FvH4_5g20170

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016345
Physical Location & Seq
Forward (+)
11903538 .. 11905889
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20170.t1

Sequence Viewer

Length: 534 bp
ATGGAGAAAGAGAGAGTTGATGGCTGCTTCTTGGGGTTTGAAGACTCTTGTGTTATTTGTATGGAGGACCTAGTTGATAAAAAAGGCAAGAAACTCAAACAACAACCCAAGAGGAAGAGCAAGAGGCTGCTTGGACTACAAAGCAGCAGTGGCACCATCGCTAAAACGCCAAGAAAAGAGCCACCCCCTTTGAGGGTAGTTGCTCGGTTGCCTTGCTCACACCGTTTCCATGGAGACTGCATTGTCAACTGGCTCAAAAACAATCATGTTTGTCCTCTTTGCCGAAACCAAGTGGTTGGTCAGCCATCGTTAACTAAGCTGTTTCAAGTGTTACAAAAGAAACTAGCCGAATGGAAAGCAAATACTTTGTCTAGGGGTGCTACCAATATTCCTTCTCATAAACCAAGAACTTCACGCTTTCCTATTATCAAATGGCATCTTCCTGCTGCAGATTATATGAAGATTAATTTTGATGTGTCAATTCAATATAATCAATATATGACTTGGAGAGAGAAGCAGGGGAGATTATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

178

Amino Acids

20.49

Weight (kDa)

9.97

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 67 - 95 5.3e-10 Ring finger domain
zf-rbx1 PF12678 67 - 95 4.9e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 69 - 94 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 70 - 94 5.9e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AccB1I GGYRCC 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 192, 193
AgsI TTSAA 3 cut(s) 41, 326, 485
AluBI AGCT 1 cut(s) 319
AluI AGCT 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 228
ApeKI GCWGC 4 cut(s) 24, 127, 144, 446
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AseI ATTAAT 1 cut(s) 465
AspS9I GGNCC 1 cut(s) 67
AvaII GGWCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 48
BbvI GCAGC 4 cut(s) 11, 114, 156, 433
BccI CCATC 3 cut(s) 14, 164, 313
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 3 cut(s) 71, 344, 372
BfmI CTRYAG 1 cut(s) 447
BisI GCNGC 4 cut(s) 25, 128, 145, 447
BlsI GCNGC 4 cut(s) 26, 129, 146, 448
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 154
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 229
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 193
Bse1I ACTGG 1 cut(s) 254
BseDI CCNNGG 1 cut(s) 229
BseLI CCNNNNNNNGG 2 cut(s) 192, 193
BseNI ACTGG 1 cut(s) 254
BseXI GCAGC 4 cut(s) 11, 114, 156, 433
BshNI GGYRCC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 192, 193
BsmAI GTCTC 1 cut(s) 228
Bsp19I CCATGG 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 154
BspMAI CTGCAG 1 cut(s) 451
BspQI GCTCTTC 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 152
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 229
BssT1I CCWWGG 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 110
BstDEI CTNAG 1 cut(s) 315
BstDSI CCRYGG 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 4 cut(s) 11, 114, 156, 433
BstV2I GAAGAC 1 cut(s) 48
BstXI CCANNNNNNTGG 1 cut(s) 296
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 67
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 2 cut(s) 230, 266
CviJI RGCY 7 cut(s) 24, 127, 181, 253, 304, 319, 347
CviKI_1 RGCY 7 cut(s) 24, 127, 181, 253, 304, 319, 347
DdeI CTNAG 1 cut(s) 315
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 229
Eco47I GGWCC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 2 cut(s) 233, 269
FatI CATG 2 cut(s) 229, 265
Fnu4HI GCNGC 4 cut(s) 25, 128, 145, 447
Fsp4HI GCNGC 4 cut(s) 25, 128, 145, 447
FspBI CTAG 3 cut(s) 71, 344, 372
GluI GCNGC 4 cut(s) 25, 128, 145, 447
Hin1II CATG 2 cut(s) 233, 269
HincII GTYRAC 2 cut(s) 247, 312
HindII GTYRAC 2 cut(s) 247, 312
HinfI GANTC 1 cut(s) 44
HpaI GTTAAC 1 cut(s) 312
Hpy166II GTNNAC 2 cut(s) 247, 312
Hpy8I GTNNAC 2 cut(s) 247, 312
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4V TGCA 2 cut(s) 240, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 150
HpyF3I CTNAG 1 cut(s) 315
Hsp92II CATG 2 cut(s) 233, 269
KspAI GTTAAC 1 cut(s) 312
LguI GCTCTTC 1 cut(s) 110
LpnPI CCDG 3 cut(s) 235, 456, 503
Lsp1109I GCAGC 4 cut(s) 11, 114, 156, 433
LweI GCATC 1 cut(s) 445
MaeI CTAG 3 cut(s) 71, 344, 372
MaeIII GTNAC 1 cut(s) 330
MboII GAAGA 4 cut(s) 53, 127, 431, 472
MluCI AATT 2 cut(s) 466, 480
MlyI GAGTC 1 cut(s) 38
MnlI CCTC 5 cut(s) 58, 105, 117, 186, 285
MseI TTAA 2 cut(s) 311, 465
MwoI GCNNNNNNNGC 1 cut(s) 150
NcoI CCATGG 1 cut(s) 229
NlaIII CATG 2 cut(s) 233, 269
NlaIV GGNNCC 1 cut(s) 154
PciSI GCTCTTC 1 cut(s) 110
PkrI GCNGC 4 cut(s) 26, 129, 146, 448
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
PpuMI RGGWCCY 1 cut(s) 67
PshBI ATTAAT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 1 cut(s) 67
PspPPI RGGWCCY 1 cut(s) 67
PstI CTGCAG 1 cut(s) 451
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 2 cut(s) 311, 465
SatI GCNGC 4 cut(s) 25, 128, 145, 447
Sau96I GGNCC 1 cut(s) 67
SchI GAGTC 1 cut(s) 38
SetI ASST 2 cut(s) 72, 321
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 1 cut(s) 67
Sse9I AATT 2 cut(s) 466, 480
SspI AATATT 1 cut(s) 388
SspMI CTAG 3 cut(s) 71, 344, 372
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 224
TasI AATT 2 cut(s) 466, 480
Tru1I TTAA 2 cut(s) 311, 465
Tru9I TTAA 2 cut(s) 311, 465
TscAI CASTG 1 cut(s) 154
TseI GCWGC 4 cut(s) 24, 127, 144, 446
TspDTI ATGAA 1 cut(s) 473
TspRI CASTG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 67
VspI ATTAAT 1 cut(s) 465
XspI CTAG 3 cut(s) 71, 344, 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.