 64, 207, 265, 364
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 107, 168
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 2 cut(s) 129, 323
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 359, 422
NciI CCSGG 1 cut(s) 323
NdeII GATC 6 cut(s) 84, 96, 190, 286, 299, 340
NlaIII CATG 4 cut(s) 142, 237, 293, 377
NlaIV GGNNCC 1 cut(s) 276
NmuCI GTSAC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 60
PleI GAGTC 2 cut(s) 68, 122
PpsI GAGTC 2 cut(s) 68, 122
Psp1406I AACGTT 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 2 cut(s) 96, 340
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
RseI CAYNNNNRTG 2 cut(s) 107, 168
SalI GTCGAC 2 cut(s) 245, 377
SaqAI TTAA 1 cut(s) 267
Sau3AI GATC 6 cut(s) 84, 96, 190, 286, 299, 340
SchI GAGTC 2 cut(s) 68, 123
ScrFI CCNGG 2 cut(s) 129, 323
SetI ASST 6 cut(s) 19, 130, 157, 172, 210, 364
SexAI ACCWGGT 1 cut(s) 127
SfaNI GCATC 2 cut(s) 412, 427
SmiMI CAYNNNNRTG 2 cut(s) 107, 168
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 127, 321
TaiI ACGT 1 cut(s) 19
TaqI TCGA 5 cut(s) 24, 58, 199, 246, 378
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 354
TseFI GTSAC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 2 cut(s) 9, 362
TspRI CASTG 1 cut(s) 354
Van91I CCANNNNNTGG 1 cut(s) 60
XbaI TCTAGA 1 cut(s) 430
XmiI GTMKAC 3 cut(s) 230, 246, 378
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.